质粒名称: | pHR-SFFV-dCas9-BFP-KRAB |
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出品公司: | 爱迪基因 |
目录编号: | 46911 |
存储实验室: | Stanley Qi, Jonathan Weissman |
载体骨架基本信息 |
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载体名称: | pHR |
载体出品公司: | Addgene |
载体抗性: | 氨苄霉素 |
空载体大小: | 9000 bp |
完整质粒大小: | 14167 bp |
载体类型: | 哺乳细胞表达 |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | dCas9-BFP-KRAB fusion |
物种来源: | H. sapiens (human) |
插入大小: | 5172 bp |
启动子 | SFFV |
标签/融合蛋白: | HA(C端) 2xNLS(C端) BFP(C端) KRAB domain(C端) |
克隆方法: | Ligation Independent Cloning |
5' 测序引物 | gcttcccgagctctataaaagag |
3' 测序引物 | CCAGAGGTTGATTATCGATAAGC |
文献引用方法 |
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材料和方法部分: | pHR-SFFV-dCas9-BFP-KRAB was a gift from Stanley Qi & Jonathan Weissman (Addgene plasmid # 46911) |
参考文献部分: | CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, Weissman JS, Qi LS. Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044. 10.1016/j.cell.2013.06.044 PubMed 23849981 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pHR-SFFV-dCas9-BFP-KRAB 14167 bp DNA SYN DEFINITION pHR-SFFV-dCas9-BFP-KRAB ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..14167 /organism="pHR-SFFV-dCas9-BFP-KRAB" /mol_type="other DNA" misc_feature 454..634 /label="HIV-1_5_LTR" misc_feature 454..634 /label="truncHIV-1_3_LTR" misc_feature 745..789 /label="HIV-1_psi_pack" CDS complement(1015..1476) /label="ORF frame 2" misc_feature 1303..1535 /label="RRE" CDS 1642..2241 /label="ORF frame 1" misc_feature 2053..2068 /label="cPPT" CDS complement(2675..7543) /label="ORF frame 1" CDS 2801..7969 /label="ORF frame 2" misc_feature 6908..6934 /label="HA" misc_feature 6953..6973 /label="SV40 NLS" misc_feature 6959..6973 /label="NLS" misc_feature 8032..8619 /label="WPRE" CDS 8120..8647 /label="ORF frame 2" CDS 8133..8807 /label="ORF frame 3" misc_feature complement(8622..8638) /label="pBluescriptKS_primer" misc_feature 8711..8726 /label="cPPT" misc_feature 8711..8732 /label="U3PPT" misc_feature 8728..8780 /label="delta_U3" misc_feature 8781..8961 /label="HIV-1_5_LTR" misc_feature 8781..8961 /label="truncHIV-1_3_LTR" misc_feature complement(8987..9004) /label="Sp6_primer" gene 9242..9505 /label="tet (300 - 563)" /gene="tet (300 - 563)" misc_feature complement(9294..9313) /label="pBRrevBam_primer" misc_feature 9646..9668 /label="pGEX_3_primer" promoter 9827..9855 /label="AmpR_promoter" gene 9897..10757 /label="Ampicillin" /gene="Ampicillin" CDS 9897..10757 /label="ORF frame 3" rep_origin 10912..11531 /label="pBR322_origin" misc_feature complement(11757..11777) /label="pBABE_3_primer" misc_feature complement(11763..12216) /label="SV40_enhancer" promoter 11775..12043 /label="SV40_promoter" rep_origin 11942..12019 /label="SV40_origin" misc_feature 12004..12023 /label="SV40pro_F_primer" misc_feature 13224..13239 /label="SV40_int" misc_feature 13245..13292 /label="SV40_3_splice" CDS 13369..13842 /label="ORF frame 1" misc_feature 13420..13440 /label="SV40 NLS" misc_feature 13426..13440 /label="NLS" terminator 13868..13999 /label="SV40_PA_terminator" misc_feature 13956..13975 /label="EBV_rev_primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtgaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa 1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag 1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta 1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg 1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat 1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta 1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc 1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac 1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccaaat ggcagtattc 2041 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta 2101 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa 2161 aattttcggg tttattacag ggacagcaga gatccagttt ggatcgataa gcttgatatc 2221 gaattcctgc agccccgata aaataaaaga ttttatttag tctccagaaa aaggggggaa 2281 tgaaagaccc cacctgtagg tttggcaagc tagctgcagt aacgccattt tgcaaggcat 2341 ggaaaaatac caaaccaaga atagagaagt tcagatcaag ggcgggtaca tgaaaatagc 2401 taacgttggg ccaaacagga tatctgcggt gagcagtttc ggccccggcc cggggccaag 2461 aacagatggt caccgcagtt tcggccccgg cccgaggcca agaacagatg gtccccagat 2521 atggcccaac cctcagcagt ttcttaagac ccatcagatg tttccaggct cccccaagga 2581 cctgaaatga ccctgcgcct tatttgaatt aaccaatcag cctgcttctc gcttctgttc 2641 gcgcgcttct gcttcccgag ctctataaaa gagctcacaa cccctcactc ggcgcgccag 2701 tcctccgaca gactgagtcg cccggggggg atctggagct ctcgagaatt ctcacgcgtc 2761 tgcaggatat caagcttgcg gtaccgcggg cccgGCCACC ATGGACAAGA AGTACAGCAT 2821 CGGCCTGGCC ATCGGCACCA ACTCTGTGGG CTGGGCCGTG ATCACCGACG AGTACAAGGT 2881 GCCCAGCAAG AAATTCAAGG TGCTGGGCAA CACCGACCGG CACAGCATCA AGAAGAACCT 2941 GATCGGCGCC CTGCTGTTCG ACAGCGGAGA AACAGCCGAG GCCACCCGGC TGAAGAGAAC 3001 CGCCAGAAGA AGATACACCA GACGGAAGAA CCGGATCTGC TATCTGCAAG AGATCTTCAG 3061 CAACGAGATG GCCAAGGTGG ACGACAGCTT CTTCCACAGA CTGGAAGAGT CCTTCCTGGT 3121 GGAAGAGGAT AAGAAGCACG AGCGGCACCC CATCTTCGGC AACATCGTGG ACGAGGTGGC 3181 CTACCACGAG AAGTACCCCA CCATCTACCA CCTGAGAAAG AAACTGGTGG ACAGCACCGA 3241 CAAGGCCGAC CTGCGGCTGA TCTATCTGGC CCTGGCCCAC ATGATCAAGT TCCGGGGCCA 3301 CTTCCTGATC GAGGGCGACC TGAACCCCGA CAACAGCGAC GTGGACAAGC TGTTCATCCA 3361 GCTGGTGCAG ACCTACAACC AGCTGTTCGA GGAAAACCCC ATCAACGCCA GCGGCGTGGA 3421 CGCCAAGGCC ATCCTGTCTG CCAGACTGAG CAAGAGCAGA CGGCTGGAAA ATCTGATCGC 3481 CCAGCTGCCC GGCGAGAAGA AGAATGGCCT GTTCGGCAAC CTGATTGCCC TGAGCCTGGG 3541 CCTGACCCCC AACTTCAAGA GCAACTTCGA CCTGGCCGAG GATGCCAAAC TGCAGCTGAG 3601 CAAGGACACC TACGACGACG ACCTGGACAA CCTGCTGGCC CAGATCGGCG ACCAGTACGC 3661 CGACCTGTTT CTGGCCGCCA AGAACCTGTC CGACGCCATC CTGCTGAGCG ACATCCTGAG 3721 AGTGAACACC GAGATCACCA AGGCCCCCCT GAGCGCCTCT ATGATCAAGA GATACGACGA 3781 GCACCACCAG GACCTGACCC TGCTGAAAGC TCTCGTGCGG CAGCAGCTGC CTGAGAAGTA 3841 CAAAGAGATT TTCTTCGACC AGAGCAAGAA CGGCTACGCC GGCTACATCG ATGGCGGAGC 3901 CAGCCAGGAA GAGTTCTACA AGTTCATCAA GCCCATCCTG GAAAAGATGG ACGGCACCGA 3961 GGAACTGCTC GTGAAGCTGA ACAGAGAGGA CCTGCTGCGG AAGCAGCGGA CCTTCGACAA 4021 CGGCAGCATC CCCCACCAGA TCCACCTGGG AGAGCTGCAC GCCATTCTGC GGCGGCAGGA 4081 AGATTTTTAC CCATTCCTGA AGGACAACCG GGAAAAGATC GAGAAGATCC TGACCTTCCG 4141 CATCCCCTAC TACGTGGGCC CTCTGGCCAG GGGAAACAGC AGATTCGCCT GGATGACCAG 4201 AAAGAGCGAG GAAACCATCA CCCCCTGGAA CTTCGAGGAA GTGGTGGACA AGGGCGCCAG 4261 CGCCCAGAGC TTCATCGAGC GGATGACCAA CTTCGATAAG AACCTGCCCA ACGAGAAGGT 4321 GCTGCCCAAG CACAGCCTGC TGTACGAGTA CTTCACCGTG TACAACGAGC TGACCAAAGT 4381 GAAATACGTG ACCGAGGGAA TGAGAAAGCC CGCCTTCCTG AGCGGCGAGC AGAAAAAAGC 4441 CATCGTGGAC CTGCTGTTCA AGACCAACCG GAAAGTGACC GTGAAGCAGC TGAAAGAGGA 4501 CTACTTCAAG AAAATCGAGT GCTTCGACTC CGTGGAAATC TCCGGCGTGG AAGATCGGTT 4561 CAACGCCTCC CTGGGCACAT ACCACGATCT GCTGAAAATT ATCAAGGACA AGGACTTCCT 4621 GGACAATGAG GAAAACGAGG ACATTCTGGA AGATATCGTG CTGACCCTGA CACTGTTTGA 4681 GGACAGAGAG ATGATCGAGG AACGGCTGAA AACCTATGCC CACCTGTTCG ACGACAAAGT 4741 GATGAAGCAG CTGAAGCGGC GGAGATACAC CGGCTGGGGC AGGCTGAGCC GGAAGCTGAT 4801 CAACGGCATC CGGGACAAGC AGTCCGGCAA GACAATCCTG GATTTCCTGA AGTCCGACGG 4861 CTTCGCCAAC AGAAACTTCA TGCAGCTGAT CCACGACGAC AGCCTGACCT TTAAAGAGGA 4921 CATCCAGAAA GCCCAGGTGT CCGGCCAGGG CGATAGCCTG CACGAGCACA TTGCCAATCT 4981 GGCCGGCAGC CCCGCCATTA AGAAGGGCAT CCTGCAGACA GTGAAGGTGG TGGACGAGCT 5041 CGTGAAAGTG ATGGGCCGGC ACAAGCCCGA GAACATCGTG ATCGAAATGG CCAGAGAGAA 5101 CCAGACCACC CAGAAGGGAC AGAAGAACAG CCGCGAGAGA ATGAAGCGGA TCGAAGAGGG 5161 CATCAAAGAG CTGGGCAGCC AGATCCTGAA AGAACACCCC GTGGAAAACA CCCAGCTGCA 5221 GAACGAGAAG CTGTACCTGT ACTACCTGCA GAATGGGCGG GATATGTACG TGGACCAGGA 5281 ACTGGACATC AACCGGCTGT CCGACTACGA TGTGGACGCT ATCGTGCCTC AGAGCTTTCT 5341 GAAGGACGAC TCCATCGATA ACAAAGTGCT GACTCGGAGC GACAAGAACC GGGGCAAGAG 5401 CGACAACGTG CCCTCCGAAG AGGTCGTGAA GAAGATGAAG AACTACTGGC GCCAGCTGCT 5461 GAATGCCAAG CTGATTACCC AGAGGAAGTT CGACAATCTG ACCAAGGCCG AGAGAGGCGG 5521 CCTGAGCGAA CTGGATAAGG CCGGCTTCAT CAAGAGACAG CTGGTGGAAA CCCGGCAGAT 5581 CACAAAGCAC GTGGCACAGA TCCTGGACTC CCGGATGAAC ACTAAGTACG ACGAGAACGA 5641 CAAACTGATC CGGGAAGTGA AAGTGATCAC CCTGAAGTCC AAGCTGGTGT CCGATTTCCG 5701 GAAGGATTTC CAGTTTTACA AAGTGCGCGA GATCAACAAC TACCACCACG CCCACGACGC 5761 CTACCTGAAC GCCGTCGTGG GAACCGCCCT GATCAAAAAG TACCCTAAGC TGGAAAGCGA 5821 GTTCGTGTAC GGCGACTACA AGGTGTACGA CGTGCGGAAG ATGATCGCCA AGAGCGAGCA 5881 GGAAATCGGC AAGGCTACCG CCAAGTACTT CTTCTACAGC AACATCATGA ACTTTTTCAA 5941 GACCGAGATT ACCCTGGCCA ACGGCGAGAT CCGGAAGCGG CCTCTGATCG AGACAAACGG 6001 CGAAACAGGC GAGATCGTGT GGGATAAGGG CCGGGACTTT GCCACCGTGC GGAAAGTGCT 6061 GTCTATGCCC CAAGTGAATA TCGTGAAAAA GACCGAGGTG CAGACAGGCG GCTTCAGCAA 6121 AGAGTCTATC CTGCCCAAGA GGAACAGCGA CAAGCTGATC GCCAGAAAGA AGGACTGGGA 6181 CCCTAAGAAG TACGGCGGCT TCGACAGCCC CACCGTGGCC TATTCTGTGC TGGTGGTGGC 6241 CAAAGTGGAA AAGGGCAAGT CCAAGAAACT GAAGAGTGTG AAAGAGCTGC TGGGGATCAC 6301 CATCATGGAA AGAAGCAGCT TCGAGAAGAA TCCCATCGAC TTTCTGGAAG CCAAGGGCTA 6361 CAAAGAAGTG AAAAAGGACC TGATCATCAA GCTGCCTAAG TACTCCCTGT TCGAGCTGGA 6421 AAACGGCCGG AAGAGAATGC TGGCCTCTGC CGGCGAACTG CAGAAGGGAA ACGAACTGGC 6481 CCTGCCCTCC AAATATGTGA ACTTCCTGTA CCTGGCCAGC CACTATGAGA AGCTGAAGGG 6541 CTCCCCCGAG GATAATGAGC AGAAACAGCT GTTTGTGGAA CAGCACAAAC ACTACCTGGA 6601 CGAGATCATC GAGCAGATCA GCGAGTTCTC CAAGAGAGTG ATCCTGGCCG ACGCTAATCT 6661 GGACAAGGTG CTGAGCGCCT ACAACAAGCA CAGAGACAAG CCTATCAGAG AGCAGGCCGA 6721 GAATATCATC CACCTGTTTA CCCTGACCAA TCTGGGAGCC CCTGCCGCCT TCAAGTACTT 6781 TGACACCACC ATCGACCGGA AGAGGTACAC CAGCACCAAA GAGGTGCTGG ACGCCACCCT 6841 GATCCACCAG AGCATCACCG GCCTGTACGA GACACGGATC GACCTGTCTC AGCTGGGAGG 6901 CGACGCCTAT CCCTATGACG TGCCCGATTA TGCCAGCCTG GGCAGCGGCT CCCCCAAGAA 6961 AAAACGCAAG GTGGAAGATC CTAAGAAAAA GCGGAAAGTG GACGGCATTG GTAGTGGGAG 7021 CAACGGCAGC AGCGGATCCA GCGAGCTGAT TAAGGAGAAC ATGCACATGA AGCTGTACAT 7081 GGAGGGCACC GTGGACAACC ATCACTTCAA GTGCACATCC GAGGGCGAAG GCAAGCCCTA 7141 CGAGGGCACC CAGACCATGA GAATCAAGGT GGTCGAGGGC GGCCCTCTCC CCTTCGCCTT 7201 CGACATCCTG GCTACTAGCT TCCTCTACGG CAGCAAGACC TTCATCAACC ACACCCAGGG 7261 CATCCCCGAC TTCTTCAAGC AGTCCTTCCC TGAGGGCTTC ACATGGGAGA GAGTCACCAC 7321 ATACGAAGAC GGGGGCGTGC TGACCGCTAC CCAGGACACC AGCCTCCAGG ACGGCTGCCT 7381 CATCTACAAC GTCAAGATCA GAGGGGTGAA CTTCACATCC AACGGCCCTG TGATGCAGAA 7441 GAAAACACTC GGCTGGGAGG CCTTCACCGA GACGCTGTAC CCCGCTGACG GCGGCCTGGA 7501 AGGCAGAAAC GACATGGCCC TGAAGCTCGT GGGCGGGAGC CATCTGATCG CAAACATCAA 7561 GACCACATAT AGATCCAAGA AACCCGCTAA GAACCTCAAG ATGCCTGGCG TCTACTATGT 7621 GGACTACAGA CTGGAAAGAA TCAAGGAGGC CAACAACGAG ACCTACGTCG AGCAGCACGA 7681 GGTGGCAGTG GCCAGATACT GCGACCTCCC TAGCAAACTG GGGCACAAGC TTAATGGCGG 7741 TGGCGGAGGG ATGGATGCTA AGTCACTAAC TGCCTGGTCC CGGACACTGG TGACCTTCAA 7801 GGATGTATTT GTGGACTTCA CCAGGGAGGA GTGGAAGCTG CTGGACACTG CTCAGCAGAT 7861 CGTGTACAGA AATGTGATGC TGGAGAACTA TAAGAACCTG GTTTCCTTGG GTTATCAGCT 7921 TACTAAGCCA GATGTGATCC TCCGGTTGGA GAAGGGAGAA GAGCCCTAGT AAggccgcga 7981 ctctagagtc gacctgcagg catgcaagct tgatatcaag cttatcgata atcaacctct 8041 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct 8101 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat 8161 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt 8221 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat 8281 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc 8341 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga 8401 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc 8461 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga 8521 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc 8581 tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgagg 8641 gaattaattc gagctcggta cctttaagac caatgactta caaggcagct gtagatctta 8701 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag 8761 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct 8821 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag 8881 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt 8941 cagtgtggaa aatctctagc agcatctaga attaattccg tgtattctat agtgtcacct 9001 aaatcgtatg tgtatgatac ataaggttat gtattaattg tagccgcgtt ctaacgacaa 9061 tatgtacaag cctaattgtg tagcatctgg cttactgaag cagaccctat catctctctc 9121 gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc tgagtttctg gtaacgccgt 9181 cccgcacccg gaaatggtca gcgaaccaat cagcagggtc atcgctagcc agatcctcta 9241 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat 9301 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt 9361 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca 9421 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct 9481 aatgcaggag tcgcataagg gagagcgtcg aatggtgcac tctcagtaca atctgctctg 9541 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg 9601 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt 9661 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc 9721 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc 9781 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 9841 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga 9901 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt 9961 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag 10021 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag 10081 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta 10141 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg 10201 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca 10261 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag 10321 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc 10381 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg 10441 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc 10501 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg 10561 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg 10621 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga 10681 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac 10741 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa 10801 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 10861 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 10921 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 10981 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 11041 ctggcttcag cagagcgcag ataccaaata ctgtctttct agtgtagccg tagttaggcc 11101 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 11161 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 11221 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 11281 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 11341 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 11401 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 11461 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 11521 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 11581 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata 11641 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc 11701 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa 11761 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag 11821 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag 11881 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc 11941 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 12001 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg 12061 aggctttttt ggaggcctag gcttttgcaa aaagcttgga cacaagacag gcttgcgaga 12121 tatgtttgag aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg 12181 actcatgtga aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt 12241 tcccctcgaa cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa 12301 aatacatcgt cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga 12361 tgccttctga acaatggaaa ggcattattg ccgtaagccg tggcggtctg taccgggtgc 12421 gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga 12481 tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt 12541 catcgttatt gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta 12601 tccaaaagcg cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga 12661 ctatgttgtt gatatcccgc aagatacctg gattgaacag ccgtgggata tgggcgtcgt 12721 attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt 12781 tctttttaac ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca 12841 tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct 12901 cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg 12961 gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca 13021 ttgacccacg cgaaatcctc gacgtccagg cacgtattgt gatgagcgat gccgaacgta 13081 ccgacgatga tttatacgat acggtgattg gctaccgtgg cggcaactgg atttatgagt 13141 gggccccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta 13201 cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa 13261 ctactgattc taattgtttg tgtattttag attccaacct atggaactga tgaatgggag 13321 cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt 13381 gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta 13441 gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt 13501 aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac 13561 aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat 13621 aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct 13681 caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg 13741 tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc 13801 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt 13861 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 13921 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 13981 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc 14041 caccccatac cctattacca ctgccaatta cctagtggtt tcatttactc taaacctgtg 14101 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact 14161 tagtagt //