| 出品公司: | EMD Biosciences(Novagen) |
|---|---|
| 别名: | pET19b, pet 19b |
| 质粒类型: | 大肠杆菌蛋白表达 |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5717bp |
| 5' 测序引物: | T7 |
| 5' 测序引物序列: | 5'-TAATACGACTCACTATAGGG-3' |
| 载体标签: | N-His, N-EK 蛋白酶切位点 |
| 载体抗性: | Ampicillin |
| 备注: |
Hosts: E.coli. Related vectors: pBR322.
|
| 产品目录号: | 69677-3 |
| 稳定性: | 瞬时表达 Transient |
| 组成型: | 组成型 Constitutive |
| 病毒/非病毒: | 非病毒 |
| 表达宿主菌: | BL21(DE3) 、BLR(DE3)、Rosetta(DE3)、OrigamiB(DE3) |
| pET表达手册： |
pET表达手册英文版 pET表达手册中文版 |
| pET载体汇总: |
pET-3a pET-11a pET-12a pET-14b pET-15b pET-16b pET-17b pET-19b pET-20b pET-21a pET-22b pET-23a pET-24a pET-25b pET-26b pET-27b pET-28a pET-29a pET-30a pET-31b pET-32a pET-33b pET-34b pET-35b pET-37b pET-39b pET-40b pET-41a pET-42a pET-43.1a pET-44a pET-45b pET-47b pET-48b pET-49b pET-50b pET-51b pET-52b pETDuet-1 pACYCDuet-1 pCDFDuet-1 pRSFDuet-1 pColaDuet-1 |
买家导航
pET19b载体基本信息
pET19b载体图谱质粒图谱和多克隆位点信息
| Feature Name | Start | End | |
|---|---|---|---|
| T7_terminator | 316 | 188 | |
| T7_Terminal_primer | 256 | 274 | |
| EK | 347 | 336 | |
| T7_transl_en_RBS | 425 | 409 | |
| lacO | 470 | 443 | |
| T7_promoter | 488 | 470 | |
| pBRrevBam_primer | 591 | 572 | |
| lacI | 866 | 1957 | |
| tet (576 - 1190) | 2016 | 2630 | |
| ROP | 3269 | 3460 | |
| pGEX_3_primer | 3476 | 3454 | |
| Ampicillin | 5509 | 4649 | |
| AmpR_promoter | 5579 | 5551 |
| ORF | Start | End | |
|---|---|---|---|
| ORF frame 1 | 625 | 1125 | |
| ORF frame 2 | 998 | 1957 | |
| ORF frame 1 | 2435 | 1740 | |
| ORF frame 3 | 2013 | 2630 | |
| ORF frame 2 | 5509 | 4649 |
| Enzyme Name | Cut |
|---|---|
| ClaI | 24 |
| HindIII | 29 |
| BamHI | 319 |
| XhoI | 324 |
| NdeI | 331 |
| NcoI | 398 |
| XbaI | 437 |
| BglII | 503 |
| BclI | 1239 |
| ApaI | 1436 |
| HpaI | 1731 |
| EagI | 2293 |
| NruI | 2328 |
| MscI | 2800 |
| PstI | 4967 |
| AatII | 5644 |
| EcoRI | 5715 |
pET19b载体简介
pET-19b载体具有N端His标签和EK蛋白酶切位点。独特的酶切位点在载体图谱中被标注出来。需要注意的是，这些载体序列是依据载体pBR322的方向来进行编号的，所以T7标签表达区域在载体图谱上是反向的。
pET19b载体序列
LOCUS pET-19b 5717 bp DNA circular SYN
DEFINITION pET-19b
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank|
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTOAUTHORAD1|Novagen Inc.|
FEATURES Location/Qualifiers
source 1..5717
/organism="pET-19b"
/mol_type="other DNA"
terminator complement(188..316)
/label="T7_terminator"
misc_feature 256..274
/label="T7_Terminal_primer"
misc_feature complement(336..347)
/label="EK"
misc_feature complement(409..425)
/label="T7_transl_en_RBS"
misc_feature complement(443..470)
/label="lacO"
promoter complement(470..488)
/label="T7_promoter"
misc_feature complement(572..591)
/label="pBRrevBam_primer"
CDS 625..1125
/label="ORF frame 1"
/translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD
RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV
ANCRGD*"
misc_feature 866..1957
/label="lacI"
/translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD
RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV
ANCRGD*"
CDS 998..1957
/label="ORF frame 2"
/translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
CDS complement(1740..2435)
/label="ORF frame 1"
/translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ
RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER
CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSRSRCLMSELTYINCVAL
TARFPVGKPVVPAALMNRPTRGERRFAYWAPGWFFFSPVRRATADCPSPPGPERVAAS
GPRWFAPAGENPV*"
CDS 2013..2630
/label="ORF frame 3"
/translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
gene 2016..2630
/label="tet (576 - 1190)"
/gene="tet (576 - 1190)"
/translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
misc_feature 3269..3460
/label="ROP"
/translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
misc_feature complement(3454..3476)
/label="pGEX_3_primer"
/translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
gene complement(4649..5509)
/label="Ampicillin"
/gene="Ampicillin"
/translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
CDS complement(4649..5509)
/label="ORF frame 2"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(5551..5579)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag
241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt
301 cgggctttgt tagcagccgg atcctcgagc atatgcttgt cgtcgtcgtc gatatggccg
361 ctgctgtgat gatgatgatg atgatgatga tgatggccca tggtatatct ccttcttaaa
421 gttaaacaaa attatttcta gaggggaatt gttatccgct cacaattccc ctatagtgag
481 tcgtattaat ttcgcgggat cgagatctcg atcctctacg ccggacgcat cgtggccggc
541 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
601 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
661 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg
721 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga
781 gagcgtcgag atcccggaca ccatcgaatg gcgcaaaacc tttcgcggta tggcatgata
841 gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt
901 cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca
961 cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc
1021 caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc
1081 cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca
1141 actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc
1201 ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga
1261 tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga
1321 tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact
1381 gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt
1441 aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca
1501 aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac
1561 catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat
1621 ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc
1681 ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat
1741 caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca
1801 gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac
1861 cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
1921 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt
1981 agctcactca ttaggcaccg ggatctcgac cgatgccctt gagagccttc aacccagtca
2041 gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta
2101 tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct
2161 ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc
2221 tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta
2281 tcgccggcat ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct
2341 ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg cccgcgttgc
2401 aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa ggatcgctcg
2461 cggctcttac cagcctaact tcgatcactg gaccgctgat cgtcacggcg atttatgccg
2521 cctcggcgag cacatggaac gggttggcat ggattgtagg cgccgcccta taccttgtct
2581 gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac ctcgacctga atggaagccg
2641 gcggcacctc gctaacggat tcaccactcc aagaattgga gccaatcaat tcttgcggag
2701 aactgtgaat gcgcaaacca acccttggca gaacatatcc atcgcgtccg ccatctccag
2761 cagccgcacg cggcgcatct cgggcagcgt tgggtcctgg ccacgggtgc gcatgatcgt
2821 gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc cttactggtt agcagaatga
2881 atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc
2941 aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc
3001 gccctgcacc attatgttcc ggatctgcat cgcaggatgc tgctggctac cctgtggaac
3061 acctacatct gtattaacga agcgctggca ttgaccctga gtgatttttc tctggtcccg
3121 ccgcatccat accgccagtt gtttaccctc acaacgttcc agtaaccggg catgttcatc
3181 atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc ggtatcatta cccccatgaa
3241 cagaaatccc ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg cccttaacat
3301 ggcccgcttt atcagaagcc agacattaac gcttctggag aaactcaacg agctggacgc
3361 ggatgaacag gcagacatct gtgaatcgct tcacgaccac gctgatgagc tttaccgcag
3421 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
3481 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
3541 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
3601 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatatgcggt
3661 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
3721 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
3781 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
3841 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
3901 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
3961 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
4021 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
4081 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
4141 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
4201 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
4261 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
4321 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
4381 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
4441 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
4501 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
4561 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
4621 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
4681 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
4741 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
4801 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
4861 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
4921 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt
4981 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
5041 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
5101 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
5161 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
5221 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg
5281 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
5341 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
5401 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
5461 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
5521 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
5581 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
5641 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
5701 cctttcgtct tcaagaa
//
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