pMV306hsp+LuxG13

LOCUS       pMV306hsp+LuxG13               10550 bp ds-DNA     circular SYN 10-JAN-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pMV306hsp+LuxG13
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10550)
  AUTHORS   Andreu N, Zelmer A, Fletcher T, Elkington PT, Ward TH, Ripoll J, 
            Parish T, Bancroft GJ, Schaible U, Robertson BD, Wiles S
  TITLE     Optimisation of bioluminescent reporters for use with mycobacteria.
  JOURNAL   PLoS One. 2010 May 24;5(5):e10777.
  PUBMED    20520722
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTNAME|pMV306hsp+LuxG13|
FEATURES             Location/Qualifiers
     source          1..10550
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(1896..1915)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1907..2722
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1980..1999)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3054..3642
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3543..3562
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             4321..5403
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             5418..6401
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVAIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMKYT"
     CDS             6888..8330
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPESDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MRHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             8342..9265
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PDNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             9291..10403
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     terminator      10468..10514
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gctagagccg tgaacgacag ggcgaacgcc agcccgccga cggcgagggt tccgaccgct
       61 gcaactcccg gtgcaacctt gtcccggtct attctcttca ctgcaccagc tccaatctgg
      121 tgtgaatgcc cctcgtctgt tcgcgcaggc ggggggctct attcgtttgt cagcatcgaa
      181 agtagccaga tcagggatgc gttgcaaccg cgtatgccca ggtcagaaga gtcgcacaag
      241 agttgcagac ccctggaaag aaaaatggcc agagggcgaa aacaccctct gaccagcgga
      301 gcgggcgacg ggaatcgaac ccgcgtagct agtttggaag aatgggtgtc tgccgaccac
      361 atatatgggc cggtcaagat aggtttttac cccctctcgg ctgcatcctc taagtggaaa
      421 gaaattgcag gtcgtagaag cgcgttgaag cctgagagtt gcacaggagt tgcaacccgg
      481 tagccttgtt cacgacgaga ggagacctag ttggcacgtc gcggatgggg atcgctgaag
      541 actcagcgca gcgggaggat ccaagcctca tacgtcaacc cgcaggacgg tgtgaggtac
      601 tacgcgctgc agacctacga caacaagatg gacgccgaag cctggctcgc gggcgagaag
      661 cggctcatcg agatggagac ctggacccct ccacaggacc gggcgaagaa ggcagccgcc
      721 agcgccatca cgctggagga gtacacccgg aagtggctcg tggagcgcga cctcgcagac
      781 ggcaccaggg atctgtacag cgggcacgcg gagcgccgca tctacccggt gctaggtgaa
      841 gtggcggtca cagagatgac gccagctctg gtgcgtgcgt ggtgggccgg gatgggtagg
      901 aagcacccga ctgcccgccg gcatgcctac aacgtcctcc gggcggtgat gaacacagcg
      961 gtcgaggaca agctgatcgc agagaacccg tgccggatcg agcagaaggc agccgatgag
     1021 cgcgacgtag aggcgctgac gcctgaggag ctggacatcg tcgccgctga gatcttcgag
     1081 cactaccgga tcgcggcata catcctggcg tggacgagcc tccggttcgg agagctgatc
     1141 gagcttcgcc gcaaggacat cgtggacgac ggcatgacga tgaagctccg ggtgcgccgt
     1201 ggcgcttccc gcgtggggaa caagatcgtc gttggcaacg ccaagaccgt ccggtcgaag
     1261 cgtcctgtga cggttccgcc tcacgtcgcg gagatgatcc gagcgcacat gaaggaccgt
     1321 acgaagatga acaagggccc cgaggcattc ctggtgacca cgacgcaggg caaccggctg
     1381 tcgaagtccg cgttcaccaa gtcgctgaag cgtggctacg ccaagatcgg tcggccggaa
     1441 ctccgcatcc acgacctccg cgctgtcggc gctacgttcg ccgctcaggc aggtgcgacg
     1501 accaaggagc tgatggcccg tctcggtcac acgactccta ggatggcgat gaagtaccag
     1561 atggcgtctg aggcccgcga cgaggctatc gctgaggcga tgtccaagct ggccaagacc
     1621 tcctgaaacg caaaaagccc ccctcccaag gacactgagt cctaaagagg ggggtttctt
     1681 gtcagtacgc gaagaaccac gcctggccgc gagcgccagc accgccgctc tgtgcggaga
     1741 cctgggcacc agccccgccg ccgccaggag cattgccgtt cccgccagct agcaacaaag
     1801 cgacgttgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     1861 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca
     1921 acgggaaacg tcttgctcga ggccgcgatt aaattccaac atggatgctg atttatatgg
     1981 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg
     2041 gaagccccat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
     2101 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
     2161 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccgggaaaac
     2221 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
     2281 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg
     2341 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
     2401 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataatct
     2461 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
     2521 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
     2581 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
     2641 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
     2701 gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat
     2761 tacgctgact tgacgggacg gcggctttgt tgaataaatc gaacttttgc tgagttgaag
     2821 gatcagatca cgcatcttcc cgacaacgca gaccgttccg tggcaaagca aaagttcaaa
     2881 atcaccaact ggtccaccta caacaaagct ctcatcaacc gtggctccct cactttctgg
     2941 ctggatgatg gggcgattca ggcctggtat gagtcagcaa caccttcttc acgaggcaga
     3001 cctcactagt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     3061 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     3121 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     3181 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     3241 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     3301 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     3361 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     3421 gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag
     3481 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3541 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3601 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3661 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3721 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3781 cgaacgaccg agcgcaacgc gtgcggccgc tctagaggtg accacaacga cgcgcccgct
     3841 ttgatcgggg acgtctgcgg ccgaccattt acgggtcttg ttgtcgttgg cggtcatggg
     3901 ccgaacatac tcacccggat cggagggccg aggacaaggt cgaacgaggg gcatgacccg
     3961 gtgcggggct tcttgcactc ggcataggcg agtgctaaga ataacgttgg cactcgcgac
     4021 cggtgagtcg taggtcggga cggtgaggcc aggcccgtcg tcgcagcgag tggcagcgag
     4081 gacaacttga gccgtccgtc gcgggcactg cgcccggcca gcgtaagtag cggggttgcc
     4141 gtcacccggt gacccccggt ttcatccccg atccggagga atcacttcgc aatggccaag
     4201 acaattgcgg atcccccggg ctgcaggaat tcgaatggcc atgggacgtc gacctgaggt
     4261 aattataacc cgggccctat atatggatcc aattgcaatg atcgtcgaca ggaggactct
     4321 atgaaatttg gaaacttttt gcttacatac caacctcccc aattttctca aacagaggta
     4381 atgaaacgtt tggttaaatt aggtcgcatc tctgaggagt gtggttttga taccgtatgg
     4441 ttactggagc atcatttcac ggagtttggt ttgcttggta acccttatgt cgctgctgca
     4501 tatttacttg gcgcgactaa aaaattgaat gtaggaactg ccgctattgt tcttcccaca
     4561 gcccatccag tacgccaact tgaagatgtg aatttattgg atcaaatgtc aaaaggacga
     4621 tttcggtttg gtatttgccg agggctttac aacaaggact ttcgcgtatt cggcacagat
     4681 atgaataaca gtcgcgcctt agcggaatgc tggtacgggc tgataaagaa tggcatgaca
     4741 gagggatata tggaagctga taatgaacat atcaagttcc ataaggtaaa agtaaacccc
     4801 gcggcgtata gcagaggtgg cgcaccggtt tatgtggtgg ctgaatcagc ttcgacgact
     4861 gagtgggctg ctcaatttgg cctaccgatg atattaagtt ggattataaa tactaacgaa
     4921 aagaaagcac aacttgagct ttataatgaa gtggctcaag aatatgggca cgatattcat
     4981 aatatcgacc attgcttatc atatataaca tctgtagatc atgactcaat taaagcgaaa
     5041 gagatttgcc ggaaatttct ggggcattgg tatgattctt atgtgaatgc tacgactatt
     5101 tttgatgatt cagaccaaac aagaggttat gatttcaata aagggcagtg gcgtgacttt
     5161 gtattaaaag gacataaaga tactaatcgc cgtattgatt acagttacga aatcaatccc
     5221 gtgggaacgc cgcaggaatg tattgacata attcaaaaag acattgatgc tacaggaata
     5281 tcaaatattt gttgtggatt tgaagctaat ggaacagtag acgaaattat tgcttccatg
     5341 aagctcttcc agtctgatgt catgccattt cttaaagaaa aacaacgttc gctattatat
     5401 tagctaagga gaaagaaatg aaatttggat tgttcttcct taacttcatc aattcaacaa
     5461 ctgttcaaga acaaagtata gttcgcatgc aggaaataac ggagtatgtt gataagttga
     5521 attttgaaca gattttagtg tatgaaaatc atttttcaga taatggtgtt gtcggcgctc
     5581 ctctgactgt ttctggtttt ctgctcggtt taacagagaa aattaaaatt ggttcattaa
     5641 atcacatcat tacaactcat catcctgtcg ccatagcgga ggaagcttgc ttattggatc
     5701 agttaagtga agggagattt attttagggt ttagtgattg cgaaaaaaaa gatgaaatgc
     5761 atttttttaa tcgcccggtt gaatatcaac agcaactatt tgaagagtgt tatgaaatca
     5821 ttaacgatgc tttaacaaca ggctattgta atccagataa cgatttttat agcttcccta
     5881 aaatatctgt aaatccccat gcttatacgc caggcggacc tcggaaatat gtaacagcaa
     5941 ccagtcatca tattgttgag tgggcggcca aaaaaggtat tcctctcatc tttaagtggg
     6001 atgattctaa tgatgttaga tatgaatatg ctgaaagata taaagccgtt gcggataaat
     6061 atgacgttga cctatcagag atagaccatc agttaatgat attagttaac tataacgaag
     6121 atagtaataa agctaaacaa gagacgcgtg catttattag tgattatgtt cttgaaatgc
     6181 accctaatga aaatttcgaa aataaacttg aagaaataat tgcagaaaac gctgtcggaa
     6241 attatacgga gtgtataact gcggctaagt tggcaattga aaagtgtggt gcgaaaagtg
     6301 tattgctgtc ctttgaacca atgaatgatt tgatgagcca aaaaaatgta atcaatattg
     6361 ttgatgataa tattaagaag taccacatga aatataccta ataggtaccg atcgccacta
     6421 gcgccgcggt cggaatcagc gcactggccg ccggagcggt cagctacctg ttgctgcgcc
     6481 gcttcgcttt ctagcaccga cgtcccggcg ccagcagcta cctctcgagc aaacgttttg
     6541 cgcgcccgaa aaataagtcg ttgcagaact ttcatgaatt aggccttgct gcgcccaggc
     6601 tccagtagta gaaatggagt cacggcagcc cggtgaagcc aaggtcgaac cggaagagaa
     6661 ggttcgtcct cccgacccgg gcacccagca cggcccccgg aacccacgcg gagtcatagc
     6721 cgcgataatg gcagaagtgt tgcgggcctg cgtaattgcg aaattcagat ggtgccgacg
     6781 gccctttggg tggggctgca gccagaaggg tcgcaaaagc gccgaggcca acccacgcag
     6841 cccacaaatg cacgcttggt aaccgaggta ccaggaggat ggcaaatatg actaaaaaaa
     6901 tttcattcat tattaacggc caggttgaaa tctttcccga aagtgatgat ttagtgcaat
     6961 ccattaattt tggtgataat agtgtttacc tgccaatatt gaatgactct catgtaaaaa
     7021 acattattga ttgtaatgga aataacgaat tacggttgca taacattgtc aattttctct
     7081 atacggtagg gcaaagatgg aaaaatgaag aatactcaag acgcaggaca tacattcgtg
     7141 acttaaaaaa atatatggga tattcagaag aaatggctaa gctagaggcc aattggatat
     7201 ctatgatttt atgttctaaa ggcggccttt atgatgttgt agaaaatgaa cttggttctc
     7261 gccatatcat ggatgaatgg ctacctcagg atgaaagtta tgttcgggct tttccgaaag
     7321 gtaaatctgt acatctgttg gcaggtaatg ttccattatc tgggatcatg tctatattac
     7381 gcgcaatttt aactaagaat cagtgtatta taaaaacatc gtcaaccgat ccttttaccg
     7441 ctaatgcatt agcgttaagt tttattgatg tagaccctaa tcatccgata acgcgctctt
     7501 tatctgttat atattggccc caccaaggtg atacatcact cgcaaaagaa attatgcgac
     7561 atgcggatgt tattgtcgct tggggagggc cagatgcgat taattgggcg gtagagcatg
     7621 cgccatctta tgctgatgtg attaaatttg gttctaaaaa gagtctttgc attatcgata
     7681 atcctgttga tttgacgtcc gcagcgacag gtgcggctca tgatgtttgt ttttacgatc
     7741 agcgagcttg tttttctgcc caaaacatat attacatggg aaatcattat gaggaattta
     7801 agttagcgtt gatagaaaaa cttaatctat atgcgcatat attaccgaat gccaaaaaag
     7861 attttgatga aaaggcggcc tattctttag ttcaaaaaga aagcttgttt gctggattaa
     7921 aagtagaggt ggatattcat caacgttgga tgattattga gtcaaatgca ggtgtggaat
     7981 ttaatcaacc acttggcaga tgtgtgtacc ttcatcacgt cgataatatt gagcaaatat
     8041 tgccttatgt tcaaaaaaat aagacgcaaa ccatatctat ttttccttgg gagtcatcat
     8101 ttaaatatcg agatgcgtta gcattaaaag gtgcggaaag gattgtagaa gcaggaatga
     8161 ataacatatt tcgagttggt ggatctcatg acggaatgag accgttgcaa cgattagtga
     8221 catatatttc tcatgaaagg ccatctaact atacggctaa ggatgttgcg gttgaaatag
     8281 aacagactcg attcctggaa gaagataagt tccttgtatt tgtcccataa taggtaaaag
     8341 tatggaaaat gaatcaaaat ataaaaccat cgaccacgtt atttgtgttg aaggaaataa
     8401 aaaaattcat gtttgggaaa cgctgccaga agaaaacagc ccaaagagaa agaatgccat
     8461 tattattgcg tctggttttg cccgcaggat ggatcatttt gctggtctgg cggaatattt
     8521 atcgcggaat ggatttcatg tgatccgcta tgattcgctt caccacgttg gattgagttc
     8581 agggacaatt gatgaattta caatgtctat aggaaagcag agcttgttag cagtggttga
     8641 ttggttaact acacgaaaaa taaataactt cggtatgttg gcttcaagct tatctgcgcg
     8701 gatagcttat gcaagcctat ctgaaatcaa tgcttcgttt ttaatcaccg cagtcggtgt
     8761 tgttaactta agatattctc ttgaaagagc tttagggttt gattatctca gtctacccat
     8821 taatgaattg ccggataatc tagattttga aggccataaa ttgggtgctg aagtctttgc
     8881 gagagattgt cttgattttg gttgggaaga tttagcttct acaattaata acatgatgta
     8941 tcttgatata ccgtttattg cttttactgc aaataacgat aattgggtca agcaagatga
     9001 agttatcaca ttgttatcaa atattcgtag taatcgatgc aagatatatt ctttgttagg
     9061 aagttcgcat gacttgagtg aaaatttagt ggtcctgcgc aatttttatc aatcggttac
     9121 gaaagccgct atcgcgatgg ataatgatca tctggatatt gatgttgata ttactgaacc
     9181 gtcatttgaa catttaacta ttgcgacagt caatgaacgc cgaatgagaa ttgagattga
     9241 aaatcaagca atttctctgt cttaaaggat cctgaggagg aaaaacaggt atgacttcat
     9301 atgttgataa acaagaaatt acagcaagct cagaaattga tgatttgatt ttttcgagcg
     9361 atccattagt gtggtcttac gacgagcagg aaaaaatcag aaagaaactt gtgcttgatg
     9421 catttcgtaa tcattataaa cattgtcgag aatatcgtca ctactgtcag gcacacaaag
     9481 tagatgacaa tattacggaa attgatgaca tacctgtatt cccaacatcg gtttttaagt
     9541 ttactcgctt attaacttct caggaaaacg agattgaaag ttggtttacc agtagcggca
     9601 cgaatggttt aaaaagtcag gtggcgcgtg acagattaag tattgagaga ctcttaggct
     9661 ctgtgagtta tggcatgaaa tatgttggta gttggtttga tcatcaaata gaattagtca
     9721 atttgggacc agatagattt aatgctcata atatttggtt taaatatgtt atgagtttgg
     9781 tggaattgtt atatcctacg acatttaccg taacagaaga acgaatagat tttgttaaaa
     9841 cattgaatag tcttgaacga ataaaaaatc aagggaaaga tctttgtctt attggttcgc
     9901 catactttat ttatttactc tgccattata tgaaagataa aaaaatctca ttttctggag
     9961 ataaaagcct ttatatcata accggaggcg gctggaaaag ttacgaaaaa gaatctctga
    10021 aacgtgatga tttcaatcat cttttatttg atactttcaa tctcagtgat attagtcaga
    10081 tccgagatat atttaatcaa gttgaactca acacttgttt ctttgaggat gaaatgcagc
    10141 gtaaacatgt tccgccgtgg gtatatgcgc gagcgcttga tcctgaaacg ttgaaacctg
    10201 tacctgatgg aacgccgggg ttgatgagtt atatggatgc gtcagcaacc agttatccag
    10261 catttattgt taccgatgat gtcgggataa ttagcagaga atatggtaag tatcccggcg
    10321 tgctcgttga aattttacgt cgcgtcaata cgaggacgca gaaagggtgt gctttaagct
    10381 taaccgaagc gtttgatagt tgatatcctg caatcaagct tatcgatgtc gacgtagtta
    10441 actagcgtac gatcgactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
    10501 gcctttcgtt ttatctgttg tttgtccggc catcatggcc gcggtgatca
//