| 质粒名称: | pTH100 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 84458 |
| 存储实验室: | Reindert Nijland |
|
载体骨架基本信息 |
|
| 载体名称: | pJB38-NWMN2930 |
| 空载体大小: | 8815 bp |
| 完整质粒大小: | 9969 bp |
| 筛选标记: | 也含有氯霉素抗性基因，但仅在革兰氏阳性细菌中起作用。 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | E.coli DC10b (described in doi: 10.1128/mBio.00537-12) |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | SarA_P1-sGFP from pCM29 |
| 插入大小: | 1154 bp |
| 标签/融合蛋白: | 6xHis（在sfGFP上）（插入件上的C端子） |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | EcoRV（在克隆过程中被破坏） |
| 3’克隆位点: | EcoRV（在克隆过程中被破坏） |
| 5’测序引物 | CATAATGTGTTGTAAACATTTTTTTTG |
| 3’测序引物 | TATGTCACTTATCCTTTTGGAAATG |
|
文献引用方法 |
|
| 材料和方法部分: | pTH100 was a gift from Reindert Nijland (Addgene plasmid # 84458 ; http://n2t.net/addgene:84458 ; RRID:Addgene_84458) |
| 参考文献部分: | Fluorescent reporters for markerless genomic integration in Staphylococcus aureus. de Jong NW, van der Horst T, van Strijp JA, Nijland R. Sci Rep. 2017 Mar 7;7:43889. doi: 10.1038/srep43889. 10.1038/srep43889 PubMed 28266573 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pTH100 9969 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION pJB38-NWMN29-30 + SarA_P1-sGFP-Term.
ACCESSION .
VERSION .
KEYWORDS pTH100
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9969)
AUTHORS de Jong NW, van der Horst T, van Strijp JA, Nijland R
TITLE Fluorescent reporters for markerless genomic integration in
Staphylococcus aureus.
JOURNAL Sci Rep. 2017 Mar 7;7:43889. doi: 10.1038/srep43889.
PUBMED 28266573
REFERENCE 2 (bases 1 to 9969)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9969
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 1324..2037
/codon_start=1
/product="GFP variant that folds robustly even when fused
to poorly folded proteins (Pedelacq et al., 2006)"
/label=superfolder GFP
/note="mammalian codon-optimized"
/translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
FVTAAGITHGMDELYK"
primer_bind complement(1354..1382)
/label=GFP-R
/note="GFP, reverse primer. Does NOT anneal to EGFP"
primer_bind 1977..1997
/label=GFP-F
/note="GFP, forward primer. Does NOT anneal to EGFP"
CDS 2044..2061
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(2079..2095)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2079..2095)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
terminator 2104..2138
/label=lambda t0 terminator
/note="minimal transcription terminator from phage lambda
(Scholtissek and Grosse, 1987)"
protein_bind 4965..4983
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O1 for the tetR and tetA genes"
protein_bind 5054..5072
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O1 for the tetR and tetA genes"
CDS 5097..5720
/codon_start=1
/gene="tetR from transposon Tn10"
/product="tetracycline repressor TetR"
/label=TetR
/note="TetR binds to the tetracycline operator tetO to
inhibit transcription. This inhibition can be relieved by
adding tetracycline or doxycycline."
/translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
primer_bind complement(5170..5189)
/label=Tet-R
/note="Tetracycline resistance gene, reverse primer"
primer_bind complement(7711..7733)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 7819..7959
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 7874..7893
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(7974..7991)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8145..8733)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8225..8244)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8904..9764)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9527..9546
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9765..9869)
/gene="bla"
/label=AmpR promoter
primer_bind 9937..9955
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 gaattcagtg gctacattcg aacatatcaa agcatacgat agaaaaacaa taagttatga
61 tatgttacat gtaacaccac ctatgggtcc cttagatgta gtaaaagaaa gtacactttc
121 agatagtgag ggttgggtag atgttaaccc aaccacatta cagcataaaa gctactctaa
181 tgtatttgca cttggtgatg cttcaaatgt acctacttca aaaacaggcg cagctattcg
241 taagcaagca cctatcgtcg ctaataattt attgcaagtg atgaataatc aaatgttaac
301 gcatcattat gatggttata cttcatgccc tattgttact ggatataata ggttaatact
361 tgcagagttt gattataata aaaatactaa agaaacaatg ccgtttaatc aggccaaaga
421 acgtagaagt atgtatatat ttaagaaaga tttattacct aaaatgtatt ggtacggcat
481 gctaaaagga ttaatataat aaagtacaga aaacaataaa tttttaatga aaaatctttt
541 actataaaag attaagtatt taaatgacgt gtcagtgttg tgtttatatg tcgtgaattt
601 ttagctctaa atagtataag attgaaaaag ttgttactgt tttaaatgat cacgatgaag
661 tcattcaata agaatgatta tgaaaataga aacagcagta agatattttc taattgaaaa
721 tcatctcact gctgtttttt aaaggtttat acctcatcct ctaaattatt taaaaataat
781 taatggtatt tgagcacgtt tagcgacttt atgactgaca ttaccaattt ccatttcttg
841 ccagatattc aaaccacgtg tactcaaaat gatagcttgg tatgtacctc caatagtaat
901 ttcaataact ttgtctgttg aacactaaga gcaattttaa tttcataatg tgttgtaaac
961 attttttttg attggagttt ttttctgagt taaacgatat atcgcgattg catgcctgca
1021 ggtcgactct agaggatccg ctagcctgat atttttgact aaaccaaatg ctaacccaga
1081 aatacaatca ctgtgtctaa tgaataattt gttttataaa cacttttttg tttacttctc
1141 atttttaatt agttataatt aactaaataa tagagcatta aatatattta ataaaactta
1201 tttaatgcaa aattatgact aacatatcta taataaataa agattagata tcaatatatt
1261 atcgggcaaa tgtatcgagc aagatgcatc ggatccccgg gtaccttagg aggatgatta
1321 tttatgagca aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
1381 ggtgatgtta atgggcacaa attttctgtc cgtggagagg gtgaaggtga tgctacaaac
1441 ggaaaactca cccttaaatt tatttgcact actggaaaac tacctgttcc gtggccaaca
1501 cttgtcacta ctctgaccta tggtgttcaa tgcttttccc gttatccgga tcacatgaaa
1561 cgtcatgact ttttcaagag tgccatgcct gaaggttatg tacaggaacg cactatatct
1621 ttcaaagatg acgggaccta caagacgcgt gctgaagtca agtttgaagg tgataccctt
1681 gttaatcgta tcgagttaaa gggtattgat tttaaagaag atggaaacat tcttggacac
1741 aaactcgagt acaactttaa ctcacacaat gtatacatca cggcagacaa acaaaagaat
1801 ggaatcaaag ctaacttcaa aattcgccac aacgttgaag atggttccgt tcaactagca
1861 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat
1921 tacctgtcga cacaatctgt cctttcgaaa gatcctaacg aaaagcgtga ccacatggtc
1981 cttcttgagt ttgtaactgc tgctgggatt acacatggca tggatgagct ctacaaagga
2041 tcccaccacc accaccacca ctaagaattc gtaatcatgt catagctgtt tcctgtgtgt
2101 taattcagaa cgctcggttg ccgccgggcg ttttttatgc agctcgcgat atatcctgat
2161 gtatttttaa ttttgcacca tttccaaaag gataagtgac ataagtaaaa aggcatcatc
2221 gggagttatc ctatcaggaa aaccaagata atacctaagt agaaagtgtt caatccgtgt
2281 taaattggga aatatcatcc ataaacttta ttactcatac tataattcaa ttttaacgtc
2341 ttcgtccatt tgggcttcaa attcatcgag tagtgctcgt gcttctgcaa ttgattgtgt
2401 gttcatcaat tgatgtcgaa gttcgctagc gcctcttatg ccacgcacat agattttaaa
2461 gaatctacgc aagctcttga attgtcgtat ttcatctttt tcatatttgt taaacaatga
2521 taaatgcaat ctcaatagat ctaatagttc cttgcttgtg tgttcgcgtg gttctttttc
2581 aaaagcgaat ggattgtgga aaatgcctct accaatcatg acgccatcaa tgccatattt
2641 ttctgccagt tcaagtcctg tttttctatc gggaatatca ccgttaattg ttaacaatgt
2701 atttggtgca atttcgtcac gtaaattttt aatagcttcg attaattccc aatgtgcatc
2761 tactttactc atttctttac gtgtacgaag atgaatagat aaattggcaa tgtcttgttc
2821 gaagacgtgc ttcaaccaat ctttccattc atcgatttca tagtagccaa ggcgtgtttt
2881 aacacttacc ggaagcccac ctgctttagt cgcttgaata atttcggcag caacgtcagg
2941 tcttaagatt aagccggaac ccttacggta cccggggatc ctctagagtc gacctgcagg
3001 catgcaagct ctgatttcac tttttgcatt ctacaaactg cataactcat atgtaaatcg
3061 ctccttttta ggtggcacaa atgtgaggca ttttcgctct ttccggcgag gctagttacc
3121 cttaagttat tggtatgact ggttttaagc gcaaaaaaag ttgctttttc gtacctatta
3181 atgtatcgtt agaaaaccga ctgtaaaaag tacagtcggc attatctcat attataaaag
3241 ccagtcatta ggcctatctg acaattcctg aatagagttc ataaacaatc ctgcatgata
3301 accatcacaa acagaatgat gtacctgtaa agatagcggt aaatatattg aattaccttt
3361 attaatgaat tttcctgctg taataatggg tagaaggtaa ttactattat tattgatatt
3421 taagttaaac ccagtaaatg aagtccatgg aataatagaa agagaaaaag cattttcagg
3481 tataggtgtt ttgggaaaca atttccccga accattatat ttctctacat cagaaaggta
3541 taaatcataa aactctttga agtcattctt tacaggagtc caaataccag agaatgtttt
3601 agatacacca tcaaaaattg tataaagtgg ctctaactta tcccaataac ctaactctcc
3661 gtcgctattg taaccagttc taaaagctgt atttgagttt atcacccttg tcactaagaa
3721 aataaatgca gggtaaaatt tatatccttc ttgttttatg tttcggtata aaacactaat
3781 atcaatttct gtggttatac taaaagtcgt ttgttggttc aaataatgat taaatatctc
3841 ttttctcttc caattgtcta aatcaatttt attaaagttc atttgatatg cctcctaaat
3901 ttttatctaa agtgaattta ggaggcttac ttgtctgctt tcttcattag aatcaatcct
3961 tttttaaaag tcaatattac tgtaacataa atatatattt taaaaatatc ccactttatc
4021 caattttcgt ttgttgaact aatgggtgct ttagttgaag aataaagacc acattaaaaa
4081 atgtggtctt ttgtgttttt ttaaaggatt tgagcgtagc gaaaaatcct tttctttctt
4141 atcttgataa taagggtaac tattgccggg atagactgta acattctcac gcataaaatc
4201 ccctttcatt ttctaatgta aatctattac cttattatta attcaattcg ctcataatta
4261 atcctttttc ttattacgca aaatggcccg atttaagcac accctttatt ccgttaatgc
4321 gccatgacag ccatgataat tactaatact aggagaagtt aataaatacg ggtgatctaa
4381 tgattcaatg attcaaaccc ttgtgaactt ctttagaaca aaagaggttc gtaacaagat
4441 tttcttcaca ctagcaatgt tagtaatttt taaaataggg acttatatac cagctccagg
4501 agtaaatcct gcagcttttg ataatcccca aggttctcaa ggtgccactg agttattaaa
4561 tacttttggt ggcggagcct tgaaacgatt ttctattttt gcaatgggta ttgtacccta
4621 catcactgca tcaatcgtaa tgcaattatt acaaatggat attgtcccta aattctcaga
4681 atgggcaaaa caaggtgaag taggtagaag aaagttaaat aacgttactc gttatttagc
4741 aatttcttta gcatttatcc aatctatagg tatggcattc caatttaata attatctcaa
4801 aggtgcgctg attatcaatc agtcaattat gagttattta ttaatagcac tagttttgac
4861 agcaggaact gctttcttaa tatggcttgg tgatcaaatc actcagttcg gtgttggtaa
4921 tggtatttct attatcatat tcccatcaag cttattttaa ttatactcta tcaatgatag
4981 agtgtcaata ttttttttag tttttcatga actcgatcga ggggatccaa ataaaaaact
5041 agtttgacaa ataactctat caatgataga gtgtcaacaa aaaggaggaa ttaatgatgt
5101 ctagattaga taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa
5161 tcgaaggttt aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt
5221 attggcatgt aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata
5281 ggcaccatac tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata
5341 acgctaaaag ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag
5401 gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc
5461 aacaaggttt ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt
5521 taggttgcgt attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta
5581 ctactgatag tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg
5641 cagagccagc cttcttattc ggccttgaat tgatcatatg cggattagaa aaacaactta
5701 aatgtgaaag tgggtcttaa aagcagcata acctttttcc gtgatggtaa cttcacggta
5761 accaagataa caaagaatac aagaaaatat ttacaaaaaa tcaatttaac aattccttaa
5821 aacatgcagg aattgacgat ttaaacaata ttagctttga acaattctta tctcttttca
5881 atagctataa attatttaat aagtaagtta agggatgcat aaactgcatc ccttaacttg
5941 tttttcgtgt gcctattttt tgtgaatcga ttatgtcttt tgcgcagtcg gcttaaacca
6001 gttttcgctg gtgcgaaaaa agagtgtctt gtgacaccta aattcaaaat ctatcggtca
6061 gatttatacc gatttgattt tatatattct tgaataacat acgccgagtt atcacataaa
6121 agcgggaacc aatcatcaaa tttaaacttc attgcataat ccattaaact cttaaattct
6181 acgattcctt gttcatcaat aaactcaatc atttctttaa ttaatttata tctatctgtt
6241 gttgttttct ttaataattc atcaacatct acaccgccat aaactatcat atcttctttt
6301 tgatatttaa atttattagg atcgtccatg tgaagcatat atctcacaag acctttcaca
6361 cttcctgcaa tctgcggaat agtcgcattc aattcttctg taattatttt tatctgttca
6421 taagatttat taccctcata catcactaga atatgataat gctctttttt catcctatct
6481 tctgtatcag tatccctatc atgtaatgga gacactacaa attgaatgtg taactctttt
6541 aaatactcta accactcggc ttttgctgat tctggatata aaacaaatgt ccaattacgt
6601 cctcttgaat ttttcttgtt ttcagtttct tttattacat tttcgctcat gatataataa
6661 cggtgctaat acatttaaca aaatttagtc atagataggc agcatgccag tgctgtctat
6721 ctttttttgt ttaaaatgca ccgtattcct cctttgcata tttttttatt agaataccgg
6781 ttgcatctga tttgctaata ttatattttt ctttgattct atttaatatc tcattttctt
6841 ctgttgtaag tcttaaagta acagcaactt ttttctcttc ttttctatct acaaccatca
6901 ctgtacctcc caacatctgt ttttttcact ttaacataaa aaacaacctt ttaacattaa
6961 aaacccaata tttatttatt tgtttggaca atggacaatg gacacctagg ggggaggtcg
7021 tagtaccccc ctatgttttc tcccctaaat aaccccaaaa atctaagaaa aaaagacctc
7081 aaaaaggtct ttaattaaca tctcaaattt cgcatttatt ccaatttcct ttttgcgtgt
7141 gatgcgctgc gtccattaaa aatcctagag ctttgcaacc gaaagttaat agctgtcgct
7201 actactttcg cttacgctct aagtatattt taaggactgt cacacgcaaa aagttttctc
7261 ggcataaaag tacctctaca tctctaaatc gtctgtacgc tgtttctcac gctttctatc
7321 gaccttctgg acattatcct gtacaacatc cataaactgt cccacacgct cgaatttgga
7381 atcattaaag aatttctctt taagcctatt aaaccctttc tcaaacccag ggaaattcgc
7441 cctcgcagca cgatataaag tcactgtact atcttgaaat ttctctgata cattcaactg
7501 ctcattcaaa ctatcattct ctcgctttaa tttattaacc tctttacttt tttcgtgata
7561 cccctctttc catgtattca ctacttcttt caaactctct ctacgttttt ttaattcttg
7621 attttctgtg taatagtctg tgctcttaat attttcgtaa tcatcaacaa tccgttcctg
7681 cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
7741 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
7801 tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac
7861 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
7921 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc
7981 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
8041 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
8101 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
8161 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
8221 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
8281 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
8341 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
8401 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
8461 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
8521 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
8581 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
8641 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
8701 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
8761 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
8821 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
8881 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
8941 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
9001 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
9061 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
9121 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
9181 tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc
9241 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
9301 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
9361 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
9421 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
9481 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca
9541 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
9601 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
9661 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
9721 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
9781 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
9841 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
9901 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
9961 cgtcttcaa
//
