pCDH-CMV-MCS-EF1-copGFP

此序列来自于测序结果

LOCUS       pCDH-CMV-MCS-EF1-copGFP        7541 bp ds-DNA     circular SYN 25-JUL-2016
DEFINITION  pCDH-CMV-MCS-EF1-copGFP.
SOURCE      http://www.biofeng.com
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, July 25, 2016 from SnapGene Viewer 3.1.4 to Vector 
            NTI(R) format
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|776304000|
COMMENT     VNTDBDATE|776304000|
COMMENT     VNTNAME|pCDH-CMV-MCS-EF1-copGFP|
FEATURES             Location/Qualifiers
     misc_feature    7..234
                     /label=RSV
     misc_feature    235..414
                     /label=5'LTR
                     /note="HIV-1 5'LTR"
     misc_feature    522..566
                     /label=HIV-1_psi_pack
     misc_feature    567..919
                     /label=Gag
     CDS             954..1454
                     /codon_start=1
                     /label=ORF frame 3
     misc_feature    1076..1308
                     /label=RRE
     CDS             1309..1797
                     /codon_start=1
                     /label=Env
     misc_feature    1798..1916
                     /label=cPPT
     promoter        1922..2271
                     /label=CMV promoter
     primer_bind     2164..2184
                     /label=CMV_fwd_primer
     primer_bind     2208..2227
                     /label=pCEP_fwd_primer
     primer_bind     2210..2234
                     /label=LNCX_primer
     misc_feature    2278..2320
                     /label=MCS
     promoter        2315..2860
                     /label=EF1-alpha promoter
                     /note="EF1-alpha promoter"
     misc_feature    2875..3629
                     /label=copGFP
     misc_feature    3640..4229
                     /label=WPRE
     CDS             3726..4271
                     /codon_start=1
                     /label=ORF frame 2
     CDS             3739..4329
                     /codon_start=1
                     /label=ORF frame 3
     misc_feature    4291..4312
                     /label=U3PPT
     misc_feature    4291..4306
                     /label=cPPT
     misc_feature    4301..4534
                     /label=3-Delta-LTR
                     /note="truncHIV-1 3-Delta-LTR"
     misc_feature    4308..4360
                     /label=Delta-U3
                     /note="Delta-U3"
     misc_feature    4361..4541
                     /label=HIV-1 5'LTR
     terminator      4616..4747
                     /label=SV40 PA
                     /note="SV40 PA terminator"
     misc_feature    4704..4723
                     /label=EBV_rev_primer
     rep_origin      4746..4892
                     /direction=RIGHT
                     /label=SV40 ori
                     /note="SV40 origin"
     misc_feature    4819..4834
                     /label=SV40pro_F_primer
     promoter        complement(4922..4940)
                     /label=M13_reverse_primer promoter
     misc_feature    4939..4961
                     /label=M13_pUC_rev_primer
     promoter        complement(4975..5004)
                     /label=lac promoter
     repeat_region   complement(5262..5935)
                     /label=pUC Ori
                     /note="complementary chain"
     misc_feature    complement(6080..6940)
                     /gene="Ampicillin"
                     /label=Ampicillin
     CDS             complement(6087..6947)
                     /codon_start=1
                     /label=ORF frame 2
     promoter        complement(6989..7017)
                     /label=AmpR promoter
     misc_feature    7176..7198
                     /label=pGEX_3_primer
     misc_feature    complement(7397..7540)
                     /label=lacZ a
     misc_feature    7511..7533
                     /label=M13_pUC_fwd_primer
     promoter        7526..7541
                     /label=M13_forward20_primer promoter
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgaatt taaatcggat ccgcggccgc gaaggatctg cgatcgctcc
     2341 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     2401 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     2461 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     2521 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg
     2581 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg
     2641 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta
     2701 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga
     2761 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg
     2821 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agatggagag
     2881 cgacgagagc ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg
     2941 cgtggagttc gagctggtgg gcggcggaga gggcaccccc aagcagggcc gcatgaccaa
     3001 caagatgaag agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat
     3061 gggctacggc ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca
     3121 cgccatcaac aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt
     3181 gctgcacgtg agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt
     3241 ggtgggcacc ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa
     3301 cgccaccgtg gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg
     3361 caccttcagc ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt
     3421 caagagcgcc atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg
     3481 cgtggaggag ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa
     3541 gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg tggacggcac
     3601 cgccggaccc ggctccaccg gatctcgctg tcgacaatca acctctggat tacaaaattt
     3661 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     3721 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     3781 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     3841 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     3901 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     3961 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     4021 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     4081 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     4141 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     4201 tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct
     4261 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     4321 cgaaaataag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     4381 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     4441 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     4501 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     4561 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     4621 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     4681 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     4741 ctatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     4801 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     4861 gaggcttttt tggaggccta gacttttgca gagacggccc aaattcgtaa tcatggtcat
     4921 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     4981 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     5041 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     5101 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     5161 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     5221 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     5281 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     5341 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     5401 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     5461 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     5521 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     5581 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     5641 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     5701 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     5761 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     5821 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     5881 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     5941 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     6001 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     6061 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     6121 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     6181 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     6241 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     6301 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     6361 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     6421 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     6481 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     6541 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     6601 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     6661 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     6721 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     6781 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     6841 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     6901 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     6961 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     7021 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     7081 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
     7141 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     7201 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     7261 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     7321 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt
     7381 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     7441 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
     7501 cccagtcacg acgttgtaaa acgacggcca gtgccaagct g
//