出品公司: | Promega |
---|---|
载体名称: | pkk232-8 |
质粒类型: | 大肠杆菌表达载体 |
高拷贝/低拷贝: | -- |
启动子: | -- |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 5094 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | Ampicillin |
筛选标记: | -- |
备注: | -- |
产品目录号: | -- |
稳定性: | -- |
组成型: | -- |
病毒/非病毒: | 非病毒 |
买家导航
pkk232-8载体质粒基本信息
pkk232-8质粒图谱载体图谱和pkk232-8载体序列质粒序列多克隆位点信息
pkk232-8质粒载体简介
pkk232-8质粒序列载体序列
LOCUS pKK232-8 5094 bp DNA SYN DEFINITION pKK232-8 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..5094 /organism="pKK232-8" /mol_type="other DNA" terminator 15..58 /label="rrnB_T1_terminator" gene 269..928 /label="CAT/CamR" /gene="CAT/CamR" CDS 269..928 /label="ORF frame 2" /translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" misc_feature complement(1193..1210) /label="pBAD_rev_primer" /translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" misc_feature complement(1193..1210) /label="pTrcHis_rev_primer" /translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" terminator 1243..1400 /label="rrnB_terminator" /translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" terminator 1366..1409 /label="rrnB_T1_terminator" /translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" terminator 1541..1568 /label="rrnB_T2_terminator" /translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" misc_feature complement(1691..1708) /label="pTrcHis_rev_primer" /translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" misc_feature complement(1691..1708) /label="pBAD_rev_primer" /translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" terminator 1741..1898 /label="rrnB_terminator" /translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" terminator 1864..1907 /label="rrnB_T1_terminator" /translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" terminator 2039..2066 /label="rrnB_T2_terminator" /translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" misc_feature 2648..2839 /label="ROP" /translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" misc_feature complement(2833..2855) /label="pGEX_3_primer" /translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" rep_origin complement(3252..3871) /label="pBR322_origin" /translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" gene complement(4026..4886) /label="Ampicillin" /gene="Ampicillin" /translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" CDS complement(4026..4886) /label="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" promoter complement(4928..4956) /label="AmpR_promoter" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" ORIGIN 1 ttcccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 61 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg 121 ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact gccagggaat 181 tcccggggat ccgtcgacct gcagccaagc ttgagtagga caaatccgcc gagcttcgac 241 gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt 301 tgatatatcc caatcgcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg 361 tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa 421 taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc 481 ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg 541 ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga 601 cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct 661 ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt 721 gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt 781 caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt 841 tcatcatgcc gtctgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta 901 ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc agttattggt gcccttaaac 961 gcctggtgct acgcctgaat aagtgataat aagcggatga atggcagaaa ttcgtcgagg 1021 cggcacctcg ctaacggatt caccactcca agaattggag ccaatcaatt cttgcggaga 1081 actgtgaatg cgcaaaccaa cccttggcag aacatatcca tcgcgtccgc catctccagc 1141 agccgcacgc ggcgcatctc ggctgttttg gcggatgaga gaagattttc agcctgatac 1201 agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg 1261 cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta 1321 gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct 1381 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt 1441 aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg 1501 gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat 1561 ggcctttttg cgtttctaca aactcttcct gtcgtcatat ctacaagcca tccccccaca 1621 gatacggtaa actagcctcg tttttgcatc aggaaagcag ctgttttggc ggatgagaga 1681 agattttcag cctgatacag attaaatcag aacgcagaag cggtctgata aaacagaatt 1741 tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac 1801 gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat 1861 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 1921 gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa 1981 cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca aattaagcag 2041 aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctcttcctgt cgtcatatct 2101 acaagccatc cccccacaga tacggtaaac tagcctcgtt tttgcatcag gaaagcagtc 2161 gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg 2221 gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac gcgagcgaac 2281 gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa tggtcttcgg 2341 tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg 2401 gatctgcatc gcaggatgct gctggctacc ctgtggaaca cctacatctg tattaacgaa 2461 gcgctggcat tgaccctgag tgatttttct ctggtcccgc cgcatccata ccgccagttg 2521 tttaccctca caacgttcca gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag 2581 catcctctct cgtttcatcg gtatcattac ccccatgaac agaaatcccc cttacacgga 2641 ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca 2701 gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg cagacatctg 2761 tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga 2821 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc 2881 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg 2941 cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca 3001 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta 3061 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 3121 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 3181 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 3241 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 3301 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 3361 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 3421 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat 3481 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 3541 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 3601 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 3661 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 3721 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 3781 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 3841 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 3901 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 3961 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 4021 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 4081 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 4141 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 4201 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 4261 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 4321 cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct 4381 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 4441 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 4501 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 4561 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 4621 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa 4681 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 4741 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 4801 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 4861 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 4921 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 4981 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc 5041 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaa //