LOCUS pGIPZ 11688 bp DNA SYN
DEFINITION pGIPZ
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..11688
/organism="pGIPZ"
/mol_type="other DNA"
misc_feature 454..634
/label="HIV-1_5_LTR"
misc_feature 454..634
/label="truncHIV-1_3_LTR"
misc_feature 745..789
/label="HIV-1_psi_pack"
CDS complement(1015..1476)
/label="ORF frame 1"
CDS 1181..2068
/label="ORF frame 2"
misc_feature 1303..1536
/label="RRE"
misc_feature 2067..2082
/label="cPPT"
misc_feature complement(2245..2619)
/label="sh_ble"
gene complement(2248..2619)
/label="bleo"
/gene="bleo"
promoter complement(2629..2647)
/label="T7_promoter"
promoter 2740..3292
/label="CMV_immearly_promoter"
misc_feature 2795..3082
/label="CAG_enhancer"
misc_feature 3249..3269
/label="CMV_fwd_primer"
promoter 3250..3319
/label="CMV_promoter"
misc_feature 3293..3312
/label="pCEP_fwd_primer"
misc_feature 3295..3319
/label="LNCX_primer"
CDS 3390..4088
/label="ORF frame 3"
misc_feature 4114..4677
/label="IRES"
CDS 4690..5292
/label="ORF frame 1"
gene 4705..5292
/label="puro (variant)"
/gene="puro (variant)"
CDS 5606..6280
/label="ORF frame 2"
promoter complement(5625..5643)
/label="T7_promoter"
misc_feature 5665..6240
/label="WPRE"
misc_feature 6458..6510
/label="delta_U3"
misc_feature 6511..6691
/label="HIV-1_5_LTR"
misc_feature 6511..6691
/label="truncHIV-1_3_LTR"
misc_feature complement(6792..6809)
/label="BGH_rev_primer"
terminator 6795..7022
/label="bGH_PA_terminator"
rep_origin 7085..7391
/label="f1_origin"
misc_feature complement(7505..7525)
/label="pBABE_3_primer"
misc_feature complement(7511..7726)
/label="SV40_enhancer"
promoter 7523..7791
/label="SV40_promoter"
rep_origin 7690..7767
/label="SV40_origin"
misc_feature 7752..7771
/label="SV40pro_F_primer"
CDS complement(7825..8646)
/label="ORF frame 1"
CDS 7888..8913
/label="ORF frame 1"
gene 7903..8910
/label="hygroB"
/gene="hygroB"
terminator 9046..9165
/label="SV40_PA_terminator"
misc_feature 9134..9153
/label="EBV_rev_primer"
promoter complement(9209..9227)
/label="M13_reverse_primer"
misc_feature complement(9226..9248)
/label="M13_pUC_rev_primer"
promoter complement(9262..9291)
/label="lac_promoter"
gene complement(10374..11234)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(10374..11234)
/label="ORF frame 2"
promoter complement(11276..11304)
/label="AmpR_promoter"
terminator 11390..11521
/label="SV40_PA_terminator"
misc_feature 11478..11497
/label="EBV_rev_primer"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
2701 cgaggttcta gacgtattac cgccatgcat tagttattaa tagtaatcaa ttacggggtc
2761 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
2821 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
2881 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
2941 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
3001 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
3061 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
3121 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
3181 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
3241 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
3301 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
3361 acaccgactc tactagagga tctgccacca tggagagcga cgagagcggc ctgcccgcca
3421 tggagatcga gtgccgcatc accggcaccc tgaacggcgt ggagttcgag ctggtgggcg
3481 gcggagaggg cacccccgag cagggccgca tgaccaacaa gatgaagagc accaaaggcg
3541 ccctgacctt cagcccctac ctgctgagcc acgtgatggg ctacggcttc taccacttcg
3601 gcacctaccc cagcggctac gagaacccct tcctgcacgc catcaacaac ggcggctaca
3661 ccaacacccg catcgagaag tacgaggacg gcggcgtgct gcacgtgagc ttcagctacc
3721 gctacgaggc cggccgcgtg atcggcgact tcaaggtgat gggcaccggc ttccccgagg
3781 acagcgtgat cttcaccgac aagatcatcc gcagcaacgc caccgtggag cacctgcacc
3841 ccatgggcga taacgatctg gatggcagct tcacccgcac cttcagcctg cgcgacggcg
3901 gctactacag ctccgtggtg gacagccaca tgcacttcaa gagcgccatc caccccagca
3961 tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt ggaggaggat cacagcaaca
4021 ccgagctggg catcgtggag taccagcacg ccttcaagac cccggatgca gatgccggtg
4081 aagaataatg tacaagtagc ggccgcaaat tccgcccctc tccctccccc ccccctaacg
4141 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca
4201 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga
4261 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga
4321 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca
4381 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag
4441 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa
4501 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac
4561 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga
4621 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
4681 gataatacca tggccaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
4741 ccccgggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
4801 gtcgacccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
4861 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
4921 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggctc gcgcatggcc
4981 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
5041 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
5101 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgctggggtg
5161 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
5221 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
5281 cccggtgcct gagtttgttt gaatgaggct tcagtacttt acagaatcgt tgcctgcaca
5341 tcttggaaac acttgctggg attacttctt caggttaacc caacagaagg ctcgaggtaa
5401 ccggatcctg atcagaattc aaggggctac tttaggagca attatcttgt ttactaaaac
5461 tgaatacctt gctatctctt tgatacattt ttacaaagct gaattaaaat ggtataaatt
5521 aaatcacttt tttcaattgg aagactaatg cggccggcca ttactccgtc tcgtgtcttg
5581 ttgcatatgt ctgctggttt gtttgatgtt gtttgcgggc gggccctata gtgagtcgta
5641 ttacctagga cgcgtctgga acaatcaacc tctggattac aaaatttgtg aaagattgac
5701 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
5761 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
5821 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
5881 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
5941 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
6001 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
6061 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
6121 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
6181 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
6241 cgcctccccg cctggaatta attctgcagt cgagacctag aaaaacatgg agcaatcaca
6301 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag
6361 gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct
6421 gtagatctta gccacttttt aaaagaaaag aggggactgg aagggctaat tcactcccaa
6481 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
6541 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
6601 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
6661 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
6721 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttgtttaaac
6781 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
6841 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
6901 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
6961 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
7021 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
7081 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
7141 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
7201 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
7261 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
7321 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
7381 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
7441 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
7501 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
7561 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
7621 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
7681 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
7741 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
7801 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
7861 tccattttcg gatctgatca gcacgtgatg aaaaagcctg aactcaccgc gacgtctgtc
7921 gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc
7981 gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat
8041 agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg
8101 ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc
8161 tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt
8221 ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc
8281 gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata
8341 tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt
8401 gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc
8461 cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata
8521 acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac
8581 atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg
8641 aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt
8701 gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt
8761 cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc
8821 agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga
8881 cgccccagca ctcgtccgag ggcaaaggaa tagcacgtgc tacgagattt cgattccacc
8941 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
9001 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
9061 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
9121 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
9181 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
9241 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
9301 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
9361 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
9421 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
9481 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
9541 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
9601 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
9661 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
9721 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
9781 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
9841 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
9901 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
9961 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
10021 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
10081 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
10141 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
10201 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
10261 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
10321 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
10381 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
10441 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
10501 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
10561 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
10621 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
10681 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
10741 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
10801 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
10861 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
10921 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
10981 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
11041 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
11101 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
11161 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
11221 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
11281 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
11341 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcaact tgtttattgc
11401 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
11461 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
11521 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac
11581 cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc
11641 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagt
//