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LOCUS pCerulean-Venus-MCS 5431 bp DNA circular SYN 12-JUL-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5431)
AUTHORS Biofeng
TITLE Direct Submission
JOURNAL Exported Jul 12, 2023 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5431
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 6..719
/codon_start=1
/product="Venus YFP with monomerizing A206K mutation (Nagai
et al., 2002; Kremers et al., 2006)"
/label=mVenus
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
polyA_signal 880..1001
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(1008..1463)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1490..1594
/gene="bla"
/label=AmpR promoter
promoter 1596..1953
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 1804..1939
/label=SV40 ori
/note="SV40 origin of replication"
CDS 1988..2782
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3014..3061
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 3390..3978
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
enhancer 4153..4456
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 4457..4660
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
regulatory 4699..4708
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 4705..5421
/codon_start=1
/product="enhanced monomeric variant of CFP (Rizzo et al.,
2004)"
/label=mCerulean
/note=" mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
ORIGIN
1 gatctgtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
61 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
121 acggcaagct gaccctgaag ctcatctgca ccaccggcaa gctgcccgtg ccctggccca
181 ccctcgtgac caccctcggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga
241 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
301 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
361 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
421 acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac aagcagaaga
481 acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc gtgcagctcg
541 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
601 actacctgag ctaccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg
661 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg
721 aattctgcag tcgacggtac cgcgggcccg ggatccaccg gatctagata actgatcata
781 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
841 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
901 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
961 cattctagtt gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt
1021 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
1081 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
1141 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
1201 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
1261 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
1321 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
1381 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
1441 taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
1501 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
1561 ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg
1621 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
1681 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
1741 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
1801 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
1861 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
1921 ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc
1981 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
2041 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
2101 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
2161 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
2221 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
2281 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
2341 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
2401 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
2461 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat
2521 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
2581 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
2641 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
2701 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
2761 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
2821 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
2881 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
2941 ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat accggaagga
3001 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt
3061 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat
3121 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa
3181 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca
3241 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
3301 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
3361 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
3421 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
3481 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
3541 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
3601 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
3661 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
3721 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
3781 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
3841 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
3901 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
3961 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
4021 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
4081 accgccatgc attagttatt aatagtaatc aattacgggg tcattagttc atagcccata
4141 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
4201 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
4261 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
4321 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
4381 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
4441 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
4501 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
4561 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
4621 cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat
4681 ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg
4741 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
4801 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
4861 aagctgcccg tgccctggcc caccctcgtg accaccctga cctggggcgt gcagtgcttc
4921 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
4981 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
5041 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
5101 gaggacggca acatcctggg gcacaagctg gagtacaacg ccatcagcga caacgtctat
5161 atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc
5221 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
5281 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc
5341 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
5401 ggcatggacg agctgtacaa gtccggactc a
//
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