Ai9

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联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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载体基本信息

质粒名称: Ai9 
出品公司: 爱迪基因
目录编号: 22799
存储实验室: Hongkui Zeng

载体骨架基本信息
载体名称: Rosa26 Targeting Vector
载体出品公司: --
载体抗性: 氨苄青霉素
空载体大小: --
完整质粒大小: 9862 bp
载体修饰: --
载体类型: 哺乳细胞表达
筛选标记: 新霉素G418
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: CAG-Floxed tdTomato
物种来源: --
插入大小: 7057 bp
基因突变: --
GenBank编号 --
启动子 --
标签/融合蛋白: 人细胞色素c氧化酶亚基VIII(N端)
克隆方法: 限制性内切酶
5' 克隆位点: AgeI(未破坏)
3' 克隆位点: NotI (未破坏)
5' 测序引物 CMV-F
3' 测序引物 SV40pA-R

文献引用方法
材料和方法部分: Ai9 was a gift from Hongkui Zeng (Addgene plasmid # 22799 ; http://n2t.net/addgene:22799 ; RRID:Addgene_22799)
参考文献部分: A robust and high-throughput Cre reporting and characterization system for the whole mouse brain. Madisen L, Zwingman TA, Sunkin SM, Oh SW, Zariwala HA, Gu H, Ng LL, Palmiter RD, Hawrylycz MJ, Jones AR, Lein ES, Zeng H. Nat Neurosci. 2010 Jan . 13(1):133-40. 10.1038/nn.2467 PubMed 20023653

载体图谱质粒图谱和多克隆位点信息

Ai9质粒图谱

载体简介

载体序列

LOCUS       Ai9               17021 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Ai9
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17021)
  AUTHORS   Madisen L, Zwingman TA, Sunkin SM, Oh SW, Zariwala HA, Gu H, Ng LL, 
            Palmiter RD, Hawrylycz MJ, Jones AR, Lein ES, Zeng H
  TITLE     A robust and high-throughput Cre reporting and characterization 
            system for the whole mouse brain.
  JOURNAL   Nat Neurosci. 2010 Jan . 13(1):133-40.
  PUBMED    20023653
REFERENCE   2  (bases 1 to 17021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..17021
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    3..1085
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     enhancer        1181..1484
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1486..1762
                     /label=chicken beta-actin promoter
     intron          1769..2785
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2709..2731
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2793..2812
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    2861..2894
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(2918..2951)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     polyA_signal    3118..3252
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    3372..3506
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    3626..3760
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3789..3822)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     regulatory      3897..3906
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3903..5333
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    5399..5987
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5452..5472)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5870..5881)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5990..6006)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5991..6007)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    6014..6221
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    6229..6262
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        6284..6783
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     protein_bind    6803..6836
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      6837..6846
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             6843..7646
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6906..6925)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7516..7535
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7656..8117
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     misc_feature    8183..12523
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     promoter        12573..13072
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             13099..13671
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          13748..13813
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    13887..14111
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(14173..14193)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(14173..14191)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(14212..14228)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14212..14228)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14225..14247)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14236..14252
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(14260..14290)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14305..14326
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(14443..14460)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14614..15202)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14694..14713)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(15373..16233)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15996..16015
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16234..16338)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(16364..16819)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(16501..16522)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     16713..16732
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     16946..16968
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     16960..16977
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     16961..16977
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     16987..17006
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        16987..17005
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccgcggcagg ccctccgagc gtggtggagc cgttctgtga gacagccggg tacgagtcgt
       61 gacgctggaa ggggcaagcg ggtggtgggc aggaatgcgg tccgccctgc agcaaccgga
      121 gggggaggga gaagggagcg gaaaagtctc caccggacgc ggccatggct cggggggggg
      181 ggggcagcgg aggagcgctt ccggccgacg tctcgtcgct gattggcttc ttttcctccc
      241 gccgtgtgtg aaaacacaaa tggcgtgttt tggttggcgt aaggcgcctg tcagttaacg
      301 gcagccggag tgcgcagccg ccggcagcct cgctctgccc actgggtggg gcgggaggta
      361 ggtggggtga ggcgagctgg acgtgcgggc gcggtcggcc tctggcgggg cggggggggg
      421 gggggggggt cagcgaaagt agctcgcgcg cgagcggccg cccaccctcc ccttcctctg
      481 ggggagtcgt tttacccgcc gccggccggg cctcgtcgtc tgattggctc tcggggccca
      541 gaaaactggc ccttgccatt ggctcgtgtt cgtgcaagtt gagtccatcc gccggccagc
      601 gggggcggcg aggaggcgct cccaggttcc ggccctcccc tcggccccgc gccgcagagt
      661 ctggccgcgc gcccctgcgc aacgtggcag gaagcgcgcg ctgggggcgg ggacgggcag
      721 tagggctgag cggctgcggg gcgggtgcaa gcacgtttcc gacttgagtt gcctcaagag
      781 gggcgtgctg agccagacct ccatcgcgca ctccggggag tggagggaag gagcgagggc
      841 tcagttgggc tgttttggag gcaggaagca cttgctctcc caaagtcgct ctgagttgtt
      901 atcagtaagg gagctgcagt ggagtaggcg gggagaaggc cgcacccttc tccggagggg
      961 ggaggggagt gttgcaatac ctttctggga gttctctgct gcctcctggc ttctgaggac
     1021 cgccctgggc ctgggagaat cccttccccc tcttccctcg tgatctgcaa ctccagtctt
     1081 tctagcctta attaaccgtt taaacaattc tgcaggaatc tagttattaa tagtaatcaa
     1141 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     1201 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     1261 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     1321 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     1381 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     1441 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
     1501 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
     1561 ttttttaatt attttgtgca gcgatggggg cggggggggg ggggggsgcg cgccaggcgg
     1621 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca
     1681 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa
     1741 aaagcgaagc gcgcggcggg cgcgggcggg agtcgctgcg cgctgccttc gccccgtgcc
     1801 ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca
     1861 ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg
     1921 gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg
     1981 cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg
     2041 ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg
     2101 cagtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga
     2161 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc
     2221 gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc
     2281 ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg
     2341 gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga
     2401 ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg
     2461 ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctgtgcgga
     2521 gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga agcggtgcgg
     2581 cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct
     2641 tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg
     2701 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt
     2761 tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca
     2821 tcattttggc aaagaattga tttgataccg cgggccctaa gaagttccta ttctctagaa
     2881 agtataggaa cttcgtcgac atttaaatca tttaaatata acttcgtata atgtatgcta
     2941 tacgaagtta ttcgcgatga ataaatgaaa gcttgcagat ctgcgactct agaggatctg
     3001 cgactctaga ggatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     3061 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     3121 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     3181 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     3241 catgtctgga tctgcgactc tagaggatca taatcagcca taccacattt gtagaggttt
     3301 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     3361 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     3421 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     3481 tcaatgtatc ttatcatgtc tggatctgcg actctagagg atcataatca gccataccac
     3541 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     3601 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     3661 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     3721 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cccatcaagc tgatccggaa
     3781 cccttaatat aacttcgtat aatgtatgct atacgaagtt attaggtccc tcgacctgca
     3841 gcccaagcta gatcgaattc ggccggcctt gtacgcgtta agtgcaacac gatcccgcca
     3901 ccatggtgag caagggcgag gaggtcatca aagagttcat gcgcttcaag gtgcgcatgg
     3961 agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     4021 agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg
     4081 acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca
     4141 tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     4201 tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga
     4261 tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga
     4321 agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg
     4381 gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga
     4441 ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc
     4501 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg
     4561 gccgccacca cctgttcctg gggcatggca ccggcagcac cggcagcggc agctccggca
     4621 ccgcctcctc cgaggacaac aacatggccg tcatcaaaga gttcatgcgc ttcaaggtgc
     4681 gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     4741 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg
     4801 cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg
     4861 ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     4921 tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg caggacggca
     4981 cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc
     5041 agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc
     5101 tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt
     5161 tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca
     5221 ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct
     5281 ccgagggccg ccaccacctg ttcctgtacg gcatggacga gctgtacaag taagaattgt
     5341 gttgcactta acgcgtacaa ggccggccct gcaggaattc gatatcaagc ttatcgataa
     5401 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     5461 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     5521 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     5581 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     5641 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     5701 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     5761 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     5821 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     5881 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     5941 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
     6001 acctcgacct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     6061 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     6121 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     6181 aagggggagg attgggaaga caatggcagg catgctgggg aactagtggt gccagggcgt
     6241 gcccttgggc tccccgggcg cggcggccgc atcgaattct accgggtagg ggaggcgctt
     6301 ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca
     6361 agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc
     6421 tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc
     6481 ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg
     6541 cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag
     6601 cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg
     6661 ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc
     6721 tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt
     6781 tcgacctgca atcgccgcta gcgaagttcc tattctctag aaagtatagg aacttcgcca
     6841 ccatgggatc ggccattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     6901 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     6961 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     7021 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     7081 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     7141 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     7201 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     7261 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     7321 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
     7381 gcatgcccga cggcgatgat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     7441 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     7501 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     7561 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     7621 atcgccttct tgacgagttc ttctgagggg atccgctgta agtctgcaga aattgatgat
     7681 ctattaaaca ataaagatgt ccactaaaat ggaagttttt cctgtcatac tttgttaaga
     7741 agggtgagaa cagagtacct acattttgaa tggaaggatt ggagctacgg gggtgggggt
     7801 ggggtgggat tagataaatg cctgctcttt actgaaggct ctttactatt gctttatgat
     7861 aatgtttcat agttggatat cataatttaa acaagcaaaa ccaaattaag ggccagctca
     7921 ttcctcccac tcatgatcta tagatctata gatctctcgt gggatcattg tttttctctt
     7981 gattcccact ttgtggttct aagtactgtg gtttccaaat gtgtcagttt catagcctga
     8041 agaacgagat cagcagcctc tgttccacat acacttcatt ctcagtattg ttttgccaag
     8101 ttctaattcc atcagaaagc ttgcagaaga tctccccaac tggggtaacc tttgagttct
     8161 ctcagttggg ggggcgcgcc ggctagaaga tgggcgggag tcttctgggc aggcttaaag
     8221 gctaacctgg tgtgtgggcg ttgtcctgca ggggaattga acaggtgtaa aattggaggg
     8281 acaagacttc ccacagattt tcggttttgt cgggaagttt tttaataggg gcaaataagg
     8341 aaaatgggag gataggtagt catctggggt tttatgcagc aaaactacag gttattattg
     8401 cttgtgatcc gcctcggagt attttccatc gaggtagatt aaagacatgc tcacccgagt
     8461 tttatactct cctgcttgag atccttacta cagtatgaaa ttacagtgtc gcgagttaga
     8521 ctatgtaagc agaattttaa tcatttttaa agagcccagt acttcatatc catttctccc
     8581 gctccttctg cagccttatc aaaaggtatt ttagaacact cattttagcc ccattttcat
     8641 ttattatact ggcttatcca acccctagac agagcattgg cattttccct ttcctgatct
     8701 tagaagtctg atgactcatg aaaccagaca gattagttac atacaccaca aatcgaggct
     8761 gtagctgggg cctcaacact gcagttcttt tataactcct tagtacactt tttgttgatc
     8821 ctttgccttg atccttaatt ttcagtgtct atcacctctc ccgtcaggtg gtgttccaca
     8881 tttgggccta ttctcagtcc agggagtttt acaacaatag atgtattgag aatccaacct
     8941 aaagcttaac tttccactcc catgaatgcc tctctccttt ttctccattt ataaactgag
     9001 ctattaacca ttaatggttt ccaggtggat gtctcctccc ccaatattac ctgatgtatc
     9061 ttacatattg ccaggctgat attttaagac attaaaaggt atatttcatt attgagccac
     9121 atggtattga ttactgctta ctaaaatttt gtcattgtac acatctgtaa aaggtggttc
     9181 cttttggaat gcaaagttca ggtgtttgtt gtctttcctg acctaaggtc ttgtgagctt
     9241 gtattttttc tatttaagca gtgctttctc ttggactggc ttgactcatg gcattctaca
     9301 cgttattgct ggtctaaatg tgattttgcc aagcttcttc aggacctata attttgcttg
     9361 acttgtagcc aaacacaagt aaaatgatta agcaacaaat gtatttgtga agcttggttt
     9421 ttaggttgtt gtgttgtgtg tgcttgtgct ctataataat actatccagg ggctggagag
     9481 gtggctcgga gttcaagagc acagactgct cttccagaag tcctgagttc aattcccagc
     9541 aaccacatgg tggctcacaa ccatctgtaa tgggatctga tgccctcttc tggtgtgtct
     9601 gaagaccaca agtgtattca cattaaataa ataaatcctc cttcttcttc tttttttttt
     9661 ttttaaagag aatactgtct ccagtagaat ttactgaagt aatgaaatac tttgtgtttg
     9721 ttccaatatg gtagccaata atcaaattac tctttaagca ctggaaatgt taccaaggaa
     9781 ctaattttta tttgaagtgt aactgtggac agaggagcca taactgcaga cttgtgggat
     9841 acagaagacc aatgcagact ttaatgtctt ttctcttaca ctaagcaata aagaaataaa
     9901 aattgaactt ctagtatcct atttgtttaa actgctagct ttacttaact tttgtgcttc
     9961 atctatacaa agctgaaagc taagtctgca gccattacta aacatgaaag caagtaatga
    10021 taattttgga tttcaaaaat gtagggccag agtttagcca gccagtggtg gtgcttgcct
    10081 ttatgccttt aatcccagca ctctggaggc agagacaggc agatctctga gtttgagccc
    10141 agcctggtct acacatcaag ttctatctag gatagccagg aatacacaca gaaaccctgt
    10201 tggggagggg ggctctgaga tttcataaaa ttataattga agcattccct aatgagccac
    10261 tatggatgtg gctaaatccg tctacctttc tgatgagatt tgggtattat tttttctgtc
    10321 tctgctgttg gttgggtctt ttgacactgt gggctttctt taaagcctcc ttcctgccat
    10381 gtggtctctt gtttgctact aacttcccat ggcttaaatg gcatggcttt ttgccttcta
    10441 agggcagctg ctgagatttg cagcctgatt tccagggtgg ggttgggaaa tctttcaaac
    10501 actaaaattg tcctttaatt ttttttttaa aaaatgggtt atataataaa cctcataaaa
    10561 tagttatgag gagtgaggtg gactaatatt aaatgagtcc ctcccctata aaagagctat
    10621 taaggctttt tgtcttatac ttaacttttt ttttaaatgt ggtatcttta gaaccaaggg
    10681 tcttagagtt ttagtataca gaaactgttg catcgcttaa tcagattttc tagtttcaaa
    10741 tccagagaat ccaaattctt cacagccaaa gtcaaattaa gaatttctga cttttaatgt
    10801 taatttgctt actgtgaata taaaaatgat agcttttcct gaggcagggt ctcactatgt
    10861 atctctgcct gatctgcaac aagatatgta gactaaagtt ctgcctgctt ttgtctcctg
    10921 aatactaagg ttaaaatgta gtaatacttt tggaacttgc aggtcagatt cttttatagg
    10981 ggacacacta agggagcttg ggtgatagtt ggtaaaatgt gtttcaagtg atgaaaactt
    11041 gaattattat caccgcaacc tactttttaa aaaaaaaagc caggcctgtt agagcatgct
    11101 taagggatcc ctaggacttg ctgagcacac aagagtagtt acttggcagg ctcctggtga
    11161 gagcatattt caaaaaacaa ggcagacaac caagaaacta cagttaaggt tacctgtctt
    11221 taaaccatct gcatatacac agggatatta aaatattcca aataatattt cattcaagtt
    11281 ttcccccatc aaattgggac atggatttct ccggtgaata ggcagagttg gaaactaaac
    11341 aaatgttggt tttgtgattt gtgaaattgt tttcaagtga tagttaaagc ccatgagata
    11401 cagaacaaag ctgctatttc gaggtctctt ggtttatact cagaagcact tctttgggtt
    11461 tccctgcact atcctgatca tgtgctaggc ctaccttagg ctgattgttg ttcaaataaa
    11521 cttaagtttc ctgtcaggtg atgtcatatg atttcatata tcaaggcaaa acatgttata
    11581 tatgttaaac atttgtactt aatgtgaaag ttaggtcttt gtgggtttga tttttaattt
    11641 tcaaaacctg agctaaataa gtcattttta catgtcttac atttggtgga attgtataat
    11701 tgtggtttgc aggcaagact ctctgaccta gtaaccctac ctatagagca ctttgctggg
    11761 tcacaagtct aggagtcaag catttcacct tgaagttgag acgttttgtt agtgtatact
    11821 agtttatatg ttggaggaca tgtttatcca gaagatattc aggactattt ttgactgggc
    11881 taaggaattg attctgatta gcactgttag tgagcattga gtggccttta ggcttgaatt
    11941 ggagtcactt gtatatctca aataatgctg gcctttttta aaaagccctt gttctttatc
    12001 accctgtttt ctacataatt tttgttcaaa gaaatacttg tttggatctc cttttgacaa
    12061 caatagcatg ttttcaagcc atattttttt tccttttttt tttttttttt ggtttttcga
    12121 gacagggttt ctctgtatag ccctggctgt cctggaactc actttgtaga ccaggctggc
    12181 ctcgaactca gaaatccgcc tgcctctgcc tcctgagtgc cgggattaaa ggcgtgcacc
    12241 accacgcctg gctaagttgg atattttgtt atataactat aaccaatact aactccactg
    12301 ggtggatttt taattcagtc agtagtctta agtggtcttt attggccctt cattaaaatc
    12361 tactgttcac tctaacagag gctgttggta ctagtggcac ttaagcaact tcctacggat
    12421 atactagcag attaagggtc agggatagaa actagtctag cgttttgtat acctaccagc
    12481 tttatactac cttgttctga tagaaatatt tcaggacatc tagcttatcg ataccgtcga
    12541 cggtatcgat aagcttgata tcgaattcta ccgggtaggg gaggcgcttt tcccaaggca
    12601 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
    12661 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
    12721 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
    12781 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag
    12841 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
    12901 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
    12961 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
    13021 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag
    13081 gtcctcgcca tggatcctga tgatgttgtt gattcttcta aatcttttgt gatggaaaac
    13141 ttttcttcgt accacgggac taaacctggt tatgtagatt ccattcaaaa aggtatacaa
    13201 aagccaaaat ctggtacaca aggaaattat gacgatgatt ggaaagggtt ttatagtacc
    13261 gacaataaat acgacgctgc gggatactct gtagataatg aaaacccgct ctctggaaaa
    13321 gctggaggcg tggtcaaagt gacgtatcca ggactgacga aggttctcgc actaaaagtg
    13381 gataatgccg aaactattaa gaaagagtta ggtttaagtc tcactgaacc gttgatggag
    13441 caagtcggaa cggaagagtt tatcaaaagg ttcggtgatg gtgcttcgcg tgtagtgctc
    13501 agccttccct tcgctgaggg gagttctagc gttgaatata ttaataactg ggaacaggcg
    13561 aaagcgttaa gcgtagaact tgagattaat tttgaaaccc gtggaaaacg tggccaagat
    13621 gcgatgtatg agtatatggc tcaagcctgt gcaggaaatc gtgtcaggcg atctctttgt
    13681 gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag
    13741 ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt
    13801 ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcaga
    13861 tcctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
    13921 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
    13981 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
    14041 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
    14101 gggctctatg gcttctgagg cggaaagaac cagctggggc tcgagggggg gcccggtacc
    14161 cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct
    14221 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
    14281 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
    14341 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
    14401 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
    14461 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
    14521 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
    14581 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
    14641 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
    14701 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
    14761 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
    14821 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
    14881 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
    14941 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
    15001 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
    15061 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
    15121 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
    15181 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
    15241 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
    15301 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
    15361 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
    15421 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
    15481 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    15541 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
    15601 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
    15661 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
    15721 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
    15781 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    15841 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    15901 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    15961 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    16021 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    16081 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    16141 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    16201 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    16261 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    16321 acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat
    16381 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
    16441 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
    16501 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
    16561 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
    16621 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
    16681 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
    16741 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
    16801 gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
    16861 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
    16921 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag
    16981 cgcgcgtaat acgactcact atagggcgaa ttggagctcc a
//

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