hCas9_D10A

价格:1200元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

买家导航

载体基本信息

质粒名称: hCas9_D10A 
出品公司: 爱迪基因
目录编号: 41816
存储实验室: George Church

载体骨架基本信息
载体名称: pcDNA3.3-TOPO
载体出品公司: Invitrogen
载体抗性: 氨苄霉素
载体类型: 哺乳细胞表达,CRISPR
高拷贝/低拷贝: 未知
生长条件: 37 ℃

插入基因信息
插入基因名称: Cas9_D10A
插入大小: 4140 bp
基因突变: human codon-optimized, D10A nickase
启动子 CMV
克隆方法: TOPO Cloning
5' 测序引物 CMV-F

文献引用方法
材料和方法部分: hCas9_D10A was a gift from George Church (Addgene plasmid # 41816)
参考文献部分: RNA-Guided Human Genome Engineering via Cas9. Mali P, Yang L, Esvelt KM, Aach J, Guell M, Dicarlo JE, Norville JE, Church GM. Science. 2013 Jan 3. 10.1126/science.1232033 PubMed 23287722

载体图谱质粒图谱和多克隆位点信息

hCas9_D10A载体图谱

载体简介

载体序列

LOCUS       hCas9_D10A Sequencing Result	9553 bp 	DNA	SYN
DEFINITION  hCas9_D10A Sequencing Result
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..9553
                     /organism="hCas9_D10A Sequencing Result"
                     /mol_type="other DNA"
     misc_feature    155..175
                     /label="CMV_fwd_primer"
     promoter        156..225
                     /label="CMV_promoter"
     CDS             160..4458
                     /label="ORF frame 1"
     promoter        168..287
                     /label="CMV2_promoter"
     misc_feature    199..218
                     /label="pCEP_fwd_primer"
     misc_feature    201..225
                     /label="LNCX_primer"
     CDS             complement(295..798)
                     /label="ORF frame 2"
     CDS             complement(1723..2241)
                     /label="ORF frame 2"
     CDS             complement(2305..2838)
                     /label="ORF frame 2"
     CDS             complement(3832..4365)
                     /label="ORF frame 2"
     misc_feature    4435..4455
                     /label="SV40 NLS"
     misc_feature    4441..4455
                     /label="NLS"
     terminator      4497..4767
                     /label="TK_PA_terminator"
     rep_origin      4821..5127
                     /label="f1_origin"
     misc_feature    complement(5241..5261)
                     /label="pBABE_3_primer"
     misc_feature    complement(5247..5462)
                     /label="SV40_enhancer"
     promoter        5259..5527
                     /label="SV40_promoter"
     rep_origin      5426..5503
                     /label="SV40_origin"
     misc_feature    5488..5507
                     /label="SV40pro_F_primer"
     CDS             5642..6436
                     /label="ORF frame 2"
     gene            5645..6433
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
     terminator      6615..6734
                     /label="SV40_PA_terminator"
     misc_feature    6703..6722
                     /label="EBV_rev_primer"
     promoter        complement(6778..6796)
                     /label="M13_reverse_primer"
     misc_feature    complement(6795..6817)
                     /label="M13_pUC_rev_primer"
     promoter        complement(6831..6860)
                     /label="lac_promoter"
     gene            complement(7943..8803)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(7943..8803)
                     /label="ORF frame 1"
     promoter        complement(8845..8873)
                     /label="AmpR_promoter"
     promoter        9175..198
                     /label="CMV_immearly_promoter"
     misc_feature    9254..9541
                     /label="CAG_enhancer"
ORIGIN
    1 GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT
   61 CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC
  121 TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG
  181 TGGGAGGTCT ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGATCGCCTG GAGACGCCAT
  241 CCACGCTGTT TTGACCTCCA TAGAAGACAC CGGGACCGAT CCAGCCTCCG GACTCTAGAG
  301 GATCGAACCC TTGCCACCAT GGACAAGAAG TACTCCATTG GGCTCGCTAT CGGCACAAAC
  361 AGCGTCGGCT GGGCCGTCAT TACGGACGAG TACAAGGTGC CGAGCAAAAA ATTCAAAGTT
  421 CTGGGCAATA CCGATCGCCA CAGCATAAAG AAGAACCTCA TTGGCGCCCT CCTGTTCGAC
  481 TCCGGGGAGA CGGCCGAAGC CACGCGGCTC AAAAGAACAG CACGGCGCAG ATATACCCGC
  541 AGAAAGAATC GGATCTGCTA CCTGCAGGAG ATCTTTAGTA ATGAGATGGC TAAGGTGGAT
  601 GACTCTTTCT TCCATAGGCT GGAGGAGTCC TTTTTGGTGG AGGAGGATAA AAAGCACGAG
  661 CGCCACCCAA TCTTTGGCAA TATCGTGGAC GAGGTGGCGT ACCATGAAAA GTACCCAACC
  721 ATATATCATC TGAGGAAGAA GCTTGTAGAC AGTACTGATA AGGCTGACTT GCGGTTGATC
  781 TATCTCGCGC TGGCGCATAT GATCAAATTT CGGGGACACT TCCTCATCGA GGGGGACCTG
  841 AACCCAGACA ACAGCGATGT CGACAAACTC TTTATCCAAC TGGTTCAGAC TTACAATCAG
  901 CTTTTCGAAG AGAACCCGAT CAACGCATCC GGAGTTGACG CCAAAGCAAT CCTGAGCGCT
  961 AGGCTGTCCA AATCCCGGCG GCTCGAAAAC CTCATCGCAC AGCTCCCTGG GGAGAAGAAG
 1021 AACGGCCTGT TTGGTAATCT TATCGCCCTG TCACTCGGGC TGACCCCCAA CTTTAAATCT
 1081 AACTTCGACC TGGCCGAAGA TGCCAAGCTT CAACTGAGCA AAGACACCTA CGATGATGAT
 1141 CTCGACAATC TGCTGGCCCA GATCGGCGAC CAGTACGCAG ACCTTTTTTT GGCGGCAAAG
 1201 AACCTGTCAG ACGCCATTCT GCTGAGTGAT ATTCTGCGAG TGAACACGGA GATCACCAAA
 1261 GCTCCGCTGA GCGCTAGTAT GATCAAGCGC TATGATGAGC ACCACCAAGA CTTGACTTTG
 1321 CTGAAGGCCC TTGTCAGACA GCAACTGCCT GAGAAGTACA AGGAAATTTT CTTCGATCAG
 1381 TCTAAAAATG GCTACGCCGG ATACATTGAC GGCGGAGCAA GCCAGGAGGA ATTTTACAAA
 1441 TTTATTAAGC CCATCTTGGA AAAAATGGAC GGCACCGAGG AGCTGCTGGT AAAGCTTAAC
 1501 AGAGAAGATC TGTTGCGCAA ACAGCGCACT TTCGACAATG GAAGCATCCC CCACCAGATT
 1561 CACCTGGGCG AACTGCACGC TATCCTCAGG CGGCAAGAGG ATTTCTACCC CTTTTTGAAA
 1621 GATAACAGGG AAAAGATTGA GAAAATCCTC ACATTTCGGA TACCCTACTA TGTAGGCCCC
 1681 CTCGCCCGGG GAAATTCCAG ATTCGCGTGG ATGACTCGCA AATCAGAAGA GACCATCACT
 1741 CCCTGGAACT TCGAGGAAGT CGTGGATAAG GGGGCCTCTG CCCAGTCCTT CATCGAAAGG
 1801 ATGACTAACT TTGATAAAAA TCTGCCTAAC GAAAAGGTGC TTCCTAAACA CTCTCTGCTG
 1861 TACGAGTACT TCACAGTTTA TAACGAGCTC ACCAAGGTCA AATACGTCAC AGAAGGGATG
 1921 AGAAAGCCAG CATTCCTGTC TGGAGAGCAG AAGAAAGCTA TCGTGGACCT CCTCTTCAAG
 1981 ACGAACCGGA AAGTTACCGT GAAACAGCTC AAAGAAGACT ATTTCAAAAA GATTGAATGT
 2041 TTCGACTCTG TTGAAATCAG CGGAGTGGAG GATCGCTTCA ACGCATCCCT GGGAACGTAT
 2101 CACGATCTCC TGAAAATCAT TAAAGACAAG GACTTCCTGG ACAATGAGGA GAACGAGGAC
 2161 ATTCTTGAGG ACATTGTCCT CACCCTTACG TTGTTTGAAG ATAGGGAGAT GATTGAAGAA
 2221 CGCTTGAAAA CTTACGCTCA TCTCTTCGAC GACAAAGTCA TGAAACAGCT CAAGAGGCGC
 2281 CGATATACAG GATGGGGGCG GCTGTCAAGA AAACTGATCA ATGGGATCCG AGACAAGCAG
 2341 AGTGGAAAGA CAATCCTGGA TTTTCTTAAG TCCGATGGAT TTGCCAACAG GAACTTCATG
 2401 CAGTTGATCC ATGATGACTC TCTCACCTTT AAGGAGGACA TCCAGAAAGC ACAAGTTTCT
 2461 GGCCAGGGGG ACAGTCTTCA CGAGCACATC GCTAATCTTG CAGGTAGCCC AGCTATCAAA
 2521 AAGGGAATAC TGCAGACCGT TAAGGTCGTG GATGAACTCG TCAAAGTAAT GGGAAGGCAT
 2581 AAGCCCGAGA ATATCGTTAT CGAGATGGCC CGAGAGAACC AAACTACCCA GAAGGGACAG
 2641 AAGAACAGTA GGGAAAGGAT GAAGAGGATT GAAGAGGGTA TAAAAGAACT GGGGTCCCAA
 2701 ATCCTTAAGG AACACCCAGT TGAAAACACC CAGCTTCAGA ATGAGAAGCT CTACCTGTAC
 2761 TACCTGCAGA ACGGCAGGGA CATGTACGTG GATCAGGAAC TGGACATCAA TCGGCTCTCC
 2821 GACTACGACG TGGATCATAT CGTGCCCCAG TCTTTTCTCA AAGATGATTC TATTGATAAT
 2881 AAAGTGTTGA CAAGATCCGA TAAAAATAGA GGGAAGAGTG ATAACGTCCC CTCAGAAGAA
 2941 GTTGTCAAGA AAATGAAAAA TTATTGGCGG CAGCTGCTGA ACGCCAAACT GATCACACAA
 3001 CGGAAGTTCG ATAATCTGAC TAAGGCTGAA CGAGGTGGCC TGTCTGAGTT GGATAAAGCC
 3061 GGCTTCATCA AAAGGCAGCT TGTTGAGACA CGCCAGATCA CCAAGCACGT GGCCCAAATT
 3121 CTCGATTCAC GCATGAACAC CAAGTACGAT GAAAATGACA AACTGATTCG AGAGGTGAAA
 3181 GTTATTACTC TGAAGTCTAA GCTGGTCTCA GATTTCAGAA AGGACTTTCA GTTTTATAAG
 3241 GTGAGAGAGA TCAACAATTA CCACCATGCG CATGATGCCT ACCTGAATGC AGTGGTAGGC
 3301 ACTGCACTTA TCAAAAAATA TCCCAAGCTT GAATCTGAAT TTGTTTACGG AGACTATAAA
 3361 GTGTACGATG TTAGGAAAAT GATCGCAAAG TCTGAGCAGG AAATAGGCAA GGCCACCGCT
 3421 AAGTACTTCT TTTACAGCAA TATTATGAAT TTTTTCAAGA CCGAGATTAC ACTGGCCAAT
 3481 GGAGAGATTC GGAAGCGACC ACTTATCGAA ACAAACGGAG AAACAGGAGA AATCGTGTGG
 3541 GACAAGGGTA GGGATTTCGC GACAGTCCGG AAGGTCCTGT CCATGCCGCA GGTGAACATC
 3601 GTTAAAAAGA CCGAAGTACA GACCGGAGGC TTCTCCAAGG AAAGTATCCT CCCGAAAAGG
 3661 AACAGCGACA AGCTGATCGC ACGCAAAAAA GATTGGGACC CCAAGAAATA CGGCGGATTC
 3721 GATTCTCCTA CAGTCGCTTA CAGTGTACTG GTTGTGGCCA AAGTGGAGAA AGGGAAGTCT
 3781 AAAAAACTCA AAAGCGTCAA GGAACTGCTG GGCATCACAA TCATGGAGCG ATCAAGCTTC
 3841 GAAAAAAACC CCATCGACTT TCTCGAGGCG AAAGGATATA AAGAGGTCAA AAAAGACCTC
 3901 ATCATTAAGC TTCCCAAGTA CTCTCTCTTT GAGCTTGAAA ACGGCCGGAA ACGAATGCTC
 3961 GCTAGTGCGG GCGAGCTGCA GAAAGGTAAC GAGCTGGCAC TGCCCTCTAA ATACGTTAAT
 4021 TTCTTGTATC TGGCCAGCCA CTATGAAAAG CTCAAAGGGT CTCCCGAAGA TAATGAGCAG
 4081 AAGCAGCTGT TCGTGGAACA ACACAAACAC TACCTTGATG AGATCATCGA GCAAATAAGC
 4141 GAATTCTCCA AAAGAGTGAT CCTCGCCGAC GCTAACCTCG ATAAGGTGCT TTCTGCTTAC
 4201 AATAAGCACA GGGATAAGCC CATCAGGGAG CAGGCAGAAA ACATTATCCA CTTGTTTACT
 4261 CTGACCAACT TGGGCGCGCC TGCAGCCTTC AAGTACTTCG ACACCACCAT AGACAGAAAG
 4321 CGGTACACCT CTACAAAGGA GGTCCTGGAC GCCACACTGA TTCATCAGTC AATTACGGGG
 4381 CTCTATGAAA CAAGAATCGA CCTCTCTCAG CTCGGTGGAG ACAGCAGGGC TGACCCCAAG
 4441 AAGAAGAGGA AGGTGTGAAA GGGTTCGATC CCTACCGGTT AGTAATGAGT TTAAACGGGG
 4501 GAGGCTAACT GAAACACGGA AGGAGACAAT ACCGGAAGGA ACCCGCGCTA TGACGGCAAT
 4561 AAAAAGACAG AATAAAACGC ACGGGTGTTG GGTCGTTTGT TCATAAACGC GGGGTTCGGT
 4621 CCCAGGGCTG GCACTCTGTC GATACCCCAC CGAGACCCCA TTGGGGCCAA TACGCCCGCG
 4681 TTTCTTCCTT TTCCCCACCC CACCCCCCAA GTTCGGGTGA AGGCCCAGGG CTCGCAGCCA
 4741 ACGTCGGGGC GGCAGGCCCT GCCATAGCAG ATCTGCGCAG CTGGGGCTCT AGGGGGTATC
 4801 CCCACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA
 4861 CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG
 4921 CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT
 4981 TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG
 5041 GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA
 5101 GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT
 5161 TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT
 5221 TTAACGCGAA TTAATTCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC
 5281 CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA
 5341 GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC
 5401 CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC
 5461 TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCTGCCTC
 5521 TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT
 5581 CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG
 5641 CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT
 5701 CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC
 5761 AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT
 5821 GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT
 5881 GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA
 5941 GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT
 6001 GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG
 6061 CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA
 6121 AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA
 6181 CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA
 6241 TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA
 6301 CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT
 6361 CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT
 6421 TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGCGAAAT GACCGACCAA GCGACGCCCA
 6481 ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA
 6541 TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT
 6601 TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA
 6661 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA
 6721 TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT
 6781 GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG
 6841 CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG
 6901 CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG cattaatgaa
 6961 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctCTTCCGCT TCCTCGCTCA
 7021 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG
 7081 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC
 7141 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC
 7201 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC
 7261 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC
 7321 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA
 7381 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC
 7441 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA
 7501 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG
 7561 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA
 7621 GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG
 7681 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC
 7741 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT
 7801 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA
 7861 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT
 7921 ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA
 7981 TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC
 8041 GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG
 8101 CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG
 8161 CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT
 8221 CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT
 8281 CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT
 8341 CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA
 8401 AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA
 8461 TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT
 8521 AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC
 8581 ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA
 8641 GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT
 8701 CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG
 8761 CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT
 8821 ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT
 8881 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCG
 8941 ACGGATCGGG AGATCTCCCG ATCCCCTATG GTGCACTCTC AGTACAATCT GCTCTGATGC
 9001 CGCATAGTTA AGCCAGTATC TGCTCCCTGC TTGTGTGTTG GAGGTCGCTG AGTAGTGCGC
 9061 GAGCAAAATT TAAGCTACAA CAAGGCAAGG CTTGACCGAC AATTGCATGA AGAATCTGCT
 9121 TAGGGTTAGG CGTTTTGCGC TGCTTCGCGA TGTACGGGCC AGATATACGC GTTGACATTG
 9181 ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA GCCCATATAT
 9241 GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC CCAACGACCC
 9301 CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG GGACTTTCCA
 9361 TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC TTGGCAGTAC ATCAAGTGTA
 9421 TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG CCTGGCATTA
 9481 TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG TATTAGTCAT
 9541 CGCTATTACC ATG
//

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