| 质粒名称: | pJV62 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 26910 |
| 存储实验室: | Graham Hatfull |
|
载体骨架基本信息 |
|
| 载体名称: | pLAM12 |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 6758 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | 卡那霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Che9c gene 61 |
| 物种来源: | 支原体噬菌体 |
| 插入大小: | 1061 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | NdeI(未破坏) |
| 3' 克隆位点: | NheI (未破坏) |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pJV62 was a gift from Graham Hatfull (Addgene plasmid # 26910 ; http://n2t.net/addgene:26910 ; RRID:Addgene_26910) |
| 参考文献部分: | Efficient point mutagenesis in mycobacteria using single-stranded DNA recombineering: characterization of antimycobacterial drug targets. van Kessel JC, Hatfull GF. Mol Microbiol. 2008 Mar . 67(5):1094-107. 10.1111/j.1365-2958.2008.06109.x PubMed 18221264 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pJV62 7794 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pJV62
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7794)
AUTHORS van Kessel JC, Hatfull GF
TITLE Efficient point mutagenesis in mycobacteria using single-stranded
DNA recombineering: characterization of antimycobacterial drug
targets.
JOURNAL Mol Microbiol. 2008 Mar . 67(5):1094-107.
PUBMED 18221264
REFERENCE 2 (bases 1 to 7794)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7794
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 5700..5746
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
primer_bind complement(5896..5915)
/label=pENTR-R
/note="pENTR vectors, reverse primer"
CDS 5907..6722
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind complement(5980..5999)
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
rep_origin 7053..7641
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7542..7561
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 aacgcgtgag cccaccagct ccgtaagttc gggcgctgtg tggctcgtac ccgcgcattc
61 aggcggcagg gggtctaacg ggtctaaggc ggcgtgtacg gccgccacag cggctctcag
121 cggcccggaa acgtcctcga aacgacgcat gtgttcctcc tggttggtac aggtggttgg
181 gggtgctcgg ctgtcgctgg tgttccacca ccagggctcg acgggagagc gggggagtgt
241 gcagttgtgg ggtggcccct cagcgaaata tctgacttgg agctcgtgtc ggaccataca
301 ccggtgatta atcgtggtct actaccaagc gtgagccacg tcgccgacga atttgagcag
361 ctctggctgc cgtactggcc gctggcaagc gacgatctgc tcgaggggat ctaccgccaa
421 agccgcgcgt cggccctagg ccgccggtac atcgaggcga acccaacagc gctggcaaac
481 ctgctggtcg tggacgtaga ccatccagac gcagcgctcc gagcgctcag cgcccggggg
541 tcccatccgc tgcccaacgc gatcgtgggc aatcgcgcca acggccacgc acacgcagtg
601 tgggcactca acgcccctgt tccacgcacc gaatacgcgc ggcgtaagcc gctcgcatac
661 atggcggcgt gcgccgaagg ccttcggcgc gccgtcgatg gcgaccgcag ttactcaggc
721 ctcatgacca aaaaccccgg ccacatcgcc tgggaaacgg aatggctcca ctcagatctc
781 tacacactca gccacatcga ggccgagctc ggcgcgaaca tgccaccgcc gcgctggcgt
841 cagcagacca cgtacaaagc ggctccgacg ccgctagggc ggaattgcgc actgttcgat
901 tccgtcaggt tgtgggccta tcgtcccgcc ctcatgcgga tctacctgcc gacccggaac
961 gtggacggac tcggccgcgc gatctatgcc gagtgccacg cgcgaaacgc cgaatttccg
1021 tgcaacgacg tgtgtcccgg accgctaccg gacagcgagg tccgcgccat cgccaacagc
1081 atttggcgtt ggatcacaac caagtcgcgc atttgggcgg acgggatcgt ggtctacgag
1141 gccacactca gtgcgcgcca gtcggccatc tcgcggaagg gcgcagcagc gcgcacggcg
1201 gcgagcacag ttgcgcggcg cgcaaagtcc gcgtcagcca tggaggcatt gctatgagcg
1261 acggctacag cgacggctac aaccggcagc cgactgtccg caaaaagcgg cgcgtgaccg
1321 ccgccgaagg cgctcgaatc accggactat ccgaacgcca cgtcgtccgg ctcgtggcgc
1381 aggaacgcag cgagtggctc gccgagcagg ctgcacgccg cgaacgcatc cgcgcctatc
1441 acgacgacga gggccactct tggccgcaaa cggccaaaca tttcgggctg catctggaca
1501 ccgttaagcg actcggctat cgggcgagga aagagcgtgc ggcagaacag gaagcggctc
1561 aaaaggccca caacgaagcc gacaatccac cgctgttcta acgcaattgg ggagcgggtg
1621 tcgcgggggt tccgtggggg gttccgttgc aacgggtcgg acaggtaaaa gtcctggtag
1681 acgctagttt tctggtttgg gccatgcctg tctcgttgcg tgtttcgttg cgcccgtttt
1741 gaataccagc cagacgagac ggggttctac gaatcttggt cgataccaag ccatttccgc
1801 tgaatatcgg ggagctcacc gccagaatcg gtggttgtgg tgatgtacgt ggcgaactcc
1861 gttgtagtgc ctgtggtggc atccgtggcg cggccgcggt accagatctt taaatctaga
1921 taaagaagtg acgcggtctc aagcgtcgag cgtcgccagc gtgtcgagga tgtcgaagtc
1981 gtagccgtcg gcgctggcga tgtagacctg ctggtcgaat tgactgtcgc gcatacacat
2041 cgggccccgg ggcccgtcga acccgacatc gtgcgcggat gccatcaggt ccggtatctc
2101 gggggagtgg gcccgctgga agatggcctc gagcgcaagc agaccctcgt aacaggattc
2161 ggccatcgcg ttgagcggtg gcgcgtcggc gccgtagcgg gcgacgtagc tgcccatcag
2221 gtccatggca cccgcggtgg ccagtgaact gaagtacgcc gcggcgacat agaggttttc
2281 ggtggagccg gcgccgctgg ccagcagcat gttctcctcc atcagcgggc tgaaccgcgc
2341 catgcggtcg tgcccgccgg cgcgcgcgaa ctcgcggttg aacaacacgg cgtcctggcc
2401 gacgagcagc atcaacacgg cctgcgcccc cgacgcgatg gccttgcgga caggtgcgcg
2461 gaaatcgtcg gtgccgtacg ggacgtagat ctcccgtctg agctcgaggt ccagatctcg
2521 gcagtacgcg cgggcggccg cggcggaacg gcgcggccag atgtagtcat cgccgaccag
2581 gcaccaggac cggatgccga agtggtcgcg cagccaggcg agcgcgggcg cgatctggat
2641 ctgcggtgtc tcgcctgtgc agaacacgcc cggtgtgcgt tcaccgccct cgtacaacga
2701 ggtgtagacg tacgggatgc ggtcgcggac caccggggag atgcggttgc gcacggccga
2761 gatgtgccag ccggtcacgg cgtcgagacc gtgacctcgc aaccggtcgg cgacggtccg
2821 ggcgacgtcg tcgccgggcg ctccgccgtc gagcacctcg atggtgacct tgcggccctg
2881 caggccgcct cggtcgttga cctccttggc cgcgagctcg gccacggcct cgcacgaagg
2941 cgcgaagatt cccgctggcc cttgaagcgg aatcaccagc ccgacgcgga actcaacctc
3001 gccgtcctgc actccagatc accgtcgatc ccgtgtagtc tgcgcttcaa agctttctag
3061 cagaaataat tcattctgaa cagaccccgc cgtcgacacg aggagacacc caccatggcc
3121 gccggacagc agcgccgccc caacctcctg ctgccgttgg tgcgtctgac ccacctcgcg
3181 gagtcggcga tcgaacgcgt gctcgcggac tcgtcgctca agatcgagga ctggcgcgtg
3241 ctcgacgagt tggccggacg gcgcaccgtg cccatgagcg atctcgcgca ggccacgctg
3301 atcacgggtc cgactctcac cagaaccgtc gatcgccttg tgtcgcaagg gatcatctac
3361 cggactgccg atctgcatga ccgccggcgg gtgctcgtgg cgttgacccc gcgggggcgg
3421 acgctgcgca accgcctggt ggacgcggta gccgaggccg agtgtgcggc ttttgaatcg
3481 tgcgggctgg acgtcgacca gttgcgcgaa ctcgtcgaca ccacctcgaa tttgacttcg
3541 taaccacccg cgcccggcgc gggcgttcac ccttgacttt tattttcatc tggatatatt
3601 tcgggtgaat ggaaaggggt gaccatgccg acctacacat tccgttgttc ccactgcggt
3661 cccttcgatc tcacctgcgc gatctccgag cgcgatgcgg cggcgacctg tccggagtgc
3721 cggacgccgg cgcgccgggt cttcggttcg gtagggctga cgacattcac cgcgggacat
3781 caccgcgcat tcgacgcggc gtccgcgagc gccgaaagtc ccacggtggt gaagtcgatt
3841 cccgcaggcg cggaccgccc gcgggccccg cgccgcaatc ccggtctacc gagtctgccg
3901 aggtactagc gacatgggtg gcgtcgggct cttctacgtg ggtgcggtgc tcatcatcga
3961 cgggctgatg ctgctgggcc gcatcagccc acgaggcgca acaccgctga acttcttcgt
4021 cggcggactg caggtggtga cgcctacggt gctgatcctg cagtccggcg gagacgcggc
4081 cgtgatcttc gcggcctccg ggctctacct gttcggcttc acctacctgt gggtggccat
4141 caacaacgtg accgactggg acggagaagg tctcggatgg ttctcgctgt tcgtcgcgat
4201 cgccgcactc ggctactcgt ggcacgcgtt caccgccgag gccgacccgg cgttcggggt
4261 gatctggctg ctgtgggcag tgctgtggtt catgctgttc ctgctgctcg gcctggggca
4321 cgacgcactg gggcccgccg tcgggttcgt cgcggtggcc gaaggcgtga tcaccgccgc
4381 cgtgccggcc ttcctgatcg tgtcgggcaa ctgggaaacc ggcccgctcc ccgccgcggt
4441 catcgccgtg atcggttttg ccgcagttgt tctcgcatac cccatcgggc gccgtctcgc
4501 agcgccgtca gtcaccaacc ctccaccggc cgcgctcgcg gccaccaccc gataagagaa
4561 agggagtcca catatggctg aaaatgctgt caccaagcag gattcgccca aggcacccga
4621 gacgatctcg caagtgctgc aggtgctcgt gccgcagctg gcgcgtgcag tgcccaaggg
4681 tatggacccc gaccgcatcg cgcggatcgt gcaaaccgag atccgcaagt cgcgcaacgc
4741 gaaagctgct ggcatcgcta agcagtccct cgacgactgc acgcaagagt catttgccgg
4801 tgcgctgctg acctcggccg cgctcggtct cgagcccggt gtcaacggcg agtgctacct
4861 cgtgccctac cgcgacaccc ggcgcggcgt ggtcgagtgc cagctgatca tcggctacca
4921 gggcatcgtc aaactgttct ggcagcaccc gcgcgcctcc cggatcgacg cgcagtgggt
4981 cggcgcgaac gacgaattcc attacaccat gggcctcaat ccgacgctga aacacgtgaa
5041 ggccaagggt gatcggggta atccggtcta cttctacgcg atcgtcgagg tgaccggcgc
5101 tgagccgctg tgggacgtgt tcaccgccga cgagatcagg gaattgcgtc gcggcaaggt
5161 cggatcctcg ggcgacatca aggacccgca gcgctggatg gagcggaaga ccgcgctcaa
5221 acaggtgctg aagctggcac cgaagacgac gcggctcgac gcggcgatcc gcgccgacga
5281 tcgcccgggc accgacctgt cacagtcgca ggcgctcgcg ctgccgtcga ccgtcaagcc
5341 gacggccgac tacatcgacg gcgagatcgc cgagccgcac gaggtcgata cgccaccgaa
5401 gtcgtcgcgc gcacaacgcg cgcagcgcgc caccgcgccg gcgcccgacg tgcagatggc
5461 caatcccgat cagctgaagc gcctcggcga gatccagaag gccgaaaagt acaacgacgc
5521 cgattggttc aagttcctcg ccgattcggc cggcgtcaaa gccacgcgcg ccgccgacct
5581 cacgttcgac gaggcgaagg ctgtcatcga catgttcgac gggcccaacg catgagcgcc
5641 ccggcgaatt ccgacgcggt ggttgatctg caactagcgt acgatcgact gccaggcatc
5701 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatgcca tcatggccgc
5761 ggtgatcagc tagccacctg acgtcggggg ggggggaaag ccacgttgtg tctcaaaatc
5821 tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt
5881 acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg tcttgctcga
5941 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
6001 atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagccccat gcgccagagt
6061 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
6121 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
6181 atgatgcatg gttactcacc actgcgatcc ccgggaaaac agcattccag gtattagaag
6241 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
6301 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg
6361 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
6421 gctggcctgt tgaacaagtc tggaaagaaa tgcataatct tttgccattc tcaccggatt
6481 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
6541 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
6601 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
6661 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct
6721 aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg
6781 gcggctttgt tgaataaatc gaacttttgc tgagttgaag gatcagatca cgcatcttcc
6841 cgacaacgca gaccgttccg tggcaaagca aaagttcaaa atcaccaact ggtccaccta
6901 caacaaagct ctcaccaacc gtggctccct cactttctgg ctggatgatg gggcgattca
6961 ggcctggtat gagtcagcaa caccttcttc acgaggcaga cctcactagt tccatgagcg
7021 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
7081 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
7141 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
7201 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
7261 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
7321 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
7381 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
7441 gagctatgag aaagcgccac gcttcccgag gggagaaagg cggacaggta tccggtaagc
7501 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
7561 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
7621 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
7681 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
7741 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgc
//
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