载体名称: | pJP1 |
---|---|
来源历史: | <- J.M. Pemberton |
载体大小: | 88 ± 3 kb |
质粒抗性: |
2,4-D+
|
载体说明: | 土壤分离物;Inc P-1;对雄性特异性噬菌体PR11敏感;编码2,4-二氯苯氧乙酸、2-甲基、4-氯、苯氧乙酸和达拉彭、2,2-二氯丙酸的降解;2,4-D+特征在该菌株中稳定保留,该菌株使用2,4-D作为唯一的碳源和能量源;菌株不降解苯氧乙酸。 |
宿主菌: | Variovorax paradoxus (former: Alcaligenes paradoxus) JMP116 |
培养条件: | LB培养基,28度 |
参考文献: |
Pemberton, J. M., Fischer, P. R. (1977). 2,4-D plasmids and persistance. Nature 268 : 732-733 . Pemberton, J. M., Corney, B., Don, R. H., (1979). Evolution and spread of pesticide degrading ability among soil micro-organisms. In Plasmids of medical, environmental and commercial importance, Proceedings of a symposium held in Spitzingsee april 26-28, 1979. Elsevier and North Holland Biomedical Press , pp. 287-299. Edited by K. N. Timmis, Elsevier/North-Holland Biomedical Press. Fisher, P. R., Appleton, J., Pemberton, J. M. (1978). Isolation and characterization of the pesticide-degrading plasmid pJP1 from Alcaligenes paradoxus. J.Bacteriol. 135 : 798-804 . |
安全等级: | 1级 |
分离国家: | 原产国未知 |
存储时间: | 1990年2月23日前 |