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pCMV6-AC-IRES-GFP载体质粒基本信息
pCMV6-AC-IRES-GFP质粒图谱载体图谱和pCMV6-AC-IRES-GFP载体序列质粒序列多克隆位点信息
pCMV6-AC-IRES-GFP质粒载体简介
pCMV6-AC-IRES-GFP质粒序列载体序列
LOCUS pCMV6-AC-IRES-GFP 7272 bp ds-DNA circular SYN 14-FEB-2014
DEFINITION PrecisionShuttle(TM) mammalian expression vector with a TurboGFP
marker, for fusing an ORF to C-terminal c-Myc and FLAG(R) tags.
ACCESSION .
VERSION .
KEYWORDS pCMV6-AC-IRES-GFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7272)
AUTHORS OriGene
TITLE Direct Submission
JOURNAL Exported Saturday, June 13, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
COMMENT Inserts are typically cloned between the SgfI (AsiSI) and MluI
sites.
FEATURES Location/Qualifiers
source 1..7272
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer 343..722
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 723..926
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 952..970
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 979..1087
/note="MCS"
/note="multiple cloning site"
CDS 1085..1114
/codon_start=1
/product="Myc (human c-Myc oncogene) epitope tag"
/note="Myc"
/translation="EQKLISEEDL"
CDS 1133..1156
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/note="FLAG"
/translation="DYKDDDDK"
misc_feature 1184..1770
/note="IRES2"
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS 1759..1761
/codon_start=1
/product="start codon"
/note="ATG"
/translation="M"
CDS 1771..2469
/codon_start=1
/product="improved green fluorescent protein from
Pontellina plumata (Evdokimov et al., 2006)"
/note="TurboGFP"
/note="mammalian codon-optimized"
/translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR
TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF
KTPDADAGEE"
polyA_signal 2505..3127
/note="hGH poly(A) signal"
/note="human growth hormone polyadenylation signal"
promoter 3156..3485
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 3336..3471
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 3552..4346
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 4520..4641
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(5092..5679)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5850..6710)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6711..6815)
/gene="bla"
/note="AmpR promoter"
rep_origin 6842..25
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
1021 cgatcgccgg cgcgccagat ctcaagctta actagctagc ggaccgacgc gtacgcggcc
1081 gctcgagcag aaactcatct cagaagagga tctggcagca aatgatatcc tggattacaa
1141 ggatgacgac gataaggttt aaacgtagcg gccgcaaatt ccgcccctct ccctcccccc
1201 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
1261 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
1321 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
1381 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
1441 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
1501 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
1561 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
1621 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
1681 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
1741 gaaaaacacg atgataatat ggccacaacc atggagagcg acgagagcgg cctgcccgcc
1801 atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc
1861 ggcggagagg gcacccccga gcagggccgc atgaccaaca agatgaagag caccaaaggc
1921 gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc
1981 ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac
2041 accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac
2101 cgctacgagg ccggccgcgt gatcggcgac ttcaaggtga tgggcaccgg cttccccgag
2161 gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac
2221 cccatgggcg ataacgatct ggatggcagc ttcacccgca ccttcagcct gcgcgacggc
2281 ggctactaca gctccgtggt ggacagccac atgcacttca agagcgccat ccaccccagc
2341 atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagga tcacagcaac
2401 accgagctgg gcatcgtgga gtaccagcac gccttcaaga ccccggatgc agatgccggt
2461 gaagaataat agccgcggtc atagctgttt cctgaacaga tcccgggtgg catccctgtg
2521 acccctcccc agtgcctctc ctggccctgg aagttgccac tccagtgccc accagccttg
2581 tcctaataaa attaagttgc atcattttgt ctgactaggt gtccttctat aatattatgg
2641 ggtggagggg ggtggtatgg agcaaggggc aagttgggaa gacaacctgt agggcctgcg
2701 gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact gcaatctccg
2761 cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga ttccaggcat
2821 gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc accatattgg
2881 ccaggctggt ctccaactcc taatctcagg tgatctaccc accttggcct cccaaattgc
2941 tgggattaca ggcgtgaacc actgctccct tccctgtcct tctgatttta aaataactat
3001 accagcagga ggacgtccag acacagcata ggctacctgg ccatgcccaa ccggtgggac
3061 atttgagttg cttgcttggc actgtcctct catgcgttgg gtccactcag tagatgcctg
3121 ttgaattggg tacgcggcca gcttggctgt ggaatgtgtg tcagttaggg tgtggaaagt
3181 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
3241 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
3301 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
3361 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
3421 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
3481 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag
3541 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
3601 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
3661 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
3721 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
3781 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
3841 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
3901 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
3961 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
4021 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
4081 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
4141 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggccgacc
4201 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
4261 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
4321 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
4381 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
4441 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
4501 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
4561 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
4621 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
4681 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
4741 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
4801 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
4861 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
4921 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
4981 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
5041 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
5101 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
5161 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
5221 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
5281 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
5341 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
5401 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
5461 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
5521 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
5581 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gttttttgtt
5641 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
5701 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
5761 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
5821 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
5881 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
5941 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
6001 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
6061 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
6121 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
6181 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
6241 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
6301 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
6361 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
6421 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
6481 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
6541 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
6601 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
6661 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
6721 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
6781 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
6841 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
6901 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
6961 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
7021 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
7081 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
7141 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
7201 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
7261 aacgcgaatt tt
//
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