质粒名称: | aMHC-eGFP-Rex-Neo |
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出品公司: | 爱迪基因 |
目录编号: | 21229 |
存储实验室: | Mark Mercola |
载体骨架基本信息 |
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载体名称: | SIN18 |
载体抗性: | 氨苄霉素 |
空载体大小: | 8400 bp |
载体类型: | 慢病毒载体 |
筛选标记: | 新霉素抗性标记Rex-1基因启动子驱动 |
高拷贝/低拷贝: | 未知 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | alpha myosin heavy chain promoter |
物种来源: | M.肌肉(老鼠) |
插入大小: | 5470 bp |
标签/融合蛋白: | eGFP(C端) |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | claI(未破坏) |
3' 克隆位点: | AgeI (未破坏) |
5' 测序引物 | CTTGATGCCGTTCTTCTGCTT - 3' Sequencing |
文献引用方法 |
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材料和方法部分: | aMHC-eGFP-Rex-Neo was a gift from Mark Mercola (Addgene plasmid # 21229) |
参考文献部分: | Lentiviral vectors and protocols for creation of stable hESC lines for fluorescent tracking and drug resistance selection of cardiomyocytes. Kita-Matsuo H, Barcova M, Prigozhina N, Salomonis N, Wei K, Jacot JG, Nelson B, Spiering S, Haverslag R, Kim C, Talantova M, Bajpai R, Calzolari D, Terskikh A, McCulloch AD, Price JH, Conklin BR, Chen HS, Mercola M. PLoS ONE. 2009 . 4(4):e5046. 10.1371/journal.pone.0005046 PubMed 19352491 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS aMHC-eGFP-Rex-Neo 13870 bp DNA SYN DEFINITION aMHC-eGFP-Rex-Neo ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..13870 /organism="aMHC-eGFP-Rex-Neo" /mol_type="other DNA" promoter 62..90 /label="AmpR_promoter" gene 132..992 /label="Ampicillin" /gene="Ampicillin" CDS 132..992 /label="ORF frame 3" rep_origin 1147..1766 /label="pBR322_origin" promoter 2075..2104 /label="lac_promoter" misc_feature 2118..2140 /label="M13_pUC_rev_primer" promoter 2139..2157 /label="M13_reverse_primer" promoter 2174..2193 /label="T3_promoter" misc_feature 2448..2628 /label="truncHIV-1_3_LTR" misc_feature 2448..2628 /label="HIV-1_5_LTR" misc_feature 2736..2780 /label="HIV-1_psi_pack" CDS 3168..4331 /label="ORF frame 3" misc_feature 3290..3523 /label="RRE" misc_feature 4023..4038 /label="cPPT" misc_feature 4181..4197 /label="pBluescriptSK_primer" CDS complement(8677..9189) /label="ORF frame 2" CDS 9670..10389 /label="ORF frame 1" gene 9673..10386 /label="EGFP" /gene="EGFP" misc_feature complement(9715..9736) /label="EGFP_N_primer" misc_feature 9853..9882 /label="Y66 (EGFP)" misc_feature 10323..10344 /label="EGFP_C_primer" misc_feature 10407..10982 /label="WPRE" CDS 10495..11031 /label="ORF frame 1" gene 11735..12535 /label="NeoR/KanR" /gene="NeoR/KanR" CDS 11735..12538 /label="ORF frame 2" misc_feature 12594..12609 /label="cPPT" misc_feature 12594..12615 /label="U3PPT" misc_feature 12611..12663 /label="delta_U3" misc_feature 12664..12844 /label="truncHIV-1_3_LTR" misc_feature 12664..12844 /label="HIV-1_5_LTR" terminator 12919..13038 /label="SV40_PA_terminator" misc_feature 13007..13026 /label="EBV_rev_primer" rep_origin 13056..13133 /label="SV40_origin" misc_feature 13118..13137 /label="SV40pro_F_primer" promoter complement(13224..13242) /label="T7_promoter" promoter complement(13252..13268) /label="M13_forward20_primer" misc_feature complement(13261..13283) /label="M13_pUC_fwd_primer" misc_feature 13261..13404 /label="lacZ_a" rep_origin 13430..13736 /label="f1_origin" ORIGIN 1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt 2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat 2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc 2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg 2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc 2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt 2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga 2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag 2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag 2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca 2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac 2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga 3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga 3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac 3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt 3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca 3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt 3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg 3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta 3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa 3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct 3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc 3601 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca 3661 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag 3721 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc 3781 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt 3841 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga 3901 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag 3961 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt 4021 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag 4081 caacagacat acaaacaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 4141 tcacgagact agcctcgagc tccaccgcgg tggcggccgc tctagaacta gtggatcctg 4201 caaggtcaca caagggtctc cacccaccag gtgccctagt ctcaatttca gtttccatgc 4261 cttgttctca caatgctggc ctccccagag ctaatttgga ctttgttttt atttcaaaag 4321 ggcctgaatg aggagtagat cttgtgctac ccagctctaa gggtgcccgt gaagccctca 4381 gacctggagc ctttgcaaca gccctttagg tggaagcaga ataaagcaat tttccttaaa 4441 gccaaaatcc tgcctctaga ctcttcttct ctgacctcgg tccctgggct ctagggtggg 4501 gaggtggggc ttggaagaag aaggtgggga agtggcaaaa gccgatccct agggccctgt 4561 gaagttcgga gccttccctg tacagcactg gctcatagat cctcctccag ccaaacatag 4621 caagaagtga tacctccttt gtgacttccc caggcccagt acctgtcagg ttgaaacagg 4681 atttagagaa gcctctgaac tcacctgaac tctgaagctc atccaccaag caagcaccta 4741 ggtgccactg ctagttagta tcctacgctg ataatatgca gagctgggcc acagaagtcc 4801 tggggtgtag gaactgacca gtgacttttc agtcggcaaa ggtatgaccc cctcagcaga 4861 tgtagtaatg tccccttaga tcccatccca ggcaggtctc taagaggaca tgggatgaga 4921 gatgtagtca tgtggcattc caaacacagc tatccacagt gtcccttgcc ccttccactt 4981 agccaggagg acagtaacct tagcctatct ttcttcctcc ccatcctccc aggacacacc 5041 ccctggtctg cagtattcat ttcttccttc acgtcccctc tgtgacttcc atttgcaagg 5101 cttttgacct ctgcagctgc tggaagatag agtttggccc taggtgtggc aagccatctc 5161 aagagaaagc agacaacagg gggaccagat tttggaagga tcaggaacta aatcactggc 5221 gggcctgggg gtagaaaaaa gagtgagtga gtccgctcca gctaagccaa gctagtcccc 5281 gagatactct gccacagctg ggctgctcgg ggtagcttta ggaatgtggg tctgaaagac 5341 aatgggattg gaagacatct ctttgagtct cccctcaacc ccacctacag acacactcgt 5401 gtgtggccag actcctgttc aacagccctc tgtgttctga ccactgagct aggcaaccag 5461 agcatgggcc ctgtgctgag gatgaagagt tggttaccaa tagcaaaaac agcaggggag 5521 ggagaacaga gaacgaaata aggaaggaag aaggaaaggc cagtcaatca gatgcagtca 5581 gaagagatgg gaagccaaca cacagcttga gcagaggaaa cagaaaaggg agagattctg 5641 ggcataagga ggccacagaa agaagagccc aggcccccca agtctcctct ttataccctc 5701 atcccgtctc ccaattaagc ccactcttct tcctagatca gacctgagct gcagcgaaga 5761 gacccgtagg gaggatcaca ctggatgaag gagatgtgtg gagaagtcca gggcaaccta 5821 agagccagag cctaaaagag caagagataa aggtgcttca aaggtggcca ggctgtgcac 5881 acagagggtc gaggactggt ggtagagcct caagataagg atgatgctca gaatgggcgg 5941 ggggggggat tctggggggg ggagagagaa ggtgagaagg agcctggaac agagaatctg 6001 gaagcgctgg aaacgatacc ataaagggaa gaacccaggc tacctttaga tgtaaatcat 6061 gaaagacagg gagaagggaa gctggagaga gtagaaggac cccggggcaa gacatggaag 6121 caaggacaag ccaggttgag cgctccgtga aatcagcctg ctgaaggcag agccctggta 6181 tgagcaccag aacagcagag gctagggtta atgtcgagac agggaacaga aggtagacac 6241 aggaacagac agagacgggg gagccaggta acaaaggaat ggtccttctc acctgtggcc 6301 agagcgtcca tctgtgtcca catactctag aatgttcatc agactgcagg gctggcttgg 6361 gaggcagctg gaaagagtat gtgagagcca ggggagacaa gggggcctag gaaaggaaga 6421 agagggcaaa ccaggccaca caagagggca gagcccagaa ctgagttaac tccttccttg 6481 ttgcatcttc cataggaggc agtgggaact ctgtgaccac catcccccat gagcccccac 6541 tacccatacc aagtttggcc tgagtggcat tctaggttcc ctgaggacag agcctggcct 6601 ttgtctcttg gacctgaccc aagctgaccc aatgttctca gtaccttatc atgccctcaa 6661 gagcttgaga accaggcagt gacatattag gccatgggct aaccctggag cttgcacaca 6721 ggagcctcaa gtgacctcca gggacacagc tgcagacagg tggcctttat ccccaaagag 6781 caaccatttg gcataggtgg ctgcaaatgg gaatgcaagg ttgaatcagg tcccttcaag 6841 aatactgcat gcaagaccta agacccctgg agagaggggt atgctcctgc ccccacccac 6901 cataagggga gtgaactatc ctagggggct ggcgaccttg gggagacacc acattactga 6961 gagtgctgag cccagaaaaa ctgaccgccc tgtgtcctgc ccacctccac actctagagc 7021 tatattgaga ggtgacagta gatagggtgg gagctggtag cagggagagt gttcctgggt 7081 gtgagggtgt aggggaaagc cagagcaggg gagtctggct ttgtctcctg aacacaatgt 7141 ctacttagtt ataacaggca tgacctgcta aagacccaac atctacgacc tctgaaaaga 7201 cagcagccct ggaggacagg ggttgtctct gagccttggg tgcttgatgg tgccacaaag 7261 gagggcatga gtgtgagtat aaggccccag gagcgttaga gaagggcact tgggaagggg 7321 tcagtctgca gagcccctat ccatggaatc tggagcctgg ggccaactgg tgtaaatctc 7381 tgggcctgcc aggcattcaa agcagcacct gcatcctctg gcagcctggg gaggcggaag 7441 ggagcaaccc cccacttata ccctttctcc ctcagcccca ggattaacac ctctggcctt 7501 cccccttccc acctcccatc aggagtggag ggttgcagag ggagggtaaa aacctacatg 7561 tccaaacatc atggtgcacg atatatggat cagtatgtgt agaggcaaga aaggaaatct 7621 gcaggcttaa ctgggttaat gtgtaaagtc tgtgtgcatg tgtgtgtgtc tgactgaaaa 7681 cgggcatggc tgtgcagctg ttcagttctg tgcgtgaggt taccagactg caggtttgtg 7741 tgtaaattgc ccaaggcaaa gtgggtgaat cccttccatg gtttaaagag attggatgat 7801 ggcctgcatc tcaaggacca tggaaaatag aatggacact ctatatgtgt ctctaagcta 7861 aggtagcaag gtctttggag gacacctgtc tagagatgtg ggcaacagag actacagaca 7921 gtatctgtac agagtaagga gagagaggag ggggtgtaga attctcttac tatcaaaggg 7981 aaactgagtc gtgcacctgc aaagtggatg ctctccctag acatcatgac tttgtctctg 8041 gggagccagc actgtggaac ttcaggtctg agagagtagg aggctcccct cagcctgaag 8101 ctatgcagat agccagggtt gaaaggggga agggagagcc tgggatggga gcttgtgtgt 8161 tggaggcagg ggacagatat taagcctgga agagaaggtg acccttaccc agttgttcaa 8221 ctcacccttc agattaaaaa taactgaggt aagggcctgg gtaggggagg tggtgtgaga 8281 cgctcctgtc tctcctctat ctgcccatcg gccctttggg gaggaggaat gtgcccaagg 8341 actaaaaaaa ggccatggag ccagaggggc gagggcaaca gacctttcat gggcaaacct 8401 tggggccctg ctgtcctcct gtcacctcca gagccaaggg atcaaaggag gaggagccag 8461 gacaggaggg aagtgggagg gagggtccca gcagaggact ccaaatttag gcagcaggca 8521 tatgggatgg gatataaagg ggctggagca ctgagagctg tcagagattt ctccaaccca 8581 ggtaagaggg agtttcgggt gggggctctt cacccacacc agacctctcc ccacctagaa 8641 ggaaactgcc tttcctggaa gtggggttca ggccggtcag agatctgaca gggtggcctt 8701 ccaccagcct gggaagttct cagtggcagg aggtttccac aagaaacact ggatgcccct 8761 tcccttacgc tgtcttctcc atcttcctcc tggggatgct cctccccgtc ttggtttatc 8821 ttggctcttc gtcttcagca agatttgccc tgtgctgtcc actccatctt tctctactgt 8881 ctccgtgcct tgccttgcct tcttgcgtgt ccttcctttc cacccatttc tcacttcacc 8941 ttttctcccc ttctcatttg tattcatcct tccttccttc cttccttcct tccttccttc 9001 cttccttcct tcctttctcc cttccttcct tccttccttc cttccttcct tccttccttc 9061 ctgtgtcaga gtgctgagaa tcacacctgg ggttcccacc cttatgtaaa caatcttcca 9121 gtgagccaca gcttcagtgc tgctgggtgc tctcttacct tcctcacccc ctggcttgtc 9181 ctgttccatc ctggtcagga tctctagatt ggtctcccag cctctgctac tcctcttcct 9241 gcctgttcct ctctctgtcc agctgcgcca ctgtggtgcc tcgttccagc tgtggtccac 9301 attcttcagg attctctgaa aagttaacca ggtgagaatg tttcccctgt agacagcaga 9361 tcacgattct cccggaagtc aggcttccag ccctctcttt ctctgcccag ctgcccggca 9421 ctcttagcaa acctcaggca cccttacccc acatagacct ctgacagaga agcaggcact 9481 ttacatggag tcctggtggg agagccatag gctacggtgt aaaagaggca gggaagtggt 9541 ggtgtaggaa agtcaggact tcacatagaa gcctagccca caccagaaat gacagacaga 9601 tccctcctat ctcccccata agagtttgag tcgacggtac cgcgggcccg ggatccaccg 9661 gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 9721 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat 9781 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc 9841 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac 9901 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc 9961 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc 10021 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc 10081 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag 10141 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg 10201 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc 10261 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat 10321 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg 10381 tacaagtaaa gcggccgcgt cgacaatcaa cctctggatt acaaaatttg tgaaagattg 10441 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct 10501 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg 10561 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact 10621 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc 10681 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc 10741 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag 10801 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc 10861 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg 10921 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg 10981 gccgcctccc cgcctgaatt tcaccggcga ccgattcctc ccgataagta aattacagaa 11041 aagcttcagg ttaaatgtaa gaggatctta aatattgatt tggtttttgt cttaatggtg 11101 cccggcgtat ttataagaac cattaaattt cactagaccg tttgtagtca ggggtgaacg 11161 ctgcatgacc cacgctctca aagtgagttg tgttgctttg cgggaatcca gcagtcacat 11221 taatgctgga aaaagttcag gcaactagtg tactttgtag cggggtccgg gagaggctgg 11281 ggtctagagt ggcgatggga cgaaagggta aagaaagttt tcggtgtgtg tgtgtgtgtg 11341 tgtgtgtgtg tgtgtgtgtg tgtgtgtgta ggtaggtagg taggtagaaa ctgcatcctc 11401 tgcttgtgta aatccggtta catgtgtgta acagaggtac tgagatgtga ctgagtctca 11461 aggccaggcg atcgggattc agaagaggca tttgcataac tgagcaagag cctttgcccc 11521 acccttccac gcggacccaa gacaagcgga cagctctggg tgggtcacct tgaagccagg 11581 ggcccgccca catccccgcc cacacccacc ttgagcgctt ctcattgggc attccagcct 11641 acccagctcg gagttagtta ctccgtaagt gtggccggaa cagagttcgt ccatctaaaa 11701 agggagggga ccggcgaaca catctagagc caccatggga tcggccattg aacaagatgg 11761 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca 11821 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt 11881 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg 11941 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga 12001 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca 12061 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct 12121 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac 12181 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc 12241 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgatg atctcgtcgt 12301 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt 12361 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg 12421 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat 12481 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgaag 12541 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa 12601 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt 12661 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 12721 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 12781 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 12841 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata 12901 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc 12961 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 13021 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt 13081 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg 13141 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt 13201 gcgtcgagac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt 13261 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc 13321 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca 13381 acagttgcgc agcctgaatg gcgaatggcg cgacgcgccc tgtagcggcg cattaagcgc 13441 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc 13501 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct 13561 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa 13621 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc 13681 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact 13741 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg 13801 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt 13861 tacaatttcc //
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