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pcDNA3.1+N-MYC载体质粒基本信息
pcDNA3.1+N-MYC质粒图谱载体图谱和pcDNA3.1+N-MYC载体序列质粒序列多克隆位点信息
pcDNA3.1+N-MYC质粒载体简介
pcDNA3.1+N-MYC质粒序列载体序列
LOCUS pcDNA3.1+ N-Myc 5453 bp ds-DNA circular SYN 21-MAY-2015 DEFINITION Mammalian vector with a CMV promoter for expressing N-terminally Myc-tagged proteins. ACCESSION . VERSION . KEYWORDS pcDNA3.1+ N-Myc SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5453) AUTHORS GenScript TITLE Direct Submission JOURNAL Exported Tuesday, February 4, 2020 from SnapGene Viewer 3.1.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5453 /organism="synthetic DNA construct" /lab_host="Mammalian Cells" /mol_type="other DNA" enhancer 235..614 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" promoter 863..881 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" CDS 923..925 /codon_start=1 /product="start codon" /note="ATG" /translation="M" CDS 926..955 /codon_start=1 /product="Myc (human c-Myc oncogene) epitope tag" /note="Myc" /translation="EQKLISEEDL" misc_feature 956..1024 /note="MCS" polyA_signal 1050..1274 /note="bGH poly(A) signal" /note="bovine growth hormone polyadenylation signal" rep_origin 1320..1748 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 1762..2091 /note="SV40 promoter" /note="SV40 enhancer and early promoter" rep_origin 1942..2077 /note="SV40 ori" /note="SV40 origin of replication" CDS 2158..2952 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 3126..3247 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" rep_origin complement(3698..4286) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4457..5317) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(5318..5422) /gene="bla" /note="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttgcca ccatggagca gaaactcatc tctgaagagg atctgggtac 961 cgagctcgga tccgaattct gcagatatcc agcacagtgg cggccgctcg agtctagagg 1021 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt 1081 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta 1141 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg 1201 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc 1261 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca 1321 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc 1381 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac 1441 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag 1501 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc 1561 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg 1621 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata 1681 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa 1741 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 1801 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc 1861 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata 1921 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 1981 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag 2041 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg 2101 ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg 2161 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc 2221 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg 2281 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag 2341 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc 2401 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat 2461 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg 2521 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc 2581 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag 2641 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc 2701 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc 2761 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata 2821 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc 2881 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac 2941 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc 3001 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt 3061 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc 3121 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 3181 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 3241 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat 3301 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa 3361 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc 3421 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 3481 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 3541 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 3601 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 3661 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 3721 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 3781 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 3841 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 3901 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 3961 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 4021 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 4081 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa 4141 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 4201 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 4261 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 4321 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 4381 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 4441 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 4501 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 4561 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 4621 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 4681 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 4741 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 4801 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 4861 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 4921 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 4981 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 5041 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 5101 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 5161 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 5221 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 5281 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 5341 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 5401 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc //