| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pLenti6/TR |
| 质粒类型: | Gateway系统；慢病毒表达载体；四环素调控载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | CMV |
| 克隆方法: | -- |
| 载体大小: | 8355 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | Ampicillin |
| 筛选标记: | Blasticidin |
| 备注: | pLenti6/TR是四环素调控系统的调控载体，对应的应答载体为pLenti4/TO/V5-Dest。 |
| 产品目录号: | V480-20 |
| 稳定性: | 稳表达 |
| 组成型: | 组成型 |
| 病毒/非病毒: | 慢病毒 |
买家导航
pLenti6/TR载体质粒基本信息
pLenti6/TR质粒图谱载体图谱和pLenti6/TR载体序列质粒序列多克隆位点信息
pLenti6/TR质粒载体简介
pLenti6/TR质粒序列载体序列
LOCUS pLenti6/TR 8354 bp DNA SYN
DEFINITION pLenti6/TR
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..8354
/organism="pLenti6/TR"
/mol_type="other DNA"
misc_feature 230..410
/label="HIV-1_5_LTR"
misc_feature 230..410
/label="truncHIV-1_3_LTR"
misc_feature 521..565
/label="HIV-1_psi_pack"
CDS 953..1801
/label="ORF frame 2"
misc_feature 1075..1308
/label="RRE"
promoter 1813..2317
/label="CMV_immearly_promoter"
misc_feature 1820..2107
/label="CAG_enhancer"
misc_feature 2274..2294
/label="CMV_fwd_primer"
promoter 2275..2344
/label="CMV_promoter"
misc_feature 2318..2337
/label="pCEP_fwd_primer"
misc_feature 2320..2344
/label="LNCX_primer"
misc_feature 2552..3124
/label="bGlob_int"
misc_feature 3132..3151
/label="pCAG_F_primer"
promoter 3179..3197
/label="T7_promoter"
CDS 3208..3855
/label="ORF frame 1"
misc_feature 3211..3822
/label="TetR"
misc_feature complement(3942..3962)
/label="pBABE_3_primer"
misc_feature complement(3948..4163)
/label="SV40_enhancer"
promoter 3960..4228
/label="SV40_promoter"
rep_origin 4127..4204
/label="SV40_origin"
misc_feature 4189..4208
/label="SV40pro_F_primer"
promoter 4322..4389
/label="EM7_promoter"
gene 4390..4788
/label="blast"
/gene="blast"
misc_feature 4858..4873
/label="cPPT"
misc_feature 4858..4879
/label="U3PPT"
misc_feature 4875..4927
/label="delta_U3"
misc_feature 4928..5108
/label="truncHIV-1_3_LTR"
misc_feature 4928..5108
/label="HIV-1_5_LTR"
terminator 5183..5302
/label="SV40_PA_terminator"
misc_feature 5271..5290
/label="EBV_rev_primer"
rep_origin 5320..5418
/label="SV40_origin"
misc_feature 5403..5422
/label="SV40pro_F_primer"
promoter complement(5497..5515)
/label="T7_promoter"
promoter complement(5525..5541)
/label="M13_forward20_primer"
misc_feature complement(5534..5556)
/label="M13_pUC_fwd_primer"
misc_feature 5534..5682
/label="lacZ_a"
rep_origin 5700..6006
/label="f1_origin"
promoter 6199..6227
/label="AmpR_promoter"
gene 6269..7129
/label="Ampicillin"
/gene="Ampicillin"
CDS 6269..7129
/label="ORF frame 2"
rep_origin 7284..7903
/label="pBR322_origin"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
promoter 8212..8241
/label="lac_promoter"
misc_feature 8255..8277
/label="M13_pUC_rev_primer"
promoter 8276..8294
/label="M13_reverse_primer"
promoter 8311..8330
/label="T3_promoter"
ORIGIN
1 AATGTAGTCT TATGCAATAC TCTTGTAGTC TTGCAACATG GTAACGATGA GTTAGCAACA
61 TGCCTTACAA GGAGAGAAAA AGCACCGTGC ATGCCGATTG GTGGAAGTAA GGTGGTACGA
121 TCGTGCCTTA TTAGGAAGGC AACAGACGGG TCTGACATGG ATTGGACGAA CCACTGAATT
181 GCCGCATTGC AGAGATATTG TATTTAAGTG CCTAGCTCGA TACATAAACG GGTCTCTCTG
241 GTTAGACCAG ATCTGAGCCT GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC
301 TCAATAAAGC TTGCCTTGAG TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT GTGACTCTGG
361 TAACTAGAGA TCCCTCAGAC CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA GTGGCGCCCG
421 AACAGGGACT TGAAAGCGAA AGGGAAACCA GAGGAGCTCT CTCGACGCAG GACTCGGCTT
481 GCTGAAGCGC GCACGGCAAG AGGCGAGGGG CGGCGACTGG TGAGTACGCC AAAAATTTTG
541 ACTAGCGGAG GCTAGAAGGA GAGAGATGGG TGCGAGAGCG TCAGTATTAA GCGGGGGAGA
601 ATTAGATCGC GATGGGAAAA AATTCGGTTA AGGCCAGGGG GAAAGAAAAA ATATAAATTA
661 AAACATATAG TATGGGCAAG CAGGGAGCTA GAACGATTCG CAGTTAATCC TGGCCTGTTA
721 GAAACATCAG AAGGCTGTAG ACAAATACTG GGACAGCTAC AACCATCCCT TCAGACAGGA
781 TCAGAAGAAC TTAGATCATT ATATAATACA GTAGCAACCC TCTATTGTGT GCATCAAAGG
841 ATAGAGATAA AAGACACCAA GGAAGCTTTA GACAAGATAG AGGAAGAGCA AAACAAAAGT
901 AAGACCACCG CACAGCAAGC GGCCGCTGAT CTTCAGACCT GGAGGAGGAG ATATGAGGGA
961 CAATTGGAGA AGTGAATTAT ATAAATATAA AGTAGTAAAA ATTGAACCAT TAGGAGTAGC
1021 ACCCACCAAG GCAAAGAGAA GAGTGGTGCA GAGAGAAAAA AGAGCAGTGG GAATAGGAGC
1081 TTTGTTCCTT GGGTTCTTGG GAGCAGCAGG AAGCACTATG GGCGCAGCGT CAATGACGCT
1141 GACGGTACAG GCCAGACAAT TATTGTCTGG TATAGTGCAG CAGCAGAACA ATTTGCTGAG
1201 GGCTATTGAG GCGCAACAGC ATCTGTTGCA ACTCACAGTC TGGGGCATCA AGCAGCTCCA
1261 GGCAAGAATC CTGGCTGTGG AAAGATACCT AAAGGATCAA CAGCTCCTGG GGATTTGGGG
1321 TTGCTCTGGA AAACTCATTT GCACCACTGC TGTGCCTTGG AATGCTAGTT GGAGTAATAA
1381 ATCTCTGGAA CAGATTTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA
1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA
1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA
1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT
1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT
1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG
1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGATA
1801 AGCTTGGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA
1861 CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC
1921 TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA
1981 AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG
2041 GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT
2101 AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG
2161 GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG
2221 GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT
2281 GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCGTTTAG TGAACCGTCA
2341 GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGACACC GACTCTAGAG
2401 GATCCACTAG TCCAGTGTGG TGGAATTCTG CAGATAGCTT GGTACCCGGG GATCCTCTAG
2461 GGCCTCTGAG CTATTCCAGA AGTAGTGAAG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
2521 AAAGCTCCGG ATCGATCCTG AGAACTTCAG GGTGAGTTTG GGGACCCTTG ATTGTTCTTT
2581 CTTTTTCGCT ATTGTAAAAT TCATGTTATA TGGAGGGGGC AAAGTTTTCA GGGTGTTGTT
2641 TAGAATGGGA AGATGTCCCT TGTATCACCA TGGACCCTCA TGATAATTTT GTTTCTTTCA
2701 CTTTCTACTC TGTTGACAAC CATTGTCTCC TCTTATTTTC TTTTCATTTT CTGTAACTTT
2761 TTCGTTAAAC TTTAGCTTGC ATTTGTAACG AATTTTTAAA TTCACTTTTG TTTATTTGTC
2821 AGATTGTAAG TACTTTCTCT AATCACTTTT TTTTCAAGGC AATCAGGGTA TATTATATTG
2881 TACTTCAGCA CAGTTTTAGA GAACAATTGT TATAATTAAA TGATAAGGTA GAATATTTCT
2941 GCATATAAAT TCTGGCTGGC GTGGAAATAT TCTTATTGGT AGAAACAACT ACATCCTGGT
3001 CATCATCCTG CCTTTCTCTT TATGGTTACA ATGATATACA CTGTTTGAGA TGAGGATAAA
3061 ATACTCTGAG TCCAAACCGG GCCCCTCTGC TAACCATGTT CATGCCTTCT TCTTTTTCCT
3121 ACAGCTCCTG GGCAACGTGC TGGTTATTGT GCTGTCTCAT CATTTTGGCA AAGAATTGTA
3181 ATACGACTCA CTATAGGGCG AATTGATATG TCTAGATTAG ATAAAAGTAA AGTGATTAAC
3241 AGCGCATTAG AGCTGCTTAA TGAGGTCGGA ATCGAAGGTT TAACAACCCG TAAACTCGCC
3301 CAGAAGCTAG GTGTAGAGCA GCCTACATTG TATTGGCATG TAAAAAATAA GCGGGCTTTG
3361 CTCGACGCCT TAGCCATTGA GATGTTAGAT AGGCACCATA CTCACTTTTG CCCTTTAGAA
3421 GGGGAAAGCT GGCAAGATTT TTTACGTAAT AACGCTAAAA GTTTTAGATG TGCTTTACTA
3481 AGTCATCGCG ATGGAGCAAA AGTACATTTA GGTACACGGC CTACAGAAAA ACAGTATGAA
3541 ACTCTCGAAA ATCAATTAGC CTTTTTATGC CAACAAGGTT TTTCACTAGA GAATGCATTA
3601 TATGCACTCA GCGCTGTGGG GCATTTTACT TTAGGTTGCG TATTGGAAGA TCAAGAGCAT
3661 CAAGTCGCTA AAGAAGAAAG GGAAACACCT ACTACTGATA GTATGCCGCC ATTATTACGA
3721 CAAGCTATCG AATTATTTGA TCACCAAGGT GCAGAGCCAG CCTTCTTATT CGGCCTTGAA
3781 TTGATCATAT GCGGATTAGA AAAACAACTT AAATGTGAAA GTGGGTCCGC GTACAGCGGA
3841 TCCCGGGAAT TCTAGAGGGC CCGCGGTTCG AACAAAAACT CATCTCAGAA GAGGATCTGA
3901 ATATGCATAC CGGTTAGTAA TGAGTTTGGA ATTAATTCTG TGGAATGTGT GTCAGTTAGG
3961 GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA
4021 GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT
4081 GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC
4141 TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA
4201 GGCCGAGGCC GCCTCTGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG
4261 CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAGCA
4321 CGTGTTGACA ATTAATCATC GGCATAGTAT ATCGGCATAG TATAATACGA CAAGGTGAGG
4381 AACTAAACCA TGGCCAAGCC TTTGTCTCAA GAAGAATCCA CCCTCATTGA AAGAGCAACG
4441 GCTACAATCA ACAGCATCCC CATCTCTGAA GACTACAGCG TCGCCAGCGC AGCTCTCTCT
4501 AGCGACGGCC GCATCTTCAC TGGTGTCAAT GTATATCATT TTACTGGGGG ACCTTGTGCA
4561 GAACTCGTGG TGCTGGGCAC TGCTGCTGCT GCGGCAGCTG GCAACCTGAC TTGTATCGTC
4621 GCGATCGGAA ATGAGAACAG GGGCATCTTG AGCCCCTGCG GACGGTGCCG ACAGGTGCTT
4681 CTCGATCTGC ATCCTGGGAT CAAAGCCATA GTGAAGGACA GTGATGGACA GCCGACGGCA
4741 GTTGGGATTC GTGAATTGCT GCCCTCTGGT TATGTGTGGG AGGGCTAAGC ACAATTCGAG
4801 CTCGGTACCT TTAAGACCAA TGACTTACAA GGCAGCTGTA GATCTTAGCC ACTTTTTAAA
4861 AGAAAAGGGG GGACTGGAAG GGCTAATTCA CTCCCAACGA AGACAAGATC TGCTTTTTGC
4921 TTGTACTGGG TCTCTCTGGT TAGACCAGAT CTGAGCCTGG GAGCTCTCTG GCTAACTAGG
4981 GAACCCACTG CTTAAGCCTC AATAAAGCTT GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG
5041 TCTGTTGTGT GACTCTGGTA ACTAGAGATC CCTCAGACCC TTTTAGTCAG TGTGGAAAAT
5101 CTCTAGCAGT AGTAGTTCAT GTCATCTTAT TATTCAGTAT TTATAACTTG CAAAGAAATG
5161 AATATCAGAG AGTGAGAGGA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA
5221 TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC
5281 CAAACTCATC AATGTATCTT ATCATGTCTG GCTCTAGCTA TCCCGCCCCT AACTCCGCCC
5341 ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT
5401 TTTATTTATG CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA
5461 GGCTTTTTTG GAGGCCTAGG GACGTACCCA ATTCGCCCTA TAGTGAGTCG TATTACGCGC
5521 GCTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA
5581 ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG
5641 ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG GGACGCGCCC TGTAGCGGCG
5701 CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC
5761 TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC
5821 GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG
5881 ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG
5941 TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG
6001 GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT
6061 CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTTAACAAAA
6121 TATTAACGCT TACAATTTAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG
6181 TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT
6241 GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT
6301 TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT
6361 AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG
6421 CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA
6481 AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG
6541 CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT
6601 TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC
6661 TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA
6721 CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT
6781 ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT
6841 ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC
6901 GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA
6961 TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG
7021 TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG
7081 AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA
7141 AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA
7201 GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA
7261 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG
7321 CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA
7381 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA
7441 TACTGTTCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC
7501 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG
7561 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC
7621 GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT
7681 ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC
7741 GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG
7801 GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG
7861 CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT
7921 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA
7981 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG
8041 CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC
8101 GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG
8161 TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT
8221 TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA
8281 CAGCTATGAC CATGATTACG CCAAGCGCGC AATTAACCCT CACTAAAGGG AACAAAAGCT
8341 GGAGCTGCAA GCTT
//
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pLenti6/TR质粒载体应用举例
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