质粒名称: | pVS1-VIR2 |
---|---|
出品公司: | Addgene |
目录编号: | 134745 |
存储实验室: | Qi-Jun Chen |
载体骨架基本信息 |
|
载体名称: | pVS1 |
完整质粒大小: | 39291 bp |
载体类型: | Vir gene expression |
筛选标记: | NEB Stable |
载体抗性: | Spectinomycin |
生长温度: | 37 ℃ |
宿主菌: | DH5a |
拷贝数: | 高拷贝 |
插入基因信息 |
|
插入基因名称: | vir gene cluster |
物种来源: | 未知 |
克隆方法: | 未知 |
5’克隆位点: | Unknown (unknown if destroyed) |
文献引用方法 |
|
材料和方法部分: | pVS1-VIR2 was a gift from Qi-Jun Chen (Addgene plasmid # 134745 ; http://n2t.net/addgene:134745 ; RRID:Addgene_134745) |
参考文献部分: | A Novel Ternary Vector System United with Morphogenic Genes Enhances CRISPR/Cas Delivery in Maize. Zhang Q, Zhang Y, Lu MH, Chai YP, Jiang YY, Zhou Y, Wang XC, Chen QJ. Plant Physiol. 2019 Sep 26. pii: pp.19.00767. doi: 10.1104/pp.19.00767. 10.1104/pp.19.00767 PubMed 31558579 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pVS1-VIR2 39291 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Virulence and replication helper. ACCESSION . VERSION . KEYWORDS pVS1-VIR2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 39291) AUTHORS Zhang Q, Zhang Y, Lu MH, Chai YP, Jiang YY, Zhou Y, Wang XC, Chen QJ TITLE A Novel Ternary Vector System United with Morphogenic Genes Enhances CRISPR/Cas Delivery in Maize. JOURNAL Plant Physiol. 2019 Sep 26. pii: pp.19.00767. doi: 10.1104/pp.19.00767. PUBMED 31558579 REFERENCE 2 (bases 1 to 39291) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.biofeng.com FEATURES Location/Qualifiers source 1..39291 /organism="synthetic DNA construct" /mol_type="other DNA" RBS 3221..3229 /label=Shine-Dalgarno sequence /note="full consensus sequence for ribosome-binding sites upstream of start codons in E. coli; complementary to a region in the 3' end of the 16S rRNA (Chen et al., 1994)" CDS 16098..16109 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" CDS complement(30147..31295) /codon_start=1 /product="trans-acting replication protein that binds to and activates oriV" /label=trfA /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR" CDS 34347..34976 /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 StaA /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" CDS 35405..36478 /codon_start=1 /product="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 RepA /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI LVMRYRNLIEGEASAGS" rep_origin 36544..36738 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" primer_bind complement(36974..36996) /label=pGEX 3' /note="pGEX vectors, reverse primer" misc_feature 37082..37222 /label=bom /note="basis of mobility region from pBR322" primer_bind 37137..37156 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(37237..37254) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(37408..37996) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(37488..37507) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(38252..39043) /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" ORIGIN 1 aacaggctta tgtccactgg gttcgaattc attaccctgt tatccctaat gcagagccac 61 cccatcagga atatcgccca ttccgtcatc agcgtggtga cgtgcatcag gcgcgcttgt 121 tgcagaactt taagtgcttc tttcgattcg agatctcagc aaaagttctc cgcaaacctt 181 caccgcgggc ttacgtgtct tttgtaacaa attgcgacgc agttgatatc cacttgaaac 241 attagtccga aattatcgag atctccgctg ataaggtatc gaaatggcga taaaattggt 301 attgatactc gtattcacac tctttcccgc ggcagacgct gcatatgcga atgaccgcgc 361 caacggtttc atgtggtcaa acgggggcga aactggagtg aggcttcctc ttcgggtgtt 421 caatgccaag ccagccaaga acacggtggc gatcatttat tccggagacg ctggatggca 481 aaatatcgat gaggcgattg gtacctatct gcagacggaa gggattcctg tcattggcgt 541 cagttcactt cggtatttct ggtcggagcg gtccccaagc gaaactgcta aggatcttgg 601 tcacataatc gatgtctaca ccaagcattt cggtgtgcag aatgttttac ttgtaggata 661 ttctttcggc gcggacgtca tgccggcaag cttcaatagg cttacgcttg agcaaaaaaa 721 tcgggttaag caaatctctc tcttggcatt gtcacatcaa gtcgactatg tcgtctcatt 781 taggggctgg ctccaactcg aaacggaagg taagggcggc aatcctctgg atgatctcag 841 atccattgac cctgcaatcg tccaatgcat gtacgggcgc gaagaccgta ataatgcttg 901 cccatctctc cgacagaccg gcgcagaggt gataggcttc agcggaggcc atcactttga 961 taatgatttc aaaaaactgt ctacgcgcgt cgtctcaggc ctcgtggcac gcctaagtca 1021 tcagtaatct ttagttcctg caccgcttta gtattgactg ggatagcgac gcctgtgatg 1081 cagacatcgg atattgtgtc gttaagtaaa aggccttcgt ctgatcgcga gactcgctag 1141 tggttttcag gtgagtgaga tgttttgccg caagttgcgc tgagatcgca tctgcctgcg 1201 gctgccgcac ctccagattg gcagcaacaa gatcatcctt caagggaaga tgcctataac 1261 gcatgtgttg acgactttcg cctcgtgaat gatccggtct gttcccgaca gtgggatgcc 1321 gtcgcgatag agcaggctct cgcgccagga ggaatttctc cgatatgact tctctccttc 1381 tacgtgccca aagcagcaac gaaatcatct atcgcggcca aaagagcggt catgtgaaag 1441 cacccttgcc agtcaattcc ctggccaagg tcagcacggc gatactgcga ggtgttgcta 1501 caccacggag gcgatctaga aggacgattt catcatttag gccgcacact cataaatcag 1561 gtttgcaaat cggtctgatt ttgattcatt gaggcttgac ttggaggcca ctgataaccc 1621 gtccccgctt tgatgtcacc gatttcgaat ggactgttgt tcagtccttt gttcccaaat 1681 aagccgcgtc gcgtgccgcg cgttgacgcg ggtgatcaat gtcatcttgt ggcgcttccg 1741 gacgggcccg ccctgggcag acgttcctga tcgatacggt tcctgtacaa cctgctacaa 1801 tcgctttgta cgatggcgtg aggcagacgt ctgggatcat attctgagtg tgatttccaa 1861 ggctttcgat agaatgtcgt catgattgac agttcctgtg tccgcgtcca ccaacatgcg 1921 gcgacgggaa acagcgggat caagacgatg gctgtatggg acgttcccgt ggcggtttga 1981 ccaccaagat tcacgctgtt gtcgatgccg acggttgacc gatccgtctt gcgctgacag 2041 cgggtcaagc ccatgaccgc cgcatgaccg aaacattttt acaaataatc gcttagagtg 2101 cgtttctgct ggcggacaag gcatatgata ccaacgcgat aagagcattt gcaaagcgca 2161 agcaggcatg ggccaatatt cctgccaaga gcaatcggaa ggaagcttcc cgttcatcga 2221 atgggtttac agacagcgta acctcgccga gcattttcgt tctgagcctg acctgaccaa 2281 ctgcggcctg aataggtcga tccggttgct tagtcatgga tgcgcggttc tcggtccatg 2341 ttgcgttcca agacgccggg cgaggtttct cgcttcaatt gaaatcataa agaagcaatt 2401 gaaaattttc gagtaaccga ccctcccgat aatcttcaac ataaaacaac gcacttcttc 2461 caacgggaga ggcggtgtta gttgcgagct aaggagataa ggtatgctta agagatcggg 2521 gtcgctttct cttgccttga tggtgagctt ctgttcgtcg agccttgcca cgccactctc 2581 atctgctgag tttgaccatg ttgctcgcaa gtgtgcccca tcagttgcga catctacgct 2641 tgcggcgata gctaaggtgg agagtcgctt tgatccttta gcgattcatg acaacacgac 2701 cggcgaaacg cttcactggc aagatcacag ccaagcaacc caagtcgtca ggcgccgtct 2761 cgatgcacgg cattcgctgg atgttggcct catgcaaata aactctcgaa atttttctat 2821 gctcggtctg acacctgacg gtgcgctcca ggcgtgcaca tcattatctg ccgctgcaaa 2881 catgctgaaa agtcgttatg caggcggcga aacgattgac gagaagcaat ttgcgcttcg 2941 tcgggcgatc tccgcttaca acaccggtaa tttcatcggc ggttttgcaa acggctacgt 3001 gcgaaaagtt gaaacagctg ctcaatcgct ggtgcccgcg ttaatcgagc ctccaaaaga 3061 cgatcacgag gcgctaaaat ccgaagagac gtgggatgtt tgggggtcat atcagcgccg 3121 ctcgcaggag gatggcgctg gcggtttaat cgctccgcca ccgccacacc aggacaacgg 3181 caaatccgca gacgacaatc aagtcttatt cgacttatac taaggaggtg cgcattgatg 3241 cgatgctttg agagataccg tttacatcta aatcgcctct cgctctcgaa tgcgatgatg 3301 cgcgtgatat cgagctgcgc cccaagcttg tgcggtgcaa ttgcatggag catttcctca 3361 tccggacccg ccgcagcgca atctgcgggt ggcggcactg accccgccac aatggttaac 3421 aatatatgca cgtttatcct tggtccgttc ggccagtcac tcgctgttct cggcattgtc 3481 gctatcggga tctcctggat gttcgggcgg gcttcgcttg ggctggttgc cggcgtcgtc 3541 ggcggcattg ttatcatgtt tggggcgagc ttcctcggcc aaacgctcac tggcggtagt 3601 tgatggctga tcgtttggaa gaatcgaccc tttacctcgc agccacacgg cccgcattgt 3661 ttcttggggt gccactgaca ttggcagggt tattcatgat gttcgccggc tttgtcatcg 3721 ttatcgttca gaacccgctc tacgaagtcg ttctcgtgcc gttatggttt gcagcccggc 3781 tcatcgtgga gcgagactac aatgcggcga gcgtcgtcct gctatttttg cggaccgcgg 3841 gaagaagcat tgatagtgca gtttgggggg gcgctactgt tagcccaaat ccaattaggg 3901 ttcccccacg agggagagga atggtgtgat gctcggcgcg agtggaacga ccgaaagatc 3961 cggtgagatc tatctccctt atattggcca cctcagcgac catatcgtcc ttcttgaaga 4021 cggatcgatc atgaccattg cgagaattga tggcgttgca ttcgagcttg aggaaactga 4081 aatgcgcaat gcgcgttgtc gtgcgttcaa cacgctgttg cgcaatatcg ctgatgatca 4141 tgtgtcaata tatgctcacc tcgtacgtca tgccgacgtg ccatcatcgg cgccgcgaca 4201 cttccgtagt gttttcgccg ctagcctgaa cgaagctttt gaacagcgcg tgctctccgg 4261 ccaactcctc cgcaatgaac acttccttac gttgattgtc tacccacagg cggctttagg 4321 gaaggtaaag aggaggttca ccaagctaag cggaaaaagg gaaaacgatc tcacgggcca 4381 gatcaggaac atggaagatc tttggcatgt tgtcgctggc tctcttaaag cgtatggcct 4441 gcatcgtctt ggcatccgcg agaagcaggg tgtgctcttc accgaaattg gcgaagcgct 4501 acggttgatc atgactggtc ggttcacacc ggttccggtc gtcagcggct cactcggcgc 4561 ttcgatttat accgacagag tcatttgcgg caagcgagga ctcgagatca gaacgccaaa 4621 agacagttac gttggatcca tctattcgtt tcgcgaatac cctgcaaaaa cacggccggg 4681 catgctcaac gcgctgctat ccctcgattt tccacttgtt ctcacgcaga gtttttcgtt 4741 cctgactcgc ccgcaagcgc acgcgaaact tagcctcaaa tcgagccaga tgctgagttc 4801 cggcgataaa gccgtgactc aaatcggcaa attatccgag gctgaggacg cacttgcgag 4861 caacgaattc gttatgggct cacatcattt gagcctttgc gtctatgcag acgatctcaa 4921 tagtcttggg gacaggggcg cgcgggctcg gacacgaatg gcggatgctg gagccgtggt 4981 tgtccaagaa ggtattggta tggaagcggc ctattggtcc caattgccgg ggaattttaa 5041 gtggcgcaca cgccctggcg caatcacttc acgcaatttc gcagggtttg tctctttcga 5101 aaactttcca gagggcgcca gctcaggcca ctggggcaac gcgattgccc gatttcgtac 5161 caatggcgga acgcctttcg actatatccc gcatgagcac gatgttggca tgacggcaat 5221 attcgggcct atcgggaggg gtaagacgac gctcatgatg tttgttctag ccatgctcga 5281 acagagcatg gtcgaccgtg caggtacggt cgtgttcttt gacaaggacc ggggtggcga 5341 attgctggtt cgcgccacag gaggaacata tttggcactt cacagaggca cacccagcgg 5401 gttggcgccg ttgcgtggcc tagaaaacac agcagcctca cacgattttc tgcgcgaatg 5461 gatcgtggct ctcatcgaga gtgatggtcg gggtgggatt tctccggaag agaaccgccg 5521 tctggtccgt ggtatccatc gtcagctctc gtttgatcca caaatgcgtt caatcgcggg 5581 gttacgtgaa tttttgttgc atgggcccgc cgaaggcgca ggagcgcggc tccaacgctg 5641 gtgccggggc catgcgcttg gctgggcatt tgacggcgaa gttgacgaag taaagttaga 5701 tccgtcgatt accggcttcg acatgacgca tcttctcgaa tacgaggaag tatgcgctcc 5761 cgctgcagca tatctcctgc atcggattgg agccatgatc gacggccgcc gttttgtgat 5821 gagctgcgat gagtttcgcg cctatttgtt aaaccctaaa ttttcgactg tcgtcgacaa 5881 attcctcctg accgttcgaa aaaacaacgg gatgctaata ctggcaacgc agcaaccaga 5941 gcatgttctg gaatcgccgc taggagccag cttggttgcg caatgtatga cgaagatttt 6001 ctatccatca ccaaccgcag atcgatcggc ttatgtcgat ggactgaaat gtaccgaaaa 6061 ggaatttcag gcgatccgtg aagacatgac ggtcggcagc cgtaagtttc ttcttaaacg 6121 agaaagtgga agcgtcatct gcgaatttga tctgcgggat atgcgtgaat atgtcgccgt 6181 gctttcgggg cgtgccaaca cggtgcgctt tgcaactcga ctacgcgagg cacaagaagg 6241 caactcatct ggctggctca gcgaattcat ggcccgtcac cacgaggcag aagattgata 6301 aggtaggaaa cgatgaagac gacgcaactt attgcaacag ttttgacctg cagctttcta 6361 tatattcagc ccgcgcgggc gcagtttgtt gttagcgacc cggcaacgga ggctgagacg 6421 ctcgcgactg cgctcgcgac tgcggagaat ctcactcaga ctatagcgat ggttacgatg 6481 ttgacgtcgg cctacggcgt tactggacta ctgacttcgc tcaaccagaa aaatcagtat 6541 ccttcgacga aggacctaga caatgaaatg ttttcgccgc gaatgccaat gtcgaccacg 6601 gcacgtgcga tcaccagcga tacagatcgt gcagtcgtgg gtagtgatgc tgaagcggac 6661 ctgttgcgat cgcagatcac cggttccgca aacagcgctg gcattgcggc tgacaatctg 6721 gaaacgatgg acaaacgctt gacggcgaat gctgatacgt ctgctcagct ttcccgatct 6781 cgcaatatca tgcaggcaac cgtgaccaat ggtttgcttc tcaagcagat ccatgacgca 6841 atgattcaaa atgtacaggc gacaagccta ttaacgatga ctaccgcgca ggccggcctt 6901 cacgaggcgg aagaggcggc cgctcaacgc aaggagcatc aaaagaccgc tgtcatcttt 6961 ggtgccctcc cctaaggctg ggcgatttgt tcatccgccc gcatcctcgc cgaatgcgag 7021 ctcattttat ccaacattat gcgacaaacc agtcaagttc aggtccaatc gatgaatttc 7081 acgattccgg cgccgtttac ggccattcat acgatcttcg atgtagcctt cacgacaggc 7141 ttggactcga tgcttgagac tatccaggag gcggtgagtg cgccattgat cgcctgtgtc 7201 actctttgga ttattgttca gggtatttta gtcatacgcg gcgaagtcga tacccgtagc 7261 ggtatcactc gggtgatcac ggtcaccatc gttgttgctc taattgttgg gcaggctaac 7321 taccaagact atgtggtttc catcttcgaa aagacggtcc caaactttgt tcagcagttt 7381 agtgtaaccg gcttgcctct gcagactgtt ccggcacagt tggatacaat gttcgccgtg 7441 acccaggccg tttttcagaa aatcgcatcc gaaatcggtc cgatgaacga ccaggacatc 7501 cttgctttcc aaggggcaca gtgggtcctt tacggcacgc tctggtctgc cttcggagtt 7561 tacgacgccg ttggaattct cacgaaagtg cttctcgcga tcgggcctct gatcctcgtc 7621 ggatatattt ttgatcgcac gcgggacatc gcagctaagt ggatcgggca acttatcacc 7681 tacggtctct tgcttctcct cttaaacctc gtggcaacga tcgtcatcct aaccgaagcg 7741 actgcgctca cccttatgct tggtgtaatc accttcgccg gtacgaccgc ggccaagatc 7801 attggtcttt acgaactcga tatgtttttt ctgacagggg atgcgctcat tgtcgctttg 7861 ccggcgatcg ccggcaacat tggaggcagt tactggagcg gcgcaaccca atctgccagc 7921 agcttgtacc gtcgcttcgc tcaggttgag cgaggctagg tcgcgcaaaa attcgcctca 7981 atggagaatt ctatgaagta ttgcctgctg tgcctagttg tcgctttgag cggctgccag 8041 acaaacgaca cattagcgag ctgcaaaggc ccgatcttcc cgctgaatgt ggggcgatgg 8101 cagcctactc cgtcagatct tcagctcggc aattcgggtg gacgctatga cggggcctga 8161 atatgccatg ctagtggcgc gcgaaagcct tgccgagcac tataaggaag tagaagcctt 8221 tcaaaccgcg cgagcgaaat cggcgcgacg tctctccaaa ctcattgcag ctgtcgcagc 8281 tatcgcgatt ttgggaaatg ttgctcaagc gttcgctata gccacaatgg tgccgttgag 8341 caggcttgtg cccgtatatc tatggatacg gccggacggc accgttgaca gcgaggtgtc 8401 tgtctcgcga ttgcctgcaa ctcaagagga ggccgtcgtt aacgcctcat tgtgggagta 8461 cgttcgcctg cgcgagagtt atgatgccga caccgctcag tacgcctacg acctggtatc 8521 gaacttcagt gccccaacag tgcgccagga ttaccagcaa ttcttcaact atcccaatcc 8581 cagttcgcct caagtcattc ttggcaaacg cggcagggtg gaggtcgagc acatcgcttc 8641 aaatgatgta actccaagca cgcagcaaat tcgctataaa aggaccctcg tcgttgacgg 8701 caaaatgcct gtggtgagta cgtggaccgc gacagttcgc tacgaaaagg tgaccagctt 8761 gcccggcaga ttgagactaa ccaacccggc aggtctggtt gtcacctcct atcagacatc 8821 ggaagatacc gtttcaaacg taggccacag cgaaccatga tcagaaaagc acttttcatt 8881 ttagcatgtt tatttgccgc tgcgactggt gcggaggctg aagacactcc aatggcgggc 8941 aagctagatc cgcgcatgcg ttatttggct tacaatcccg atcaagtggt gcgcctctcg 9001 acggcggttg gagctacttt ggtcgtaaca ttcgccacga acgaaacggt gacagcggtt 9061 gccgtttcaa atagcaaaga tctagcagcc ctaccgcggg gaaattatct atttttcaag 9121 gcaagccagg tcctcacgcc tcagccagta atcgtgctaa ccgcaagcga ctccgggatg 9181 cgccgttatg ttttcagtat aagttccaag actctgtccc acctcgataa agagcagccc 9241 gatctctatt acagcgtcca attcgcctac cccgccgacg atgcggcggc tcggcgaagg 9301 gaggcacaac agaaggctgt tgtggacaga ctacacgcgg aagcacaata tcaacggaaa 9361 gctgagaatt tattggatca gcctgtcaca gcccttggtg cggcggacag taattggcac 9421 tacgtcgccc aaggcgatcg ttcgctgttg ccactcgaag tcttcgacaa tggatttacg 9481 acggtattcc actttccggg caatgtacgc ataccctcca tctacaccat caatcctgat 9541 ggcaaggaag ctgttgccaa ctattcagtt aaagggagcg atgtcgagat ttcttcggtt 9601 tcccgaggtt ggcgtctgag ggatggccac acagtactat gtatctggaa caccgcttac 9661 gatcccgttg gccaaaggcc gcaaacgggc acggtgaggc ccgatgtgaa acgcgtcctg 9721 aagggggcga agggatgaat aacgatagtc agcaagcggc acatgaggtt gatgcatctg 9781 gatccctggt ctccgacaaa catcgccggc gtctttcggg gtctcagaaa ttgatcgtcg 9841 gaggtgtcgt tctcgcgtta tcattaagcc tcatttggct aggtgggcgc caaaagaagg 9901 tgaatgagaa cgcatcgccg tcaactttga tcgcaacaaa caccaagcca tttcatccag 9961 ctccgattga ggtgccgccg gatcctccag cggttcaaga ggctgttcag cctgctgctc 10021 ctctaccgcc gaggggcgaa ccggagcggc atgagccacg gccggaagaa acaccgattt 10081 ttgcatatag cagcggcgat caaggggtca gcaaacgcgc cattcagggc gacacgggcc 10141 gaagacaaga aggcaagcgt gacgacaact ccttgccgaa tggcgaagtg tccggcgaga 10201 acgatttgtc gatacgtatg aaacccaccg agctgcagcc cagcagcgcc acgctcttgc 10261 cgcaccccga ttttatggta acgcaaggga caataattcc gtgcatcttg caaaccgcaa 10321 tcgacacaaa tttggcaggc tatgtaaagt gtgtcttgcc tcaggatatt cgtggaacaa 10381 cgaacaatat cgtgcttctt gatcgtggca ccaccgttgt tggcgaaata cagcgtggct 10441 tgcaacaggg agatgggcgc gtttttgtgt tgtgggatcg cgccgagaca cctgaccatg 10501 cgatgatctc gttaacatcg ccaagcgcgg acgaactcgg tcgctcagga ttgccgggct 10561 cggtcgacag ccacttctgg cagcgtttta gcggagctat gctcttgagt gttgttcaag 10621 gcgccttcca ggcagctagc acctacgccg gcagctcggg tggcgggatg agcttcaaca 10681 gctttcaaaa taacggtgag cagacaactg agacagccct taaggcaacc atcaacatac 10741 cgccaaccct gaagaagaat cagggtgaca ccgtttccat tttcgtagca cgggacctcg 10801 atttctttgg tgtttaccag ctccgcctga ctggcggcgc cacgcggggg aggaaccgcc 10861 gctcttaatg aattcaaatt tccgcttaga gataggatac attgtaaatg gaagtggatc 10921 cgcaactacg ctttcttctg aagccgattt tggaatggct cgatgacccg aagactgaag 10981 aaattgcgat caatcgacct ggagaggcat ttgtgcgcca agccggcatt tttaccaaga 11041 tgcctttgcc cgtctcttat gatgatcttg aagatatcgc tattttagcg ggcgcgctga 11101 gaaagcagga tgtcggacca cgtaaccccc tctgcgccac tgaacttcct ggtggtgaac 11161 gactacaaat ctgtctgccg ccgaccgttc cctcgggcac cgtcagcttg accattcgac 11221 ggccaagctc gcgtgtttct ggtcttaaag aagtctcctc ccgttatgat gcttcgaggt 11281 ggaaccagtg gcagacacga aggaaacgcc aaaatcagga tgatgaagct atccttcagc 11341 attttgacaa cggggatttg gaagcgtttc tgcacgcatg cgtcgtcagc cgactgacga 11401 tgttgctatg tggccctacc ggaagcggca agacaacaat gagcaagacc ttgatcagcg 11461 ccatcccccc ccaggaaagg ctaatcacca tagaagatac gctcgaactc gtcattccac 11521 acgataatca tgttagacta ctctactcca agaacggtgc tgggctgggt gctgtgagcg 11581 ccgagcactt gctccaagca agtctgcgta tgcggccgga ccggatattg cttggcgaga 11641 tgcgcgacga tgcagcatgg gcttatctga gtgaagtcgt ctcgggacat ccgggatcga 11701 tttcaacaat acacggcgcg aatccaatcc aaggattcaa gaaactgttt tcccttgtca 11761 aaagtagcgc ccaaggtgct agcttggaag atcgcacact gattgacatg ctctctacgg 11821 cgatcgatgt catcattcca ttccgtgcct atgaggacgt ttatgaagta ggcgagatct 11881 ggctcgcggc ggacgcacga cgccggggcg agaccatagg cgatctcctt aatcaatagt 11941 agctgtaacc tcgaagcgtt tcacttgtaa caacgattga gaacttttgt cataaaattg 12001 aaatacttgg ttcgcatttt cgtcatccgc ggtcagccgc aattctgacg aactgcccat 12061 ttagctggag atgattgtac atccttcacg tgaaaatttc tcaagcgctg tgaacaaggg 12121 ttcagatttt agattgaaag gtgagccgtt gaaacacgtt cttcttatcg atgacgatgt 12181 cgctatgcgg catcttatta tcgaatacct tacgatccac gccttcaaag tgaccgcggt 12241 agccgacagc acccagttca ctagagtact ctcttccgcg acggtcgatg tcgtggttgt 12301 tgatctaaat ttaggtcgtg aagatgggct tgagatcgtt cgaaatctgg cggcaaagtc 12361 tgatattcca atcataatta tcagtggcga ccgccttgag gagacggata aagttgttgc 12421 actcgagcta ggagcaagtg attttatcgc taagccgttt agtacgagag agtttcttgc 12481 acgcattcgg gttgccttgc gcgtgcgccc caacgttgtc cgctccaaag accgacggtc 12541 tttttgtttt actgactgga cacttaatct caggcaacgt cgcttgatgt ccgaagctgg 12601 cggtgaggtg aaacttacgg ctggagagtt caatcttctc ctcgcgtttt tagagaaacc 12661 ccgcgacgtt ctatcgcgcg agcaacttct cattgccagt cgagtacgcg acgaggaggt 12721 ttacgacagg agtatagatg ttctcatttt gcggctgcgc cgcaaacttg aggcggatcc 12781 gtcaagccct caactgataa aaacagcaag aggtgccggt tatttctttg acgcggacgt 12841 gcaggtttcg cacgggggga cgatggcagc ctgagccaat tgcatttggc tcttaattat 12901 ctggctcaaa aggtgactga ggacgcggcc agcggcctca aacctacact caatatttgg 12961 tgaggggttc cgataggtcc ctcttcacca attgctcgat ggcttctctc cagcaaagaa 13021 tgacgcgagc gcggcggtag ccagcttgtg gccgaaagct cgagcggtct ccaaccccaa 13081 cggatcaaaa tgacttcgag cgacctcgag caacgcaacc gggaacatgc gtgaggtctg 13141 aacgagaacg gatttttctg tagttgaagg gatcggataa cttttcgggg ccacgcgaaa 13201 tgatccatct gccagcatgc tttcgaaatc gtccaacgcg cgccttaaaa tcatttgtag 13261 cgacttcgag ggactgtatt gccgaacgag gttgtcatat gttttcgaca cttgaggcgc 13321 gggcggtcgc gctgaaagaa aaacctggag ctttttcggg gacggaggtg gactaagggc 13381 atccacagtt agcttaagtt gtcgatcggg actgtaaatg tgatcggcga cgagaggctc 13441 acgttgctgg tctttctcgt cggctttttc aggcaagtgc tggaggtcca gcttctgggg 13501 aacaagtgtc gggttgggat ggtggatctc gggtcgagca ccagcaagcc gccgtgcttc 13561 gccgaccgac aatgcgggct tgcgaattgc catcttcaag cctccaagat tttgctgatc 13621 agtttcgaaa tgaccacgac ttcctccatc gcaatccgaa gattcctctc tatgaggcgc 13681 atcgtcggat cagttcccgt gtttagtaat gtaagatgca acatgccgcg ttctttcatc 13741 gcggcaaatg catctctttc atgcatggga gacggtacaa ctggaaggct ctctagcgtc 13801 tctgacatcc tgcgttgcga tgttgtcaat cggccgaccg ggacgcgttg gcgcaaaaca 13861 gctgtaggaa ttgccaaatt ttcactcaac agcagctcga tgacgtagcg gtaggtagat 13921 agtgcctcat cgatgtcgag cggcgttagc atggtgggga tcagaagcag gtttgagcta 13981 gcgatgattg tgttgttgag ctcgctcgag ccgccacgcg tatcggccaa cgcataatca 14041 aatccttcga gctcggcatt ttcataggct gcttcaagaa ggggcatttc gtcggcggaa 14101 tagacttcac agcgaggatc ccaggtactg ctttgtaagg cgttttctct ccatcgcgtc 14161 agaggccggt tttcgtcggc atcaaagagg gccactcgtt taccgtcatt tgccaaagca 14221 gcgcaaaggc ccatgagtgc ggtggttttg ccagcacccc ctttgaaaga acaaaacgtc 14281 aaaagttgca tattctgatc ccgcctatcc tgtgaaaccg gagtgcattt gtatttttgt 14341 tcgtataaat gtttttgtga ttatcgatga gtaaaagcgt tgttacacta tttttatttc 14401 acattcgtta taagacaatt gcaaatgtag caagtatatt cagtattgac tgtaaatgta 14461 ctgttgattt catattgagc agggctagac ttccatccgt ctacccgggc acatttcgtg 14521 ctggagtatc cagaccttcc gctttctttg gaggaagcta tgtcaaaaca caccagagcc 14581 acgtcgagtg agactaccat caaccagcat cgatccctga aagttgaagg gttcaaggtc 14641 gtgagtgccc gtctgcgatc ggccgagtat gaaacctttt cctatcaagc gcgcctgctg 14701 ggactttcgg atagtatggc aattcgcgtt gcggtgcgcc gcatcggggg ctttctcgaa 14761 atagatgcag acacacgaga aaagatggaa gccatacttc agtccatcgg aatactctca 14821 agcaatgtat ccatgcttct atctgcctac gccgaagacc ctcgatcgga tctggaggct 14881 gtgcgagatg aacgtattgc ttttggcgag gctttcgccg ccctcgatgg actactgcgc 14941 tccattttgt ccgtatcccg gcgacggatc gacggtcgct cgttactgaa aggtgccttg 15001 tagcacttga ccacgcacct gacgggagaa aattggatgc ccgatcgcgc tcaagtaatc 15061 attcgcattg tgccaggagg tggaaccaag acccttcagc agataatcaa tcagctggag 15121 tacctgtccc gaaagggaaa gctggaactg cagcgttcag cccggcatct cgatattccc 15181 gttccgccgg atcaaatccg tgagcttgcc caaagctggg ttacggaggc cgggatttat 15241 gacgaaagtc agtcagacga tgacaggcaa caagacttaa caacacacat tattgtaagc 15301 ttccccgcag gtaccgacca aaccgcagct tatgaagcaa gccgggaatg ggcagccgag 15361 atgtttgggt caggatacgg gggtggccgc tataactatc tgacagccta ccacgtcgac 15421 cgcgatcatc cacatttaca tgtcgtggtc aatcgtcggg aacttctggg gcaggggtgg 15481 ctgaaaatat ccaggcgcca tccccagctg aattatgacg gcttacggaa aaagatggca 15541 gagatttcac ttcgtcacgg catagtcctg gatgcgactt cgcgagcaga aaggggaata 15601 gcagagcgac caatcacata tgctgaatat cgacgccttg agcggatgca ggctcaaaag 15661 attcaattcg aagatacaga ttttgatgag acctcgcctg aggaagatcg tcgggacctc 15721 agtcaatcgt tcgatccatt tcgatcggac gcatctgccg gcgaaccgga ccgtgcaacc 15781 cgacatgaca aacaaccgct tgaaccgcac gcccgtttcc aggagcccgc cggctccagc 15841 atcaaagccg acgcacggat ccgcgtacca ttggagagcg agcggggtgc ccaaccatcc 15901 gcgtccaaaa tccctgtaac tgggcatttc gggattgaga cttcgtatgt cgctgaagcc 15961 agcgtgccca aacaaagcgg caattccgat acttctcgcc cggtgactga cgttgccatg 16021 cacacagtcg agcgccagca gcgatcaaaa cgacgtcatg acgaggaggc aggtccgagc 16081 ggagcaaacc gtaaaagatt gaaggccgcg caagttgatt ccgaggcaaa tgtcggtgag 16141 cccgacggtc gcgatgacag caacaaggcg gctgatccgg tgtctgcttc catccgtacc 16201 gagcaaccgg aagcttctcc aacgtgtccg cgtgaccgtc acgatggaga attgggagaa 16261 cgcaaacgtg caagaggtaa tcgtcgcgac gatgggcgcg gggggaccta gagacaggaa 16321 ggaccgaata atggcaaatg gtcagttcac gatacgctct gctcgcccgg cctccgtcgg 16381 actgacaggc gaacggcgtg gagccgcatc cgcctctagc tctgcactgt ccaatgttca 16441 aagagatgtt agggataggc tgattccaac tagctcacca agattaccaa atgcagccat 16501 attgcgtgat tcctcgggaa gagcgtcgac tggtctgcgg tacatggcgg ctactcttca 16561 ttggtctgcg atcgcgccat tatcgctaat aaacagcaac gacctggctc cggccgctta 16621 tgactttgag acgcgaaata acgcaagaaa tgtgactgcc aaagtcggca gggcagtccc 16681 tgttcccaag caaggcgggc tcggcaaaac gctcgcaccc gtacccctta gtacacgtat 16741 atcaagggtc aattccgacc gaagactgcc cgctgacgca gaagaccgcc ctgaaacgcg 16801 cgacccccag aaaggacgtg gcagtcatgg tgcgacgcca accttacatg aaaagattgg 16861 aaccgcgttt gctcgaagat tgcgaaagca tacgtactat attgtttgca gttgctgcca 16921 gacccggagc gcgttgacga tgggtgcaaa gatttcggtg aagtcatgaa ctccagcaag 16981 acttcgcccc agcgtatgac cctgagcatc gtatgttcgc tggcagccgg tttttgtgcg 17041 gccagctgct atgtaacgtt ccgccggggc ttcaacggcg aagcgatgat gacgttcgac 17101 gttttcgctt tttggtatga gaccccgctt tacttgggtt atgccagcac cgtcttctgg 17161 cgtggtttat ctgttgtcat ctttacctcg ctgatcgttc tttcaagtca gctcatcata 17221 tcgctgcgca atcagaagca tcatgggaca gctcgttggg cagaaattgg cgaaatgcgg 17281 catgctggtt atctgcagcg ttacagtcgc atcaaggggc cgatctttgg aaagacatgt 17341 ggtccccttt ggttcggcag ttatttgacc aatggcgaac agccacacag tcttgtcgtc 17401 gcgccaacgc gtgctggcaa aggcgtcggc atcgtcattc caacgctgtt gaccttcaag 17461 ggctcggtaa tcgcccttga cgtcaaggga gaattgtttg aactgacgtc cagagcacgc 17521 aaagcgagcg gcgacgcagt tttcaagttc tcccccctag atcctgagcg gaagactcat 17581 tgttacaatc cggtcctgga tattgccgca cttccgcccg aacgccagtt cactgaaaca 17641 cgccgtctag ctgcgaacct tattacggct aagggaaagg gagcagaagg ctttattgac 17701 ggcgcacgtg acctgttcgt cgcgggaatc cttacctgca ttgagcgtgg cacaccaacg 17761 attggcgcgg tatatgacct atttgcgcag cctggcgaaa agtataagct ttttgcgcaa 17821 ctcgcggagg aaagcctaaa caaagaggct cagcgtatct tcgataatat ggcgggcaac 17881 gacacgaaaa ttctgacatc gtacacctct gtgctgggcg acggtggact gaacctgtgg 17941 gctgatccgc ttatcaaagc agcgacaagc cggtcagact tttccgttta cgatctccgg 18001 aggaagaaga cctgcattta tctttgtgtc agtcccaacg atctggaggt cttggcacca 18061 cttatgcgcc tgatgtttca gcagctcgtg tcaatcttgc agagatcgct gccaggtgaa 18121 gacgagtgcc atgaagtttt atttctcctc gacgaattca aacacctggg caagcttgag 18181 gccatagaga ccgcgatcac aaccatcgcc ggttacagag gccgctttat gtttattatt 18241 caaagtcttt cggccttgtc gggcacatac gatgacgcag gaaaacaaaa ctttctgagc 18301 aatactggcg tacaagtatt tatggccacg gctgatgacg aaactccaac ctacatctca 18361 aaagctatcg gcgaatatac gtttaaagcg cgttcgacct cttacagtca agccagaatg 18421 ttcgaccaca acatccagat ttctgatcaa ggtgcagccc ttttgcgccc cgaacaagtg 18481 cgcctgctag acgatcagag tgaaatcgtt ctcatcaaag ggcgacctcc actcaaatta 18541 cgaaaggtgc agtattattc cgatcgtacg ctgaaaggcc ttttcgaacg ccagatgggc 18601 tctctgcctg agcccgcacc cttgatgctt tccgactata gcaacgatca agttcaatac 18661 cacttggctc cgatagcaaa ttttaatgag gatgctgcac cgcaaaacag aactgtggcc 18721 gaggaccatg gaagtgttaa agtcggtgct gatatccctg aacgcgtgat gggaataaat 18781 ggtgacgagg aacaagccga tgcgggcgag ataccgccgg aatcggttgt gcctccagaa 18841 ttgacgctcg ctctgaccgc tcaacagcaa ttgttggacc agattattgc acttcagcaa 18901 agatcgaggt ccgcaccggc atagcctgcg aaatgatctt aatggtgcaa ttcgtttcag 18961 gcggcatgtt gcggttcaac aaagtgtacg ccagatccca attggccctt tacgaggtgt 19021 cggtatgaca ggaaagtcga aagttcacat aagaggttcg gctgacgcgc ttcctgacgt 19081 tcctggcgga agtactaccg cccctttttt aaccgaacct tctcgggatc aggttgatgc 19141 ctcgtttgag gtccaaaccg actacagcca gtctacttcc gtgtcgttta cctatgatgg 19201 tgttggactt ggtcctgccg agcgtgcggc ttacgagaac tggtgcgaac cgggccggcc 19261 cacttggaaa gatcttataa tcaaggcacg tgtcgatccg attgacgatg tgacctggct 19321 ccgagattta gaagaggaca ccccctcaac cttcagatac gaagggatgc ctctgggcat 19381 cggggaacga caggcctacg aaaattggca agaggacgct cagccgacat gggaagacct 19441 tgttgtcagc gcacgcttga cggaacttgg ccgtccacac gggattaccg gcgagtatac 19501 atccctcgca ggatcgaaga atacaagttc aatttcattg aagcgaaagc ggagcaactt 19561 aattgatgat gagaattcat ccggatcgtt ttcatatgac gggatgaagc tcggggaagc 19621 cgagcgttct gcatatggtg actgggccga ggcggagcca cccacgtgga aagatttggt 19681 attgagggca cgcgtttcct cgatcaatga ctctgcttgg ctttttgatt cacaaacatc 19741 ttcatcatca tttgaataca acggtgttcc cttgggcgag ccggaacggc aggctctcag 19801 acaatggcaa ggagacgctc agcctacctg ggaagatctc gttgttaacg cgcgtatggc 19861 agaactttgc catgctggtt ggattgaagg tcaaaaaggt tgctttgaag agcgcgggga 19921 ggctctgccc gcgtcggaac gcggttcgca acgccccatt ggtcaacgga cagattcctc 19981 cgattctttt gtgtatgatg gcacaaggct cggagcacct gagcgaactg cttatgaacg 20041 ctggagtaag agggaacgcc cgacttggga agatctcatc ttagatgcac accaggccag 20101 gactgaaagt gacgctgtta cgacccaagc gattggtcag tcgtcctcac cggttttctt 20161 atatgaagga aagtcgctcg gagacaggga acgaaaggct tacgaaaaat ggcggcagcc 20221 agcccaaccg cgatggcaaa atcttgtagt gaacgctcgt ctggcagaaa tcgatccctc 20281 agcctggatt gccgatgagc gcgatccgct tgatgatagc gacgcgcttg gtcgcccgtc 20341 gtacacaagc ttgacggata gatcagacgt ccctttagac gatcaatcaa tctatcgtcg 20401 ttccgaccta gtaagggaac aagtaccaga atcgtctcaa aggcaattcg cagcatgttc 20461 agaatctgaa acgaggcctg tgcaatggtt tactgcttct gggtcagatg caaacaatac 20521 ggaaaatatc accgccagcg atcccgtcga tcgcacgggt ggagttaagc ggctaggctc 20581 caaaagcgac agaaccgtta cagcttctat ccatgacgtg aattccagca caaggcgact 20641 gttgcttaac gaatttggat cggaggctcc gcgcccttcg ccagaaaaga ctgttcgctt 20701 aagaagcgac aatattggca cctatgggag ccggaaaaat gaacgagcgc ggctcgcgac 20761 cgaaaccggt gcgtatgagt cggagcatat tttcgggttc aaggctgtcc acgatactgc 20821 gagagcgacg aaagagggcc ggcgtctcga aaggcccatg cccgcctacc ttgaggataa 20881 ggggcttcat cgccaacata ttggcaccgg gagaggacgg accaaacttg tcgggcgcgg 20941 atggccggat gacacaagct atcgctcgga tcaaagggca actctgtcgg accccgttgc 21001 gcgctcggaa ggcgcgacgg cctcaaatgg gtatcaattg aaccaattgg gttacgcgca 21061 ccaactcgct agcgatggcc tgcaaagtga atcgcccgat ggtgttgcct tgccaattca 21121 agtggcaaca acgagctaca actatacagt gagccgcgat cctgtccttg ttccgccgga 21181 taaaaacgaa gcccctcaat tgctgcatct tggtccccgt ggtcaaaccg aagctgttct 21241 tgcccgcgaa acagcattga ctggaaaatg gccgactctc gagcgtgagc agcaagtgta 21301 tcgcgagttt ttggccttat atgacgtaaa aaaagatctt gaggccaaat cagtcggcgt 21361 aagacggaaa aaaaaagaag ttatttctgc gttagaccga actgcgcgct tgataagcac 21421 gtcgccttcg aaagctcgat ccaaagcaga gactgaaaaa gccattgatg agctcgatga 21481 tcgacgagtt tatgatccgc gtgatcgagc tcaagacaaa gcgtttaaac gctgataagt 21541 cgccaatata gtgatcattt gcagtattcg catcgatcgc tggttgatat tctgccgctg 21601 gtcgaccggc tgctcgtcgc caaaaatgct cacagggata cgatggcctc ggtcaggccg 21661 cgtgcgtcct gtctttccag ttcctccctt tcagctcgat tgtggcatca tttattgcct 21721 gctcattgca gttgaaacgc gatatccgtt tcaagacccg ggtatggatg gtactttgga 21781 gatatgagca cttgccagac ttcccggtcg gagacttaaa aattccgata agaggttacg 21841 gacgacggac cttataagtg gatcgtctag tggtggcgcc gatcaaaaca gttccgcccc 21901 cggctttgct caaagtagca aagcagcttt atctcgggtt gcggaggatt ttctagaaaa 21961 ccgcaatttt gcaggagaga acatatggcc atcatcaagc cgcatgtgaa caaaaatagg 22021 acaacctcgc cgatagaacg tccggagtct ctcatagagg aaatgagcgg cagtcatccg 22081 ccgagtggtt ttaccaacct ggatctcgct atgatcgagc tggaggactt tgtccatcgg 22141 tgcccgctcc cagaagacaa tcttgctggt cagaaggagt gagacgatgg atccgtctag 22201 caatgagaat gtctatgtgg gtcgcggtca caacatcgaa aatgatgatg acactgaccc 22261 caggcgttgg aagaaggcga atatcagttc caacaccatc tccgatattc agatgacgaa 22321 tggcgaagac gtacaatcag ggagccctac ccgaacggaa gttgtaagcc cacgtctgga 22381 ttatggatcg gtcgactcct cctccagcct ttattctggc agcgagcacg gaaatcaagc 22441 tgagattcaa aaagagctgt ccgtcttgtt ctcgaacatg tctttgccag gcaacgatcg 22501 gcgcccggac gaatacattc tcgtgcatca aacgggacaa gatgctttta ctggtattgc 22561 caaaggcaac ctcgaccaaa tgcccaccaa ggcggaattt aacgcgtgct gccgtctcta 22621 cagggacgga gccggtaatt actacccgcc acctctcgca ttcgacaaga ttagcgttcc 22681 agagcaactg gaggaaaaat gggggatgat ggaggcgaag gaacgtaaca aactgcggtt 22741 tcagtacaag ttggacgtat ggaatcatgc gcacgctgat atggggatca cgggcacaga 22801 gatcttttat caaacagata agaacataaa gctcgaccgg aattataaac taagacctga 22861 agaccgatac gtacaaacag aaaaatacgg gcgccgggaa attcaaaagc gatatcaaca 22921 cgaactccag gctggttcgc tgctgcccga tattatgatc aaaactcccc aaaatgacat 22981 ccacttcgtg tacaggtttg ccggcgacaa ttacgccaac aaacagttca gcgagtttga 23041 acacaccgtc aagcgcaggt atggcgacga gactgagatc aaattgaagt caaagtcagg 23101 cattatgcat gactcgaaat atctggaatc ctgggaacgg ggcagtgcgg atattcgctt 23161 cgcggaattc gttggggaaa atagagctca caatcggcag tttccaactg cgacagtaaa 23221 tatgggacag cagccagacg ggcagggcgg tttgacccgc gaccgtcatg tgagcgttga 23281 cttcctaatg caaagcgcac ccaattcgcc ttgggcgcaa gctttgaaaa agggagaact 23341 gtgggatcgc gttcagttgc ttgctcgcga cggcaaccgc tatctgtcgc cgcccagatt 23401 ggaatattct gaccctgcac atttcaccga gttgatgaac cgggttggtt tacccgcatc 23461 gatgggtcgg caaagccatg cggctagtat caaattcgaa aagtttgacg cgcaggcagc 23521 ggttattgtc ttaaatggcc cagagttacg tgacattcat gacttgtctc ctgaaaaact 23581 gcaaaatttg tccaccaaag atgtcatcgt cgccgatcgc aatgagaatg gtcagagaac 23641 tggcacgtac accagcgtcg cggaatatga gcgcttgcag ttaaggctgc cacccgatgc 23701 agcgggggtg cttggtgaag caactgacaa atattcacgt gatttcgttc ggccagagcc 23761 ggcgtcgcgt ccaatcagtg acagccgcag gatatacgaa agtcgaccgc gtagccaaag 23821 cgtcaacagc ttttgacgtt cctgctgccg cgtcaacgag gaagctcgtt tgacccgggt 23881 ttgccaatga aagggctcaa tcatggtgaa cactacaaag aaaagttttg cgaagtcgct 23941 tacggcagat atgcgccgtt ctgctcagcg cgttgtcgag caaatgcgaa aagcattgat 24001 taccgaagaa gaggcgctca agcggcaagc cagactggag agtcccgata ggaagcgaaa 24061 gtatgctgct gatatggcga tagtcgacaa actcgacgta gggtttcgag gcgaaatagg 24121 ctataaaatt cttggaaata accggcttcg agtagacaac cataaagaat taacgcgtga 24181 gcacggtaga cttcgcaaaa ccaaaacggt tctgaagcgt aacccggtga cgcaggaagt 24241 ctatttgggt ttatatgaaa ggaagtcctg gttaagtgtc agcagccatt tgtatgctgc 24301 ggacggcaca ctccgcatga agcacgtgaa atacaaagac ggacgttttg aggaaaaatg 24361 ggagcgcgac gaaaatggcg acctgatccg cacaaggtac gccaaccgtg gcaggctctt 24421 tcaacctgta tccgagaaaa tgggcgcgcc gtatcggagc ggccctgacg accggctcta 24481 tcgcgatcta acccgtcgaa acggtttcag acgggagaca ttcgaacggg acgatcacgg 24541 aaacctcgag cgtatcggca gcaaccatgt cggcttttcc aagatttcag tgaaggcacc 24601 caatcgtcaa acctcccaga cgaagattca aaaacttggt ggcgctttca acaaatcttt 24661 taggtccctt ctggacaagg agggcaatga aatgggccgc gatattttga gccatcgacg 24721 gctctataac aagcggtctg ctgtctacga tgaagctacc ggacaattga agagtgccaa 24781 gcataccttc ggcaagatct acaggagcga aaccgaatat ctcagcgcgg gcctcaagaa 24841 ggtttcaaaa aagatactcg gggtgacggt ctaccggaaa tttgcggcgc tcagcgagcg 24901 agaatccgag gctgagagac tgcgtagttt tgaatccggt gcgcatcgcc agatctggca 24961 ggagcgggca gcgattcccg gttcgcccct cccggagact gatgacattc atttcgcaca 25021 gcagtcgcac ctagccaaag ccaaccctga tcacgtcgaa gctgacgtca cgcgtgtgac 25081 agatcaacat gttgatgttg ctggacaaac atcatcgtct ccccaacgga acttggaagg 25141 atggttagat tctcaatcac gatacaagcc agcaaacatg ctgttgtcaa atccagacct 25201 tcaagcgaac ggacctcgcc catacgaagg gttagctcat ctcaccctcc ggcgcgataa 25261 tgaatctgac gggcacaagg agaatgatca gcggctgcga catttctccc agccagagcc 25321 gttggtgtta ccgcatcccg ggtcgccgga aataactaag gtgtttggct cgcggggaga 25381 gccggcacac ccgagtggaa atctgcacac ggcggttgga gaaacggctt gcgaaggacc 25441 ggtgatgtct tcatcctcgg acaatcatca gccagctcca ggacagcaag aacttttaag 25501 tttccttcat aatgcgccag ccccagtttc tgtggcaata catgatgatc aagagcgact 25561 tgcgggggag gcgcccggcg gctctttcag aggtagctca gggcgaacga gttcaatgtc 25621 ggagagtatc ttcgacgaag atgtacaagg gcatttggta cgggattatt cgatcaatac 25681 tactaacggg tttattgacc cgcaatcgtt gttcggtgaa ccggacttat cgagaggtcc 25741 aaaatcgggg ccagaaattc catcggaaga ttaccatttg tcagcttcgg aacaggaaaa 25801 tttgctgaat caattgctta gtgtgccact gccggttcct tcaccgaagc ccgaatgcgc 25861 gaggtctatg attttcgaag gttcacgttc aagagagcgt tccacctcca gagggttcta 25921 aggtggaacg cgtctaggct tgcctatcat caccacctca tgatggtcgc ggcttaggga 25981 taacagggta atgaattcgt gcgcagcagc tcgttatcga ccggctgcgg cgtcggggcc 26041 gccgccttgc gctgcggtcg gtgttccttc ttcggctcgc gcagcttgaa cagcatgatc 26101 gcggaaacca gcagcaacgc cgcgcctacg cctcccgcga tgtagaacag catcggattc 26161 attcttcggt cctccttgta gcggaaccgt tgtctgtgcg gcgcgggtgg cccgcgccgc 26221 tgtctttggg gatcagccct cgatgagcgc gaccagtttc acgtcggcaa ggttcgcctc 26281 gaactcctgg ccgtcgtcct cgtacttcaa ccaggcatag ccttccgccg gcggccgacg 26341 gttgaggata aggcgggcag ggcgctcgtc gtgctcgacc tggacgatgg cctttttcag 26401 cttgtccggg tccggctcct tcgcgccctt ttccttggcg tccttaccgt cctggtcgcc 26461 gtcctcgccg tcctggccgt cgccggcctc cgcgtcacgc tcggcatcag tctggccgtt 26521 gaaggcatcg acggtgttgg gatcgcggcc cttctcgtcc aggaactcgc gcagcagctt 26581 gaccgtgccg cgcgtgattt cctgggtgtc gtcgtcaagc cacgcctcga cttcctccgg 26641 gcgcttcttg aaggccgtca ccagctcgtt caccacggtc acgtcgcgca cgcggccggt 26701 gttgaacgca tcggcgatct tctccggcag gtccagcagc gtgacgtgct gggtgatgaa 26761 cgccggcgac ttgccgattt ccttggcgat atcgcctttc ttcttgccct tcgccagctc 26821 gcggccaatg aagtcggcaa tttcgcgcgg ggtcagctcg ttgcgttgca ggttctcgat 26881 aacctggtcg gcttcgttgt agtcgttgtc gatgaacgcc gggatggact tcttgccggc 26941 ccacttcgag ccacggtagc ggcgggcgcc gtgattgatg atatagcggc ccggctgctc 27001 ctggttctcg cgcaccgaaa tgggtgactt caccccgcgc tctttgatcg tggcaccgat 27061 ttccgcgatg ctctccgggg aaaagccggg gttgtcggcc gtccgcggct gatgcggatc 27121 ttcgtcgatc aggtccaggt ccagctcgat agggccggaa ccgccctgag acgccgcagg 27181 agcgtccagg aggctcgaca ggtcgccgat gctatccaac cccaggccgg acggctgcgc 27241 cgcgcctgcg gcttcctgag cggccgcagc ggtgtttttc ttggtggtct tggcttgagc 27301 cgcagtcatt gggaaatctc catcttcgtg aacacgtaat cagccagggc gcgaacctct 27361 ttcgatgcct tgcgcgcggc cgttttcttg atcttccaga ccggcacacc ggatgcgagg 27421 gcatcggcga tgctgctgcg caggccaacg gtggccggaa tcatcatctt ggggtacgcg 27481 gccagcagct cggcttggtg gcgcgcgtgg cgcggattcc gcgcatcgac cttgctgggc 27541 accatgccaa ggaattgcag cttggcgttc ttctggcgca cgttcgcaat ggtcgtgacc 27601 atcttcttga tgccctggat gctgtacgcc tcaagctcga tgggggacag cacatagtcg 27661 gccgcgaaga gggcggccgc caggccgacg ccaagggtcg gggccgtgtc gatcaggcac 27721 acgtcgaagc cttggttcgc cagggccttg atgttcgccc cgaacagctc gcgggcgtcg 27781 tccagcgaca gccgttcggc attcgccagt accgggttgg actcgatgag ggcgaggcgc 27841 gcggcctggc cgtcgccggc tgcgggtgcg gtttcggtcc agccgccggc agggacagcg 27901 ccgaacagct tgcttgcatg caggccggta gcaaagtcct tgagcgtgta ggacgcattg 27961 ccctgggggt ccaggtcgat cacggcaacc cgcaagccgc gctcgaaaaa gtcgaaggca 28021 agatgcacaa gggtcgaagt cttgccgacg ccgcctttct ggttggccgt gaccaaagtt 28081 ttcatcgttt ggtttcctgt tttttcttgg cgtccgcttc ccacttccgg acgatgtacg 28141 cctgatgttc cggcagaacc gccgttaccc gcgcgtaccc ctcgggcaag ttcttgtcct 28201 cgaacgcggc ccacacgcga tgcaccgctt gcgacactgc gcccctggtc agtcccagcg 28261 acgttgcgaa cgtcgcctgt ggcttcccat cgactaagac gccccgcgct atctcgatgg 28321 tctgctgccc cacttccagc ccctggatcg cctcctggaa ctggctttcg gtaagccgtt 28381 tcttcatgga taacacccat aatttgctcc gcgccttggt tgaacatagc ggtgacagcc 28441 gccagcacat gagagaagtt tagctaaaca tttctcgcac gtcaacacct ttagccgcta 28501 aaactcgtcc ttggcgtaac aaaacaaaag cccggaaacc gggctttcgt ctcttgccgc 28561 ttatggctct gcacccggct ccatcaccaa caggtcgcgc acgcgcttca ctcggttgcg 28621 gatcgacact gccagcccaa caaagccggt tgccgccgcc gccaggatcg cgccgatgat 28681 gccggccaca ccggccatcg cccaccaggt cgccgccttc cggttccatt cctgctggta 28741 ctgcttcgca atgctggacc tcggctcacc ataggctgac cgctcgatgg cgtatgccgc 28801 ttctcccctt ggcgtaaaac ccagcgccgc aggcggcatt gccatgctgc ccgccgcttt 28861 cccgaccacg acgcgcgcac caggcttgcg gtccagacct tcggccacgg cgagctgcgc 28921 aaggacataa tcagccgccg acttggctcc acgcgcctcg atcagctctt gcactcgcgc 28981 gaaatccttg gcctccacgg ccgccatgaa tcgcgcacgc ggcgaaggct ccgcagggcc 29041 ggcgtcgtga tcgccgccga gaatgccctt caccaagttc gacgacacga aaatcatgct 29101 gacggctatc accatcatgc agtctagata ggttaaaccc gctccgatca ggccgagcca 29161 cgccaggccg agaacattgg ttcctgtagg catcgggatt ggcggatcaa acactaaagc 29221 tactggaacg agcagaagtc ctccggccgc cagttgccag gcggtaaagg tgagcagagg 29281 cacgggaggt tgccacttgc gggtcagcac ggttccgaac gccatggaaa ccgcccccgc 29341 caggcccgct gcgacgccga caggatctag cgctgcgttt ggtgtcaaca ccaacagcgc 29401 cacgcccgca gttccgcaaa tagcccccag gaccgccatc aatcgtatcg ggctacctag 29461 cagagcggca gagatgaaca cgaccatcag cggctgcaca gcgcctaccg tcgccgcgac 29521 cccgcccggc aggcggtaga ccgaaataaa caacaagctc cagaatagcg aaatattaag 29581 tgcgccgagg atgaagatgc gcatccacca gattcccgtt ggaatctgtc ggacgatcat 29641 cacgagcaat aaacccgccg gcaacgcccg cagcagcata ccggcgaccc ctcggcctcg 29701 ctgttcgggc tccacgaaaa cgccggacag atgcgccttg tgagcgtcct tggggccgtc 29761 ctcctgtttg aagaccgaca gcccaatgat ctcgccgtcg atgtaggcgc cgaatgccac 29821 ggcatctcgc aaccgttcag cgaacgcctc catgggcttt ttctcctcgt gctcgtaaac 29881 ggacccgaac atctctggag ctttcttcag ggccgacaat cggatctcgc ggaaatcctg 29941 cacgtcggcc gctccaagcc gtcgaatctg agccttaatc acaattgtca attttaatcc 30001 tctgtttatc ggcagttcgt agagcgcgcc gtgcgtcccg agcgatactg agcgaagcaa 30061 gtgcgtcgag cagtgcccgc ttgttcctga aatgccagta aagcgctggc tgctgaaccc 30121 ccagccggaa ctgaccccac aaggccctag cgtttgcaat gcaccaggtc atcattgacc 30181 caggcgtgtt ccaccaggcc gctgcctcgc aactcttcgc aggcttcgcc gacctgctcg 30241 cgccacttct tcacgcgggt ggaatccgat ccgcacatga ggcggaaggt ttccagcttg 30301 agcgggtacg gctcccggtg cgagctgaaa tagtcgaaca tccgtcgggc cgtcggcgac 30361 agcttgcggt acttctccca tatgaatttc gtgtagtggt cgccagcaaa cagcacgacg 30421 atttcctcgt cgatcaggac ctggcaacgg gacgttttct tgccacggtc caggacgcgg 30481 aagcggtgca gcagcgacac cgattccagg tgcccaacgc ggtcggacgt gaagcccatc 30541 gccgtcgcct gtaggcgcga caggcattcc tcggccttcg tgtaataccg gccattgatc 30601 gaccagccca ggtcctggca aagctcgtag aacgtgaagg tgatcggctc gccgataggg 30661 gtgcgcttcg cgtactccaa cacctgctgc cacaccagtt cgtcatcgtc ggcccgcagc 30721 tcgacgccgg tgtaggtgat cttcacgtcc ttgttgacgt ggaaaatgac cttgttttgc 30781 agcgcctcgc gcgggatttt cttgttgcgc gtggtgaaca gggcagagcg ggccgtgtcg 30841 tttggcatcg ctcgcatcgt gtccggccac ggcgcaatat cgaacaagga aagctgcatt 30901 tccttgatct gctgcttcgt gtgtttcagc aacgcggcct gcttggcctc gctgacctgt 30961 tttgccaggt cctcgccggc ggtttttcgc ttcttggtcg tcatagttcc tcgcgtgtcg 31021 atggtcatcg acttcgccaa acctgccgcc tcctgttcga gacgacgcga acgctccacg 31081 gcggccgatg gcgcgggcag ggcaggggga gccagttgca cgctgtcgcg ctcgatcttg 31141 gccgtagctt gctggaccat cgagccgacg gactggaagg tttcgcgggg cgcacgcatg 31201 acggtgcggc ttgcgatggt ttcggcatcc tcggcggaaa accccgcgtc gatcagttct 31261 tgcctgtatg ccttccggtc aaacgtccga ttcattcacc ctccttgcgg gattgccccg 31321 actcacgccg gggcaatgtg cccttattcc tgatttgacc cgcctggtgc cttggtgtcc 31381 agataatcca ccttatcggc aatgaagtcg gtcccgtaga ccgtctggcc gtccttcccg 31441 tacttggtat tccgaatctt gccctgcacg aataccagcg accccttgcc caaatacttg 31501 ccgtgggcct cggcctgaga gccaaaacac ttgatgcgga agaagtcggt gcgctcctgc 31561 ttgtcgccgg catcgttgcg ccactcttca ttaaccgcta tatcgaaaat tgcttgcggc 31621 ttgttagaat tgccatgacg tacctcggtg tcacgggtaa gattaccgat aaactggaac 31681 tgattatggc tcatatcgaa agtctccttg agaaaggaga ctctagttta gctaaacatt 31741 ggttccgctg tcaagaactt tagcggctaa aattttgcgg gccgcgacca aaggtgcgag 31801 gggcggcttc cgctgtgtac aaccagatat ttttcaccaa catccttcgt ctgctcgatg 31861 agcggggcat gacgaaacat gagctgtcgg agagggcagg ggtttcaatt tcgtttttat 31921 cagacttaac caacggtaag gccaacccct cgttgaaggt gatggaggcc attgccgacg 31981 ccctggaaac tcccctacct cttctcctgg agtccaccga ccttgaccgc gaggcactcg 32041 cggagattgc gggtcatcct ttcaagagca gcgtgccgcc cggatacgaa cgcatcagtg 32101 tggttttgcc gtcacataag gcgtttatcg taaagaaatg gggcgacgac acccgaaaaa 32161 agctgcgtgg aaggctctga cgccaagggt tagggcttgc acttccttct ttagccgcta 32221 aaacggcccc ttctctgcgg gccgtcggct cgcgcatcat atcgacatcc tcaacggaag 32281 ccgtgccgcg aatggcatcg ggcgggtgcg ctttgacagt tgttttctat cagaacccct 32341 acgtcgtgcg gttcgattaa gcttggacct aaagtcgtta attaagtacg ccatcaggac 32401 gttgtgagtg gcgcgatttt tagcggctga aatcagccct tgagcctgtc ggcaagtcgc 32461 gtcatgaggt ccatgcgctc atgcaggatc gccacgacca acgcgggttc gcccgcacgc 32521 ggcaggcaaa aaacgtagtg gtgttcgcag cgggccatcc gcagcgcggg aaagagttcg 32581 ctcatgtcct taaacgggcc ttcgccggcg gcaagcctgg ctatgccctg ttccagctta 32641 gcgatatagc ggcgcacctg cgccgcgccc cactcccggc gcgtgtagcg gatgatgccg 32701 cgtagatcgg cttcggcctc agccgtgagg atgtaggccg tcaagcgcga tccccgctga 32761 gttcttcatc aagaatttcg ccgacgctct tggtggacac cttgccggca agcccatcgt 32821 tgatgcggtt ccccagcatg gttttcagtt cctgccatgc ctgatcggca tcagcgtcac 32881 cggggaacag acgttcgagg gcgtattgct taatggtctt gccctgcaag gcggccaggg 32941 ctttcaggct ctggtgctgc tggtccgtca tgtcgattgt caggcggctc attggataac 33001 ctccataaaa tacacgtaac cacattagca catatgtggg cgtgaggcta cagcgcgagg 33061 cgcattaagg tcgggaaaat gcgctaggcg catttaaatt gcgtattgct gtaatgcgcc 33121 atgccggcta gactaggccc aaatgggtat acccaatttg accaaggggg acgccgatga 33181 attaagctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat 33241 gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct 33301 tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc 33361 catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca 33421 ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga 33481 gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg 33541 ccctggcgac gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct 33601 actggacatt gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc 33661 gtgggccgac accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc 33721 cgagttcgag cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc 33781 ccgaggcgtg aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg 33841 cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca 33901 tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag 33961 gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga 34021 gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt 34081 ttcattaccg aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc 34141 gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg 34201 gcggcctggc cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga 34261 tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta 34321 aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg 34381 ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg 34441 atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg 34501 aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg 34561 ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg 34621 tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca 34681 tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa 34741 ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg 34801 ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg 34861 tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg 34921 acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta 34981 atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc 35041 acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt 35101 caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg 35161 caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa 35221 atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac 35281 aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg 35341 taagcggctg ggttgtctgc cggccctgca atggcactgg aacccccaag cccgaggaat 35401 cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga 35461 cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc 35521 acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc 35581 gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt 35641 tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc 35701 cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc 35761 agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga 35821 cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa 35881 gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg 35941 gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac 36001 cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc 36061 cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga 36121 gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc 36181 ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct 36241 ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat 36301 ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct 36361 gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc 36421 gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg 36481 tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggtctctt 36541 tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta 36601 cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa 36661 agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac 36721 ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc 36781 tacccttcgg tcgctgtgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc 36841 tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc 36901 gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg 36961 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 37021 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg 37081 gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc 37141 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt 37201 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 37261 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 37321 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 37381 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 37441 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 37501 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 37561 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 37621 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 37681 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 37741 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 37801 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 37861 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 37921 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 37981 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 38041 cgaaaactca cgttaaggga ttttggtcat gcatgatata tctcccaatt tgtgtaggcg 38101 cgccttgggc ttattatgca cgcttaaaaa taataaaagc agacttgacc tgatagtttg 38161 gctgtgagca attatgtgct tagtgcatct aacgcttgag ttaagccgcg ccgcgaagcg 38221 gcgtcggctt gaacgaattt ctagctagac attatttgcc gactaccttg gtgatctcgc 38281 ctttcacgta gtggacaaat tcttccaact gatctgcgcg cgaggccaag cgatcttctt 38341 cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg gccggcaggc 38401 gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt actgcgctgt 38461 accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag tcgggcggcg 38521 agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca ggaaccggat 38581 caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt gcttttgtca 38641 gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca agaatgtcat 38701 tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac ggaatgatgt 38761 cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct ccaggggaag 38821 ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg 38881 tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc actgcggagc 38941 cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg ccaactacct 39001 ctgatagttg agtcgatact tcggcgatca ccgcttcccc catgatgttt aactttgttt 39061 tagggcgact gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca 39121 cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt 39181 cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc 39241 tggaccagtt gcgtgagcgc atacgctact tgcattacag cttacgaacc g //
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇:pCNA
- 下一篇:pTetQCas-8+IS186