质粒名称: | hCas9_D10A |
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出品公司: | 爱迪基因 |
目录编号: | 41816 |
存储实验室: | George Church |
载体骨架基本信息 |
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载体名称: | pcDNA3.3-TOPO |
载体出品公司: | Invitrogen |
载体抗性: | 氨苄霉素 |
载体类型: | 哺乳细胞表达,CRISPR |
高拷贝/低拷贝: | 未知 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | Cas9_D10A |
插入大小: | 4140 bp |
基因突变: | human codon-optimized, D10A nickase |
启动子 | CMV |
克隆方法: | TOPO Cloning |
5' 测序引物 | CMV-F |
文献引用方法 |
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材料和方法部分: | hCas9_D10A was a gift from George Church (Addgene plasmid # 41816) |
参考文献部分: | RNA-Guided Human Genome Engineering via Cas9. Mali P, Yang L, Esvelt KM, Aach J, Guell M, Dicarlo JE, Norville JE, Church GM. Science. 2013 Jan 3. 10.1126/science.1232033 PubMed 23287722 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS hCas9_D10A Sequencing Result 9553 bp DNA SYN DEFINITION hCas9_D10A Sequencing Result ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..9553 /organism="hCas9_D10A Sequencing Result" /mol_type="other DNA" misc_feature 155..175 /label="CMV_fwd_primer" promoter 156..225 /label="CMV_promoter" CDS 160..4458 /label="ORF frame 1" promoter 168..287 /label="CMV2_promoter" misc_feature 199..218 /label="pCEP_fwd_primer" misc_feature 201..225 /label="LNCX_primer" CDS complement(295..798) /label="ORF frame 2" CDS complement(1723..2241) /label="ORF frame 2" CDS complement(2305..2838) /label="ORF frame 2" CDS complement(3832..4365) /label="ORF frame 2" misc_feature 4435..4455 /label="SV40 NLS" misc_feature 4441..4455 /label="NLS" terminator 4497..4767 /label="TK_PA_terminator" rep_origin 4821..5127 /label="f1_origin" misc_feature complement(5241..5261) /label="pBABE_3_primer" misc_feature complement(5247..5462) /label="SV40_enhancer" promoter 5259..5527 /label="SV40_promoter" rep_origin 5426..5503 /label="SV40_origin" misc_feature 5488..5507 /label="SV40pro_F_primer" CDS 5642..6436 /label="ORF frame 2" gene 5645..6433 /label="NeoR/KanR" /gene="NeoR/KanR" terminator 6615..6734 /label="SV40_PA_terminator" misc_feature 6703..6722 /label="EBV_rev_primer" promoter complement(6778..6796) /label="M13_reverse_primer" misc_feature complement(6795..6817) /label="M13_pUC_rev_primer" promoter complement(6831..6860) /label="lac_promoter" gene complement(7943..8803) /label="Ampicillin" /gene="Ampicillin" CDS complement(7943..8803) /label="ORF frame 1" promoter complement(8845..8873) /label="AmpR_promoter" promoter 9175..198 /label="CMV_immearly_promoter" misc_feature 9254..9541 /label="CAG_enhancer" ORIGIN 1 GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT 61 CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC 121 TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG 181 TGGGAGGTCT ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGATCGCCTG GAGACGCCAT 241 CCACGCTGTT TTGACCTCCA TAGAAGACAC CGGGACCGAT CCAGCCTCCG GACTCTAGAG 301 GATCGAACCC TTGCCACCAT GGACAAGAAG TACTCCATTG GGCTCGCTAT CGGCACAAAC 361 AGCGTCGGCT GGGCCGTCAT TACGGACGAG TACAAGGTGC CGAGCAAAAA ATTCAAAGTT 421 CTGGGCAATA CCGATCGCCA CAGCATAAAG AAGAACCTCA TTGGCGCCCT CCTGTTCGAC 481 TCCGGGGAGA CGGCCGAAGC CACGCGGCTC AAAAGAACAG CACGGCGCAG ATATACCCGC 541 AGAAAGAATC GGATCTGCTA CCTGCAGGAG ATCTTTAGTA ATGAGATGGC TAAGGTGGAT 601 GACTCTTTCT TCCATAGGCT GGAGGAGTCC TTTTTGGTGG AGGAGGATAA AAAGCACGAG 661 CGCCACCCAA TCTTTGGCAA TATCGTGGAC GAGGTGGCGT ACCATGAAAA GTACCCAACC 721 ATATATCATC TGAGGAAGAA GCTTGTAGAC AGTACTGATA AGGCTGACTT GCGGTTGATC 781 TATCTCGCGC TGGCGCATAT GATCAAATTT CGGGGACACT TCCTCATCGA GGGGGACCTG 841 AACCCAGACA ACAGCGATGT CGACAAACTC TTTATCCAAC TGGTTCAGAC TTACAATCAG 901 CTTTTCGAAG AGAACCCGAT CAACGCATCC GGAGTTGACG CCAAAGCAAT CCTGAGCGCT 961 AGGCTGTCCA AATCCCGGCG GCTCGAAAAC CTCATCGCAC AGCTCCCTGG GGAGAAGAAG 1021 AACGGCCTGT TTGGTAATCT TATCGCCCTG TCACTCGGGC TGACCCCCAA CTTTAAATCT 1081 AACTTCGACC TGGCCGAAGA TGCCAAGCTT CAACTGAGCA AAGACACCTA CGATGATGAT 1141 CTCGACAATC TGCTGGCCCA GATCGGCGAC CAGTACGCAG ACCTTTTTTT GGCGGCAAAG 1201 AACCTGTCAG ACGCCATTCT GCTGAGTGAT ATTCTGCGAG TGAACACGGA GATCACCAAA 1261 GCTCCGCTGA GCGCTAGTAT GATCAAGCGC TATGATGAGC ACCACCAAGA CTTGACTTTG 1321 CTGAAGGCCC TTGTCAGACA GCAACTGCCT GAGAAGTACA AGGAAATTTT CTTCGATCAG 1381 TCTAAAAATG GCTACGCCGG ATACATTGAC GGCGGAGCAA GCCAGGAGGA ATTTTACAAA 1441 TTTATTAAGC CCATCTTGGA AAAAATGGAC GGCACCGAGG AGCTGCTGGT AAAGCTTAAC 1501 AGAGAAGATC TGTTGCGCAA ACAGCGCACT TTCGACAATG GAAGCATCCC CCACCAGATT 1561 CACCTGGGCG AACTGCACGC TATCCTCAGG CGGCAAGAGG ATTTCTACCC CTTTTTGAAA 1621 GATAACAGGG AAAAGATTGA GAAAATCCTC ACATTTCGGA TACCCTACTA TGTAGGCCCC 1681 CTCGCCCGGG GAAATTCCAG ATTCGCGTGG ATGACTCGCA AATCAGAAGA GACCATCACT 1741 CCCTGGAACT TCGAGGAAGT CGTGGATAAG GGGGCCTCTG CCCAGTCCTT CATCGAAAGG 1801 ATGACTAACT TTGATAAAAA TCTGCCTAAC GAAAAGGTGC TTCCTAAACA CTCTCTGCTG 1861 TACGAGTACT TCACAGTTTA TAACGAGCTC ACCAAGGTCA AATACGTCAC AGAAGGGATG 1921 AGAAAGCCAG CATTCCTGTC TGGAGAGCAG AAGAAAGCTA TCGTGGACCT CCTCTTCAAG 1981 ACGAACCGGA AAGTTACCGT GAAACAGCTC AAAGAAGACT ATTTCAAAAA GATTGAATGT 2041 TTCGACTCTG TTGAAATCAG CGGAGTGGAG GATCGCTTCA ACGCATCCCT GGGAACGTAT 2101 CACGATCTCC TGAAAATCAT TAAAGACAAG GACTTCCTGG ACAATGAGGA GAACGAGGAC 2161 ATTCTTGAGG ACATTGTCCT CACCCTTACG TTGTTTGAAG ATAGGGAGAT GATTGAAGAA 2221 CGCTTGAAAA CTTACGCTCA TCTCTTCGAC GACAAAGTCA TGAAACAGCT CAAGAGGCGC 2281 CGATATACAG GATGGGGGCG GCTGTCAAGA AAACTGATCA ATGGGATCCG AGACAAGCAG 2341 AGTGGAAAGA CAATCCTGGA TTTTCTTAAG TCCGATGGAT TTGCCAACAG GAACTTCATG 2401 CAGTTGATCC ATGATGACTC TCTCACCTTT AAGGAGGACA TCCAGAAAGC ACAAGTTTCT 2461 GGCCAGGGGG ACAGTCTTCA CGAGCACATC GCTAATCTTG CAGGTAGCCC AGCTATCAAA 2521 AAGGGAATAC TGCAGACCGT TAAGGTCGTG GATGAACTCG TCAAAGTAAT GGGAAGGCAT 2581 AAGCCCGAGA ATATCGTTAT CGAGATGGCC CGAGAGAACC AAACTACCCA GAAGGGACAG 2641 AAGAACAGTA GGGAAAGGAT GAAGAGGATT GAAGAGGGTA TAAAAGAACT GGGGTCCCAA 2701 ATCCTTAAGG AACACCCAGT TGAAAACACC CAGCTTCAGA ATGAGAAGCT CTACCTGTAC 2761 TACCTGCAGA ACGGCAGGGA CATGTACGTG GATCAGGAAC TGGACATCAA TCGGCTCTCC 2821 GACTACGACG TGGATCATAT CGTGCCCCAG TCTTTTCTCA AAGATGATTC TATTGATAAT 2881 AAAGTGTTGA CAAGATCCGA TAAAAATAGA GGGAAGAGTG ATAACGTCCC CTCAGAAGAA 2941 GTTGTCAAGA AAATGAAAAA TTATTGGCGG CAGCTGCTGA ACGCCAAACT GATCACACAA 3001 CGGAAGTTCG ATAATCTGAC TAAGGCTGAA CGAGGTGGCC TGTCTGAGTT GGATAAAGCC 3061 GGCTTCATCA AAAGGCAGCT TGTTGAGACA CGCCAGATCA CCAAGCACGT GGCCCAAATT 3121 CTCGATTCAC GCATGAACAC CAAGTACGAT GAAAATGACA AACTGATTCG AGAGGTGAAA 3181 GTTATTACTC TGAAGTCTAA GCTGGTCTCA GATTTCAGAA AGGACTTTCA GTTTTATAAG 3241 GTGAGAGAGA TCAACAATTA CCACCATGCG CATGATGCCT ACCTGAATGC AGTGGTAGGC 3301 ACTGCACTTA TCAAAAAATA TCCCAAGCTT GAATCTGAAT TTGTTTACGG AGACTATAAA 3361 GTGTACGATG TTAGGAAAAT GATCGCAAAG TCTGAGCAGG AAATAGGCAA GGCCACCGCT 3421 AAGTACTTCT TTTACAGCAA TATTATGAAT TTTTTCAAGA CCGAGATTAC ACTGGCCAAT 3481 GGAGAGATTC GGAAGCGACC ACTTATCGAA ACAAACGGAG AAACAGGAGA AATCGTGTGG 3541 GACAAGGGTA GGGATTTCGC GACAGTCCGG AAGGTCCTGT CCATGCCGCA GGTGAACATC 3601 GTTAAAAAGA CCGAAGTACA GACCGGAGGC TTCTCCAAGG AAAGTATCCT CCCGAAAAGG 3661 AACAGCGACA AGCTGATCGC ACGCAAAAAA GATTGGGACC CCAAGAAATA CGGCGGATTC 3721 GATTCTCCTA CAGTCGCTTA CAGTGTACTG GTTGTGGCCA AAGTGGAGAA AGGGAAGTCT 3781 AAAAAACTCA AAAGCGTCAA GGAACTGCTG GGCATCACAA TCATGGAGCG ATCAAGCTTC 3841 GAAAAAAACC CCATCGACTT TCTCGAGGCG AAAGGATATA AAGAGGTCAA AAAAGACCTC 3901 ATCATTAAGC TTCCCAAGTA CTCTCTCTTT GAGCTTGAAA ACGGCCGGAA ACGAATGCTC 3961 GCTAGTGCGG GCGAGCTGCA GAAAGGTAAC GAGCTGGCAC TGCCCTCTAA ATACGTTAAT 4021 TTCTTGTATC TGGCCAGCCA CTATGAAAAG CTCAAAGGGT CTCCCGAAGA TAATGAGCAG 4081 AAGCAGCTGT TCGTGGAACA ACACAAACAC TACCTTGATG AGATCATCGA GCAAATAAGC 4141 GAATTCTCCA AAAGAGTGAT CCTCGCCGAC GCTAACCTCG ATAAGGTGCT TTCTGCTTAC 4201 AATAAGCACA GGGATAAGCC CATCAGGGAG CAGGCAGAAA ACATTATCCA CTTGTTTACT 4261 CTGACCAACT TGGGCGCGCC TGCAGCCTTC AAGTACTTCG ACACCACCAT AGACAGAAAG 4321 CGGTACACCT CTACAAAGGA GGTCCTGGAC GCCACACTGA TTCATCAGTC AATTACGGGG 4381 CTCTATGAAA CAAGAATCGA CCTCTCTCAG CTCGGTGGAG ACAGCAGGGC TGACCCCAAG 4441 AAGAAGAGGA AGGTGTGAAA GGGTTCGATC CCTACCGGTT AGTAATGAGT TTAAACGGGG 4501 GAGGCTAACT GAAACACGGA AGGAGACAAT ACCGGAAGGA ACCCGCGCTA TGACGGCAAT 4561 AAAAAGACAG AATAAAACGC ACGGGTGTTG GGTCGTTTGT TCATAAACGC GGGGTTCGGT 4621 CCCAGGGCTG GCACTCTGTC GATACCCCAC CGAGACCCCA TTGGGGCCAA TACGCCCGCG 4681 TTTCTTCCTT TTCCCCACCC CACCCCCCAA GTTCGGGTGA AGGCCCAGGG CTCGCAGCCA 4741 ACGTCGGGGC GGCAGGCCCT GCCATAGCAG ATCTGCGCAG CTGGGGCTCT AGGGGGTATC 4801 CCCACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA 4861 CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG 4921 CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT 4981 TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG 5041 GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA 5101 GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT 5161 TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT 5221 TTAACGCGAA TTAATTCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC 5281 CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA 5341 GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC 5401 CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC 5461 TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCTGCCTC 5521 TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT 5581 CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG 5641 CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT 5701 CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC 5761 AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT 5821 GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT 5881 GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA 5941 GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT 6001 GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG 6061 CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA 6121 AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA 6181 CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA 6241 TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA 6301 CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT 6361 CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT 6421 TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGCGAAAT GACCGACCAA GCGACGCCCA 6481 ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA 6541 TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT 6601 TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA 6661 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA 6721 TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT 6781 GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG 6841 CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG 6901 CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG cattaatgaa 6961 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctCTTCCGCT TCCTCGCTCA 7021 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG 7081 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC 7141 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC 7201 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC 7261 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC 7321 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA 7381 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC 7441 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA 7501 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG 7561 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA 7621 GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG 7681 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC 7741 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT 7801 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA 7861 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT 7921 ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA 7981 TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC 8041 GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG 8101 CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG 8161 CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT 8221 CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT 8281 CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT 8341 CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA 8401 AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA 8461 TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT 8521 AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC 8581 ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA 8641 GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT 8701 CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG 8761 CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT 8821 ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT 8881 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCG 8941 ACGGATCGGG AGATCTCCCG ATCCCCTATG GTGCACTCTC AGTACAATCT GCTCTGATGC 9001 CGCATAGTTA AGCCAGTATC TGCTCCCTGC TTGTGTGTTG GAGGTCGCTG AGTAGTGCGC 9061 GAGCAAAATT TAAGCTACAA CAAGGCAAGG CTTGACCGAC AATTGCATGA AGAATCTGCT 9121 TAGGGTTAGG CGTTTTGCGC TGCTTCGCGA TGTACGGGCC AGATATACGC GTTGACATTG 9181 ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA GCCCATATAT 9241 GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC CCAACGACCC 9301 CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG GGACTTTCCA 9361 TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC TTGGCAGTAC ATCAAGTGTA 9421 TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG CCTGGCATTA 9481 TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG TATTAGTCAT 9541 CGCTATTACC ATG //
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