| 质粒名称: | shh10 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 64867 |
| 存储实验室: | John Flannery, David Schaffer |
|
载体骨架基本信息 |
|
| 载体名称: | XX2 |
| 载体出品公司: | University of North Carolina Dr. Samulski |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5648 bp |
| 完整质粒大小: | 8182 bp |
| 载体修饰: | replaced AAV2 cap with shh10 cap |
| 载体类型: | 腺病毒表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | shh10 cap |
| 物种来源: | Adeno-associated virus - 6 |
| 插入大小: | 2211 bp |
| 基因突变: | I319V, N451D, D532N |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | HindIII(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | GCGGAAGCTTCGATCAACTACGC |
| 3' 测序引物 | GCAGTGCCAGGGTTGATTATAG |
|
文献引用方法 |
|
| 材料和方法部分: | shh10 was a gift from John Flannery & David Schaffer (Addgene plasmid # 64867 ; http://n2t.net/addgene:64867 ; RRID:Addgene_64867) |
| 参考文献部分: | A novel adeno-associated viral variant for efficient and selective intravitreal transduction of rat Muller cells. Klimczak RR, Koerber JT, Dalkara D, Flannery JG, Schaffer DV. PLoS One. 2009 Oct 14;4(10):e7467. doi: 10.1371/journal.pone.0007467. 10.1371/journal.pone.0007467 PubMed 19826483 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS shh10 8342 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION This plasmid can be used in the triple transfection method of AAV
vector production. It supplies the replication proteins from AAV2
and the engineered shh10 capsid protein..
ACCESSION .
VERSION .
KEYWORDS shh10
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8342)
AUTHORS Klimczak RR, Koerber JT, Dalkara D, Flannery JG, Schaffer DV
TITLE A novel adeno-associated viral variant for efficient and selective
intravitreal transduction of rat Muller cells.
JOURNAL PLoS One. 2009 Oct 14;4(10):e7467. doi:
10.1371/journal.pone.0007467.
PUBMED 19826483
REFERENCE 2 (bases 1 to 8342)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8342
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(30..52)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 152..171
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
rep_origin complement(653..1166)
/direction=LEFT
/label=M13 ori
/note="M13 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(848..869)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 1060..1079
/label=F1ori-R
/note="F1 origin, reverse primer"
repeat_region 6179..6281
/label=ITR
/note="inverted terminal repeat of human adenovirus
serotype 5"
primer_bind complement(6353..6370)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6524..7112)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6604..6623)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7283..8143)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7906..7925
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8144..8248)
/gene="bla"
/label=AmpR promoter
primer_bind 8316..8334
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
61 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
121 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
181 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcga
241 ttccaacatc caataaatca tacaggcaag gcaaagaatt agcaaaatta agcaataaag
301 cctcagagca taaagctaaa tcggttgtac caaaaacatt atgaccctgt aatacttttg
361 cgggagaagc ctttatttca acgcaaggat aaaaattttt agaaccctca tatattttaa
421 atgcaatgcc tgagtaatgt gtaggtaaag attcaaacgg gtgagaaagg ccggagacag
481 tcaaatcacc atcaatatga tattcaaccg ttctagctga taaattcatg ccggagaggg
541 tagctatttt tgagaggtct ctacaaaggc tatcaggtca ttgcctgaga gtctggagca
601 aacaagagaa tcgatgaacg gtaatcgtaa aactagcatg tcaatcatat gtaccccggt
661 tgataatcag aaaagcccca aaaacaggaa gattgtataa gcaaatattt aaattgtaag
721 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
781 atatgccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
841 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
901 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
961 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
1021 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
1081 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
1141 gcttaatgcg ccgctacagg gcgcgtacta tggttgcttt gacgagcacg tataacgtgc
1201 tttcctcgtt agaatcagag cgggagctaa acaggaggcc gattaaaggg attttagaca
1261 ggaacggtac gccagaatcc tgagaagtgt ttttataatc agtgaggcca ccgagtaaaa
1321 gagtctgtcc atcacgcaaa ttaaccgttg tcgcaatact tctttgatta gtaataacat
1381 cacttgcctg agtagaagaa ctcaaactat cggccttgct ggtaatatcc agaacaatat
1441 taccgccagc cattgcaacg gaatcgccat tcgccattca ggctgcgcaa ctgttgggaa
1501 gggcgatcgg tgcgggcctc ttcgctatta cgccagcgat gcagcttctc ggccacggtc
1561 aggggtgcct gctcaatcag attcagatcc atgtcagaat ctggcggcaa ctcccattcc
1621 ttctcggcca cccagttcac aaagctgtca gaaatgccgg gcagatgctc gtcaaggtcg
1681 ctggggacct taatcacaat ctcgtaaaac cccgccgtgg tggctgcgcg ttcaaacctc
1741 ccgcttcaaa atggagaccc tgcgtgctca ctcgggctta aatacccagc gtgaccacat
1801 ggtgtcgcaa aatgtcgcaa aacactcacg tgacctctaa tacaggacct ctagagcatg
1861 gaaactagat aagaaagaaa tacgcagaga ccaaagttca actgaaacga attaaacggt
1921 ttattgatta acaagcggcc gcaattacag gggacgggtg aggtaacggg tgccaatggg
1981 gcgaggctca gtataaagtc cattgttgtc cacagtgaaa tcaacgttgg cagattttgc
2041 atagttagat gtatactgca cttcgggatt ccagcgtttg ctgttttctt tctgcagctc
2101 ccattcaatc tccacgctca cttgtcctgt ggaatactgg gtgatgaatg aagcaaactt
2161 tgtagccgaa aactctgccg gaggattcgc aggaacaggc gtgtttttga tgaggatctg
2221 aggaggcggg tgcttaagtc caaagccgcc catgagagga gacgggtgaa agtgtccatc
2281 cgtgtgagga attttggccc aaataggacc ctgcaggtat acgtctctgt cttgccacac
2341 cattccaggt aaggctccca taacatgcac atctccggtc gcagggtctg tgctgctgct
2401 ctggagattg actgccacag tcccaaatct ttcggtggcc acggggttag tggctttgat
2461 ttcctcttcg tctgtgatca tgacattgtc caatgcagtg tttgaagctc cggcgctctc
2521 ctttccaaaa atcatgacac cgctcatggg aaagaacttg tttttgtcgt ctttgtgtga
2581 ggccatagca gtgccagggt tgattataga ttcacgccca ttaaggttat attttgaagc
2641 accagtccag gtaaagttgc tgttgttgtt gtctgttttt gttttagaaa cgcgctgctg
2701 ccggtaacag ggtccaggta gccagttttt gggctgaaca gacatgccag ctggagaccc
2761 ccggctaaac agcaagtcct tgttttgggc acttccggac tgatcctgag ttctgttcag
2821 gtaatacagg tactggtcga tgagaggatt catcagccgg tccaggctct ggctgtgcgc
2881 gtagctgctg tggaaaggca cgtcctcgaa ggtgtagctg aaggtaaagt tattgcccgt
2941 tctcagcatc tgcgatggga aatattccag gcagtaaaag gatgaccgtc ccactgcctg
3001 gctgccattg ttgagcgtta ggtagccgta ctgcggaatc atgaacacgt ccgccgggaa
3061 cggagggagg cagccctggt gcgcagagcc gaggacgtac ggcaactggt actccgagtc
3121 cgagaagact tgaaccgtgc tggtaaggtt attagcgatg gtcgtgacgc catcattcgt
3181 cgtgacctcc ttgacttgga cgttgaagag cttgaagttg agtctcttgg gccggaatcc
3241 ccaattgttg ttgatgagtc gctgccagtc acgtggtgag aaatggcagt ggaatctgtt
3301 gaaatcaaaa tacccccagg gggtgctgta gccgaagtag tggttgtcgt tgctggcccc
3361 cgttgaagca ctggagattt gcttgtagag gtggttgtta taggtgggca aggcccatgt
3421 tcgggtgctg gtggtgatga ctctgtcgcc cagccatgtg gaatcgcaat gccaatttcc
3481 tgaggcatta cccactccgt cggcgccttc gttattgtct gccattggtg cgccaccgcc
3541 tgaagccatt gtagtaggtc ccacagcagc gggggttgct ggaggttctc cgagaggttg
3601 tgggtcgggg actgactctg agtcgccagt ctgaccaaaa ttgagtctct ttttagcggg
3661 ctgctggcct gtcttgccaa tgcccgagga ggagtctggc tcttgtggcg actgctctac
3721 cggacgtttc tttccaggag ccgtcttagc accttcctca accagaccaa aaggttcgag
3781 aaccctcttc ttggcctgga agactgctcg cccgaggttg cccccaaaag acgtatcttc
3841 ttgcagacgc tcctgaaact cggcgtcggc gtggttatac cgcaggtacg gattgtcacc
3901 cgctttgagc tgctggtcgt aggccttgtc gtgctcgagg gccgctgcat ccgccgcgtt
3961 gacgggctcc cccttgtcga gtccgttgaa gggtccgagg tacttgtagc caggaagcac
4021 cagaccccgg ccgtcgtcct gcttttgctg gttggctttg ggtttcgggg ctccaggttt
4081 caagtcccac cactcgcgaa tgccctcaga gaggttgtcc tcaagccaat ctggaagata
4141 accatcggca gccatacctg atttaaatca tttattgttc aaagatgcag tcatccaaat
4201 ccacattgac cagatcgcag gcagtgcaag cgtctggcac ctttcccatg atatgatgaa
4261 tgtagcacag tttctgatac gcctttttga cgacagaaac gggttgagat tctgacacgg
4321 gaaagcactc taaacagtct ttctgtccgt gagtgaagca gatatttgaa ttctgattca
4381 ttctctcgca ttgtctgcag ggaaacagca tcagattcat gcccacgtga cgagaacatt
4441 tgttttggta cctgtctgcg tagttgatcg aagcttccgc gtctgacgtc gatggctgcg
4501 caactgactc gcgcacccgt ttgggctcac ttatatctgc gtcactgggg gcgggtcttt
4561 tcttggctcc accctttttg acgtagaatt catgctccac ctcaaccacg tgatcctttg
4621 cccaccggaa aaagtctttg acttcctgct tggtgacctt cccaaagtca tgatccagac
4681 ggcgggtgag ttcaaatttg aacatccggt cttgcaacgg ctgctggtgt tcgaaggtcg
4741 ttgagttccc gtcaatcacg gcgcacatgt tggtgttgga ggtgacgatc acgggagtcg
4801 ggtctatctg ggccgaggac ttgcatttct ggtccacgcg caccttgctt cctccgagaa
4861 tggctttggc cgactccacg accttggcgg tcatcttccc ctcctcccac cagatcacca
4921 tcttgtcgac acagtcgttg aagggaaagt tctcattggt ccagtttacg cacccgtaga
4981 agggcacagt gtgggctatg gcctccgcga tgttggtctt cccggtagtt gcaggcccaa
5041 acagccagat ggtgttcctc ttgccgaact ttttcgtggc ccatcccaga aagacggaag
5101 ccgcatattg gggatcgtac ccgtttagtt ccaaaatttt ataaatccga ttgctggaaa
5161 tgtcctccac gggctgctgg cccaccaggt agtcgggggc ggttttagtc aggctcataa
5221 tctttcccgc attgtccaag gcagccttga tttgggaccg cgagttggag gccgcattga
5281 aggagatgta tgaggcctgg tcctcctgga tccactgctt ctccgaggta atccccttgt
5341 ccacgagcca cccgaccagc tccatgtacc tggctgaagt ttttgatctg atcaccggcg
5401 catcagaatt gggattctga ttctctttgt tctgctcctg cgtctgcgac acgtgcgtca
5461 gatgctgcgc caccaaccgt ttacgctccg tgagattcaa acaggcgctt aaatactgtt
5521 ccatattagt ccacgcccac tggagctcag gctgggtttt ggggagcaag taattgggga
5581 tgtagcactc atccaccacc ttgttcccgc ctccggcgcc atttctggtc tttgtgaccg
5641 cgaaccagtt tggcaaagtc ggctcgatcc cgcggtaaat tctctgaatc agtttttcgc
5701 gaatctgact caggaaacgt cccaaaacca tggatttcac cccggtggtt tccacgagca
5761 cgtgcatgtg gaagtagctc tctcccttct caaattgcac aaagaaaagg gcctccgggg
5821 ccttactcac acggcgccat tccgtcagaa agtcgcgctg cagcttctcg gccacggtca
5881 ggggtgcctg ctcaatcaga ttcagatcca tgtcagaatc tggcggcaac tcccattcct
5941 tctcggccac ccagttcaca aagctgtcag aaatgccggg cagatgctcg tcaaggtcgc
6001 tggggacctt aatcacaatc tcgtaaaacc ccgccgtggt ggctgcgcgt tcaaacctcc
6061 cgcttcaaaa tggagaccct gcgtgctcac tcgggcttaa atacccagcg tgaccacatg
6121 gtgtcgcaaa atgtcgcaaa acactcacgt gacctctaat acaggacctc tagagctact
6181 acgtcacccg ccccgttccc acgccccgcg ccacgtcaca aactccaccc cctcattatc
6241 atattggctt caatccaaaa taaggtatat tattgatgat gggaattctg cattaatgaa
6301 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
6361 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
6421 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
6481 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
6541 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
6601 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
6661 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
6721 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
6781 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
6841 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
6901 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
6961 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
7021 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
7081 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
7141 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
7201 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
7261 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
7321 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
7381 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
7441 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
7501 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
7561 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
7621 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
7681 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
7741 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
7801 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
7861 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
7921 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
7981 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
8041 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
8101 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
8161 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
8221 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
8281 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
8341 gt
//
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