| 质粒名称: | SspB-mCherry-DHPH-TIAM1 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 176104 |
| 存储实验室: | Dorus Gadella |
|
载体骨架基本信息 |
|
| 载体名称: | pEGFP-C1 |
| 载体出品公司: | Clontech |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | TIAM1 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | SspB-mCherry-DHPH-TIAM1 was a gift from Dorus Gadella (Addgene plasmid # 176104 ; http://n2t.net/addgene:176104 ; RRID:Addgene_176104) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS SspB-mCherry-DHPH-TIAM1 6147 bp ds-DNA circular SYN 27-OCT-2021
DEFINITION Heterodimerization with iLID, Rac1 activation.
ACCESSION .
VERSION .
KEYWORDS SspB-mCherry-DHPH-TIAM1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6147)
TITLE Gadella lab plasmids
REFERENCE 2 (bases 1 to 6147)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Oct 27, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6147
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 519..539
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 949..958
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 955..1662
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(1100..1118)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 1368..1387
/label=mCherry-F
/note="mCherry, forward primer"
polyA_signal 2935..3056
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2972..2991)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 3026..3045
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(3063..3518)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3200..3221)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 3412..3431
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 3545..3649
/gene="bla"
/label=AmpR promoter
promoter 3651..4008
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(3674..3694)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 3859..3994
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3921..3940
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 4043..4837
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4097..4116)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4707..4726
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(5025..5044)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 5069..5116
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 5445..6033
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5934..5953
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagccacc
601 atgagctccc cgaaacgccc taagctgctg cgtgaatatt acgattggct ggttgataac
661 agctttaccc catatctggt ggtggatgcc acatacctgg gcgtgaacgt gcccgtggag
721 tatgtgaaag acggtcagat cgtgctgaat ctgtctgcaa gtgcgaccgg caacctgcaa
781 ctgacaaatg attttatcca gttcaacgcc cgctttaagg gcgtgtctcg tgaactgtat
841 atcccgatgg gtgccgctct ggccatttac gctcgcgaga acggcgatgg tgtgatgttc
901 gaaccagaag aaatctatga cgagctgaat attggtagtg gaccggtcgc caccatggtg
961 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
1021 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
1081 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc
1141 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc
1201 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
1261 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
1321 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
1381 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
1441 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc
1501 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc
1561 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc
1621 gagggccgcc actccaccgg cggcatggac gagctgtaca agtccggact cagatctcga
1681 gaacagctgt cggatgcgga taagctgcgc aaggtgatct gtgaactgct ggagactgaa
1741 cgcacctatg tgaaagactt aaactgcctc atggagagat acctgaagcc ccttcagaag
1801 gagacctttc tcacccagga tgagcttgat gtactttttg gaaatttaac cgaaatggtg
1861 gagtttcaag tcgagttcct taagactcta gaagatggag taagactggt ccctgacttg
1921 gaaaagctgg agaaggttga ccagttcaag aaagtgctct tctctctggg gggatccttc
1981 ctgtactacg cggaccgctt caagctctac agtgccttct gtgcgagcca cacaaaagtc
2041 cccaaggtcc tggtgaaagc caagacggac acagccttca aggcgttcct cgatgcccag
2101 aacccgaggc agcagcactc atccacgctg gagtcttacc tcatcaagcc catccagagg
2161 gtcctcaagt acccgcttct gctcagggag ctgtttgcgc tgaccgatgc ggagagcgag
2221 gaacactacc acctggacgt ggccatcaag actatgaaca aggtcgccag tcacatcaat
2281 gagatgcaga agatccacga agagtttggt gccgtgtttg accagctgat tgctgagcag
2341 acgggagaga agaaagaggt tgcagatctg agcatgggtg acctgctctt gcacaccagc
2401 gtcatctggc tgaacccacc cgcctcactg ggaaagtgga aaaaggagcc agaattagca
2461 gccttcgtct tcaaaacggc cgtggtcctt gtatataaag acggttccaa gcagaagaag
2521 aaacttgttg gctctcacag gctgtcaatc tacgaggagt gggacccctt ccggtttcgc
2581 cacatgatcc ctaccgaagc tttgcaggtc cgagctctgc ccagtgcaga tgcagaggca
2641 aatgcggtat gcgaaattgt ccacgtgaaa tcagagtcag aagggaggcc cgagcgggtt
2701 ttccacctct gctgcagttc cccagagagc agaaaggact ttctgaagtc tgtgcattcg
2761 atcctgcgag ataaacacag aagacagtaa ggtaccgcgg gcccgggatc caccggatct
2821 agataactga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
2881 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
2941 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
3001 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc
3061 gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
3121 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
3181 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
3241 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
3301 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
3361 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
3421 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
3481 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa
3541 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
3601 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga
3661 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
3721 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
3781 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
3841 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
3901 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
3961 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga
4021 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
4081 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
4141 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
4201 gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac
4261 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
4321 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
4381 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
4441 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
4501 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
4561 gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
4621 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
4681 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
4741 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
4801 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
4861 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
4921 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
4981 gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag
5041 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt
5101 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
5161 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
5221 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
5281 gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
5341 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
5401 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
5461 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
5521 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
5581 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
5641 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
5701 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
5761 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
5821 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
5881 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
5941 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
6001 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
6061 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
6121 gtggataacc gtattaccgc catgcat
//
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