| 质粒名称: | pOsU3 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 170132 |
| 存储实验室: | Caixia Gao |
|
载体骨架基本信息 |
|
| 载体名称: | pUC (BsaI blocked) |
| 载体出品公司: | Clontech |
| 完整质粒大小: | 3195bp |
| 载体类型: | Plant Expression, CRISPR |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | |
|
文献引用方法 |
|
| 材料和方法部分: | pOsU3 was a gift from Caixia Gao (Addgene plasmid # 170132 ; http://n2t.net/addgene:170132 ; RRID:Addgene_170132) |
| 参考文献部分: |
pOsU3 was a gift from Caixia Gao (Addgene plasmid # 170132 ; http://n2t.net/addgene:170132 ; RRID:Addgene_170132)
For your References section:
High-efficiency prime editing with optimized, paired pegRNAs in plants. Lin Q, Jin S, Zong Y, Yu H, Zhu Z, Liu G, Kou L, Wang Y, Qiu JL, Li J, Gao C. Nat Biotechnol. 2021 Mar 25. pii: 10.1038/s41587-021-00868-w. doi: 10.1038/s41587-021-00868-w. 10.1038/s41587-021-00868-w PubMed 33767395
|
买家导航
pOsU3载体质粒基本信息
pOsU3质粒图谱载体图谱和pOsU3载体序列质粒序列多克隆位点信息
pOsU3质粒载体简介
pOsU3质粒序列载体序列
LOCUS pOsU3 3195 bp DNA circular SYN 25-AUG-2024
DEFINITION For sgRNA expression in monocotyledons protoplasts.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3195)
AUTHORS Lin Q, Jin S, Zong Y, Yu H, Zhu Z, Liu G, Kou L, Wang Y, Qiu JL, Li
J, Gao C
TITLE High-efficiency prime editing with optimized, paired pegRNAs in
plants.
JOURNAL Nat Biotechnol. 2021 Mar 25. pii: 10.1038/s41587-021-00868-w. doi:
10.1038/s41587-021-00868-w.
PUBMED 33767395
REFERENCE 2 (bases 1 to 3195)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat
Biotechnol. 2021 Mar 25. pii: 10.1038/s41587-021-00868-w. doi:
10.1038/s41587-021-00868-w."
FEATURES Location/Qualifiers
source 1..3195
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(19..41)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 141..160
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 354..376
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 368..385
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 369..385
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 449..828
/label=OsU3 promoter
/note="Oryza sativa (rice) snRNA U3 promoter"
misc_RNA 850..925
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
terminator 926..945
/label=SUP4 terminator
/note="transcription terminator for the S. cerevisiae SUP4
tRNA gene"
primer_bind complement(964..980)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(964..980)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(977..999)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 988..1004
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(1012..1042)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 1057..1078
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(1195..1212)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(1366..1954)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(1446..1465)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(2125..2985)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 2748..2767
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(2986..3090)
/gene="bla"
/label=AmpR promoter
primer_bind 3158..3176
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
61 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
121 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
181 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt
241 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
301 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
361 acgacgttgt aaaacgacgg ccagtgaatt cagtaattca tccaggtcac caagttctag
421 gattttcaga actgcaactt attttatcaa ggaatcttta aacatacgaa cagatcactt
481 aaagttcttc tgaagcaact taaagttatc aggcatgcat ggatcttgga ggaatcagat
541 gtgcagtcag ggaccatagc acaagacagg cgtcttctac tggtgctacc agcaaatgct
601 ggaagccggg aacactgggt acgttggaaa ccacgtgatg tgaagaagta agataaactg
661 taggagaaaa gcatttcgta gtgggccatg aagcctttca ggacatgtat tgcagtatgg
721 gccggcccat tacgcaattg gacgacaaca aagactagta ttagtaccac ctcggctatc
781 cacatagatc aaagctgatt taaaagagtt gtgcagatga tccgtggcgt gagaccctgc
841 agggtctctg ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact
901 tgaaaaagtg gcaccgagtc ggtgcttttt ttgtttttta tgtctaagct tggcgtaatc
961 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
1021 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
1081 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
1141 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
1201 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
1261 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
1321 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
1381 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
1441 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
1501 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
1561 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
1621 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
1681 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
1741 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
1801 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
1861 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
1921 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
1981 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
2041 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
2101 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
2161 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
2221 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagaac cacgctcacc
2281 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
2341 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
2401 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
2461 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
2521 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
2581 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
2641 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
2701 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
2761 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
2821 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
2881 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
2941 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
3001 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
3061 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
3121 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
3181 tcgtctcgcg cgttt
//
pOsU3载体图谱质粒图谱pdf版和pOsU3载体序列质粒序列等相关资料下载
pOsU3质粒载体应用举例
- 上一篇：pMOD_C0000
- 下一篇：没有了
