pDC316-mCMV-tdTomato

价格:1500元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: --
载体名称: pDC316-mCMV-tdTomato
质粒类型: 腺病毒载体
高拷贝/低拷贝: --
克隆方法: --
启动子: CMV
载体大小: 6596 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: --
克隆菌株: --
宿主细胞(系): --
备注: --
产品目录号: --
稳定性: --
组成型/诱导型: --
病毒/非病毒: --

载体质粒图谱和多克隆位点信息

pDC316-mCMV-tdTomato载体图谱

载体简介

载体序列

LOCUS       pDC316-ZsGreen1-shRNA              5141 bp ds-DNA     circular SYN 26-OCT-2017
DEFINITION  synthetic circular DNA
KEYWORDS    pDC316-ZsGreen1-shRNA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   .
  TITLE     Direct Submission
            http://www.biofeng.com/
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    469..992
                     /label=MCMV promoter
     CDS             1003..1698
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     polyA_signal    1836..1957
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1975..2215
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     polyA_signal    2264..2398
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    2418..2451
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     rep_origin      complement(2875..3463)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3634..4494)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4495..4599)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    4682..453
                     /label=Ad
ORIGIN
        1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt
       61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg
      121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg
      181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt
      241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa
      301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg
      361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc
      421 gggtcaaagt tggcgtttta ttattatagt cagntctaga gatatactga gtcattaggg
      481 actttccaat gggttttgcc cagtacataa ggtcaatagg ggtgaatcaa caggaaagtc
      541 ccattggagc caagtacact gagtcaatag ggactttcca ttgggttttg cccagtacaa
      601 aaggtcaata gggggtgagt caatgggttt ttcccattat tggcacgtac ataaggtcaa
      661 taggggtgag tcattgggtt tttccagcca tttaattaaa acgccatgta ctttcccacc
      721 attgacgtca atgggctatt gaaactaatg caacgtgacc tttaaacggt actttcccat
      781 agctgattaa tgggaaagta ccgttctcga gccaatacac gtcaatggga agtgaaaggg
      841 cagccaaaac gtaacaccgc cccggttttc ccctggaaat tccatattgg cactcattct
      901 attggctgag ctgcgttcta cgtgggtata agaggcgcga ccagcgtcgg taccgtcgca
      961 gtcttcggtc tgaccaccgt agaacgcaga tcgaattcca ccatggccca gtccaagcac
     1021 ggcctgacca aggagatgac catgaagtac cgcatggagg gctgcgtgga cggccacaag
     1081 ttcgtgatca ccggcgaggg catcggctac cccttcaagg gcaagcaggc catcaacctg
     1141 tgcgtggtgg agggcggccc cttgcccttc gccgaggaca tcttgtccgc cgccttcatg
     1201 tacggcaacc gcgtgttcac cgagtacccc caggacatcg tcgactactt caagaactcc
     1261 tgccccgccg gctacacctg ggaccgctcc ttcctgttcg aggacggcgc cgtgtgcatc
     1321 tgcaacgccg acatcaccgt gagcgtggag gagaactgca tgtaccacga gtccaagttc
     1381 tacggcgtga acttccccgc cgacggcccc gtgatgaaga agatgaccga caactgggag
     1441 ccctcctgcg agaagatcat ccccgtgccc aagcagggca tcttgaaggg cgacgtgagc
     1501 atgtacctgc tgctgaagga cggtggccgc ttgcgctgcc agttcgacac cgtgtacaag
     1561 gccaagtccg tgccccgcaa gatgcccgac tggcacttca tccagcacaa gctgacccgc
     1621 gaggaccgca gcgacgccaa gaaccagaag tggcacctga ccgagcacgc catcgcctcc
     1681 ggctccgcct tgccctaaag atctcgagat ccaccggatc tagataactg atcataatca
     1741 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     1801 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     1861 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     1921 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg cgtctgggca ggaagagggc
     1981 ctatttccca tgattccttc atatttgcat atacgataca aggctgttag agagataatt
     2041 agaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa
     2101 taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt
     2161 accgtaactt gaaagtattt cgatttcttg ggtttatata tcttgtggaa aggacgaaac
     2221 acctgcagtt ttttggatcc caatgtcgac ctcgacttcg agcaacttgt ttattgcagc
     2281 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     2341 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcgt
     2401 ctagcatcga agatccaata acttcgtata gcatacatta tacgaagtta taagtagctt
     2461 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     2521 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     2581 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     2641 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     2701 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     2761 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     2821 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     2881 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     2941 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     3001 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     3061 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     3121 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     3181 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     3241 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     3301 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     3361 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     3421 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     3481 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     3541 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     3601 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     3661 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     3721 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     3781 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     3841 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     3901 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     3961 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     4021 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     4081 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     4141 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     4201 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     4261 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     4321 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     4381 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     4441 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     4501 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     4561 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     4621 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     4681 tctaggcaaa atagcaccct cccgctccag aacaacatac agcgcttcac agcggcagcc
     4741 taacagtcag ccttaccagt aaaaaagaaa acctattaaa aaaacaccac tcgacacggc
     4801 accagctcaa tcagtcacag tgtaaaaaag ggccaagtgc agagcgagta tatataggac
     4861 taaaaaatga cgtaacggtt aaagtccaca aaaaacaccc agaaaaccgc acgcgaacct
     4921 acgcccagaa acgaaagcca aaaaacccac aacttcctca aatcgtcact tccgttttcc
     4981 cacgttacgt aacttcccat tttaagaaaa ctacaattcc caacacatac aagttactcc
     5041 gccctaaaac ctacgtcacc cgccccgttc ccacgccccg cgccacgtca caaactccac
     5101 cccctcatta tcatattggc ttcaatccaa aataaggtat a
//

载体质粒图谱pdf版和相关资料下载

载体应用举例

分享到:

全部载体分类

相关载体

相关文章

相关问答

Copyright © 20012-2013 BIOFENG. 生物风 版权所有 Powered by Biofeng
欢迎您的访问和咨询....