质粒名称: | pAWP78-PVCpnf_FLAG-pvc2 |
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目录编号: | 198285 |
存储实验室: | Feng Zhang |
载体骨架基本信息 |
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载体名称: | pAWP78 |
完整质粒大小: | 25305bp |
载体抗性: | Kanamycin, 50 μg/mL |
生长温度: | 37 ℃ |
宿主菌: | DH5a |
拷贝数: | 低拷贝 |
插入基因信息 |
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插入基因名称: | PVCpnf_FLAG-pvc2 |
物种来源: | Photorhabdus asymbiotica |
克隆方法: | Gibson Cloning |
文献引用方法 |
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材料和方法部分: | pAWP78-PVCpnf_FLAG-pvc2 was a gift from Feng Zhang (Addgene plasmid # 198285 ; http://n2t.net/addgene:198285 ; RRID:Addgene_198285) |
参考文献部分: | Programmable protein delivery with a bacterial contractile injection system. Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29. 10.1038/s41586-023-05870-7 PubMed 36991127 |
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载体基本信息
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载体简介
载体序列
LOCUS pAWP78-PVCpnf_FLAG-pvc2 25350 bp ds-DNA circular SYN 27-MAR-2023 DEFINITION Contains an external epitope tag on the sheath protein; for immunofluorescence-based binding assays. ACCESSION . VERSION . KEYWORDS pAWP78-PVCpnf_FLAG-pvc2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 25350) AUTHORS Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F TITLE Programmable protein delivery with a bacterial contractile injection system JOURNAL Nature March 29, 2023 REFERENCE 2 (bases 1 to 25350) AUTHORS . TITLE Direct Submission JOURNAL Exported Mar 27, 2023 from SnapGene Server 1.1.58 http://www.biofeng.com FEATURES Location/Qualifiers source 1..25350 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 238..261 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS complement(19824..20639) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind 20547..20566 /label=Kan-R /note="Kanamycin resistance gene, reverse primer" primer_bind 20631..20650 /label=pENTR-R /note="pENTR vectors, reverse primer" CDS complement(20943..22091) /codon_start=1 /product="trans-acting replication protein that binds to and activates oriV" /label=trfA /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR" CDS complement(22363..22734) /codon_start=1 /gene="traJ" /product="oriT-recognizing protein" /label=traJ /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE KQDELGKVMMGVVRPRAEP" oriT 22767..22876 /note="incP origin of transfer" rep_origin 23029..23660 /label=oriV /note="incP origin of replication" rep_origin 23969..24557 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 24458..24477 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 gcttaatcag gtagagaaaa aggatattac catcagttta actaatgatg caggtaccga 61 attattaatg acctggaatg tttctaatgc ttttcccact tcattgactt caccttcatt 121 tgatgccacc agtaatgata ttgcagtaca ggaaattacg ctgatggcag atcgggtgat 181 tatgcaggct gtttgaagca ttgatattta atcatctcat ataagggaac ttttatggac 241 tacaaagacg acgatgacaa gggaacaacc gttaccagtt atcctggcgt ttatattgaa 301 gaattaaata gcctggcctt gtcagtttca aatagcgcca cagcggttcc tgtttttgct 361 gtggacgaac aaaaccaata tattagtgaa gataatgcaa tccgtattaa ttcgtggatg 421 gattatctta atctgattgg caattttaat aatgaagaca aattagatgt ttctgtgcgt 481 gcttattttg ccaatggagg tggatattgt tatctcgtca aaacaacgag tttagaaaaa 541 attattccaa ccttggatga tgtaacctta ttggttgctg cgggcgaaga tattaaaacg 601 acagtagatg ttttatgtca gccaggaaaa gggttattcg cagtctttga tggccctgaa 661 acagagttga ctatcaacgg tgcggaagag gcaaaacaag cctataccgc cacaccattc 721 gctgcggttt attatccttg gttgaaagcg gattgggcta acatagatat tccacccagt 781 gcagtgatgg cgggagttta tgcatcggtg gatttatccc gtggtgtatg gaaagcgcct 841 gccaatgttg cgttgaaagg gggcctggaa cctaaatttt tagtcacgga tgaattgcag 901 ggtgaatata acactggccg cgctatcaat atgattcgta atttcagtaa cacaggtact 961 acggtttggg gtgcaagaac cctggaagat aaagacaatt ggcgttatgt tccagtgcga 1021 cgcttgttta attctgtgga gcgggatatc aagcgtgcca tgagctttgc tatgttcgag 1081 cctaataatc agcctacttg ggagcgggta cgggcggcga ttagcaacta cctttatagc 1141 ctgtggcaac aggggggatt agctggcagc aaagaagaag acgcttattt tgtgcaaatt 1201 ggtaaaggta taacgatgac acaggagcag attgatgcag ggcaaatgat tgttaaagtc 1261 ggtttggctg ctgtacggcc tgcggaattt atcattctcc agtttacgca agatgtagaa 1321 cagcgttaat catatgatta tgaggagtta tcatgtctgc tattctgaaa gcgcctggcg 1381 tttatattga agaagacgct tccctagcgt tgtctgtcag taacagcgcg actgccgtgc 1441 ctgtttttat cggaaaattt actccgacag tggttgattc aatccaagtc tgtacccgta 1501 tcagcaactg gcttgaattc acttcctctt tttccctagc tccaacagtt gagattgttg 1561 tccaatctaa cactgaatct gaatctgaat ctgaaactta ccactatatt gagacaatca 1621 atttatctcc agctgtggaa gcattgcgac tctattttca aaatggcgga ggagcttgct 1681 atatctaccc attaaatgat gctgaagatg aattggttct ggcggccata ccagaagtca 1741 ttgaacagaa aggtgatatt actctgttgg tttgcccgga actcgatctg gattacaaaa 1801 ctaagatcta tggcgcagtg agctcactgt tgaatgataa caaagtgggc tatttcctga 1861 ttgcggatag caatgatgga gaatctgtgt caggagtatg gaatagtgct aaggccgccg 1921 cctattatcc ccagttggaa actaacctaa aattttccac gttgcctggg gataaggaca 1981 ttcgtatcag cggttatcag gatgatgatg aaacacataa accgaaaaac ttggatgagc 2041 tcaggacaat caacgaggcg ttggcacagg atattgatgc aagattgctc gaggagaaac 2101 aacgtgctgt catcattccg ccaagtgctg ccattgcggg catttattgc caaacggata 2161 atcgtcgcgg tgtttggaaa gcgccagcca acgttgcgct cacagggatc gggagtttgc 2221 ttgataaggt agacgatgaa cggcagggag agatgaatga caagggaatc aatgtcatcc 2281 gttcatttac cgaccgtggt tttatggtct ggggagcccg tacttgtgtg gacgctgcca 2341 acatcagctg gcgttatatt cctgttcgtc gcctgttcaa ttccgttgaa cgagatatcc 2401 gccaggcgct gcgcgctgtg ttgtttgaaa ctaatagtca gcctacctgg gtacgtgcta 2461 aggctgccgt tgatcaatat ctttataccc tttggcagaa aaatgcattg atgggtgctc 2521 gcccggaaga agcttatttt gtgcaaattg gtcaggatat caccatgtcc gaggctgata 2581 ttaaacaggg taagatgatc atgactgttg gtttggcagc agtgcggcca gctgagttca 2641 tcattctgca atttacgcag gatgttgttc agtaatctcc atgactaaac gccaggcact 2701 gtattgacag tgcctactct aaccatcttg gaggaggtga tgatgatgga gagactccaa 2761 ccgggtgtga ctttaacaga aagtataatc acgatgggtc agcaagagat acccagtgct 2821 gtgccggtgt ttattggtta caccgttcgt tatccggaac aatcggaagc atcagtccgt 2881 atcgacagtt tggccgagta taccagcctg tttggtgacg accatgtgat gatgtttgct 2941 gtcaggcact attttgataa tggcgggcaa caggcatttg ttttacccct gaaggacaat 3001 atgccatcag tggagatgac cacagctgaa gcggaaaatc tgatagccgc attgcgctct 3061 gctacggtta gcgaagccat tggtgggcat agtcagatta cactgatttt ggtaccggat 3121 atggctcggc ttaatgacag tgatattgat gactcctcaa cccaggtaag cctgtggtcc 3181 caaggctggg aggcgctgct gcaattgagt caggttaggc ccaacctctt tgtgctgtta 3241 gatgcgccgg ataatgttga acaggcgcag aagtgtatga caacgctatc gtcagattat 3301 cgtcaatggg gggcagcata ttggcctcgt ctggaaacta cctatcagaa agaaatatct 3361 ggcaaggaca atgaatctca gggaattttc caggggactg ttctgtcacc cacagccgcg 3421 gtcgcagcgg taattcaacg cacggataac gacgcgggtg tttggaaagc accggccaat 3481 attgccttat cccaggttat tcgacctgtt aaatcttatc ttcagggaag tgtactgttt 3541 aacagcagcg gcacttcgct caatgtgatc cgcagtttcc caggtaaggg catacgggta 3601 tggggatgcc gcactctgga aaacacggat aatacgcagt ggcgctatct gcaaacacgt 3661 cggctggttt cctatgtaac agcgcatttg acccaattgg ctcgcatgta tgtcttcgag 3721 ccaaataatg aacttacctg gatgaagtta aaaggacaaa gttacaactg gttacggcaa 3781 ttatggttgc agggtggctt gtatggttca caggaggatg aggcatttaa cattctgtta 3841 ggcgtaaacg agacgatgac tgaggatgat gttcgtgcag gaaaaatgat catgaaagtt 3901 gagttggctg tgttgtttcc tgccgaattt attgagatca gtttggtgtt taatacccaa 3961 acagaggcgc tgtcttaaga aggaaaaagt acgatgaacg attattacac acccgtggta 4021 tcccatcgtt ttatggcgag ttttattttt aaccgcattc ccgatccgct ggatattcgt 4081 tttcagcgta tctctggcct tagtcgggaa ctacaggtga ctcagtacag tgagggagga 4141 gaaaatgccc gtaataacta tttagctgag aaaatccaac acggtacgtt gactttggaa 4201 cggggcgtga tgacagtctc gccattgacc tggatgtttg atcgggtatt gagtggtgaa 4261 aaaatcgctt atgccgatgt ggtggtgatg ctactgaatg aaaattcact gccattgtcc 4321 agttggacgt tgagcaatgc gctgccggta cgctggcaaa ccagcgactt tgacgctaac 4381 agcaatgcca tattggtgaa tacccttgaa ttgcgttacc aggatatgcg ctggcttgga 4441 gtcaaaatat gacagtagaa atcagagagt tacttatcca ggcaaaggta gtgccatcaa 4501 cacgaccgac tgaatcagaa cggcaaaacc attctttgat acaggaaagt ctggatgagg 4561 cgacttgggt ggaaacgata aaacgcgaag tgttggccgc attacgcgat gaggaagggt 4621 ggcgtccatg agtctgattg aacgtggttt agctaagctg acaattaatg cttataagga 4681 tagggaaggg aagatacggg caggaacgtt gcaggccatg tataaccctg actccttgca 4741 actggattac caaacggatt atcagcaatc ccaagcgatt aatagcgaaa agcaaagtag 4801 catttatgta caggccaagc ccgcagggtt atcacttgaa ttaatttttg atgccacgat 4861 gccgggtaac aaaaccccca ttgaagagca gctcatgcag ctcaagcaac tgtgcagtgt 4921 ggatgcaacc agtaacgaga cgcgattcct gcaagttaaa tggggcaaaa tgcgttggga 4981 aagtcggggt tactttgctg gcagggccaa gagtttgtct gtgaattaca ctttgtttga 5041 tcgtgatgcg actcccttga gggtacgggt aatattggca ttagtggctg atgaaagtct 5101 ggtgttgcag gagactgaac aaaatctgca atctccggca aaaatcgcat tacgcataca 5161 ggatggggta tctctggctc tgatggcagc cagtacggca tcaacattgt caggcggtgt 5221 ggattatctg acgctggcct ggcaaaacgg tctggataat ctcaatgggt tcgttccggg 5281 tgaaatattg caggccacca ggggagacga atcatgagcc accaactgaa aattattgca 5341 gatggtaagg cactgtcact tttggccgcg gtagatgtgg acacctgtta tcgggttaac 5401 agtatacctt ctgcgacatt gaaactgagc gtaccggata ggccactctc ttctttcagt 5461 cagacggatg ttcagacaga actggcccac tgtcaggtag ggaaaaccct gcgtctggaa 5521 ttgattgatg gtagcaaaaa atgggtgctg tttaatggtc ttattacccg taaggctctg 5581 agaattaaga ataagcaatt attgctcact ctggttgtca agcatcggtt gcaactgatg 5641 gtggataccc agcattcaca gctgtttaaa gacaaaagcg aaaaagcgat cttaagcacg 5701 ctattgaatc agaccggaat caatgctcgc ttcggaaaga tagcggcgtt agatcaaaag 5761 catgaacaga tggtgcaatt tcgttgttca gactggcatt ttctgttgtg ccgactgtcg 5821 gcaaccggtg catggttgtt acctgccata gaagacgttc agtttgttca acctgatgct 5881 ctgaaatcaa actcagccta taccttgaag agcagggggg atgagaacaa agacatcgtt 5941 gtcaaggatg cttactggca gtttgacaat caaatcaacc ccgctttgct ggaagtcagt 6001 ggctgggata tcagtaagca gcaggtacaa tcaggcggtc gctacggaaa aatcgcgttg 6061 ggtaaggcgg cactctctcc tgatggattg gcatccctta ataaaacggg ttgggacatt 6121 tgttatagca gtccgttaac aacccaggaa agcggttatc tggcacaggg attattgctt 6181 aaccagcgca tttctggggt gacaggagaa tttttgctca aaggagatgg gcgttaccag 6241 ttgggagaca acattcagct gactggattt ggttcacagt tagatggtac ggcaagcatt 6301 actgaggttc gccaccgtct taatcggcga attgattggg aaaccacggt gagcattggt 6361 ttacaacatg aatatttgcc gatattacct gatgctcccg aactacatat tgcgacagta 6421 gcgaaatatc agcaggacag tgcggtgtta aaccgtatcc ccattattct gccggtactg 6481 aatcgtccca atgaattttt gtgggccaga ttggggaaac cttatgctag ccatgaaagc 6541 ggtttctgtt tttacccaga gccaggtgac gaagttatta ttggtttttt tgaaaatgat 6601 ccgcgttatc cagttatttt aggtgctatg cataatccga aaaataaggc cccttttgaa 6661 ccaacccaag ataataggga aaaagtattg atcgttaaaa aaggtgaagc gcaacaacaa 6721 ttagtcattg atggcaaaga gaaaatgatc cgaattaatg cgggtgaaaa tcaaataatg 6781 cttcagcaag ataaagacat ttctctgtca acgaaaaaag aattaacact gaaagcgcag 6841 acaatgaatg ccacgatgga taaatcattg gcaatgtccg ggaaaaacag tgttgaaatc 6901 aaaggcgcaa aaattaatct tacccaatga aaggtgacga tgaatggaaa atcaaatact 6961 gacacaactc tatggtcgtg gttgggcttt tcctccggtc ttttcccttg aaaagggggt 7021 agagatggct gaaggggcgg aagatgtgag acaaagtttg cagattctgt ttagtactga 7081 gccgggggaa cgtcttatgc gtgaaaatta tggctgcgga ttaaatgatt ttatgtttga 7141 aaatatccgc aatgaactta ttgctgaaat tgaatcccat atccatgaca acgtattacg 7201 atatgaaccc cgggctgata tgactgatat tcaggttcgt caatcccctg gcatggggaa 7261 tactttgcaa gtgcaggtca tgtatcgcct gagagggagt gatatcaatc aacaaatcca 7321 gggagtactt gcactgagtg aaggccgggt gacggaggta gtatgagtga agcgattgtg 7381 gtggatggtg acgtgttaca gtttgatccc aactttggca atcggcaggt gacggttccc 7441 agcccaggaa aaattagcgg cacaggacat gcgcaggtaa gtggaaaaaa agtgtgtatt 7501 ctgggggatg agaaacaggt cagggtttct gcaacctata ttacaacaac acatactacg 7561 ccgggaacag gaaccattac tatcagtgct ctggatgctg gccagcaggc ccttcagtgt 7621 accagtgggg cggctttaat tatcaagggg cagcaattta cggcgatgtt tacgcctgaa 7681 ttgccagcca tgaataatac agtgactccg ccacaaccgg atgttacgac accttcatca 7741 ggaaaaggac gttttatcac tcaacaaaat tttgctaccg taaattagag tattgactga 7801 attaaataga attaacgaag gtgtaaataa ttatttattt gctgacgaat cgctgtgaca 7861 aataaacaca ggtgatgtta tggaattaaa tgagttaact aacaaattgt caaatttggt 7921 gccaatgacc gattttaaat tagataatcg agccagtttg caattgctta aatatattga 7981 agcgtatacg aagataatac cctttaattc tggcgataaa tattggaatg actttttctt 8041 tatgtcagga aatacgccag agaaacttgc aaaattatat cagaaagaaa tagaacccaa 8101 tggggagtta ttacctcagc aggctttttt gttggcggtt ttgcgtttat tggaaacacc 8161 aatatcctta ttaaatgtat tacctgctgc tcatcgtgag ctctattatc gggagctttt 8221 aggcttgtct tcccatgcgg cacagcctga tcaggttgct ttatctatgg aactgaattc 8281 gacagtgatg gaacagctgc tccctgaagg aaccctgttt gaggctggtc aggatgaaca 8341 aggcaatgca ttgcaatatg ccctggatgc cagtttgctg gctaatcgtg gatatatcag 8401 tgacttgcgc tggttacgga atgacgggga aaagcaatgg gttacttctg ctccatggga 8461 tttacaggca caggtgtcac tgccgtctga tgggatacga ttatttggta agacaaatag 8521 tgatcagcag gtatttggtg gggtgttgat aacgtcatca cttctggcga tggaagcggg 8581 gataaggaag atcattgtta cttttgagca ggagatgaac acccaagaac tggtggcaca 8641 ggtcagcagt ggaaatcaat ggctaacatt gacgtctgag gtaaataaga aagaggtcac 8701 actgacactg tcagacaaag aaccggcaat cagtgcgcca gaggatctgg ataatctctt 8761 tttcacgcaa ccggtactca ggctacaggg aaaggatagt caggcactgc cggaggtgac 8821 gggtatcagc gtttcggaaa aggatgatac taaggatacc tcttttgaga tgtatcactt 8881 aacaccattt ggttatagca gtgatataga gccattggag gaaaatccag cgttatattt 8941 aggctttact gatgtaaagc cagggcaaac actggcgctg tattggaaat taaaatcccc 9001 gcagcaacca accgtttcct ggtattacct ggatcaacat aatcaatggg ctgaattgga 9061 ttcatgggtc agtgatggaa cccagaatct gtatcaggat ggtacttggc acgttgagtt 9121 gcctgtggat gcatccaatc aggcagagca gatgccagtt ggacgctatt ggttgcgggc 9181 agtggtggag gtacccgctc atgagggggc gttggggaag gctccttggc tatatggtct 9241 aatctataac gccatgacgg caaccttggt taatgtagat agcatcagtg acagccattt 9301 cttaacccct ttgcctgcca gcagcataca gcggcccgtt gaacccatca ttgtgttggc 9361 atcggtcaac cagccttggg catcatgggg tggacgtata cctgaatcct acagtgcctt 9421 ttttgaacgg atagctcaaa acctgtctca tcgaaaccgg tccttaacct ggggaaatat 9481 ggtgacatta ctcaaagagc gttatgtcag catctttgat gttaagtatc caggtaatga 9541 tgaactcacc agagtgccag cattggagca gcagcaacta acagtgattc cagcaaaccg 9601 gtacaacgat agcgatgatt ctctgcgtcc ggtactgaat cctgctcgtc tgcaagagat 9661 ggctgattgg ttgcagcaga aagactctcc ctgggcctct attgaggtca ggaatccaga 9721 atacttggat gtgaaaatcc attacgaggt gatttttaaa cctgatgtga acgaagattt 9781 tggctatcgc cagctacagc agcaactgtg tgaggtgtat atgccttgga gcatagatga 9841 gcagcggccc gttgtattga ataacagcat taattatttc cagttgttag ccactattca 9901 acagcaaccg ctggttgagc gagtcactcg tctgacacta catcgggctg attcttctga 9961 tgagagtgat ggtacagcat ctgtggaagc caaagataat gaagtgctta ttttagtctg 10021 ggaagaggac gataatctgc aataccgagg aaatgactat gagtaatcag gatgcactgt 10081 ttcatagcgt taaagacgat attcactttg ataccttgct ggaacaagct catcaggtga 10141 ttgaaaaaca ggctgaaaaa ctgtggagtg atacggcaga gcatgatccg ggtatcacat 10201 ttttgcaggg aatcagttac ggtgtgtcag atttggctta ccgacataca ttacccctga 10261 aagatttact gactccggcg ccggatgagc agcagcaaga gggaattttt cctgccgaat 10321 ttggcccgca taatacactg acttgtgggc cggtgacagc ggatgattat cgcaaggcat 10381 tgttagatct acacagcagc gacagcctgg atggtactca gcaggatgag ggggattttc 10441 tgttccggag tgtgcaactg gtgcgtgaac cggaaaaaca gcgttatacc tattggtatg 10501 atgcaaccaa gagggaatat agctttgtca acagtgaagg ggctaaagag tttaccttgc 10561 gggggaatta ctggttgtat ctggaaccaa cccgttggac tcagggtaat attgccgctg 10621 ctaccagaca actgacagaa tttttgacta aaaatcgcaa tattggtgaa tctgtcagca 10681 acattatctg gctacaaccg gttgatctgc cactgttgct ggatgttgaa ctggatgatg 10741 atgtaggtgc acaggatgtc cccggtattt ttgcggcggt gtatagcacc gcagagcagt 10801 atctgatgcc tggagcacag cgttaccgta cggaagtact gcaaaatgct gggatgagca 10861 atgatcaaat cttcgaaggt ccattattgg aacatggctg gataccagag ctgccggcag 10921 cccgtgatta tactcaaagg ctcactctca atcttagccg gttggtaaat agtctgcttg 10981 agattgaggg cattaaacat gtgaatcgtc ttcgtctgga tgatagcttc gataaaactg 11041 ctattgaacc cgttaagggg gatacctggt cgtggtcgat caaagagggc tattatccac 11101 gtctttgggg agaagaccca cttaaccaat tggcgcaaca aaatggcccg cttagggtga 11161 tagccaaagg agggattagc gtcagtgtga gtaaagagca aatccaggcc agtttaccca 11221 gtcaatcact gattcaaaat gagccggtaa tattggctta cggccagcac cgtgacgttg 11281 gcagctatta tcccgtcagt gatactttgc cgccttgcta tggactacaa cattctttgt 11341 ctgaaagtga acacttattg ccacttcatc aatttatgtt gccatttgaa caattattgg 11401 cctgtggttg tcaacagata gccatgctcc cgcggttact ggcttttcag cgcgaaggtt 11461 atgaggtttg gggtgatcag tggcccttta agtcaggctc agtgaatgat gacgcccatc 11521 aagattatgc ccctgcatta aaggatttgt taggacagat tgcgctggat agtgatcatg 11581 aattggatat tattaattac ttgctgggtt actttggcac acagcgggca ccgcgtacct 11641 ttacgacaca actcgatgat tttcgtgcgg tccaacaggg ttatctggcc cagcaaccga 11701 cattgactta ccaccgctcc aatattcgta tcgatcaggt atcgtcgcta caaaaacgta 11761 ttgctgctcg catggggctg ggcggtgagt tgtttaaacc tcaaccggat ctgagccaac 11821 tgccttttta tttgattgaa catcgagcgt tgctgccagt caaacccaat agtcagtttg 11881 ataaggaaca gaaaccagcc tcggtgacag aggagggggg cagccaaaca ggtcaacatt 11941 atgtggtcat tgaacagaag ggcattgatg gcaagctgac acaggggcaa gtgatcaatt 12001 taattctgta tgaaggagag cagggagaaa cccaatttac gatacgcggt cagatggtat 12061 tcaaaaccga gggggataag ttttggttgg atgtgaataa tagtgcgcaa ctggaatata 12121 atctggcgcg ggtaatgaca gcagccaagg cgagtaaact cttttggcaa aacagcccgg 12181 tatggatgga ggatatgggc tatcgtctgg cctatgctag tgaccaatcc tcattgcctg 12241 tgaatcaacg gcgcttgacc cgcacagtgc aaactccatt cccgccgatg gttgttgtag 12301 gtagcgaaat caccctgtta aagcaggtgg ggatagtcaa tttaaaaaaa gcggagtcag 12361 aaaaacttta tgcaaaagtt gttagctttg atcgcattga agggaccttg attattgagc 12421 gtttgggtaa ttccactctg gcttttccta cctcggaaga ggcgtggcgg tatagttggt 12481 atttttcggg ggagaaatat gaaaggactg accgcttttc atttgtgatt agcgtagtag 12541 tgaacagtga cttaattaaa ttgcccggtg ttgatcccta taaattggaa gaatgggtga 12601 aagaaacgat tcttaccgaa tttccagctc atatttctat gattatccat tggatggatc 12661 gggaagcctt tttaaatttc gccaatacct atcagcgttg gcaaaataat ggtacgccac 12721 tgggggatgc ggcttattcc attctagaaa gtttgacact tggtaaattg ccatctgcct 12781 taaaaggtgt tggcacaatg cgtattgcca catctagtca aagagaagaa gtggtgggta 12841 gtaatggtga tcaatggaat acagatggaa taacccagaa tgaattattc tatgttccta 12901 aagagagcta ggaaaaataa atatctgcca ctaatgatgt tgaattaaat atgttttctg 12961 gagttaatca tgaacgaaac tcgttataat gcaactgtac aagaacaaca aacattatct 13021 aatccaaaag ctgttggacc tgacatcgat aaattaaagg ataaatttaa agagggcagt 13081 attcccctgc aaaccgattt caatgagtta attgatattg ccgatattgg acgtaaagcc 13141 tgtggtcaag cgccacaaca aaatggccca ggagaaggat tgaaattggc tgatgacggt 13201 acgcttaatt taaaaatagg cactttttcc aataaagact tttctccatt aatattaaaa 13261 gatgatgttt tatctgtaga tcttggtagt ggtctgacta atgaaaccaa tggaatctgt 13321 gtcggtcagg gcgatggtat tacagttaac actagcaatg tagctgtaaa acaaggtaac 13381 ggaattagcg ttactagtag tggtggtgtt gccgttaaag ttagtgctaa taagggactt 13441 agcgttgata gtagtggtgt tgcagttaaa gttaatactg ataagggaat tagcgttgat 13501 ggtaatggtg ttgcagttaa agttaatact agtaaaggaa ttagcgttga taatacaggt 13561 gttgcagtta tagctaatgc tagtaaggga attagcgttg atggtagtgg tgttgcagtt 13621 atagctaata ctagtaaagg aattagcgtt gatggtagtg gtgttgcagt tatagctaat 13681 actagtaaag gaattagcgt tgataataca ggtgttgcag ttatagctaa tgctagtaag 13741 ggaattagcg ttgatggtag tggtgttgca gttatagcta atactagtaa aggaattagc 13801 gttgatggta gtggtgttgc agttatagct aatactagta aaggaattag cgttgatagt 13861 agtggtgttg cagttaaagt taaagctaat ggcggaatta aagtagatgc taatggtgtt 13921 gcaattgatc ctaataatgt actccccaag ggagtgattg taatgttctc tggcagtact 13981 gcaccaactg gttgggcgtt atgtgatggc aataatggta caccaaattt aatcgatcga 14041 tttattttag gtgggaaagg gactgatatt aatggagtga gtactaatac agcttcaggt 14101 actaaaaata gtaagttatt cgatttcagt tctgatgaag ctacattaac tattgatggt 14161 aaaacactgg ggagagcatt atcgttacag caaataccta atcatgcaca ctttagtgga 14221 ataattatgg atacagagaa agttaattat tatggaagta aaaaaatcac aacaaatgtg 14281 tggggtgtaa caacaggaga taatacttca gtacgatata tttataagtc atcaggtgta 14341 cttgactcta acaataatgt ctccaacagt accttaggcg gaaacagtct gcagacgcac 14401 gatcatgata ttaagataac gggcacagga aaacattctc acaaaaacaa agtaacagtc 14461 ccttattata ttctggcttt catcataaag ctttaatata tatgaaaaat tgaaaatata 14521 aattatccat taataataaa gaggatatta gcatgacttc ggagccaaat ctgttaaacc 14581 ggattacaat tactattgaa gctaataatc aacaagtagc tagaaaagta ttgcatggct 14641 ccttgcttaa tcaagctaat ataaataaat tatttaattc atactttaat gaatatgaaa 14701 ttaatagggg tgtttattta gaaacattaa tcctgaatct tggtacgata aatttccatg 14761 attttaattc attgtttcct actctcctaa aagctgcatt gaataaagaa ttcagtcaat 14821 atcagataaa caaccatagg gaagaaatgc tatttaatga gacaatatca aatcaagcta 14881 ctgataagtc ttacatattt ggcgataaca aattaattga tgcagagaat ttcattcact 14941 ttttatatca aaagcattcc acattaaatc tagtagaagc aatgggaaat aatggtattg 15001 aaaaattaac aaatcagtta acacaaatag aaaataaatt tgcgttatta ttggcaaaaa 15061 gttgtttgtc tgaggaaggc ttaaaacgac tcttggctat caaacaaccc gatttattaa 15121 tcgctatcaa tcgcagatta tctgaaagaa taaatagacc acaatatcag gagaagcttg 15181 tttcctgcgg acaactgata tttagtgctc tgggatatat acaacagtac aatatacagg 15241 aaattcctaa accggatgaa aaagttattg cacgcataac aactgaactt aataataatg 15301 gtttgcttaa tacaatacct attattacac tatttcgtca gagtgggatt aacgattcat 15361 cactaaatga ttggctaaag aaaatctggc aggtgagatc aatttcacag ttatgcagaa 15421 agtatctttc tgctaaggaa taccaatatc tgtcagaaca ttttgtttca aagagcgtcg 15481 ataaaaatag atatgatgaa gagcccgtaa atcagagcat attatcaagg ttgaataata 15541 attccattaa agaaggaaat aatcacagtc aactctgtac tctcagtaga ctatattctg 15601 aacccgttgt attacctgaa caaaccattc tacgtcaggt tagtaataca gtagatcaga 15661 gcatattatc aaggttgaat aatgcctcca ttaaagaagg aaataaccaa agtcaacttc 15721 gcactctcag tagactatat tctgagcccg ttgcattacc tgaacaaacc attccacgtc 15781 aggttagtaa tacaggtata ttaattctat ggccaatgct acctacacta tttaaccagc 15841 ttggtctact tgagaaaaag aaatttatcc atcgtcaggc ccagtttaat gccgttgatt 15901 ttcttgatta cctgatttgg ggaaccgaag atgtgaaagt ggaacgaagg gttttgaata 15961 atgttctatg tgggttaatg gctgatgaaa ttactgaacc aatgcctatt gaaccagaaa 16021 aacaatggat aataattcaa tggctggacg ctattatctc ccaactttct ggctggaaaa 16081 agttaagtcg taatgacgtc cgtcaattat ttctacaacg accaggagaa ttactgatca 16141 atgaacagga aattaaaatc acaatacagc aacaaccatt tgatgctctg ttaactgatt 16201 ggccgtggcc aatgaatatg gcttgtttta gctggttgag tcaaccatta accattacgt 16261 ggttataacc attgaccaca atgacttagt ctgagtaaaa aatatgaata tatcgcctgt 16321 tttttatgat tcattgaatc aggataacga ccgtgatcta tcgtttttat ttagcgaact 16381 ggaacgaata gatctcgctc ttcaacacca tttttattgt gtagaaagtc agcgaagtga 16441 gctcctggat gagtttctgc tcactgaggc ggaagtggtg accaggctgg ataagccact 16501 tggtaaacct cattggataa atgatgatta tctggcgata tcgcaaaagg gcaatgtaag 16561 cctaatggca gcgtccagat taatggatct gatcgaacgc tttgaactga ctgattttga 16621 gcgcgatgtt ttactattag gcttattgcc ccattttgat agccgctatt atcgactgtt 16681 ttcgctgatt caagggggac aacagggtcg attaccttct tttgcgctgg cattggaact 16741 gttttgccac tcggcgctgg agaaacaggt acagcaagcg agttttctgc accgggcacc 16801 tttgatgggt tgccagctat tatccatcga tactagtcaa aaaacgctgg cctggctcca 16861 gactcccttt attactgaca gcggggtata tcacttttta ctggggcatc actacattat 16921 gccggcttta gaacattgtg ctgagtggtt aacaccgaca gggattggct gttatcctga 16981 aggattaaaa caagtactgg gtaacgtatt gttatctgac aacgataata ttagaccgat 17041 tgtcttatta cggggaatgg ccggcagtgc cagagcttat accattacta atatgatggc 17101 ttcagaaggg aagcaaacac tgctggtaga tatatccaaa cttgctgata gcgatgaaaa 17161 aaacattatt cttcagataa agcatatttt gcgggaaacc cgcatgcatg gagcatgttt 17221 attattacgg aatttttgct tgttagtgga acagaataaa caactattgg actccctgtc 17281 agagttattg aatcaacctg aattaagaat tgtttgcctg attgagcctt attccccatt 17341 ggtatggctg aaaaagatac cggtattact gattgagatg ccacttttaa cgcctgcgga 17401 aaaagccaga ttgttaattg ccagcttacc ggataattgt tccgaggata ttgatacgat 17461 aactttaagc cagcgttaca cttttaaccc agaaaccctg ccattgattt tgcaagaggc 17521 ccagctttat caacagcagc gagatccgct ggatatcttg cagcaatgcg atatacgcca 17581 ggcattaaat ttgcgtgctc aacaaaattt cggtcaattg gcacagcgga ttattcctaa 17641 gcgctcatta aaggatttat tggtatccga tgagattgct cagcagttac gggaaatact 17701 catagcaatt aagtatcggg aacaggttct ggcgggaggg tttaaagata aaattgccta 17761 tggcactggt atcagcgccc tgttttatgg tgattcaggc actggaaaaa ccatggcagc 17821 agaagtgatt gctgaccaca ttggcgttga cttaataaaa gtggatttat ctacagtagt 17881 gaataaatac atcggtgaaa cagaaaaaaa cttatcccgt attttcgatt tggcggaaca 17941 ggatgcaggg gtattattct ttgatgaagc tgacgcactg tttggtaaac gcagtgaaac 18001 taaagattcc caggacagac atgccaatat tgaagtttct tacttattac agcgcctgga 18061 gaattacccg ggtctggtca ttttatccac caataatcgt ggtcatttag acagtgcttt 18121 taatcgtcgt tttactttca ttacccgttt tacttacccg gatgaaaaaa tccgtaaaaa 18181 aatgtggcag gaaatttggc ctagaaatat aaaaatatcg gaagatatcg attttaacga 18241 attagctcaa cgaacaagcg tgactggcgc gaatatccgc aatattgctt tattgtcttc 18301 attctttgct tcagagcagg ggaatgatga agtcagtaat gaaaatattg aaattgcatt 18361 gaagcgtgaa ttagctaaag tcggacgatt aacattttaa aagttatcac aatgaaagta 18421 ttgaaatatt aaataaattt attaccaaaa agttatcacg atataattta agagaggttt 18481 tttatgttaa acacgcaaac tattattgat gtcaataagg caatggatgc catgctgcgc 18541 gcatatctga atcaagatat tgccattcgt tttgatctac ctgaattgga tactatgcaa 18601 tctgatgcga tggtaagtat ctttctttat gacattcatg aagatttaca gcttcgctcg 18661 gcagaatcaa gagggtttga tgtttatgcc gggaggttat tgcctggttg ggtaaatatt 18721 aaatgtaact atctgattac ctattgggaa gcttctaagc cagcgactga tgccagcagt 18781 ccggatagcc aacctgataa ccaggcaata caagtgatgt cacaagtatt aaatgccttg 18841 attaataatc gtcaattggc aggtattcct ggtgcttata ctcaggttgt accgcctaaa 18901 gagagtttaa atagcctggg gaatttctgg caatcactgg gtaatcgccc acggctttct 18961 ctcaattatt cagtgacagt acctgttagc ctaaacgatg gtcaggatag cgcgactccg 19021 gttaccgcgg tttcttctac agtggaacaa acggcatcgc tcagtcaaga agtggttagt 19081 catgctttac gcgaattact cattacggaa ttaggaggag gagaggataa ccggttggta 19141 ctgagtaaag ttgaattatc cgcagtgaaa gagacgatga ctcaagacag tccggctcag 19201 atgattatat tgttgtctgt ttcaggcatt acacgacagg aatatttgaa ggaaattgat 19261 aatatctttg atcgttgggt aaataatgct gaagttatta ccactattga tgattgtggg 19321 attagaattg aaagtataac gaaagataat cttgtaggaa tttaaatttt tttaattaaa 19381 taaaggaatt aatattgatt tgatagagca tgtaatcaat aacttctatg aaaatgttga 19441 aaaacaatac acggaaacac ttaacttaca attaaattgt taacggagat gatgataaga 19501 atggtcagtc accagataaa atgatctctg gtcaatttta ggaaagctga aaaaacctga 19561 ccaaatgcgt cgtaaattct atgcttagaa agactcggtg ccagtgtaat acctaagata 19621 tggtttttat ttccatatct gttcgaaaac ttagaaataa caagttcaac aataaccagg 19681 gactttaaat atcggatcct tgtcgggaag atgcgtgatc tgatccttca actcagcaaa 19741 agttcgattt attcaacaaa gccgccgtcc cgtcaagtca gcgtaatgct ctgccagtgt 19801 tacaaccaat taaccaattc tgattagaaa aactcatcga gcatcaaatg aaactgcaat 19861 ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga 19921 gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg 19981 actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt 20041 gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagctt atgcatttct 20101 ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc 20161 aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa 20221 ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca 20281 atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt cccggggatc 20341 gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga 20401 ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg 20461 ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag 20521 attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca 20581 tccatgttgg aatttaatcg cggcctcgag caagacgttt cccgttgaat atggctcata 20641 acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt 20701 ttatcttgtg caatgtaaca tcagagattt tgagacacaa cgtggctttc gacttccggc 20761 aagctatacg cgccctagaa ttgtcaattt taatcctctg tttatcggca gttcgtagag 20821 cgcgccgtgc gtcccgagcg atactgagcg aagcaagtgc gtcgagcagt gcccgcttgt 20881 tcctgaaatg ccagtaaagc gctggctgct gaacccccag ccggaactga ccccacaagg 20941 ccctagcgtt tgcaatgcac caggtcatca ttgacccagg cgtgttccac caggccgctg 21001 cctcgcaact cttcgcaggc ttcgccgacc tgctcgcgcc acttcttcac gcgggtggaa 21061 tccgatccgc acatgaggcg gaaggtttcc agcttgagcg ggtacggctc ccggtgcgag 21121 ctgaaatagt cgaacatccg tcgggccgtc ggcgacagct tgcggtactt ctcccatatg 21181 aatttcgtgt agtggtcgcc agcaaacagc acgacgattt cctcgtcgat caggacctgg 21241 caacgggacg ttttcttgcc acggtccagg acgcggaagc ggtgcagcag cgacaccgat 21301 tccaggtgcc caacgcggtc ggacgtgaag cccatcgccg tcgcctgtag gcgcgacagg 21361 cattcctcgg ccttcgtgta ataccggcca ttgatcgacc agcccaggtc ctggcaaagc 21421 tcgtagaacg tgaaggtgat cggctcgccg ataggggtgc gcttcgcgta ctccaacacc 21481 tgctgccaca ccagttcgtc atcgtcggcc cgcagctcga cgccggtgta ggtgatcttc 21541 acgtccttgt tgacgtggaa aatgaccttg ttttgcagcg cctcgcgcgg gattttcttg 21601 ttgcgcgtgg tgaacagggc agagcgggcc gtgtcgtttg gcatcgctcg catcgtgtcc 21661 ggccacggcg caatatcgaa caaggaaagc tgcatttcct tgatctgctg cttcgtgtgt 21721 ttcagcaacg cggcctgctt ggcctcgctg acctgttttg ccaggtcctc gccggcggtt 21781 tttcgcttct tggtcgtcat agttcctcgc gtgtcgatgg tcatcgactt cgccaaacct 21841 gccgcctcct gttcgagacg acgcgaacgc tccacggcgg ccgatggcgc gggcagggca 21901 gggggagcca gttgcacgct gtcgcgctcg atcttggccg tagcttgctg gaccatcgag 21961 ccgacggact ggaaggtttc gcggggcgca cgcatgacgg tgcggcttgc gatggtttcg 22021 gcatcctcgg cggaaaaccc cgcgtcgatc agttcttgcc tgtatgcctt ccggtcaaac 22081 gtccgattca ttcaccctcc ttgcgggatt gccccgactc acgccggggc aatgtgccct 22141 tattcctgat ttgacccgcc tggtgccttg gtgtccagat aatccacctt atcggcaatg 22201 aagtcggtcc cgtagaccgt ctggccgtcc ttctcgtact tggtattccg aatcttgccc 22261 tgcacgaata ccagctccgc gaagtcgctc ttcttgatgg agcgcatggg gacgtgcttg 22321 gcaatcacgc gcaccccccg gccgttttag cggctaaaaa agtcatggct ctgccctcgg 22381 gcggaccacg cccatcatga ccttgccaag ctcgtcctgc ttctcttcga tcttcgccag 22441 cagggcgagg atcgtggcat caccgaaccg cgccgtgcgc gggtcgtcgg tgagccagag 22501 tttcagcagg ccgcccaggc ggcccaggtc gccattgatg cgggccagct cgcggacgtg 22561 ctcatagtcc acgacgcccg tgattttgta gccctggccg acggccagca ggtaggccta 22621 caggctcatg ccggccgccg ccgccttttc ctcaatcgct cttcgttcgt ctggaaggca 22681 gtacaccttg ataggtgggc tgcccttcct ggttggcttg gtttcatcag ccatccgctt 22741 gccctcatct gttacgccgg cggtagccgg ccagcctcgc agagcaggat tcccgttgag 22801 caccgccagg tgcgaataag ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac 22861 ttcacctatc ctgcccggct gacgccgttg gatacaccaa ggaaagtcta cacgaaccct 22921 ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat 22981 gaccccgaag cagggttatg cagcggaaaa gatccgtcga ccctttccga cgctcaccgg 23041 gctggttgcc ctcgccgctg ggctggcggc cgtctatggc cctgcaaacg cgccagaaac 23101 gccgtcgaag ccgtgtgcga gacaccgcgg ccgccggcgt tgtggatacc tcgcggaaaa 23161 cttggccctc actgacagat gaggggcgga cgttgacact tgaggggccg actcacccgg 23221 cgcggcgttg acagatgagg ggcaggctcg atttcggccg gcgacgtgga gctggccagc 23281 ctcgcaaatc ggcgaaaacg cctgatttta cgcgagtttc ccacagatga tgtggacaag 23341 cctggggata agtgccctgc ggtattgaca cttgaggggc gcgactactg acagatgagg 23401 ggcgcgatcc ttgacacttg aggggcagag tgctgacaga tgaggggcgc acctattgac 23461 atttgagggg ctgtccacag gcagaaaatc cagcatttgc aagggtttcc gcccgttttt 23521 cggccaccgc taacctgtct tttaacctgc ttttaaacca atatttataa accttgtttt 23581 taaccagggc tgcgccctgt gcgcgtgacc gcgcacgccg aaggggggtg cccccccttc 23641 tcgaaccctc ccggcccgct aacgcgggcc tcccatcccc ccaggggctg cgcccctcgg 23701 ccgcgaacgg cctcacccca aaaatggcag ccaagctgac ccgctaggga cgtgaagtcg 23761 attccttcga tggttagcaa tcaaagactc agagtgctgt ctggagcgtg aatctaacgg 23821 tacgtatctc gattgctcgg tcgctattcg cactctgcga aagttcgtac cgctcattca 23881 ctaggttgcg aatcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag 23941 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct 24001 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac 24061 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc 24121 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 24181 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 24241 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 24301 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 24361 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 24421 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 24481 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 24541 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 24601 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta 24661 ccgcctttga gtgagctgtc gcgaatgcat ctagatataa ttacatcttc atcattcggt 24721 attttaaatg acatatatcc tccttccgaa tagttttaat ggtgatgttc gattggacag 24781 tgttttccat tattaaaatt ttaagtatca catcatgttt aatgaaggat tgatgtttat 24841 tagaatattc ataatagctt tgtctttgcc tctatgtaaa tgaaattgca taaaaagcaa 24901 attgcattat tggcaatgta cttattctgg atttttcggc acgataaatt attggtgtaa 24961 gttttatatc atgagtaaaa attcggaatg atattcctgt cattttgtgg cggtagcgtt 25021 actcaagata ttatcctaca tcatatgttg attattgtaa tcaatcgagc tattttttgt 25081 ctaaaaaggg gcttttatgt ctacaagtac atctcaaatt gcggttgaat atcctattcc 25141 tgtctatcgc tttattgttt ctgtcggaga tgagaaaatt ccatttaata gtgtttcagg 25201 attagatatt agttatgaca ccattgaata ccgagatggt gttggtaatt ggttcaaaat 25261 gccgggtcag agtcagagca ctaatatcac cttgcgtaaa ggcgttttcc cggggaaaac 25321 agaactgttt gattggatta actctattca //
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