质粒名称: | pRK172-6xHis-0N4R-P301S-tau-HiBiT |
---|---|
出品公司: | Addgene |
目录编号: | 187835 |
存储实验室: | William McEwan |
载体骨架基本信息 |
|
载体名称: | pRK172 |
载体出品公司: | Clontech |
空载体大小: | 2542bp |
完整质粒大小: | 3760bp |
载体类型: | 哺乳细胞表达 |
载体抗性: | 氨苄霉素 |
生长温度: | 37 ℃ |
宿主菌: | DH5a |
拷贝数: | 未知 |
插入基因信息 |
|
插入基因名称: | 6XHis-0N4R-P301S-tau-HiBiT、Tau-HiBiT |
物种来源: | H. sapiens (human) |
插入大小: | 1218bp |
启动子: | T7 |
标签/融合蛋白: |
6xHistidine (N terminal on insert)
HiBiT (C terminal on insert)
|
克隆方法: | 限制性内切酶 |
5’克隆位点: | NdeI (not destroyed) |
3’克隆位点: | EcoRI (not destroyed) |
5’测序引物 | GATCTCGATCCCGCGAAATT |
3’测序引物 | CAAGACCCGTTTAGAGGCCC |
文献引用方法 |
|
材料和方法部分: | pRK172-6xHis-0N4R-P301S-tau-HiBiT was a gift from William McEwan (Addgene plasmid # 187835 ; http://n2t.net/addgene:187835 ; RRID:Addgene_187835) |
参考文献部分: | Cholesterol determines the cytosolic entry and seeded aggregation of tau. Tuck BJ, Miller LVC, Katsinelos T, Smith AE, Wilson EL, Keeling S, Cheng S, Vaysburd MJ, Knox C, Tredgett L, Metzakopian E, James LC, McEwan WA. Cell Rep. 2022 May 3;39(5):110776. doi: 10.1016/j.celrep.2022.110776. 10.1016/j.celrep.2022.110776 PubMed 35508140 |
买家导航
pRK172-6xHis-0N4R-P301S-tau-HiBiT载体质粒基本信息
pRK172-6xHis-0N4R-P301S-tau-HiBiT质粒图谱载体图谱和pRK172-6xHis-0N4R-P301S-tau-HiBiT载体序列质粒序列多克隆位点信息
pRK172-6xHis-0N4R-P301S-tau-HiBiT质粒载体简介
pRK172-6xHis-0N4R-P301S-tau-HiBiT质粒序列载体序列
LOCUS pRK172-6xHis-0N4R-P301S-tau-HiBiT pRK172-6xHis-0N4R-P301S-tau-HiBiT (Plasmid #187835) Print 3766 bp DNA circular SYN 25-AUG-2024 DEFINITION Expression of 6xHis-tagged P301S tau of 0N4R isoform with a C-terminal HiBiT tag for bimolecular luminescence with LgBiT.. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3766) AUTHORS Tuck BJ, Miller LVC, Katsinelos T, Smith AE, Wilson EL, Keeling S, Cheng S, Vaysburd MJ, Knox C, Tredgett L, Metzakopian E, James LC, McEwan WA TITLE Cholesterol determines the cytosolic entry and seeded aggregation of tau. JOURNAL Cell Rep. 2022 May 3;39(5):110776. doi: 10.1016/j.celrep.2022.110776. PUBMED 35508140 REFERENCE 2 (bases 1 to 3766) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3 https://www.biofeng.com COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep."; date: "2022-05-3"; volume: "39(5):110776. doi"; pages: " 10.1016/j.celrep.2022.110776" FEATURES Location/Qualifiers source 1..3766 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind complement(19..37) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 105..209 /gene="bla" /label=AmpR promoter CDS 210..1070 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(428..447) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1241..1829 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1730..1749 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 1983..2000 /label=L4440 /note="L4440 vector, forward primer" misc_feature 2015..2154 /label=bom /note="basis of mobility region from pBR322" primer_bind complement(2080..2099) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 2250..2272 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 2325..2344 /label=T7 /note="T7 promoter, forward primer" promoter 2325..2343 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" RBS 2375..2397 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 2411..2428 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" primer_bind complement(3679..3697) /label=T7 Term /note="T7 terminator, reverse primer" terminator 3693..3740 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" ORIGIN 1 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa 61 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt 121 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa 181 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta 241 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag 301 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca 361 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta 421 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc 481 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc 541 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca 601 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc 661 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca 721 taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac 781 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg 841 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg 901 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg 961 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac 1021 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc 1081 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct 1141 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc 1201 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 1261 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 1321 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 1381 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 1441 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 1501 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 1561 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 1621 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 1681 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 1741 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 1801 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 1861 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg 1921 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc 1981 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc 2041 atctgtgcgg tatttcacac cgcaatggtg cactctcagt acaatctgct ctgatgccgc 2101 atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2161 cacccgccaa cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc 2221 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc 2281 gtcatcaccg aaacgcgcga ggcaggatct cgatcccgcg aaattaatac gactcactat 2341 agggagacca caacggtttc cctctagaaa taattttgtt taactttaag aaggagatat 2401 acatatgatg catcaccatc accatcacgc tgagccccgc caggagttcg aagtgatgga 2461 agatcacgct gggacgtacg ggttggggga caggaaagat caggggggct acaccatgca 2521 ccaagaccaa gagggtgaca cggacgctgg cctgaaagct gaagaagcag gcattggaga 2581 cacccccagc ctggaagacg aagctgctgg tcacgtgacc caagctcgca tggtcagtaa 2641 aagcaaagac gggactggaa gcgatgacaa aaaagccaag ggggctgatg gtaaaacgaa 2701 gatcgccaca ccgcggggag cagcccctcc aggccagaag ggccaggcca acgccaccag 2761 gattccagca aaaaccccgc ccgctccaaa gacaccaccc agctctggtg aacctccaaa 2821 atcaggggat cgcagcggct acagcagccc cggctcccca ggcactcccg gcagccgctc 2881 ccgcaccccg tcccttccaa ccccacccac ccgggagccc aagaaggtgg cagtggtccg 2941 tactccaccc aagtcgccgt cttccgccaa gagccgcctg cagacagccc ccgtgcccat 3001 gccagacctg aagaatgtca agtccaagat cggctccact gagaacctga agcaccagcc 3061 gggaggcggg aaggtgcaga taattaataa gaagctggat cttagcaacg tccagtccaa 3121 gtgtggctca aaggataata tcaaacacgt cagtggaggc ggcagtgtgc aaatagtcta 3181 caaaccagtt gacctgagca aggtgacctc caagtgtggc tcattaggca acatccatca 3241 taaaccagga ggtggccagg tggaagtaaa atctgagaag cttgacttca aggacagagt 3301 ccagtcgaag attgggtccc tggacaatat cacccacgtc cctggcggag gaaataaaaa 3361 gattgaaacc cacaagctga ccttccgcga gaacgccaaa gccaagacag accacggggc 3421 ggagatcgtg tacaagtcgc cagtggtgtc tggggacacg tctccacggc atctcagcaa 3481 tgtctcctcc accggcagca tcgacatggt agactcgccc cagctcgcca cgctagctga 3541 cgaggtgtct gcctccctgg ccaagcaggg tttgggttca ggttcagtga gcggctggcg 3601 cctgttcaag aagatcagct aggaattccg atccggctgc taacaaagcc cgaaaggaag 3661 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac 3721 gggtcttgag gggttttttg ctgaaaggag gaactatatc cggata //
pRK172-6xHis-0N4R-P301S-tau-HiBiT载体图谱质粒图谱pdf版和pRK172-6xHis-0N4R-P301S-tau-HiBiT载体序列质粒序列等相关资料下载
pRK172-6xHis-0N4R-P301S-tau-HiBiT质粒载体应用举例
- 上一篇:pCMV/SB10
- 下一篇:没有了