| 质粒名称: | pTwist_Lenti_SFFV-ActA(1-584)-BFP-FKBP |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 213119 |
| 存储实验室: | Scott Coyle |
|
载体骨架基本信息 |
|
| 载体名称: | pTwist SFFV |
| 载体出品公司: | Twist |
| 空载体大小: | 5683bp |
| 完整质粒大小: | 8527bp |
| 载体类型: | 哺乳细胞表达, Lentiviral |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | ActA(1-584)-BFP-FKBP |
| 物种来源: | Listeria monocytogenes |
| 插入大小: | 2844bp |
| 启动子: | SFFV |
| 克隆方法: | 未知 |
| 5’测序引物 | N/A |
| 3’测序引物 | N/A |
|
文献引用方法 |
|
| 材料和方法部分: |
pTwist_Lenti_SFFV-ActA(1-584)-BFP-FKBP was a gift from Scott Coyle (Addgene plasmid # 213119 ; http://n2t.net/addgene:213119 ; RRID:Addgene_213119)
|
| 参考文献部分: | A programmable reaction-diffusion system for spatiotemporal cell signaling circuit design. Rajasekaran R, Chang CC, Weix EWZ, Galateo TM, Coyle SM. Cell. 2023 Dec 27:S0092-8674(23)01339-9. doi: 10.1016/j.cell.2023.12.007. 10.1016/j.cell.2023.12.007 PubMed 38181787 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
-BFP-FKBP质粒图谱.png)
载体简介
载体序列
LOCUS pTwist_Lenti_SFFV-ActA(1-584)-BFP-FKBP 8527 bp ds-DNA circular SYN 26-JAN-2024
DEFINITION Expression of an ActA FKBP payload for MinDE recruitment circuits in
mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pTwist_Lenti_SFFV-ActA(1-584)-BFP-FKBP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8527)
AUTHORS Rajasekaran R, Chang CC, Weix EWZ, Galateo TM, Coyle SM
TITLE A programmable reaction-diffusion system for spatiotemporal cell
signaling circuit design.
JOURNAL Cell. 2023 Dec 27:S0092-8674(23)01339-9. doi:
10.1016/j.cell.2023.12.007.
PUBMED 38181787
REFERENCE 2 (bases 1 to 8527)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jan 26, 2024 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8527
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 249..293
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 560..677
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
regulatory 1225..1234
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 3013..3711
/codon_start=1
/product="monomeric blue fluorescent protein"
/label=TagBFP
/note="mammalian codon-optimized"
/translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
DLPSKLGHKLN"
CDS 3745..4068
/codon_start=1
/product="human FK506-binding protein FKBP12"
/label=FKBP
/translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
KLE"
LTR 4211..4444
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
rep_origin 4705..4840
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4767..4786
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
rep_origin complement(4958..5546)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5038..5057)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS 5908..6768
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6126..6145)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
terminator 6781..6917
/label=rrnG terminator
/note="transcription terminator from the E. coli ribosomal
RNA rrnG operon (Albrechtsen et al., 1991)"
enhancer 6994..7297
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 7298..7501
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 7452..7472
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 7516..7696
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 7740..7865
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 8358..64
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
ORIGIN
1 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
61 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
121 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
181 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
241 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
301 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
361 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
421 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
481 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
541 ctcgacggta tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg
601 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
661 caaaaattca aaattttggc tcccgatcgt tgcgttacac acacaattac tgctgatcga
721 gtgtagcctt cgaatgaaag accccacctg taggtttggc aagatagctg cagtaacgcc
781 attttgcaag gcatggaaaa ataccaaacc aagaatagag aagttcagat caagggcggg
841 tacatgaaaa tagctaacgt tgggccaaac aggatatctg cggtgagcag tttcggcccc
901 ggcccggggc caagaacaga tggtcaccgc agtttcggcc ccggcccgag gccaagaaca
961 gatggtcccc agatatggcc caaccctcag cagtttctta agacccatca gatgtttcca
1021 ggctccccca aggacctgaa atgaccctgc gccttatttg aattaaccaa tcagcctgct
1081 tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat aaaagagctc acaacccctc
1141 actcggcgcg ccagtcctcc gattgactga gtcgccctga tcattgtcga tcctaccatc
1201 cactcgacac acccgccagg atccgccacc atgggactga acagatttat gcgggccatg
1261 atggtggttt tcattactgc caactgcatt acgattaacc ccgacataat attcgcagcg
1321 actgacagcg aagattctag tttgaacaca gacgaatggg aggaggaaaa gacagaagag
1381 cagcccagcg aggtgaatac cggaccacgc tacgaaactg cacgtgaggt gagttcacgg
1441 gatattaaag aactcgagaa aagcaataag gtgagaaaca ctaacaaagc agacctaatc
1501 gcaatgttga aagaaaaagc cgagaaaggt ccaaacatca ataataacaa cagtgaacag
1561 actgagaatg ccgctataaa tgaagaggct tcaggagccg accgacccgc tatccaggtg
1621 gagcgtcggc accctggcct gccctccgac agcgccgccg aaattaaaaa gaggaggaaa
1681 gccatcgcat cctcggatag tgagcttgaa agcctgactt atccggataa accaacaaaa
1741 gtaaataaga agaaggtggc gaaggagtca gttgccgatg cttctgaaag tgacttagac
1801 tctagcatgc agtcagcaga cgagtcttcc ccacaacctc tgaaagccaa ccaacagccc
1861 ttcttcccta aggtctttaa aaaaataaag gatgccggga aatgggtccg cgacaagatc
1921 gacgaaaatc ctgaggtcaa gaaggcgatt gttgataaat ccgccgggct catcgaccag
1981 ctgctcacca agaagaagag tgaggaggtg aatgcttctg acttcccgcc cccacctacg
2041 gatgaagagc tgcgactggc tttgccagag acccctatgc ttctggggtt taatgctcct
2101 gccacatcag aaccgagctc attcgagttt ccaccacccc ctaccgacga agagctgagg
2161 cttgctttgc ccgagacgcc aatgctgctt ggttttaatg cccctgccac ctccgagccc
2221 agctcgttcg aattcccacc tcctcccact gaggatgaac tcgagatcat ccgggagaca
2281 gcatcctctc tcgattcttc ctttacccgc ggggacttag cttccttgcg aaatgctatt
2341 aaccgccatt cccaaaattt ctctgacttc ccacctatcc ccacagaaga ggagttgaac
2401 gggaggggcg gcagaccaac atctgaggag ttctcctcgc tgaatagtgg tgattttacc
2461 gatgacgaga acagcgagac aacagaagaa gaaattgatc gcctagccga tctgagagat
2521 agaggaacag gaaaacactc aaggaatgcg ggatttctgc ccctgaatcc gttcgccagc
2581 agcccggttc cttctttaag tcccaaggtc tccaaaatca gcgcaccggc tctgatcagt
2641 gacataacta aaaagacgcc atttaagaat ccttcccagc ccctgaatgt gtttaataag
2701 aagactacca ctaagaccgt gactaagaaa cctacccctg tgaagaccgc cccaaagcta
2761 gcagaacttc ctgccacaaa gccccaggag accgtactta gggaaaacaa gacccccttt
2821 atagagaagc aggcagagac aaacaagcag tcaatcaaca tgcccagcct ccccgtcatc
2881 cagaaagagg ctaccgagag cgataaggag gagatgaaac cacaaaccga ggaaaagatg
2941 gtagaggaaa gcgaatcagc caacaacgca aacggcaaga acggtagcgg cggaagcggt
3001 ggcggtggca gcatgagcga gctgattaag gagaacatgc acatgaagct gtacatggag
3061 ggcaccgtgg acaaccatca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag
3121 ggcacccaga ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac
3181 atcctggcta ctagcttcct ctacggcagc aagaccttca tcaaccacac ccagggcatc
3241 cccgacttct tcaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac
3301 gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc
3361 tacaacgtca agatcagagg ggtgaacttc acatccaacg gccctgtgat gcagaagaaa
3421 acactcggct gggaggcctt caccgagacg ctgtaccccg ctgacggcgg cctggaaggc
3481 agaaacgaca tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa catcaagacc
3541 acatatagat ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac
3601 tacagactgg aaagaatcaa ggaggccaac aacgagacct acgtcgagca gcacgaggtg
3661 gcagtggcca gatactgcga cctccctagc aaactggggc acaagcttaa tgacagcgcc
3721 ggaagtgctg gctctgcggg taccggagtg caggtggaaa ccatctcccc aggagacggg
3781 cgcaccttcc ccaagcgcgg ccagacctgc gtggtgcact acaccgggat gcttgaagat
3841 ggaaagaaat ttgattcctc ccgggacaga aacaagccct ttaagtttat gctaggcaag
3901 caggaggtga tccgaggctg ggaagaaggg gttgcccaga tgagtgtggg tcagagagcc
3961 aaactgacta tatctccaga ttatgcctat ggtgccactg ggcacccagg catcatccca
4021 ccacatgcca ctctcgtctt cgatgtggag cttctaaaac tggaatgact cgagggcccg
4081 catccaccat cgcagactta tcatcttatt ccagatgcgt gcggatggaa ttcgagctcg
4141 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
4201 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
4261 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
4321 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
4381 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
4441 agcagtcctg gccaacgtga gcaccgtgct gacctccaaa tatcgttaag ctggagcctg
4501 ggagccggcc tggccctccg ccccccccac ccccgcagcc cacccctggt ctttgaataa
4561 agtctgagtg agtggccgac agtgcccgtg gagttctcgt gacctgaggt gcagggccgg
4621 cgctagggac acgtccgtgc acgtgccgag gccccctgtg cagctgcaag ggacaggcct
4681 agccctgcag gcctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
4741 ccgcctcatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
4801 gagctattcc agaagtagtg aggacgcttt tttggaggcc gaggcttttg caaagatcga
4861 acaagagaca ggacctgcag gttaattaaa tttaaatcat gtgagcaaaa ggccagcaaa
4921 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
4981 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
5041 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
5101 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
5161 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
5221 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
5281 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
5341 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
5401 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
5461 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
5521 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
5581 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
5641 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
5701 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
5761 tatttcgttc atccatagtt gcatttaaat ggccggcctg gcgcgccgtt taaacctaga
5821 tattgatagt ctgatcggtc aacgtataat cgagtcctag cttttgcaaa catctatcaa
5881 gagacaggat cagcaggagg ctttcgcatg agtattcaac atttccgtgt cgcccttatt
5941 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
6001 aaagatgctg aagatcagtt gggtgcgcga gtgggttaca tcgaactgga tctcaacagc
6061 ggtaagatcc ttgagagttt tcgccccgaa gaacgctttc caatgatgag cacttttaaa
6121 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
6181 cgcatacact attctcagaa tgacttggtt gagtattcac cagtcacaga aaagcatctt
6241 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
6301 gcggccaact tacttctgac aacgattgga ggaccgaagg agctaaccgc ttttttgcac
6361 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
6421 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacctt gcgtaaacta
6481 ttaactggcg aactacttac tctagcttcc cggcaacagt tgatagactg gatggaggcg
6541 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
6601 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
6661 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
6721 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaacc gattctaggt
6781 gcattggcgc agaaaaaaat gcctgatgcg acgctgcgcg tcttatactc ccacatatgc
6841 cagattcagc aacggatacg gcttccccaa cttgcccact tccatacgtg tcctccttac
6901 cagaaattta tccttaagat cccgaatcgt ttaaacgcga tcgcagtaat caattacggg
6961 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
7021 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
7081 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
7141 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
7201 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
7261 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
7321 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
7381 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
7441 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
7501 tcgtttagtg aaccggggtc tctctggtta gaccagatct gagcctggga gctctctggc
7561 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
7621 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
7681 tggaaaatct ctagcagtgg cgcccgaaca gggacctgaa agcgaaaggg aaaccagagc
7741 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
7801 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
7861 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
7921 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
7981 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
8041 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
8101 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
8161 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
8221 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
8281 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
8341 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
8401 atgggcgcag cctcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
8461 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
8521 gtctggg
//
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇：pLV-EF1a-PKAsub-mCherry-MinD-IRES-Puro
- 下一篇：没有了
