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载体序列
LOCUS pcDNA3.1+ N-Myc 5453 bp ds-DNA circular SYN 21-MAY-2015
DEFINITION Mammalian vector with a CMV promoter for expressing N-terminally
Myc-tagged proteins.
ACCESSION .
VERSION .
KEYWORDS pcDNA3.1+ N-Myc
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5453)
AUTHORS GenScript
TITLE Direct Submission
JOURNAL Exported Tuesday, February 4, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5453
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer 235..614
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 863..881
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 923..925
/codon_start=1
/product="start codon"
/note="ATG"
/translation="M"
CDS 926..955
/codon_start=1
/product="Myc (human c-Myc oncogene) epitope tag"
/note="Myc"
/translation="EQKLISEEDL"
misc_feature 956..1024
/note="MCS"
polyA_signal 1050..1274
/note="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
rep_origin 1320..1748
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1762..2091
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 1942..2077
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 2158..2952
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3126..3247
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(3698..4286)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4457..5317)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5318..5422)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttgcca ccatggagca gaaactcatc tctgaagagg atctgggtac
961 cgagctcgga tccgaattct gcagatatcc agcacagtgg cggccgctcg agtctagagg
1021 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
1081 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
1141 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
1201 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
1261 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
1321 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
1381 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
1441 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
1501 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
1561 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
1621 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
1681 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
1741 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
1801 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
1861 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
1921 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
1981 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
2041 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
2101 ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg
2161 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
2221 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
2281 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
2341 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
2401 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
2461 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
2521 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
2581 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
2641 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
2701 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
2761 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
2821 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
2881 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
2941 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
3001 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
3061 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
3121 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
3181 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
3241 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
3301 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
3361 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
3421 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
3481 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
3541 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
3601 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
3661 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
3721 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
3781 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
3841 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
3901 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
3961 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
4021 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
4081 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
4141 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
4201 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
4261 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
4321 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
4381 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
4441 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
4501 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
4561 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
4621 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
4681 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
4741 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
4801 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
4861 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
4921 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
4981 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
5041 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
5101 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
5161 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
5221 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
5281 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
5341 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
5401 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc
//
