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pLOX-CW-CRE载体质粒基本信息
pLOX-CW-CRE质粒图谱载体图谱和pLOX-CW-CRE载体序列质粒序列多克隆位点信息
pLOX-CW-CRE质粒载体简介
pLOX-CW-CRE质粒序列载体序列
LOCUS pLOX-CW-CRE 9886 bp DNA SYN DEFINITION pLOX-CW-CRE ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..9886 /organism="pLOX-CW-CRE" /mol_type="other DNA" misc_feature 457..637 /label="HIV-1_5_LTR" misc_feature 457..637 /label="truncHIV-1_3_LTR" misc_feature 748..792 /label="HIV-1_psi_pack" CDS 1180..2028 /label="ORF frame 1" /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN GSRRYR*" misc_feature 1302..1535 /label="RRE" /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN GSRRYR*" promoter 2040..2544 /label="CMV_immearly_promoter" /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN GSRRYR*" misc_feature 2047..2334 /label="CAG_enhancer" /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN GSRRYR*" misc_feature 2501..2521 /label="CMV_fwd_primer" /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN GSRRYR*" promoter 2502..2571 /label="CMV_promoter" /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN GSRRYR*" misc_feature 2545..2564 /label="pCEP_fwd_primer" /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN GSRRYR*" misc_feature 2547..2571 /label="LNCX_primer" /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN GSRRYR*" CDS 2640..3692 /label="ORF frame 3" /translation="MPKKKRKVSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAF SEHTWKMLLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQL NMLHRRSGLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSD RCQDIRNLAFLGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEK ALSLGVTKLVERWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATH RLIYGAKDDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRN LDSETGAMVRLLEDGD*" misc_feature 2643..2663 /label="SV40 NLS" /translation="MPKKKRKVSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAF SEHTWKMLLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQL NMLHRRSGLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSD RCQDIRNLAFLGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEK ALSLGVTKLVERWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATH RLIYGAKDDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRN LDSETGAMVRLLEDGD*" misc_feature 2649..3692 /label="CRE_NLS" /translation="MPKKKRKVSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAF SEHTWKMLLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQL NMLHRRSGLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSD RCQDIRNLAFLGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEK ALSLGVTKLVERWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATH RLIYGAKDDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRN LDSETGAMVRLLEDGD*" misc_feature 2649..2663 /label="NLS" /translation="MPKKKRKVSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAF SEHTWKMLLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQL NMLHRRSGLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSD RCQDIRNLAFLGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEK ALSLGVTKLVERWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATH RLIYGAKDDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRN LDSETGAMVRLLEDGD*" CDS 3682..4368 /label="ORF frame 1" /translation="MAIRSSSSFEFDNQPLDYKICERLTGILNYVAPFTLCGYAALMP LYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTCQL LSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPWLLACVATWIL RGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPHREFELGTFK TNDLQGSCRS*" misc_feature 3720..4312 /label="WPRE" /translation="MAIRSSSSFEFDNQPLDYKICERLTGILNYVAPFTLCGYAALMP LYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTCQL LSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPWLLACVATWIL RGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPHREFELGTFK TNDLQGSCRS*" CDS complement(3805..4308) /label="ORF frame 2" /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS NSMIQRH*" misc_feature 4378..4399 /label="U3PPT" /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS NSMIQRH*" misc_feature 4378..4393 /label="cPPT" /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS NSMIQRH*" misc_feature 4450..4483 /label="loxP" /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS NSMIQRH*" misc_feature 4503..4683 /label="HIV-1_5_LTR" /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS NSMIQRH*" misc_feature 4503..4683 /label="truncHIV-1_3_LTR" /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS NSMIQRH*" misc_feature complement(4709..4726) /label="Sp6_primer" /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS NSMIQRH*" gene 4964..5227 /label="tet (300 - 563)" /gene="tet (300 - 563)" /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS NSMIQRH*" misc_feature complement(5016..5035) /label="pBRrevBam_primer" /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS NSMIQRH*" misc_feature 5368..5390 /label="pGEX_3_primer" /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS NSMIQRH*" promoter 5549..5577 /label="AmpR_promoter" /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS NSMIQRH*" gene 5619..6479 /label="Ampicillin" /gene="Ampicillin" /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS NSMIQRH*" CDS 5619..6479 /label="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" rep_origin 6634..7253 /label="pBR322_origin" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" misc_feature complement(7479..7499) /label="pBABE_3_primer" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" misc_feature complement(7485..7938) /label="SV40_enhancer" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" promoter 7497..7765 /label="SV40_promoter" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" rep_origin 7664..7741 /label="SV40_origin" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" misc_feature 7726..7745 /label="SV40pro_F_primer" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" misc_feature 8946..8961 /label="SV40_int" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" misc_feature 8967..9014 /label="SV40_3_splice" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" CDS 9091..9564 /label="ORF frame 1" /translation="MPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFS NRTLACFAIYTTKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINN YAQKLCTFSFLICKGVNKEYLMYSALTRDHNQPYHICRGFTCFKKPPTPPPEPET*" misc_feature 9142..9162 /label="SV40 NLS" /translation="MPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFS NRTLACFAIYTTKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINN YAQKLCTFSFLICKGVNKEYLMYSALTRDHNQPYHICRGFTCFKKPPTPPPEPET*" misc_feature 9148..9162 /label="NLS" /translation="MPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFS NRTLACFAIYTTKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINN YAQKLCTFSFLICKGVNKEYLMYSALTRDHNQPYHICRGFTCFKKPPTPPPEPET*" terminator 9590..9721 /label="SV40_PA_terminator" /translation="MPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFS NRTLACFAIYTTKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINN YAQKLCTFSFLICKGVNKEYLMYSALTRDHNQPYHICRGFTCFKKPPTPPPEPET*" misc_feature 9678..9697 /label="EBV_rev_primer" /translation="MPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFS NRTLACFAIYTTKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINN YAQKLCTFSFLICKGVNKEYLMYSALTRDHNQPYHICRGFTCFKKPPTPPPEPET*" ORIGIN 1 agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc 61 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc 121 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg 181 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc 241 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc 301 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt 361 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca 421 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc 481 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg 541 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc 601 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga 661 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca 721 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct 781 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat 841 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat 901 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag 961 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta 1021 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag 1081 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac 1141 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt 1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca 1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg 1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc 1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg 1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg 1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa 1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag 1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta 1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg 1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat 1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta 1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc 1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac 1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgataagc ttgggagttc 2041 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca 2101 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt 2161 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg 2221 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag 2281 tacatgacct tacgggactt tcctacttgg cagtacatct acgtattagt catcgctatt 2341 accatggtga tgcggttttg gcagtacacc aatgggcgtg gatagcggtt tgactcacgg 2401 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa 2461 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt 2521 gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga 2581 cgccatccac gctgttttga cctccataga agacaccgac tctagctaga ggatctacca 2641 tgcccaagaa gaagaggaag gtgtccaatt tactgaccgt acaccaaaat ttgcctgcat 2701 taccggtcga tgcaacgagt gatgaggttc gcaagaacct gatggacatg ttcagggatc 2761 gccaggcgtt ttctgagcat acctggaaaa tgcttctgtc cgtttgccgg tcgtgggcgg 2821 catggtgcaa gttgaataac cggaaatggt ttcccgcaga acctgaagat gttcgcgatt 2881 atcttctata tcttcaggcg cgcggtctgg cagtaaaaac tatccagcaa catttgggcc 2941 agctaaacat gcttcatcgt cggtccgggc tgccacgacc aagtgacagc aatgctgttt 3001 cactggttat gcggcggatc cgaaaagaaa acgttgatgc cggtgaacgt gcaaaacagg 3061 ctctagcgtt cgaacgcact gatttcgacc aggttcgttc actcatggaa aatagcgatc 3121 gctgccagga tatacgtaat ctggcatttc tggggattgc ttataacacc ctgttacgta 3181 tagccgaaat tgccaggatc agggttaaag atatctcacg tactgacggt gggagaatgt 3241 taatccatat tggcagaacg aaaacgctgg ttagcaccgc aggtgtagag aaggcactta 3301 gcctgggggt aactaaactg gtcgagcgat ggatttccgt ctctggtgta gctgatgatc 3361 cgaataacta cctgttttgc cgggtcagaa aaaatggtgt tgccgcgcca tctgccacca 3421 gccagctatc aactcgcgcc ctggaaggga tttttgaagc aactcatcga ttgatttacg 3481 gcgctaagga tgactctggt cagagatacc tggcctggtc tggacacagt gcccgtgtcg 3541 gagccgcgcg agatatggcc cgcgctggag tttcaatacc ggagatcatg caagctggtg 3601 gctggaccaa tgtaaatatt gtcatgaact atatccgtaa cctggatagt gaaacagggg 3661 caatggtgcg cctgctggaa gatggcgatt aggtcgagct caagcttcga attcgataat 3721 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct 3781 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg 3841 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg 3901 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt 3961 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt 4021 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg 4081 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc 4141 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat 4201 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc 4261 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ggaattcgag 4321 ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa 4381 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatg ggatcaattc 4441 accatgggaa taacttcgta tagcatacat tatacgaagt tatgctgctt tttgcttgta 4501 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc 4561 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt 4621 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta 4681 gcagcatcta gaattaattc cgtgtattct atagtgtcac ctaaatcgta tgtgtatgat 4741 acataaggtt atgtattaat tgtagccgcg ttctaacgac aatatgtaca agcctaattg 4801 tgtagcatct ggcttactga agcagaccct atcatctctc tcgtaaactg ccgtcagagt 4861 cggtttggtt ggacgaacct tctgagtttc tggtaacgcc gtcccgcacc cggaaatggt 4921 cagcgaacca atcagcaggg tcatcgctag ccagatcctc tacgccggac gcatcgtggc 4981 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg 5041 ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc 5101 aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc 5161 ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa 5221 gggagagcgt cgaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc 5281 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat 5341 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt 5401 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg 5461 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa 5521 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac 5581 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg 5641 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc 5701 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg 5761 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga 5821 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc 5881 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag 5941 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga 6001 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg 6061 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga 6121 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt 6181 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact 6241 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt 6301 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg 6361 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta 6421 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac 6481 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta 6541 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt 6601 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt 6661 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt 6721 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc 6781 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg 6841 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg 6901 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt 6961 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac 7021 tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg 7081 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg 7141 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat 7201 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt 7261 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg 7321 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa 7381 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc 7441 ctctccccgc gcgttggccg attcattaat gcagctgtgg aatgtgtgtc agttagggtg 7501 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc 7561 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 7621 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc 7681 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc 7741 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct 7801 aggcttttgc aaaaagcttg gacacaagac aggcttgcga gatatgtttg agaataccac 7861 tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg 7921 tttttagtgc gccagatctc tataatctcg cgcaacctat tttcccctcg aacacttttt 7981 aagccgtaga taaacaggct gggacacttc acatgagcga aaaatacatc gtcacctggg 8041 acatgttgca gatccatgca cgtaaactcg caagccgact gatgccttct gaacaatgga 8101 aaggcattat tgccgtaagc cgtggcggtc tgtaccgggt gcgttactgg cgcgtgaact 8161 gggtattcgt catgtcgata ccgtttgtat ttccagctac gatcacgaca accagcgcga 8221 gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct 8281 ggtggatacc ggtggtactg cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt 8341 caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat gactatgttg ttgatatccc 8401 gcaagatacc tggattgaac agccgtggga tatgggcgtc gtattcgtcc cgccaatctc 8461 cggtcgctaa tcttttcaac gcctggcact gccgggcgtt gttcttttta acttcaggcg 8521 ggttacaata gtttccagta agtattctgg aggctgcatc catgacacag gcaaacctga 8581 gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta gtccgccgct 8641 ttaatcacgg cgcacaaccg cctgtgcagt cggcccttga tggtaaaacc atccctcact 8701 ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc 8761 tcgacgtcca ggcacgtatt gtgatgagcg atgccgaacg taccgacgat gatttatacg 8821 atacggtgat tggctaccgt ggcggcaact ggatttatga gtgggccccg gatctttgtg 8881 aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc 8941 tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt 9001 tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt 9061 aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct 9121 gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt 9181 ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc 9241 tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa 9301 tattctgtaa cctttataag taggcataac agttataatc ataacatact gttttttctt 9361 actccacaca ggcatagagt gtctgctatt aataactatg ctcaaaaatt gtgtaccttt 9421 agctttttaa tttgtaaagg ggttaataag gaatatttga tgtatagtgc cttgactaga 9481 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca 9541 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc 9601 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 9661 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat 9721 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac 9781 cactgccaat tacctagtgg tttcatttac tctaaacctg tgattcctct gaattatttt 9841 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagt //
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