质粒名称: | Ai9 |
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出品公司: | 爱迪基因 |
目录编号: | 22799 |
存储实验室: | Hongkui Zeng |
载体骨架基本信息 |
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载体名称: | Rosa26 Targeting Vector |
载体出品公司: | -- |
载体抗性: | 氨苄青霉素 |
空载体大小: | -- |
完整质粒大小: | 9862 bp |
载体修饰: | -- |
载体类型: | 哺乳细胞表达 |
筛选标记: | 新霉素(G418) |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | CAG-Floxed tdTomato |
物种来源: | -- |
插入大小: | 7057 bp |
基因突变: | -- |
GenBank编号 | -- |
启动子 | -- |
标签/融合蛋白: | 人细胞色素c氧化酶亚基VIII(N端) |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | AgeI(未破坏) |
3' 克隆位点: | NotI (未破坏) |
5' 测序引物 | CMV-F |
3' 测序引物 | SV40pA-R |
文献引用方法 |
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材料和方法部分: | Ai9 was a gift from Hongkui Zeng (Addgene plasmid # 22799 ; http://n2t.net/addgene:22799 ; RRID:Addgene_22799) |
参考文献部分: | A robust and high-throughput Cre reporting and characterization system for the whole mouse brain. Madisen L, Zwingman TA, Sunkin SM, Oh SW, Zariwala HA, Gu H, Ng LL, Palmiter RD, Hawrylycz MJ, Jones AR, Lein ES, Zeng H. Nat Neurosci. 2010 Jan . 13(1):133-40. 10.1038/nn.2467 PubMed 20023653 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS Ai9 17021 bp ds-DNA circular SYN 06-AUG-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Ai9 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 17021) AUTHORS Madisen L, Zwingman TA, Sunkin SM, Oh SW, Zariwala HA, Gu H, Ng LL, Palmiter RD, Hawrylycz MJ, Jones AR, Lein ES, Zeng H TITLE A robust and high-throughput Cre reporting and characterization system for the whole mouse brain. JOURNAL Nat Neurosci. 2010 Jan . 13(1):133-40. PUBMED 20023653 REFERENCE 2 (bases 1 to 17021) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..17021 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 3..1085 /label=Rosa26 left arm /note="left homology arm from intron 1 of the mouse Rosa26 gene (Soriano, 1999)" enhancer 1181..1484 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 1486..1762 /label=chicken beta-actin promoter intron 1769..2785 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" primer_bind 2709..2731 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 2793..2812 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" protein_bind 2861..2894 /label=FRT (minimal) /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="supports FLP-mediated excision but not integration (Turan and Bode, 2011)" protein_bind complement(2918..2951) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." polyA_signal 3118..3252 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" polyA_signal 3372..3506 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" polyA_signal 3626..3760 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" protein_bind complement(3789..3822) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." regulatory 3897..3906 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3903..5333 /codon_start=1 /product="tandem dimeric (pseudo-monomeric) derivative of DsRed (Shaner et al., 2004)" /label=tdTomato /note="mammalian codon-optimized" /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE RSEGRHHLFLYGMDELYK" misc_feature 5399..5987 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5452..5472) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(5870..5881) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(5990..6006) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5991..6007) /label=pBluescriptKS /note="For pBluescript vector" polyA_signal 6014..6221 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" protein_bind 6229..6262 /label=attB /bound_moiety="phage phi-C31 integrase" /note="minimal attB site for the phi-C31 integrase (Groth et al., 2000)" promoter 6284..6783 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" protein_bind 6803..6836 /label=FRT (minimal) /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="supports FLP-mediated excision but not integration (Turan and Bode, 2011)" regulatory 6837..6846 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 6843..7646 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(6906..6925) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 7516..7535 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 7656..8117 /label=PGK poly(A) signal /note="mouse phosphoglycerate kinase 1 polyadenylation signal" misc_feature 8183..12523 /label=Rosa26 right arm /note="right homology arm from intron 1 of the mouse Rosa26 gene (Soriano, 1999)" promoter 12573..13072 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" CDS 13099..13671 /codon_start=1 /gene="Corynebacterium diphtheriae tox" /product="diphtheria toxin fragment A" /label=DTA /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI NFETRGKRGQDAMYEYMAQACAGNRVRR" intron 13748..13813 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" polyA_signal 13887..14111 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind complement(14173..14193) /label=T3 /note="T3 promoter, forward primer" promoter complement(14173..14191) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(14212..14228) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(14212..14228) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(14225..14247) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 14236..14252 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(14260..14290) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 14305..14326 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(14443..14460) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(14614..15202) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(14694..14713) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(15373..16233) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 15996..16015 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(16234..16338) /gene="bla" /label=AmpR promoter rep_origin complement(16364..16819) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(16501..16522) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 16713..16732 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 16946..16968 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 16960..16977 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 16961..16977 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 16987..17006 /label=T7 /note="T7 promoter, forward primer" promoter 16987..17005 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" ORIGIN 1 ccgcggcagg ccctccgagc gtggtggagc cgttctgtga gacagccggg tacgagtcgt 61 gacgctggaa ggggcaagcg ggtggtgggc aggaatgcgg tccgccctgc agcaaccgga 121 gggggaggga gaagggagcg gaaaagtctc caccggacgc ggccatggct cggggggggg 181 ggggcagcgg aggagcgctt ccggccgacg tctcgtcgct gattggcttc ttttcctccc 241 gccgtgtgtg aaaacacaaa tggcgtgttt tggttggcgt aaggcgcctg tcagttaacg 301 gcagccggag tgcgcagccg ccggcagcct cgctctgccc actgggtggg gcgggaggta 361 ggtggggtga ggcgagctgg acgtgcgggc gcggtcggcc tctggcgggg cggggggggg 421 gggggggggt cagcgaaagt agctcgcgcg cgagcggccg cccaccctcc ccttcctctg 481 ggggagtcgt tttacccgcc gccggccggg cctcgtcgtc tgattggctc tcggggccca 541 gaaaactggc ccttgccatt ggctcgtgtt cgtgcaagtt gagtccatcc gccggccagc 601 gggggcggcg aggaggcgct cccaggttcc ggccctcccc tcggccccgc gccgcagagt 661 ctggccgcgc gcccctgcgc aacgtggcag gaagcgcgcg ctgggggcgg ggacgggcag 721 tagggctgag cggctgcggg gcgggtgcaa gcacgtttcc gacttgagtt gcctcaagag 781 gggcgtgctg agccagacct ccatcgcgca ctccggggag tggagggaag gagcgagggc 841 tcagttgggc tgttttggag gcaggaagca cttgctctcc caaagtcgct ctgagttgtt 901 atcagtaagg gagctgcagt ggagtaggcg gggagaaggc cgcacccttc tccggagggg 961 ggaggggagt gttgcaatac ctttctggga gttctctgct gcctcctggc ttctgaggac 1021 cgccctgggc ctgggagaat cccttccccc tcttccctcg tgatctgcaa ctccagtctt 1081 tctagcctta attaaccgtt taaacaattc tgcaggaatc tagttattaa tagtaatcaa 1141 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa 1201 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 1261 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 1321 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 1381 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 1441 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca 1501 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta 1561 ttttttaatt attttgtgca gcgatggggg cggggggggg ggggggsgcg cgccaggcgg 1621 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca 1681 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa 1741 aaagcgaagc gcgcggcggg cgcgggcggg agtcgctgcg cgctgccttc gccccgtgcc 1801 ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca 1861 ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg 1921 gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg 1981 cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg 2041 ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg 2101 cagtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga 2161 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc 2221 gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc 2281 ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg 2341 gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga 2401 ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg 2461 ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctgtgcgga 2521 gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga agcggtgcgg 2581 cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct 2641 tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg 2701 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt 2761 tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca 2821 tcattttggc aaagaattga tttgataccg cgggccctaa gaagttccta ttctctagaa 2881 agtataggaa cttcgtcgac atttaaatca tttaaatata acttcgtata atgtatgcta 2941 tacgaagtta ttcgcgatga ataaatgaaa gcttgcagat ctgcgactct agaggatctg 3001 cgactctaga ggatcataat cagccatacc acatttgtag aggttttact tgctttaaaa 3061 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac 3121 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 3181 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 3241 catgtctgga tctgcgactc tagaggatca taatcagcca taccacattt gtagaggttt 3301 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa 3361 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 3421 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 3481 tcaatgtatc ttatcatgtc tggatctgcg actctagagg atcataatca gccataccac 3541 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca 3601 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata 3661 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 3721 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cccatcaagc tgatccggaa 3781 cccttaatat aacttcgtat aatgtatgct atacgaagtt attaggtccc tcgacctgca 3841 gcccaagcta gatcgaattc ggccggcctt gtacgcgtta agtgcaacac gatcccgcca 3901 ccatggtgag caagggcgag gaggtcatca aagagttcat gcgcttcaag gtgcgcatgg 3961 agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg 4021 agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg 4081 acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca 4141 tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact 4201 tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga 4261 tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga 4321 agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg 4381 gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga 4441 ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc 4501 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg 4561 gccgccacca cctgttcctg gggcatggca ccggcagcac cggcagcggc agctccggca 4621 ccgcctcctc cgaggacaac aacatggccg tcatcaaaga gttcatgcgc ttcaaggtgc 4681 gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc 4741 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg 4801 cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg 4861 ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga 4921 tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg caggacggca 4981 cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc 5041 agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc 5101 tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt 5161 tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca 5221 ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct 5281 ccgagggccg ccaccacctg ttcctgtacg gcatggacga gctgtacaag taagaattgt 5341 gttgcactta acgcgtacaa ggccggccct gcaggaattc gatatcaagc ttatcgataa 5401 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc 5461 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat 5521 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg 5581 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg 5641 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat 5701 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt 5761 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc 5821 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa 5881 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg 5941 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg 6001 acctcgacct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg 6061 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt 6121 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc 6181 aagggggagg attgggaaga caatggcagg catgctgggg aactagtggt gccagggcgt 6241 gcccttgggc tccccgggcg cggcggccgc atcgaattct accgggtagg ggaggcgctt 6301 ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca 6361 agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc 6421 tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc 6481 ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg 6541 cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag 6601 cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg 6661 ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc 6721 tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt 6781 tcgacctgca atcgccgcta gcgaagttcc tattctctag aaagtatagg aacttcgcca 6841 ccatgggatc ggccattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg 6901 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt 6961 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc 7021 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt 7081 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag 7141 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg 7201 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag 7261 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg 7321 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc 7381 gcatgcccga cggcgatgat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca 7441 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc 7501 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg 7561 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct 7621 atcgccttct tgacgagttc ttctgagggg atccgctgta agtctgcaga aattgatgat 7681 ctattaaaca ataaagatgt ccactaaaat ggaagttttt cctgtcatac tttgttaaga 7741 agggtgagaa cagagtacct acattttgaa tggaaggatt ggagctacgg gggtgggggt 7801 ggggtgggat tagataaatg cctgctcttt actgaaggct ctttactatt gctttatgat 7861 aatgtttcat agttggatat cataatttaa acaagcaaaa ccaaattaag ggccagctca 7921 ttcctcccac tcatgatcta tagatctata gatctctcgt gggatcattg tttttctctt 7981 gattcccact ttgtggttct aagtactgtg gtttccaaat gtgtcagttt catagcctga 8041 agaacgagat cagcagcctc tgttccacat acacttcatt ctcagtattg ttttgccaag 8101 ttctaattcc atcagaaagc ttgcagaaga tctccccaac tggggtaacc tttgagttct 8161 ctcagttggg ggggcgcgcc ggctagaaga tgggcgggag tcttctgggc aggcttaaag 8221 gctaacctgg tgtgtgggcg ttgtcctgca ggggaattga acaggtgtaa aattggaggg 8281 acaagacttc ccacagattt tcggttttgt cgggaagttt tttaataggg gcaaataagg 8341 aaaatgggag gataggtagt catctggggt tttatgcagc aaaactacag gttattattg 8401 cttgtgatcc gcctcggagt attttccatc gaggtagatt aaagacatgc tcacccgagt 8461 tttatactct cctgcttgag atccttacta cagtatgaaa ttacagtgtc gcgagttaga 8521 ctatgtaagc agaattttaa tcatttttaa agagcccagt acttcatatc catttctccc 8581 gctccttctg cagccttatc aaaaggtatt ttagaacact cattttagcc ccattttcat 8641 ttattatact ggcttatcca acccctagac agagcattgg cattttccct ttcctgatct 8701 tagaagtctg atgactcatg aaaccagaca gattagttac atacaccaca aatcgaggct 8761 gtagctgggg cctcaacact gcagttcttt tataactcct tagtacactt tttgttgatc 8821 ctttgccttg atccttaatt ttcagtgtct atcacctctc ccgtcaggtg gtgttccaca 8881 tttgggccta ttctcagtcc agggagtttt acaacaatag atgtattgag aatccaacct 8941 aaagcttaac tttccactcc catgaatgcc tctctccttt ttctccattt ataaactgag 9001 ctattaacca ttaatggttt ccaggtggat gtctcctccc ccaatattac ctgatgtatc 9061 ttacatattg ccaggctgat attttaagac attaaaaggt atatttcatt attgagccac 9121 atggtattga ttactgctta ctaaaatttt gtcattgtac acatctgtaa aaggtggttc 9181 cttttggaat gcaaagttca ggtgtttgtt gtctttcctg acctaaggtc ttgtgagctt 9241 gtattttttc tatttaagca gtgctttctc ttggactggc ttgactcatg gcattctaca 9301 cgttattgct ggtctaaatg tgattttgcc aagcttcttc aggacctata attttgcttg 9361 acttgtagcc aaacacaagt aaaatgatta agcaacaaat gtatttgtga agcttggttt 9421 ttaggttgtt gtgttgtgtg tgcttgtgct ctataataat actatccagg ggctggagag 9481 gtggctcgga gttcaagagc acagactgct cttccagaag tcctgagttc aattcccagc 9541 aaccacatgg tggctcacaa ccatctgtaa tgggatctga tgccctcttc tggtgtgtct 9601 gaagaccaca agtgtattca cattaaataa ataaatcctc cttcttcttc tttttttttt 9661 ttttaaagag aatactgtct ccagtagaat ttactgaagt aatgaaatac tttgtgtttg 9721 ttccaatatg gtagccaata atcaaattac tctttaagca ctggaaatgt taccaaggaa 9781 ctaattttta tttgaagtgt aactgtggac agaggagcca taactgcaga cttgtgggat 9841 acagaagacc aatgcagact ttaatgtctt ttctcttaca ctaagcaata aagaaataaa 9901 aattgaactt ctagtatcct atttgtttaa actgctagct ttacttaact tttgtgcttc 9961 atctatacaa agctgaaagc taagtctgca gccattacta aacatgaaag caagtaatga 10021 taattttgga tttcaaaaat gtagggccag agtttagcca gccagtggtg gtgcttgcct 10081 ttatgccttt aatcccagca ctctggaggc agagacaggc agatctctga gtttgagccc 10141 agcctggtct acacatcaag ttctatctag gatagccagg aatacacaca gaaaccctgt 10201 tggggagggg ggctctgaga tttcataaaa ttataattga agcattccct aatgagccac 10261 tatggatgtg gctaaatccg tctacctttc tgatgagatt tgggtattat tttttctgtc 10321 tctgctgttg gttgggtctt ttgacactgt gggctttctt taaagcctcc ttcctgccat 10381 gtggtctctt gtttgctact aacttcccat ggcttaaatg gcatggcttt ttgccttcta 10441 agggcagctg ctgagatttg cagcctgatt tccagggtgg ggttgggaaa tctttcaaac 10501 actaaaattg tcctttaatt ttttttttaa aaaatgggtt atataataaa cctcataaaa 10561 tagttatgag gagtgaggtg gactaatatt aaatgagtcc ctcccctata aaagagctat 10621 taaggctttt tgtcttatac ttaacttttt ttttaaatgt ggtatcttta gaaccaaggg 10681 tcttagagtt ttagtataca gaaactgttg catcgcttaa tcagattttc tagtttcaaa 10741 tccagagaat ccaaattctt cacagccaaa gtcaaattaa gaatttctga cttttaatgt 10801 taatttgctt actgtgaata taaaaatgat agcttttcct gaggcagggt ctcactatgt 10861 atctctgcct gatctgcaac aagatatgta gactaaagtt ctgcctgctt ttgtctcctg 10921 aatactaagg ttaaaatgta gtaatacttt tggaacttgc aggtcagatt cttttatagg 10981 ggacacacta agggagcttg ggtgatagtt ggtaaaatgt gtttcaagtg atgaaaactt 11041 gaattattat caccgcaacc tactttttaa aaaaaaaagc caggcctgtt agagcatgct 11101 taagggatcc ctaggacttg ctgagcacac aagagtagtt acttggcagg ctcctggtga 11161 gagcatattt caaaaaacaa ggcagacaac caagaaacta cagttaaggt tacctgtctt 11221 taaaccatct gcatatacac agggatatta aaatattcca aataatattt cattcaagtt 11281 ttcccccatc aaattgggac atggatttct ccggtgaata ggcagagttg gaaactaaac 11341 aaatgttggt tttgtgattt gtgaaattgt tttcaagtga tagttaaagc ccatgagata 11401 cagaacaaag ctgctatttc gaggtctctt ggtttatact cagaagcact tctttgggtt 11461 tccctgcact atcctgatca tgtgctaggc ctaccttagg ctgattgttg ttcaaataaa 11521 cttaagtttc ctgtcaggtg atgtcatatg atttcatata tcaaggcaaa acatgttata 11581 tatgttaaac atttgtactt aatgtgaaag ttaggtcttt gtgggtttga tttttaattt 11641 tcaaaacctg agctaaataa gtcattttta catgtcttac atttggtgga attgtataat 11701 tgtggtttgc aggcaagact ctctgaccta gtaaccctac ctatagagca ctttgctggg 11761 tcacaagtct aggagtcaag catttcacct tgaagttgag acgttttgtt agtgtatact 11821 agtttatatg ttggaggaca tgtttatcca gaagatattc aggactattt ttgactgggc 11881 taaggaattg attctgatta gcactgttag tgagcattga gtggccttta ggcttgaatt 11941 ggagtcactt gtatatctca aataatgctg gcctttttta aaaagccctt gttctttatc 12001 accctgtttt ctacataatt tttgttcaaa gaaatacttg tttggatctc cttttgacaa 12061 caatagcatg ttttcaagcc atattttttt tccttttttt tttttttttt ggtttttcga 12121 gacagggttt ctctgtatag ccctggctgt cctggaactc actttgtaga ccaggctggc 12181 ctcgaactca gaaatccgcc tgcctctgcc tcctgagtgc cgggattaaa ggcgtgcacc 12241 accacgcctg gctaagttgg atattttgtt atataactat aaccaatact aactccactg 12301 ggtggatttt taattcagtc agtagtctta agtggtcttt attggccctt cattaaaatc 12361 tactgttcac tctaacagag gctgttggta ctagtggcac ttaagcaact tcctacggat 12421 atactagcag attaagggtc agggatagaa actagtctag cgttttgtat acctaccagc 12481 tttatactac cttgttctga tagaaatatt tcaggacatc tagcttatcg ataccgtcga 12541 cggtatcgat aagcttgata tcgaattcta ccgggtaggg gaggcgcttt tcccaaggca 12601 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg 12661 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc 12721 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc 12781 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag 12841 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc 12901 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg 12961 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca 13021 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag 13081 gtcctcgcca tggatcctga tgatgttgtt gattcttcta aatcttttgt gatggaaaac 13141 ttttcttcgt accacgggac taaacctggt tatgtagatt ccattcaaaa aggtatacaa 13201 aagccaaaat ctggtacaca aggaaattat gacgatgatt ggaaagggtt ttatagtacc 13261 gacaataaat acgacgctgc gggatactct gtagataatg aaaacccgct ctctggaaaa 13321 gctggaggcg tggtcaaagt gacgtatcca ggactgacga aggttctcgc actaaaagtg 13381 gataatgccg aaactattaa gaaagagtta ggtttaagtc tcactgaacc gttgatggag 13441 caagtcggaa cggaagagtt tatcaaaagg ttcggtgatg gtgcttcgcg tgtagtgctc 13501 agccttccct tcgctgaggg gagttctagc gttgaatata ttaataactg ggaacaggcg 13561 aaagcgttaa gcgtagaact tgagattaat tttgaaaccc gtggaaaacg tggccaagat 13621 gcgatgtatg agtatatggc tcaagcctgt gcaggaaatc gtgtcaggcg atctctttgt 13681 gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag 13741 ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt 13801 ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcaga 13861 tcctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg 13921 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata 13981 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt 14041 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt 14101 gggctctatg gcttctgagg cggaaagaac cagctggggc tcgagggggg gcccggtacc 14161 cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct 14221 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 14281 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 14341 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 14401 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 14461 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 14521 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 14581 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 14641 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 14701 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 14761 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 14821 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 14881 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 14941 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 15001 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt 15061 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 15121 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 15181 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 15241 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 15301 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 15361 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 15421 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 15481 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 15541 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 15601 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 15661 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 15721 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 15781 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 15841 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 15901 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 15961 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 16021 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 16081 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 16141 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 16201 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 16261 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 16321 acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat 16381 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc 16441 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt 16501 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa 16561 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg 16621 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga 16681 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct 16741 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat 16801 gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga 16861 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga 16921 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag 16981 cgcgcgtaat acgactcact atagggcgaa ttggagctcc a //
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