| 出品公司: | BD Biosciences |
|---|---|
| 载体名称: | pAcG2T |
| 质粒类型: | 昆虫细胞载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | -- |
| 启动子: | -- |
| 载体大小: | 8530 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pAcG2T 8530 bp ds-DNA circular SYN 19-APR-2014
DEFINITION Baculovirus transfer vector for expressing N-terminally GST-tagged
proteins with a thrombin cleavage site, for use with the BD
BaculoGold(TM) system.
ACCESSION .
VERSION .
KEYWORDS pAcG2T
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8530)
AUTHORS BD Biosciences
TITLE Direct Submission
JOURNAL Exported Wednesday, February 5, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8530
/organism="synthetic DNA construct"
/lab_host="Insect Cells"
/mol_type="other DNA"
misc_recomb 1269..2096
/note="baculovirus recombination region (lef2/ORF603)"
/note="contains ORF603 and part of lef2"
CDS 1269..1393
/codon_start=3
/product="baculovirus late expression factor 2"
/note="lef2"
/translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
CDS complement(1431..2036)
/codon_start=1
/product="baculovirus ORF603 protein"
/note="ORF603"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
promoter 2100..2191
/gene="polh from Autographa californica"
/note="polyhedrin promoter"
/note="promoter for the baculovirus polyhedrin gene"
CDS 2206..2859
/codon_start=1
/product="glutathione S-transferase from Schistosoma
japonicum"
/note="GST"
/translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
CDS 2866..2883
/codon_start=1
/product="thrombin recognition and cleavage site"
/note="thrombin site"
/translation="LVPRGS"
misc_feature 2878..2893
/note="MCS"
/note="multiple cloning site"
misc_recomb 2923..4280
/note="baculovirus recombination region (ORF1629)"
/note="contains part of ORF1629"
CDS complement(2939..4280)
/codon_start=2
/product="baculovirus capsid-associated protein"
/note="ORF1629"
/note="required for viral replication"
/translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
rep_origin 5653..6108
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 6390..6494
/gene="bla"
/note="AmpR promoter"
CDS 6495..7355
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 7526..8114
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tcgagcagtt cgttgacgcc ttcctccgtg tggccgaaca cgtcgagcgg gtggtcgatg
61 accagcggcg tgccgcacgc gacgcacaag tatctgtaca ccgaatgatc gtcgggcgaa
121 ggcacgtcgg cctccaagtg gcaatattgg caaattcgaa aatatataca gttgggttgt
181 ttgcgcatat ctatcgtggc gttgggcatg tacgtccgaa cgttgatttg catgcaagcc
241 gaaattaaat cattgcgatt agtgcgatta aaacgttgta catcctcgct tttaatcatg
301 ccgtcgatta aatcgcgcaa tcgagtcaag tgatcaaagt gtggaataat gttttctttg
361 tattcccgag tcaagcgcag cgcgtatttt aacaaactag ccatcttgta agttagtttc
421 atttaatgca actttatcca ataatatatt atgtatcgca cgtcaagaat taacaatgcg
481 cccgttgtcg catctcaaca cgactatgat agagatcaaa taaagcgcga attaaatagc
541 ttgcgacgca acgtgcacga tctgtgcacg cgttccggca cgagctttga ttgtaataag
601 tttttacgaa gcgatgacat gacccccgta gtgacaacga tcacgcccaa aagaactgcc
661 gactacaaaa ttaccgagta tgtcggtgac gttaaaacta ttaagccatc caatcgaccg
721 ttagtcgaat caggaccgct ggtgcgagaa gccgcgaagt atggcgaatg catcgtataa
781 cgtgtggagt ccgctcatta gagcgtcatg tttagacaag aaagctacat atttaattga
841 tcccgatgat tttattgata aattgaccct aactccatac acggtattct acaatggcgg
901 ggttttggtc aaaatttccg gactgcgatt gtacatgctg ttaacggctc cgcccactat
961 taatgaaatt aaaaattcca attttaaaaa acgcagcaag agaaacattt gtatgaaaga
1021 atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac aacaagatta atatgcctcc
1081 gtgtataaaa aaaatattga acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta
1141 caggaagagg tttatactaa actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga
1201 aaaccgatgt ttaatcaagg ctctgacgca tttctacaac cacgactcca agtgtgtggg
1261 tgaagtcatg catcttttaa tcaaatccca agatgtgtat aaaccaccaa actgccaaaa
1321 aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat
1381 ttgtaattat tgaataataa aacaattata aatgctaaat ttgtttttta ttaacgatac
1441 aaaccaaacg caacaagaac atttgtagta ttatctataa ttgaaaacgc gtagttataa
1501 tcgctgaggt aatatttaaa atcattttca aatgattcac agttaatttg cgacaatata
1561 attttatttt cacataaact agacgccttg tcgtcttctt cttcgtattc cttctctttt
1621 tcatttttct cctcataaaa attaacatag ttattatcgt atccatatat gtatctatcg
1681 tatagagtaa attttttgtt gtcataaata tatatgtctt ttttaatggg gtgtatagta
1741 ccgctgcgca tagtttttct gtaatttaca acagtgctat tttctggtag ttcttcggag
1801 tgtgttgctt taattattaa atttatataa tcaatgaatt tgggatcgtc ggttttgtac
1861 aatatgttgc cggcatagta cgcagcttct tctagttcaa ttacaccatt ttttagcagc
1921 accggattaa cataactttc caaaatgttg tacgaaccgt taaacaaaaa cagttcacct
1981 cccttttcta tactattgtc tgcgagcagt tgtttgttgt taaaaataac agccattgta
2041 atgagacgca caaactaata tcacaaactg gaaatgtcta tcaatatata gttgctgata
2101 tcatggagat aattaaaatg ataaccatct cgcaaataaa taagtatttt actgttttcg
2161 taacagtttt gtaataaaaa aacctataaa tacggatctg tattcatgtc ccctatacta
2221 ggttattgga aaattaaggg ccttgtgcaa cccactcgac ttcttttgga atatcttgaa
2281 gaaaaatatg aagagcattt gtatgagcgc gatgaaggtg ataaatggcg aaacaaaaag
2341 tttgaattgg gtttggagtt tcccaatctt ccttattata ttgatggtga tgttaaatta
2401 acacagtcta tggccatcat acgttatata gctgacaagc acaacatgtt gggtggttgt
2461 ccaaaagagc gtgcagagat ttcaatgctt gaaggagcgg ttttggatat tagatacggt
2521 gtttcgagaa ttgcatatag taaagacttt gaaactctca aagttgattt tcttagcaag
2581 ctacctgaaa tgctgaaaat gttcgaagat cgtttatgtc ataaaacata tttaaatggt
2641 gatcatgtaa cccatcctga cttcatgttg tatgacgctc ttgatgttgt tttatacatg
2701 gacccaatgt gcctggatgc gttcccaaaa ttagtttgtt ttaaaaaacg tattgaagct
2761 atcccacaaa ttgataagta cttgaaatcc agcaagtata tagcatggcc tttgcagggc
2821 tggcaagcca cgtttggtgg tggcgaccat cctccaaaat cggatctggt tccgcgtgga
2881 tccccgggaa ttcatcgtga ctgactgacg atctgagatc cggttattag tacatttatt
2941 aagcgctaga ttctgtgcgt tgttgattta cagacaattg ttgtacgtat tttaataatt
3001 cattaaattt ataatcttta gggtggtatg ttagagcgaa aatcaaatga ttttcagcgt
3061 ctttatatct gaatttaaat attaaatcct caatagattt gtaaaatagg tttcgattag
3121 tttcaaacaa gggttgtttt tccgaaccga tggctggact atctaatgga ttttcgctca
3181 acgccacaaa acttgccaaa tcttgtagca gcaatctagc tttgtcgata ttcgtttgtg
3241 ttttgttttg taataaaggt tcgacgtcgt tcaaaatatt atgcgctttt gtatttcttt
3301 catcactgtc gttagtgtac aattgactcg acgtaaacac gttaaataaa gcttggacat
3361 atttaacatc gggcgtgtta gctttattag gccgattatc gtcgtcgtcc caaccctcgt
3421 cgttagaagt tgcttccgaa gacgattttg ccatagccac acgacgccta ttaattgtgt
3481 cggctaacac gtccgcgatc aaatttgtag ttgagctttt tggaattatt tctgattgcg
3541 ggcgtttttg ggcgggtttc aatctaactg tgcccgattt taattcagac aacacgttag
3601 aaagcgatgg tgcaggcggt ggtaacattt cagacggcaa atctactaat ggcggcggtg
3661 gtggagctga tgataaatct accatcggtg gaggcgcagg cggggctggc ggcggaggcg
3721 gaggcggagg tggtggcggt gatgcagacg gcggtttagg ctcaaatgtc tctttaggca
3781 acacagtcgg cacctcaact attgtactgg tttcgggcgc cgtttttggt ttgaccggtc
3841 tgagacgagt gcgatttttt tcgtttctaa tagcttccaa caattgttgt ctgtcgtcta
3901 aaggtgcagc gggttgaggt tccgtcggca ttggtggagc gggcggcaat tcagacatcg
3961 atggtggtgg tggtggtgga ggcgctggaa tgttaggcac gggagaaggt ggtggcggcg
4021 gtgccgccgg tataatttgt tctggtttag tttgttcgcg cacgattgtg ggcaccggcg
4081 caggcgccgc tggctgcaca acggaaggtc gtctgcttcg aggcagcgct tggggtggtg
4141 gcaattcaat attataattg gaatacaaat cgtaaaaatc tgctataagc attgtaattt
4201 cgctatcgtt taccgtgccg atatttaaca accgctcaat gtaagcaatt gtattgtaaa
4261 gagattgtct caagctcgga tcgatcccgc acgccgataa caagcctttt catttttact
4321 acagcattgt agtggcgaga cacttcgctg tcgtcgacgt acatgtatgc tttgttgtca
4381 aaaacgtcgt tggcaagctt taaaatattt aaaagaacat ctctgttcag caccactgtg
4441 ttgtcgtaaa tgttgttttt gataatttgc gcttccgcag tatcgacacg ttcaaaaaat
4501 tgatgcgcat caattttgtt gttcctatta ttgaataaat aagattgtac agattcatat
4561 ctacgattcg tcatggccac cacaaatgct acgctgcaaa cgctggtaca attttacgaa
4621 aactgcaaaa acgtcaaaac tcggtataaa ataatcaacg ggcgctttgg caaaatatct
4681 attttatcgc acaagcccac tagcaaattg tatttgcaga aaacaatttc ggcgcacaat
4741 tttaacgctg acgaaataaa agttcaccag ttaatgagcg accacccaaa ttttataaaa
4801 atctatttta atcacggttc catcaacaac caagtgatcg tgatggacta cattgactgt
4861 cccgatttat ttgaaacact acaaattaaa ggcgagcttt cgtaccaact tgttagcaat
4921 attattagac agctgtgtga agcgctcaac gatttgcaca agcacaattt catacacaac
4981 gacataaaac tcgaaaatgt cttatatttc gaagcacttg atcgcgtgta tgtttgcgat
5041 tacggattgt gcaaacacga aaactcactt agcgtgcacg acggcacgtt ggagtatttt
5101 agtccggaaa aaattcgaca cacaactatg cacgtttcgt ttgactggta cgcggcgtgt
5161 taacatacaa gttgctaacc ggcggccgac acccatttga aaaaagcgaa gacgaaatgt
5221 tggacttgaa tagcatgaag cgtcgtcagc aatacaatga cattggcgtt ttaaaacacg
5281 ttcgtaacgt taacgctcgt gactttgtgt actgcctaac aagatacaac atagattgta
5341 gactcacaaa ttacaaacaa attataaaac atgagttttt gtcgtaaaaa tgccacttgt
5401 tttacgagta gaattagctt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac
5461 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
5521 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
5581 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
5641 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
5701 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
5761 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
5821 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
5881 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
5941 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcgggctatt
6001 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
6061 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
6121 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
6181 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
6241 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
6301 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
6361 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
6421 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
6481 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
6541 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
6601 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
6661 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
6721 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
6781 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
6841 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
6901 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
6961 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
7021 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
7081 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
7141 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
7201 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
7261 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
7321 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
7381 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
7441 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
7501 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
7561 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
7621 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
7681 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
7741 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
7801 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
7861 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
7921 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
7981 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
8041 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
8101 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
8161 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
8221 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
8281 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
8341 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
8401 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
8461 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
8521 attacgaatt
//
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