| 质粒名称: | pPSU1 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 89439 |
| 存储实验室: | pUC9 |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 载体出品公司: | Clontech |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 2635bp |
| 完整质粒大小: | 10000bp |
| 载体修饰: | 人细胞色素c氧化酶亚基VIII（human cytochrome C oxidase subunit VIII） |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | 500 bp EcoRV fragment |
| 物种来源: | -- |
| 插入大小: | 500 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | 人细胞色素c氧化酶亚基VIII（N端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AgeI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | pPSU1 was a gift from Song Tan (Addgene plasmid # 89439 ; http://n2t.net/addgene:89439 ; RRID:Addgene_89439) |
| 参考文献部分: | The pPSU Plasmids for Generating DNA Molecular Weight Markers. Henrici RC, Pecen TJ, Johnston JL, Tan S. Sci Rep. 2017 May 26;7(1):2438. doi: 10.1038/s41598-017-02693-1. 10.1038/s41598-017-02693-1 [pii] PubMed 28550309 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pPSU1 10000 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION High copy number plasmid 1 to prepare Penn State DNA molecular
weight ladders.
ACCESSION .
VERSION .
KEYWORDS pPSU1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10000)
AUTHORS Henrici RC, Pecen TJ, Johnston JL, Tan S
TITLE The pPSU Plasmids for Generating DNA Molecular Weight Markers.
JOURNAL Sci Rep. 2017 May 26;7(1):2438. doi: 10.1038/s41598-017-02693-1.
PUBMED 28550309
REFERENCE 2 (bases 1 to 10000)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10000
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 112..131
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 365..382
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 499..520
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 535..565
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 573..589
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 578..600
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 597..613
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 597..613
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7997..8014)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(7997..8013)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8006..8028)
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind complement(8222..8241)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 8341..8363
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(8401..8419)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 8487..8591
/gene="bla"
/label=AmpR promoter
CDS 8592..9452
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(8810..8829)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 9623..211
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
61 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
121 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
181 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
241 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
301 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
361 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
421 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
481 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
541 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
601 aaacagctat gaccatgatt acgccaagct tgatatcatc aaagccatga acaaagcagc
661 cgcgctggat gaactgatac cggggttgct gagtgaatat atcgaacagt caggttaaca
721 ggctgcggca ttttgtccgc gccgggcttc gctcactgtt caggccggag ccacagaccg
781 ccgttgaatg ggcggatgct aattactatc tcccgaaaga atccgcatac caggaagggc
841 gctgggaaac actgcccttt cagcgggcca tcatgaatgc gatgggcagc gactacatcc
901 gtgaggtgaa tgtggtgaag tctgcccgtg tcggttattc caaaatgctg ctgggtgttt
961 atgcctactt tatagagcat aagcagcgca acacccttat ctggttgccg acggatggtg
1021 atgccgagaa ctttatgaaa acccacgttg agccgactat tcgtgatatt ccgtcgctgc
1081 tggcgctggc cccgtggtat ggcaaaaagc accgggataa cacgctcacc agatatcgaa
1141 gcctacgcgc tgaacgccag cggtgtggtg aatatcatcg tgttcgatcc gaaaggctgg
1201 gcgctgttcc gttccttcaa agccgtcaag gagaagctgg atacccgtcg tggctctaat
1261 tccgagctgg agacagcggt gaaagacctg ggcaaagcgg tgtcctataa ggggatgtat
1321 ggcgatgtgg ccatcgtcgt gtattccgga cagtacgtgg aaaacggcgt caaaaagaac
1381 ttcctgccgg acaacacgat ggtgctgggg aacactcagg cacgcggtct gcgcacctat
1441 ggctgcattc aggatgcgga cgcacagcgc gaaggcatta acgcctctgc ccgttacccg
1501 aaaaactggg tgaccaccgg cgatccggcg cgtgagttca ccatgattca gtcagcaccg
1561 ctgatgctgc tggctgaccc tgatgagttc gtgtccgtac aactggcgta atcatggccc
1621 ttcggggcca ttgtttctct gtggaggagt ccatgacgaa agatgaactg attgcccgtc
1681 tccgctcgct gggtgaacaa ctgaaccgtg atgtcagcct gacggggacg aaagaagaac
1741 tggcgctccg tgtggcagag ctgaaagagg agcttgatga cacggatgaa actgccggtc
1801 aggacacccc tctcagccgg gaaaatgtgc tgaccggaca tgaaaatgag gtgggatcag
1861 cgcagccgga taccgtgatt ctggatacgt ctgaactggt cacggtcgtg gcactggtga
1921 agctgcatac tgatgcactt cacgccacgc gggatgaacc tgtggcattt gtgctgccgg
1981 gaacggcgtt tcgtgtctct gccggtgtgg cagccgaaat gacagagcgc ggcctggcca
2041 gaatgcaata acgggaggcg ctgtggctga tttcgataac ctgtctgcag ctgccattgc
2101 ccgcgccgat gaaacgatac gcgggtacat ggatatcggt acccttcatt tcgcataaat
2161 caccaactcg ttgcccggta acaacagcca gttccattgc aagtctgagc caacatggtg
2221 atgattctgc tgcttgataa attttcaggt attcgtcagc cgtaagtctt gatctcctta
2281 cctctgattt tgctgcgcga gtggcagcga catggtttgt tgttatatgg ccttcagcta
2341 ttgcctctcg gaatgcatcg ctcagtgttg atctgattaa cttggctgac gccgccttgc
2401 cctcgtctat gtatccattg agcattgccg caatttcttt tgtggtgatg tcttcaagtg
2461 gagcatcagg cagacccctc cttattgctt taattttgct catgtaattt atgagtgtct
2521 tctgcttgat tcctctgctg gccaggattt tttcgtagcg atcaagccat gaatgtaacg
2581 taacctgcag atcactgttg attctcgctg tcagaggctt gtgtttgtgt cctgaaaata
2641 actcaatgtt ggcctgtata gcttcagtga ttgcgattcg cctgtctctg cctaatccaa
2701 actctttacc cgtccttggg tccctgtagc agtaatatcc attgtttctt atataaaggt
2761 tagggggtaa atcccggcgc tcatgacttc gccttcttcc catttctgat cctcttcaaa
2821 aggccacctg ttactggtcg atttaagtca acctttaccg ctgattcgtg gaacagatac
2881 tctcttccat ccttaaccgg aggtgggaat atcctgcatt cccgaaccca tcgacgaact
2941 gtttcaaggc ttcttggacg tcgctggcgt gcgttccact cctgaagtgt caagtacatc
3001 gcaaagtctc cgcaattaca cgcaagaaaa aaccgccatc aggcggcttg gtgttctttc
3061 agttcttcaa ttcgaatatt ggttacgtct gcatgtgcta tctgcgccca tatcatccag
3121 tggtcgtagc agtcgttgat gttctccgct tcgataactc tgttgaatgg ctctccattc
3181 cattctcctg tgactcggaa gtgcatttat catctccata aaacaaaacc cgccgtagcg
3241 agttcagata aaataaatcc ccgcgagtgc gaggattgtt atgtaatatt gggtttaatc
3301 atctatatgt tttgtacaga gagggcaagt atcgtttcca ccgtactcgt gataataatt
3361 ttgcacggta tcagtcattt ctcgcacatt gcagaatggg gatttgtctt cattagactt
3421 ataaaccttc atggaatatt tgtatgccga ctctatatct ataccttcat ctacataaac
3481 accttcgtga tgtctgcatg gagacaagac accggatctg cacaacattg ataacgccca
3541 atctttttgc tcagactcta actcattgat actcatttat aaactccttg caatgtatgt
3601 cgtttcagct aaacggtatc agcaatgttt agatatctct agacttccga ttagaaacgt
3661 caaggcagca atcaggattg caatcatggt tcctgcatat gatgacaatg tcgccccaag
3721 accatctcta tgagctgaaa aagaaacacc aggaatgtag tggcggaaaa ggagatagca
3781 aatgcttacg ataacgtaag gaattattac tatgtaaaca ccaggcatga ttctgttccg
3841 cataattact cctgctaatt aatccttaac tttgcccacc tgccttttaa aacattccag
3901 tatatcactt ttcattcttg cgtagcaata tgccatctct tcagctatct cagcattggt
3961 gaccttgttc agaggcgctg agagatggcc tttttctgat agataatgtt ctgttaaaat
4021 atctccggcc tcatcttttg cccgcaggct aatgtctgaa aattgaggtg acgggttaaa
4081 aataatatcc ttggcaacct tttttatatc ccttttaaat tttggcttaa tgactatatc
4141 caatgagtca aaaagctccc cttcaatatc tgttgcccct aagaccttta atatatcgcc
4201 aaatacaggt agcttggctt ctaccttcac cgttgttcgg ccgatgaaat gcatatgcat
4261 aacatcgtct ttggtggttc ccctcatcag tggctctatc tgaacgcgct ctccactgct
4321 taatgacatt cctttcccga ttaaaaaatc tgtcagatcg gatgtggtcg gcccgaaaac
4381 agttctggca aaaccaatgg tgtcgccttc aacaaacaaa aaagatggga atcccaatga
4441 ttcgtcatct gcgaggctgt tcttaatatc ttcaactgaa gctttagagc gatttatctt
4501 ctgaaccaga ctcttgtcat ttgttttggt aaagagaaaa gtttttccat cgatactgat
4561 gtatcatgtc caaagaacga ttatctgcag atcgaacaac gagaatcaag atcactgagc
4621 taaatcccca cctgatgtgt gtgctttgtg gagggtactt cattgatgcc acaaccataa
4681 tagaatgtct acattccttc tgtaaaacgt gtattgttcg ttacctggag accagcaagt
4741 attgtcctat ttgtgatgtc caagttcaca agaccagacc actactgaat ataaggtcag
4801 ataaaactct ccaagatatt gtatacaaat tagttccagg gcttttcaaa aatgaaatga
4861 agagaagaag ggatttttat gcagctcatc cttctgctga tgctgccaat ggctctaatg
4921 aagatagagg agaggttgca gatgaagata agagaattat aactgatgat gagataataa
4981 gcttatccat tgaattcttt gaccagaaca gattggatcg gaaagtaaac aaagacaaag
5041 agaaatctaa ggaggaggtg aatgataaaa gatacttacg atgcccagca gcaatgactg
5101 tgatgcactt aagaaagttt ctcagaagta aaatggacat acctaatact ttccagattg
5161 atgtcatgta tgaggaggaa cctttaaagg attattatac actaatggat attgcctaca
5221 tttatacctg gagaaggaat ggtccacttc cattgaaata cagagttcga cctacttgta
5281 aaagaatgaa gatcagtcac cagagagatg gactgacaaa tgctggagaa ctggaaagtg
5341 actctgggag tgacaaggcc aacagcccag caggaggtat tccctccacc tcttcttgtt
5401 tgcctagccc cagtactcca gtgcagtctc ctcatccaca gtttcctcac atttccagta
5461 ctatgaatgg aaccagcaac agccccagcg gtaaccacca atcttctttt gccaatagac
5521 ctcgaaaatc atcagtaaat gggtcatcag caacttcttc tggttaatgt acataatcag
5581 atctctgcag ttgatgcgtg gatggagtcc cggatttatc cggtgatgag cgatatcccg
5641 gcactgtcag atttgatcac cagtatggtg gccagcggct atgactaccg gcgcgacgat
5701 gatgcgggct tgtggagttc agccgatctg acttatgtca ttacctatga aatgtgagga
5761 cgctatgcct gtaccaaatc ctacaatgcc ggtgaaaggt gccgggacca ccctgtgggt
5821 ttataagggg agcggtgacc cttacgcgaa tccgctttca gacgttgact ggtcgcgtct
5881 ggcaaaagtt aaagacctga cgcccggcga actgaccgct gagtcctatg acgacagcta
5941 tctcgatgat gaagatgcag actggactgc gaccgggcag gggcagaaat ctgccggaga
6001 taccagcttc acgctggcgt ggatgcccgg agagcagggg cagcaggcgc tgctggcgtg
6061 gtttaatgaa ggcgataccc gtgcctataa aatccgcttc ccgaacggca cggtcgatgt
6121 gttccgtggc tgggtcagca gtatcggtaa ggcggtgacg gcgaaggaag tgatcacccg
6181 cacggtgaaa gtcaccaatg tgggacgtcc gtcgatggca gaagatcgca gcacggtaac
6241 agcggcaacc ggcatgaccg tgacgcctgc cagcacctgg atctctgcag ccatggagat
6301 catctgccgg gacgtggctc ggggctatga gaacgtgccc attccctgtg tcaacggtgt
6361 ggatggggag ccctgccctg aggattacaa gtacatctca gagaactgcg agacgtccac
6421 catgaacatc gatcgcaaca tcacccacta acagcactgc acgtgtgtgg acgactgctc
6481 tagctccaac tgcctgtgcg gccagctcag catccggtgc tggtatgaca aggatgggcg
6541 attgctccag gaatttaaca agattgagcc tccgctgatt ttcgagtgta accaggcgtg
6601 ctcatgctgg agaaactgca agaaccgggt cgtacagagt ggcatcaagg tgcggctaca
6661 gctctaccga acagccaaga tgggctgggg ggtccgcgcc ctgtaaacca tcccacaggg
6721 gaccttcatc tgcgagtatg tcggggagct gatctctgat gctgaggctg atgtgagaga
6781 ggatgattct tacctcttcg acttagacaa caaggatgga gaggtgtact gcatagatgc
6841 ccgttactat ggcaacatca gccgcttcat caaccacctg tgtgacccca acatcattcc
6901 cgtccgggtc ttcatgctgc accaagacct gcgatttcca cgcatcgcct tcttcagttc
6961 ccgagacatc cggactgggg aggagctagg gtttgactat ggcgaccgct tctgggacat
7021 caaaagcaaa tatttcacct gccaatgtgg ctctgagaag tgcaagcact cagggcccgg
7081 atctctgcag gtgcagagtg atttgccgtg gtggctggtc tgccggggga cgattcataa
7141 gttccgctgt gtgccgcatc tcaccgggcg gcgctttgag cacggtgtga cggactgtta
7201 cacactgttc cgggatgctt atcatctggc ggggattgag atgccggact ttcatcgtga
7261 ggatgactgg tggcgtaacg gccagaatct ctatctggat aatctggagg cgacggggct
7321 gtatcaggtg ccgttgtcag cggcacagcc gggcgatgtg ctgctgtgct gttttggttc
7381 atcagtgccg aatcacgccg caatttactg cggcgacggc gagctgctgc accatattcc
7441 tgaacaactg agcaaacgag agaggtacac cgacaaatgg cagcgacgca cacactccct
7501 ctggcgtcac cgggcatggc gcgcatctgc ctttacgggg atttacaacg atttggtcgc
7561 cgcatcgacc ttcgtgtgaa aacgggggct gaagccatcc gggcactggc cacacagctc
7621 ccggcgtttc gtcagaaact gagcgacggc tggtatcagg tacggattgc cgggcgggac
7681 gtcagcacgt ccgggttaac ggcgcagtta catgagactc tgcctgatgg cgctgtaatt
7741 catattgttc ccagagtcgc cggggccaag tcaggtggcg tattccagat tgtcctgggg
7801 gctgccgcca ttgccggatc attctttacc gccggagcca cccttgcagc atggggggca
7861 gccattgggg ccggtggtat gaccggcatc ctgttttctc tcggtgccag tatggtgctc
7921 ggtggtgtgg cgcagatgct ggcaccgaaa gccagaactc cccgtataca gacaacggat
7981 aacgctgcag gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
8041 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
8101 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga
8161 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
8221 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
8281 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
8341 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
8401 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
8461 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
8521 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
8581 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
8641 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
8701 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
8761 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
8821 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
8881 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
8941 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
9001 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
9061 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
9121 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
9181 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
9241 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
9301 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
9361 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
9421 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
9481 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
9541 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
9601 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
9661 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
9721 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
9781 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
9841 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
9901 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
9961 agcccagctt ggagcgaacg acctacaccg aactgagata
//
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