| 质粒名称: | pSFV-Helper1 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 92073 |
| 存储实验室: | Henrik Garoff |
|
载体骨架基本信息 |
|
| 载体名称: | pGEM1 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 2833 bp |
| 完整质粒大小: | 8194 bp |
| 载体修饰: | -- |
| 载体类型: | 辅助质粒，用于哺乳动物细胞的转录和RNA转染 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | cDNA of SFV genome with deletion removing the nonstructural SFV proteins and also the viral RNA packaging signal. |
| 物种来源: | -- |
| 插入大小: | 5361 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pSFV-Helper1 was a gift from Henrik Garoff (Addgene plasmid # 92073 ; http://n2t.net/addgene:92073 ; RRID:Addgene_92073) |
| 参考文献部分: | A new generation of animal cell expression vectors based on the Semliki Forest virus replicon. Liljestrom P, Garoff H. Biotechnology (N Y). 1991 Dec;9(12):1356-61. PubMed 1370252 |
买家导航
pSFVHelper1载体基本信息
pSFVHelper1载体图谱质粒图谱和多克隆位点信息
pSFVHelper1载体简介
pSFVHelper1载体序列
LOCUS pSFV-Helper1 8192 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION This is a plasmid for transcription of defective helper RNA,
providing structural Semliki forest virus (SFV) proteins to package
recombinant SFV genomes into virus like particles..
ACCESSION .
VERSION .
KEYWORDS pSFV-Helper1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8192)
AUTHORS Liljestrom P, Garoff H
TITLE A new generation of animal cell expression vectors based on the
Semliki Forest virus replicon.
JOURNAL Biotechnology (N Y). 1991 Dec;9(12):1356-61.
PUBMED 1370252
REFERENCE 2 (bases 1 to 8192)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8192
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..19
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 1..18
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(5510..5527)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5681..6269)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5761..5780)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6440..7300)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7063..7082
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7301..7405)
/gene="bla"
/label=AmpR promoter
primer_bind 7473..7491
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(7529..7551)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 7651..7670
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 7884..7903
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
ORIGIN
1 atttaggtga cactatagat ggcggatgtg tgacatacac gacgccaaaa gattttgttc
61 cagctcctgc cacctccgct acgcgagaga ttaaccaccc acgatggccg ccaaagtgca
121 tgttgatatt gaggctgaca gcccattcat caagtctttg cagaaggcat ttccgtcgtt
181 cgaggtggag tcattgcagg tcacaccaaa tgaccatgca aatgccagag cattttcgca
241 cctggctacc aaattgatcg agcaggagac tgacaaagac acactcatct tggatatcgg
301 cagtgcgcct tccaggagaa tgatgtcgac atgaaacgag atgtcaaagt cactccaggg
361 acgaaacaca cagaggaaag acccaaagtc caggtaattc aagcagcgga gccattggcg
421 accgcttacc tgtgcggcat ccacagggaa ttagtaagga gactaaatgc tgtgttacgc
481 cctaacgtgc acacattgtt tgatatgtcg gccgaagact ttgacgcgat catcgcctct
541 cacttccacc caggagaccc ggttctagag acggacattg catcattcga caaaagccag
601 gacgactcct tggctcttac aggtttaatg atcctcgaag atctaggggt ggatcagtac
661 ctgctggact tgatcgaggc agcctttggg gaaatatcca gctgtcacct accaactggc
721 acgcgcttca agttcggagc tatgatgaaa tcgggcatgt ttctgacttt gtttattaac
781 actgttttga acatcaccat agcaagcagg gtactggagc agagactcac tgactccgcc
841 tgtgcggcct tcatcggcga cgacaacatc gttcacggag tgatctccga caagctgatg
901 gcggagaggt gcgcgtcgtg ggtcaacatg gaggtgaaga tcattgacgc tgtcatgggc
961 gaaaaacccc catatttttg tgggggattc atagtttttg acagcgtcac acagaccgcc
1021 tgccgtgttt cagacccact taagcgcctg ttcaagttgg gtaagccgct aacagctgaa
1081 gacaagcagg acgaagacag gcgacgagca ctgagtgacg aggttagcaa gtggttccgg
1141 acaggcttgg gggccgaact ggaggtggca ctaacatcta ggtatgaggt agagggctgc
1201 aaaagtatcc tcatagccat ggccaccttg gcgagggaca ttaaggcgtt taagaaattg
1261 agaggacctg ttatacacct ctacggcggt cctagattgg tgcgttaata cacagaattc
1321 tgattatagc gcactattat agcaccatga attacatccc tacgcaaacg ttttacggcc
1381 gccggtggcg cccgcgcccg gcggcccgtc cctggccgtt gcaggccact ccggtggctc
1441 ccgtcgtccc cgacttccag gcccagcaga tgcagcaact catcagcgcc gtaaatgcgc
1501 tgacaatgag acagaacgca attgctcctg ctaggcctcc caaaccaaag aagaagaaga
1561 caaccaaacc aaagccgaaa acgcagccca agaagatcaa cggaaaaacg cagcagcaaa
1621 agaagaaaga caagcaagcc gacaagaaga agaagaaacc cggaaaaaga gaaagaatgt
1681 gcatgaagat tgaaaatgac tgtatcttcg aagtcaaaca cgaaggaaag gtcactgggt
1741 acgcctgcct ggtgggcgac aaagtcatga aacctgccca cgtgaaagga gtcatcgaca
1801 acgcggacct ggcaaagcta gctttcaaga aatcgagcaa gtatgacctt gagtgtgccc
1861 agataccagt tcacatgagg tcggatgcct caaagtacac gcatgagaag cccgagggac
1921 actataactg gcaccacggg gctgttcagt acagcggagg taggttcact ataccgacag
1981 gagcgggcaa accgggagac agtggccggc ccatctttga caacaagggt agggtagtcg
2041 ctatcgtcct gggcggggcc aacgagggct cacgcacagc actgtcggtg gtcacctgga
2101 acaaagatat ggtgactaga gtgacccccg aggggtccga agagtggtcc gccccgctga
2161 ttactgccat gtgtgtcctt gccaatgcta ccttcccgtg cttccagccc ccgtgtgtac
2221 cttgctgcta tgaaaacaac gcagaggcca cactacggat gctcgaggat aacgtggata
2281 ggccagggta ctacgacctc cttcaggcag ccttgacgtg ccgaaacgga acaagacacc
2341 ggcgcagcgt gtcgcaacac ttcaacgtgt ataaggctac acgcccttac atcgcgtact
2401 gcgccgactg cggagcaggg cactcgtgtc atagccccgt agcaattgaa gcggtcaggt
2461 ccgaagctac cgacgggatg ctgaagattc agttctcggc acaaattggc atagataaga
2521 gtgacaatca tgactacacg aagataaggt acgcagacgg gcacgccatt gagaatgccg
2581 tccggtcatc tttgaaggta gccacctccg gagactgttt cgtccatggc acaatgggac
2641 atttcatact ggcaaagtgc ccaccgggtg aattcctgca ggtctcgatc caggacacca
2701 gaaacgcggt ccgtgcctgc agaatacaat atcatcatga ccctcaaccg gtgggtagag
2761 aaaaatttac aattagacca cactatggaa aagagatccc ttgcaccact tatcaacaga
2821 ccacagcgaa gaccgtggag gaaatcgaca tgcatatgcc gccagatacg ccggacagga
2881 cgttgctatc acagcaatct ggcaatgtaa agatcacagt cggaggaaag aaggtgaaat
2941 acaactgcac ctgtggaacc ggaaacgttg gcactactaa ttcggacatg acgatcaaca
3001 cgtgtctaat agagcagtgc cacgtctcag tgacggacca taagaaatgg cagttcaact
3061 cacctttcgt cccgagagcc gacgaaccgg ctagaaaagg caaagtccat atcccattcc
3121 cgttggacaa catcacatgc agagttccaa tggcgcgcga accaaccgtc atccacggca
3181 aaagagaagt gacactgcac cttcacccag atcatcccac gctcttttcc taccgcacac
3241 tgggtgagga cccgcagtat cacgaggaat gggtgacagc ggcggtggaa cggaccatac
3301 ccgtaccagt ggacgggatg gagtaccact ggggaaacaa cgacccagtg aggctttggt
3361 ctcaactcac cactgaaggg aaaccgcacg gctggccgca tcagatcgta cagtactact
3421 atgggcttta cccggccgct acagtatccg cggtcgtcgg gatgagctta ctggcgttga
3481 tatcgatctt cgcgtcgtgc tacatgctgg ttgcggcccg cagtaagtgc ttgacccctt
3541 atgctttaac accaggagct gcagttccgt ggacgctggg gatactctgc tgcgccccgc
3601 gggcgcacgc agctagtgtg gcagagacta tggcctactt gtgggaccaa aaccaagcgt
3661 tgttctggtt ggagtttgcg gcccctgttg cctgcatcct catcatcacg tattgcctca
3721 gaaacgtgct gtgttgctgt aagagccttt cttttttagt gctactgagc ctcggggcaa
3781 ccgccagagc ttacgaacat tcgacagtaa tgccgaacgt ggtggggttc ccgtataagg
3841 ctcacattga aaggccagga tatagccccc tcactttgca gatgcaggtt gttgaaacca
3901 gcctcgaacc aacccttaat ttggaataca taacctgtga gtacaagacg gtcgtcccgt
3961 cgccgtacgt gaagtgctgc ggcgcctcag agtgctccac taaagagaag cctgactacc
4021 aatgcaaggt ttacacaggc gtgtacccgt tcatgtgggg aggggcatat tgcttctgcg
4081 actcagaaaa cacgcaactc agcgaggcgt acgtcgatcg atcggacgta tgcaggcatg
4141 atcacgcatc tgcttacaaa gcccatacag catcgctgaa ggccaaagtg agggttatgt
4201 acggcaacgt aaaccagact gtggatgttt acgtgaacgg agaccatgcc gtcacgatag
4261 ggggtactca gttcatattc gggccgctgt catcggcctg gaccccgttc gacaacaaga
4321 tagtcgtgta caaagacgaa gtgttcaatc aggacttccc gccgtacgga tctgggcaac
4381 cagggcgctt cggcgacatc caaagcagaa cagtggagag taacgacctg tacgcgaaca
4441 cggcactgaa gctggcacgc ccttcacccg gcatggtcca tgtaccgtac acacagacac
4501 cttcagggtt caaatattgg ctaaaggaaa aagggacagc cctaaatacg aaggctcctt
4561 ttggctgcca aatcaaaacg aaccctgtca gggccatgaa ctgcgccgtg ggaaacatcc
4621 ctgtctccat gaatttgcct gacagcgcct ttacccgcat tgtcgaggcg ccgaccatca
4681 ttgacctgac ttgcacagtg gctacctgta cgcactcctc ggatttcggc ggcgtcttga
4741 cactgacgta caagaccgac aagaacgggg actgctctgt acactcgcac tctaacgtag
4801 ctactctaca ggaggccaca gcaaaagtga agacagcagg taaggtgacc ttacacttct
4861 ccacggcaag cgcatcacct tcttttgtgg tgtcgctatg cagtgctagg gccacctgtt
4921 cagcgtcgtg tgagcccccg aaagaccaca tagtcccata tgcggctagc cacagtaacg
4981 tagtgtttcc agacatgtcg ggcaccgcac tatcatgggt gcagaaaatc tcgggtggtc
5041 tgggggcctt cgcaatcggc gctatcctgg tgctggttgt ggtcacttgc attgggctcc
5101 gcagataagt tagggtaggc aatggcattg atatagcaag aaaattgaaa acagaaaaag
5161 ttagggtaag caatggcata taaccataac tgtataactt gtaacaaagc gcaacaagac
5221 ctgcgcaatt ggccccgtgg tccgcctcac ggaaactcgg ggcaactcat attgacacat
5281 taattggcaa taattggaag cttacataag cttaattcga cgaataattg gatttttatt
5341 ttattttgca attggttttt aatatttcca aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
5401 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaac tagtctgcat taatgaatcg
5461 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
5521 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
5581 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
5641 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
5701 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
5761 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
5821 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
5881 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
5941 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
6001 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
6061 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
6121 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
6181 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
6241 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
6301 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
6361 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
6421 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
6481 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
6541 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
6601 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
6661 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
6721 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
6781 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
6841 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
6901 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
6961 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
7021 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
7081 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
7141 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
7201 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
7261 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
7321 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
7381 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
7441 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
7501 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
7561 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
7621 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
7681 accattcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg
7741 aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt
7801 cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag
7861 tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct
7921 gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatctggct agcgatgacc
7981 ctgctgattg gttcgctgac catttccggg tgcgggacgg cgttaccaga aactcagaag
8041 gttcgtccaa ccaaaccgac tctgacggca gtttacgaga gagatgatag ggtctgcttc
8101 agtaagccag atgctacaca attaggcttg tacatattgt cgttagaacg cggctacaat
8161 taatacataa ccttatgtat catacacata cg
//
