| 质粒名称: | mKO2-SLBP(18-126) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 83914 |
| 存储实验室: | Michael Lin |
|
载体骨架基本信息 |
|
| 载体名称: | pLL3.7m |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 6500 bp |
| 完整质粒大小: | 7426 bp |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | mKO2-SLBP(18-126) |
| 物种来源: | H. sapiens (human), Synthetic |
| 插入大小: | 1035 bp |
| 启动子 | CMV |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | NheI (未破坏) |
| 3' 克隆位点: | EcoRI (未破坏) |
| 5' 测序引物 | agctggtttagtgaaccgtcagatc |
| 3' 测序引物 | ggaaccggaacccttaaaca |
|
文献引用方法 |
|
| 材料和方法部分: | mKO2-SLBP(18-126) was a gift from Michael Lin (Addgene plasmid # 83914 ; http://n2t.net/addgene:83914 ; RRID:Addgene_83914) |
| 参考文献部分: | Fluorescent indicators for simultaneous reporting of all four cell cycle phases. Bajar BT, Lam AJ, Badiee RK, Oh YH, Chu J, Zhou XX, Kim N, Kim BB, Chung M, Yablonovitch AL, Cruz BF, Kulalert K, Tao JJ, Meyer T, Su XD, Lin MZ. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4045. 10.1038/nmeth.4045 PubMed 27798610 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS mKO2-SLBP(18-126) 7426 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Fluorescent probe for S/G2 cell cycle transition.
ACCESSION .
VERSION .
KEYWORDS mKO2-SLBP(18-126)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7426)
AUTHORS Bajar BT, Lam AJ, Badiee RK, Oh YH, Chu J, Zhou XX, Kim N, Kim BB,
Chung M, Yablonovitch AL, Cruz BF, Kulalert K, Tao JJ, Meyer T, Su
XD, Lin MZ
TITLE Fluorescent indicators for simultaneous reporting of all four cell
cycle phases.
JOURNAL Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4045.
PUBMED 27798610
REFERENCE 2 (bases 1 to 7426)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7426
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 63..366
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 368..566
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
LTR 584..764
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 811..936
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1435..1668
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2195..2312
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2371..2684
/label=U6 promoter
/note="RNA polymerase III promoter for mouse U6 snRNA (Das
et al., 1988)"
primer_bind 2603..2623
/label=mU6-F
/note="Mouse U6 promoter, forward primer"
primer_bind 2701..2717
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 2759..2792
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
enhancer 2908..3211
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 3212..3415
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
protein_bind 4513..4546
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
misc_feature 4602..5190
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4655..4675)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5073..5084)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(5193..5209)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
LTR 5432..5612
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
rep_origin complement(5674..6262)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5754..5773)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6433..7293)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7056..7075
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7294..7398)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
361 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
421 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
481 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
541 gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga
601 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
661 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
721 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
781 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
841 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
901 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
961 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
1021 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
1081 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
1141 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
1201 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
1261 accgcacagc aagcggccgg ccgcgctgat cttcagacct ggaggaggag atatgaggga
1321 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
1381 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
1441 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
1501 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
1561 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
1621 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
1681 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
1741 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
1801 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
1861 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
1921 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
1981 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
2041 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
2101 tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc
2161 aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
2221 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
2281 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
2341 cagtttggtt agtaccgggc ccgctctaga gatccgacgc cgccatctct aggcccgcgc
2401 cggccccctc gcacagactt gtgggagaag ctcggctact cccctgcccc ggttaatttg
2461 catataatat ttcctagtaa ctatagaggc ttaatgtgcg ataaaagaca gataatctgt
2521 tctttttaat actagctaca ttttacatga taggcttgga tttctataag agatacaaat
2581 actaaattat tattttaaaa aacagcacaa aaggaaactc accctaactg taaagtaatt
2641 gtgtgttttg agactataaa tatcccttgg agaaaagcct tgttaacgcg cggtgaccct
2701 cgaggtcgac ggtatcgata agctcgcttc acgagattcc agcaggtcga gggacctaat
2761 aacttcgtat agcatacatt atacgaagtt atattaaggg ttccaagctt aagcggccgc
2821 gtggataacc gtattaccgc catgcattag ttattaatag taatcaatta cggggtcatt
2881 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
2941 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
3001 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
3061 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
3121 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
3181 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
3241 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
3301 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
3361 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt
3421 agtgaaccgt cagatccgct agcaccaggt atggtgagcg tgatcaagcc cgagatgaag
3481 atgaggtact acatggacgg ctccgtcaat gggcatgagt tcacaatcga gggtgagggc
3541 acaggcagac cttacgaggg acatcaggag atgacactgc gcgtcacaat ggccgagggc
3601 gggccaatgc ctttcgcctt cgacctggtg tcccacgtgt tctgttacgg ccacagagtt
3661 tttaccaagt acccagaaga gatcccagac tatttcaagc aggcctttcc tgagggcctg
3721 tcctgggaga ggtccctgga gttcgaggac ggcggctccg cctccgtgag cgcccacatc
3781 agcctgaggg gcaacacctt ctaccacaag tccaagttca ccggcgtgaa cttccccgcc
3841 gacggcccca tcatgcagaa ccagagcgtg gactgggagc cctccaccga gaagatcacc
3901 gccagcgacg gcgtgctgaa gggcgacgtg accatgtacc tgaagctgga gggcggcggc
3961 aaccacaagt gccagatgaa gaccacctac aaggccgcca aggagatcct ggagatgccc
4021 ggcgaccact acatcggcca caggctggtg aggaagaccg agggcaacat caccgagcag
4081 gtggaggacg ccgtggccca ctactcaatg ttgccttccc tgggaggata cccatcacac
4141 tggcgaccac tcgaggccag cccgccgtcc cccgcgcgat ggagcctggg acggaagcgc
4201 agagccgacg gcaggcgctg gaggcccgaa gacgccgagg aggcagagca ccgcggcgcc
4261 gagcgcagac ccgagagctt taccactcct gaaggcccta aaccccgttc cagatgctct
4321 gactgggcaa gtgcagttga agaagatgaa atgaggacca gagttaacaa agaaatggca
4381 agatataaaa ggaaactcct catcaatgac tttggaagag agagaaaatc atcatcagga
4441 agttctgatt caaaggagtc tatgtctact gtgccggctg actaagttta aacgaattcg
4501 tcgagggacc taataacttc gtatagcata cattatacga agttatacat gtttaagggt
4561 tccggttcca ctaggtacaa ttcgatatca agcttatcga taatcaacct ctggattaca
4621 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
4681 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
4741 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
4801 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
4861 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
4921 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
4981 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
5041 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
5101 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
5161 gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga tcgagaccta
5221 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg
5281 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga
5341 ccaatgactt acaaggcagc tgacgcgtga ttttaaaaga aaagggggga ctggaagggc
5401 taattcactc ccactgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc
5461 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
5521 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
5581 acccttttag tcagtgtgga aaatctctag cagcatgtga gcaaaaggcc agcaaaaggc
5641 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
5701 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
5761 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
5821 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
5881 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
5941 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
6001 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
6061 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
6121 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
6181 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
6241 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
6301 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
6361 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
6421 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
6481 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
6541 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
6601 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
6661 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
6721 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
6781 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
6841 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
6901 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
6961 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
7021 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
7081 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
7141 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
7201 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
7261 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
7321 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
7381 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgac
//
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