质粒名称: | FUGW-PercevalHR |
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出品公司: | addgene |
目录编号: | 49083 |
存储实验室: | Gary Yellen |
载体骨架基本信息 |
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载体名称: | FUGW |
空载体大小: | 9222bp |
完整质粒大小: | 10962 bp |
载体类型: | 哺乳细胞表达 |
载体抗性: | 氨苄西林 |
生长温度: | 37 ℃ |
宿主菌: | Stbl3 |
拷贝数: | 高拷贝 |
插入基因信息 |
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插入基因名称: | PercevalHR |
插入大小: | 1740bp |
文献引用方法 |
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材料和方法部分: | FUGW-PercevalHR was a gift from Gary Yellen (Addgene plasmid # 49083 ; http://n2t.net/addgene:49083 ; RRID:Addgene_49083) |
参考文献部分: | Imaging energy status in live cells with a fluorescent biosensor of the intracellular ATP-to-ADP ratio. Tantama M, Martinez-Francois JR, Mongeon R, Yellen G. Nat Commun. 2013;4:2550. doi: 10.1038/ncomms3550. 10.1038/ncomms3550 PubMed 24096541 |
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载体基本信息
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载体序列
LOCUS FUGW-PercevalHR 10974 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral vector for PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.. ACCESSION . VERSION . KEYWORDS FUGW-PercevalHR SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10974) AUTHORS Tantama M, Martinez-Francois JR, Mongeon R, Yellen G TITLE Imaging energy status in live cells with a fluorescent biosensor of the intracellular ATP-to-ADP ratio. JOURNAL Nat Commun. 2013;4:2550. doi: 10.1038/ncomms3550. PUBMED 24096541 REFERENCE 2 (bases 1 to 10974) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.biofeng.com FEATURES Location/Qualifiers source 1..10974 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(47..66) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 238..617 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 619..817 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 772..792 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 835..1015 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1680..1913 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2098..2142 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2440..2557 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2622..3833 /label=UbC promoter /note="human ubiquitin C promoter" primer_bind 3670..3689 /label=hUBCpro-F /note="Human Ubiquitin C (UbC) promoter, forward primer" CDS 4059..4310 /codon_start=1 /product="C-terminal fragment of mVenus for use in bimolecular fluorescence complementation (BiFC) (Kodama and Hu, 2010)" /label=VC155 /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH YLSFQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK" primer_bind 4247..4268 /label=EGFP-C /note="EGFP, forward primer" primer_bind complement(4371..4392) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(4632..4651) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" misc_feature 5666..6254 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5719..5739) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6137..6148) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(6257..6273) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6258..6274) /label=pBluescriptKS /note="For pBluescript vector" LTR 6779..6959 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(6985..7002) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6991..7215 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 7261..7689 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7348..7367) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7558..7579 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(7698..7718) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 7703..8032 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 7883..8018 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7945..7964 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 8080..8127 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 8146..8520 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 8650..8771 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8687..8706) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8741..8760 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(8820..8836) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8820..8836) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8833..8855) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8844..8860 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8868..8898) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8913..8934 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(9051..9068) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(9222..9810) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(9302..9321) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9981..10841) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10604..10623 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10842..10946) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta acccgtgtcg gctccagatc tggcctccgc gccgggtttt 2641 ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg 2701 cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga 2761 ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc 2821 tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg 2881 cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc 2941 gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga 3001 tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc 3061 gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt 3121 ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt 3181 cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg 3241 ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc 3301 ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg 3361 actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc 3421 agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt 3481 tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc 3541 gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct 3601 ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc 3661 ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg 3721 aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt 3781 agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta gacgaagctt 3841 gggctgcagg tcgactctag accaccatga aaaaggttga atccatcatc aggcccgaaa 3901 agctggagat cgttaagaag gctctctcgg acgctggata tgtgggtatg accgtctctg 3961 aagtcaaagg ctctggcgtc cagggcggca tcttcgagag gtaccgagga agggtgtact 4021 ctgcaggcta caacagcgac aacgtctaca tcaccgccga caagcagaag aacggcatca 4081 aggccaactt caagatccgc cacaacatcg aggacggcgg cgtgcagctc gccgaccact 4141 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga 4201 gcttccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg 4261 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag ggcggttccg 4321 gaggcatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc 4381 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca 4441 cctacggcaa gctgaccctg aagctgatct gcaccaccgg caagctgccc gtgccctggc 4501 ccaccctcgt gaccaccctg ggctacggtc tccaatgctt cgcccgctac cccgaccaca 4561 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca 4621 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca 4681 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg 4741 ggcacaagct tgagtacaac ggcaccatag tagatctgat ccctaaggta aaaattgaac 4801 tagtggtgaa ggaggaggac gtcgataacg tgatagacat tatttgcgaa aatgcccgca 4861 caggtaaccc cggcgacggt aagatatttg tgatcccagt ggagcgagtg gtcagggtgc 4921 gaaccaaaga ggagggagca tctggtggtg gatccggtgg tggcggtgca tctggtatga 4981 aaaaggttga agctattatt cgcccagaga aactggagat tgttaagaag gccctgtccg 5041 acgccggcta tgttggcatg accgtttccg aggttaaagg ccgcggtgcc ggcggtggcg 5101 acctgattcc taaggtgaag atcgagctgg ttgttaagga ggaagacgtt gataatgtta 5161 tcgacattat ctgcgagaac gcccgcactg gcaatcctgg tgacggcaaa atcttcgtta 5221 taccggtcga gcgaattgtg cgagtacgaa ccaaagaaga gggtgcctcc ggcggtggcg 5281 gtggctccgg tggcgccagc ggcatgaaga aggttgaggc catcatccgc ccagagaagc 5341 tggaaatcgt taagaaggcc ttaaacgacg acggctacgt cggcatgaca gttagcgagg 5401 ttaaaggtcg cggtgccggt gggggtgatc tgattcctaa ggttaaaatc gagctcgttg 5461 ttaaggagga ggacgttgac aacattatcg atatcatctg cgaaaacgct cgcaccggca 5521 acccaggcga cggcaagatt ttcgtcattc ctgttgagag agtggtgcgt ccccgtacga 5581 aggaggaggg caaggaagca ctgtgactcg acgaattctt cgaagctagc ggatccgtta 5641 acaattcgat atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt 5701 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc 5761 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg 5821 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac 5881 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc 5941 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc 6001 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa 6061 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc 6121 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc 6181 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg 6241 ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg gagcaatcac 6301 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga 6361 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc 6421 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca 6481 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg 6541 gcagaactac acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa 6601 gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt 6661 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag 6721 gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg 6781 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact 6841 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg 6901 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag 6961 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg 7021 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct 7081 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg 7141 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg 7201 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc 7261 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 7321 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 7381 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 7441 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 7501 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 7561 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 7621 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 7681 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc 7741 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc 7801 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat 7861 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 7921 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga 7981 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc 8041 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca 8101 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc 8161 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac 8221 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac 8281 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg 8341 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac 8401 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag 8461 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga 8521 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc 8581 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc 8641 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 8701 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 8761 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg 8821 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 8881 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 8941 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 9001 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 9061 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 9121 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 9181 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 9241 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 9301 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 9361 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 9421 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 9481 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 9541 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 9601 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 9661 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 9721 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 9781 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 9841 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 9901 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 9961 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 10021 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 10081 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 10141 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 10201 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 10261 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 10321 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 10381 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 10441 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 10501 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 10561 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 10621 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 10681 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 10741 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 10801 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 10861 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 10921 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgac //
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