| 质粒名称: | pCMV(CAT)T7-SB100 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 34879 |
| 存储实验室: | Zsuzsanna Izsvak |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 载体抗性: | 氯霉素 |
| 载体类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | SB100X transposase |
| 插入大小: | 1023 bp |
| 启动子 | CMV |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | SpeI(未知是否被破坏) |
| 3' 克隆位点: | ApaI (未知是否被破坏) |
| 5' 测序引物 | CACCAAAATCAACGGGACTT |
|
文献引用方法 |
|
| 材料和方法部分: | pCMV(CAT)T7-SB100 was a gift from Zsuzsanna Izsvak (Addgene plasmid # 34879) |
| 参考文献部分: |
Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust stable gene transfer in vertebrates. Mates L, Chuah MK, Belay E, Jerchow B, Manoj N, Acosta-Sanchez A, Grzela DP, Schmitt A, Becker K, Matrai J, Ma L, Samara-Kuko E, Gysemans C, Pryputniewicz D, Miskey C, Fletcher B, VandenDriessche T, Ivics Z, Izsvak Z. Nat Genet. 2009 Jun;41(6):753-61. Epub 2009 May 3. 10.1038/ng.343 PubMed 19412179 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
T7-SB100质粒图谱.png)
载体简介
载体序列
LOCUS pCMV(CAT)T7-SB100 4752 bp DNA SYN
DEFINITION pCMV(CAT)T7-SB100
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..4752
/organism="pCMV(CAT)T7-SB100"
/mol_type="other DNA"
misc_feature 31..318
/label="CAG_enhancer"
promoter 36..528
/label="CMV_immearly_promoter"
misc_feature 485..505
/label="CMV_fwd_primer"
promoter 486..555
/label="CMV_promoter"
promoter 498..617
/label="CMV2_promoter"
misc_feature 529..548
/label="pCEP_fwd_primer"
misc_feature 531..555
/label="LNCX_primer"
misc_feature 672..768
/label="modSV40_late_16s_int"
misc_feature 672..702
/label="SV40_late_19s_int"
promoter 825..843
/label="T7_promoter"
CDS 854..1876
/label="ORF frame 2"
terminator complement(1903..2022)
/label="SV40_PA_terminator"
misc_feature complement(1917..1936)
/label="EBV_rev_primer"
promoter complement(2134..2152)
/label="M13_reverse_primer"
misc_feature complement(2151..2173)
/label="M13_pUC_rev_primer"
promoter complement(2187..2216)
/label="lac_promoter"
rep_origin complement(2525..3144)
/label="pBR322_origin"
gene complement(3857..4516)
/label="CAT/CamR"
/gene="CAT/CamR"
CDS complement(3857..4516)
/label="ORF frame 3"
ORIGIN
1 GAATTCGAGC TTGCATGCCT GCAGGTCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC
61 TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG
121 CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG
181 GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA
241 TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC
301 ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG
361 CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG
421 AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA
481 TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTCGTTTA
541 GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGACAC
601 CGGGACCGAT CCAGCCTCCG GACTCTAGAG GATCCGGTAC TCGAGGAACT GAAAAACCAG
661 AAAGTTAACT GGTAAGTTTA GTCTTTTTGT CTTTTATTTC AGGTCCCGGA TCCGGTGGTG
721 GTGCAAATCA AAGAACTGCT CCTCAGTGGA TGTTGCCTTT ACTTCTAGGC CTGTACGGAA
781 GTGTTACTTC TGCTCTAAAA GCTGCGGAAT TGTACCCGCG GAATTAATAC GACTCACTAT
841 AGGGACTAGT ACCATGGGAA AATCAAAAGA AATCAGCCAA GACCTCAGAA AAAGAATTGT
901 AGACCTCCAC AAGTCTGGTT CATCCTTGGG AGCAATTTCC AAACGCCTGG CGGTACCACG
961 TTCATCTGTA CAAACAATAG TACGCAAGTA TAAACACCAT GGGACCACGC AGCCGTCATA
1021 CCGCTCAGGA AGGAGACGCG TTCTGTCTCC TAGAGATGAA CGTACTTTGG TGCGAAAAGT
1081 GCAAATCAAT CCCAGAACAA CAGCAAAGGA CCTTGTGAAG ATGCTGGAGG AAACAGGTAC
1141 AAAAGTATCT ATATCCACAG TAAAACGAGT CCTATATCGA CATAACCTGA AAGGCCACTC
1201 AGCAAGGAAG AAGCCACTGC TCCAAAACCG ACATAAGAAA GCCAGACTAC GGTTTGCAAC
1261 TGCACATGGG GACAAAGATC GTACTTTTTG GAGAAATGTC CTCTGGTCTG ATGAAACAAA
1321 AATAGAACTG TTTGGCCATA ATGACCATCG TTATGTTTGG AGGAAGAAGG GGGAGGCTTG
1381 CAAGCCGAAG AACACCATCC CAACCGTGAA GCACGGGGGT GGCAGCATCA TGTTGTGGGG
1441 GTGCTTTGCT GCAGGAGGGA CTGGTGCACT TCACAAAATA GATGGCATCA TGGACGCGGT
1501 GCAGTATGTG GATATATTGA AGCAACATCT CAAGACATCA GTCAGGAAGT TAAAGCTTGG
1561 TCGCAAATGG GTCTTCCAAC ACGACAATGA CCCCAAGCAT ACTTCCAAAG TTGTGGCAAA
1621 ATGGCTTAAG GACAACAAAG TCAAGGTATT GGAGTGGCCA TCACAAAGCC CTGACCTCAA
1681 TCCTATAGAA AATTTGTGGG CAGAACTGAA AAAGCGTGTG CGAGCAAGGA GGCCTACAAA
1741 CCTGACTCAG TTACACCAGC TCTGTCAGGA GGAATGGGCC AAAATTCACC CAAATTATTG
1801 TGGGAAGCTT GTGGAAGGCT ACCCGAAACG TTTGACCCAA GTTAAACAAT TTAAAGGCAA
1861 TGCTACCAAA TACTAGGGGC CCTAACCGCG GGGATCCAGA CATGATAAGA TACATTGATG
1921 AGTTTGGACA AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG
1981 ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC AACAACAATT
2041 GCATTCATTT TATGTTTCAG GTTCAGGGGG AGGTGTGGGA GGTTTTTTCG GATCCTCTAG
2101 AGTCGACCTG CAGGCATGCA AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA
2161 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT
2221 GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC
2281 AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG
2341 GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC
2401 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG
2461 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA
2521 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC
2581 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC
2641 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG
2701 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT
2761 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC
2821 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC
2881 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG
2941 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG
3001 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA
3061 CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG
3121 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT
3181 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA
3241 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT
3301 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG
3361 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA
3421 GTGCTGCAAT GATACCGCTG GATCGATCCA CCATACCCAC GCCGAAACAA GCGCCCTGCA
3481 CCATTATGTT CCGGATCTGC ATCGCAGGAT GCTGCTGGCT ACCCTGTGGA ACACCTACAT
3541 CTGTATTAAC GAAGCGCTAA CCGTTTTTAT CAGGCTCTGG GAGGCAGAAT AAATGATCAT
3601 ATCGTCAATT ATTACCTCCA CGGGGAGAGC CTGAGCAAAC TGGCCTCAGG CATTTGAGAA
3661 GCACACGGTC ACACTGCTTC CGGTAGTCAA TAAACCGGTA AACCAGCAAT AGACATAAGC
3721 GGCTATTTAA CGACCCTGCC CTGAACCGAC GACCGGGTCG AATTTGCTTT CGAATTTCTG
3781 CCATTCATCC GCTTATTATC ACTTATTCAG GCGTAGCACC AGGCGTTTAA GGGCACCAAT
3841 AACTGCCTTA AAAAAATTAC GCCCCGCCCT GCCACTCATC GCAGTACTGT TGTAATTCAT
3901 TAAGCATTCT GCCGACATGG AAGCCATCAC AGACGGCATG ATGAACCTGA ATCGCCAGCG
3961 GCATCAGCAC CTTGTCGCCT TGCGTATAAT ATTTGCCCAT GGTGAAAACG GGGGCGAAGA
4021 AGTTGTCCAT ATTGGCCACG TTTAAATCAA AACTGGTGAA ACTCACCCAG GGATTGGCTG
4081 AGACGAAAAA CATATTCTCA ATAAACCCTT TAGGGAAATA GGCCAGGTTT TCACCGTAAC
4141 ACGCCACATC TTGCGAATAT ATGTGTAGAA ACTGCCGGAA ATCGTCGTGG TATTCACTCC
4201 AGAGCGATGA AAACGTTTCA GTTTGCTCAT GGAAAACGGT GTAACAAGGG TGAACACTAT
4261 CCCATATCAC CAGCTCACCG TCTTTCATTG CCATACGGAA TTCCGGATGA GCATTCATCA
4321 GGCGGGCAAG AATGTGAATA AAGGCCGGAT AAAACTTGTG CTTATTTTTC TTTACGGTCT
4381 TTAAAAAGGC CGTAATATCC AGCTGAACGG TCTGGTTATA GGTACATTGA GCAACTGACT
4441 GAAATGCCTC AAAATGTTCT TTACGATGCC ATTGGGATAT ATCAACGGTG GTATATCCAG
4501 TGATTTTTTT CTCCATTTTA GCTTCCTTAG CTCCTGAAAA TCTCGATAAC TCAAAAAATA
4561 CGCCCGGTAG TGATCTTATT TCATTATGGT GAAAGTTGGA ACCTCTTACG TGCCGATCAA
4621 CGTCTCATTT TCGCCAAAAG TTGGCCCAGG GCTTCCCGGT ATCAACAGGG ACACCAGGAT
4681 TTATTTATTC TGCGAAGTGA TCTTCCGTCA CAGGTATTTA TTCGGCGCAA AGTGCGTCGG
4741 GTCACTACGT GA
//
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载体质粒应用举例
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