| 质粒名称: | pLH-sgRNA1-2XPP7 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 75390 |
| 存储实验室: | Thoru Pederson |
|
载体骨架基本信息 |
|
| 载体名称: | pLKO.1 |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 8000 bp |
| 完整质粒大小: | 8313 bp |
| 载体类型: | 哺乳动物表达，慢病毒，CRISPR |
| 筛选标记: | 潮霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | sgRNA1-2XPP7 |
| 物种来源: | Synthetic |
| 插入大小: | 122 bp |
| 启动子 | U6 |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | Sbf I (未破坏) |
| 3' 克隆位点: | EcoR I(未破坏) |
| 5' 测序引物 | cccttcaccgagggcctatttccc |
| 3' 测序引物 | CTATTCTTTCCCCTGCACTGTACCC |
|
文献引用方法 |
|
| 材料和方法部分: | pLH-sgRNA1-2XPP7 was a gift from Thoru Pederson (Addgene plasmid # 75390 ; http://n2t.net/addgene:75390 ; RRID:Addgene_75390) |
| 参考文献部分: | Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T. Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526. 10.1038/nbt.3526 PubMed 27088723 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLH-sgRNA1-2XPP7 8313 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION sgRNA1-2XPP7.
ACCESSION .
VERSION .
KEYWORDS pLH-sgRNA1-2XPP7
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8313)
AUTHORS Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T
TITLE Multiplexed labeling of genomic loci with dCas9 and engineered
sgRNAs using CRISPRainbow.
JOURNAL Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
PUBMED 27088723
REFERENCE 2 (bases 1 to 8313)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8313
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 230..470
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
primer_bind 230..250
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 401..420
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
CDS 819..1124
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 1003..1022
/label=ccdB-fwd
/note="ccdB gene, forward primer"
misc_feature 1315..1432
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 1481..1991
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind 1910..1928
/label=hPGK-F
/note="Human PGK promoter, forward primer"
CDS 2081..3106
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
E"
primer_bind complement(3130..3146)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3131..3147)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 3229..3462
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 3534..3655
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3571..3590)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 3625..3644
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 3695..3830
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3757..3776
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(3850..3869)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(3851..3869)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(3879..3896)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(3879..3895)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3888..3910)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 4037..4492
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4124..4143)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4334..4355
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 4518..4622
/gene="bla"
/label=AmpR promoter
CDS 4623..5483
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4841..4860)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 5654..6242
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6143..6162
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 6396..6413
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 6530..6551
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 6566..6596
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6604..6620
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 6609..6631
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 6628..6644
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 6628..6644
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 6663..6683
/label=T3
/note="T3 promoter, forward primer"
promoter 6665..6683
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 6711..6937
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 6938..7118
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 7165..7290
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 7783..8016
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
ORIGIN
1 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
61 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
121 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
181 tcgacggtat cgatcacgag actagcctcg agcggccgcc cccttcaccg agggcctatt
241 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattggaat
301 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt
361 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt
421 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgg
481 tgtcttcgag gcttactaaa agccagataa cagtatgcgt atttgcgcgc tgatttttgc
541 ggtataagaa tatatactga tatgtatacc cgaagtatgt caaaaagagg tgtgctatga
601 agcagcgtat tacagtgaca gttgacagcg acagctatca gttgctcaag gcatatatga
661 tgtcaatatc tccggtctgg taagcacaac catgcagaat gaagcccgtc gtctgcgtgc
721 cgaacgctgg aaagcggaaa atcaggaagg gatggctgag gtcgcccggt ttattgaaat
781 gaacggctct tttgctgacg agaacaggga ctggtgaaat gcagtttaag gtttacacct
841 ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc
901 ccgggcgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtctccc
961 gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata
1021 tggccagtgt gccggtctcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa
1081 atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaaccctg caggacgaag
1141 acccgtttga gagctaccgg agcagacgat atggcgtcgc tccggtagca agttcaaata
1201 aggctagtcc gttatcaact tggagcagac gatatggcgt cgctccaagt ggcaccgagt
1261 cggtgctttt tttgaattct cgacctcgag acaaatggca gtattcatcc acaattttaa
1321 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
1381 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
1441 ttacagggac agcagagatc cactttggcc gcggctcgag ggggttgggg ttgcgccttt
1501 tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt tccgggaaac
1561 gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga
1621 tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg
1681 aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta
1741 gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg cgatgggctg
1801 tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc
1861 gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat
1921 tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga atcaccgacc
1981 tctctcccca gggggatcca ccggatcaag cttgccagct ggggcgccct ctggtaaggt
2041 tgggaagccc tgcagatctg atcccggggg gcaatgagat atgaaaaagc ctgaactcac
2101 cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
2161 gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt
2221 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt
2281 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattta gcgagagcct
2341 gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagatctgc ctgaaaccga
2401 actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct
2461 tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg
2521 gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga
2581 cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga
2641 ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga
2701 caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata
2761 cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg
2821 ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct
2881 ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc
2941 ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac
3001 acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga
3061 tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaatagagta gatgccgacc
3121 gggatctatc gataccgtcg acctcgaggg ggggcccggt acctttaaga ccaatgactt
3181 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
3241 ttcactccca acgaagaaaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
3301 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
3361 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
3421 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
3481 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
3541 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
3601 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
3661 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
3721 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
3781 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
3841 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
3901 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
3961 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
4021 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
4081 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
4141 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
4201 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
4261 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
4321 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
4381 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
4441 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
4501 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
4561 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
4621 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
4681 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
4741 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
4801 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
4861 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
4921 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
4981 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
5041 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
5101 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
5161 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
5221 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
5281 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
5341 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
5401 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
5461 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
5521 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
5581 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
5641 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
5701 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
5761 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
5821 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
5881 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
5941 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
6001 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
6061 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
6121 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
6181 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
6241 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
6301 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
6361 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
6421 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
6481 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
6541 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
6601 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
6661 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
6721 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
6781 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
6841 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
6901 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
6961 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
7021 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
7081 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
7141 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
7201 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
7261 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
7321 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
7381 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
7441 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
7501 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
7561 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
7621 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
7681 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
7741 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
7801 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
7861 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
7921 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
7981 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
8041 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
8101 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
8161 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
8221 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
8281 tataaaatta ttcataatga tagtaggagg ctt
//
