| 质粒名称: | pBABEpuro-ERBB2 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 40978 |
| 存储实验室: | Matthew Meyerson |
|
载体骨架基本信息 |
|
| 载体名称: | pBABE-puro |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5200 bp |
| 完整质粒大小: | 9000 bp |
| 载体修饰: | 对pBABE-puro载体进行了修改，使其与网关克隆兼容 |
| 载体类型: | 哺乳动物表达，逆转录病毒 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | ERBB2,HER2 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 3768 bp |
| GenBank编号 | M11730.1 |
| 克隆方法: | Gateway克隆 |
| 5' 测序引物 | tttgtacaccctaagcctc |
| 3' 测序引物 | gcatacttctgcctgctg |
|
文献引用方法 |
|
| 材料和方法部分: | pBABEpuro-ERBB2 was a gift from Matthew Meyerson (Addgene plasmid # 40978 ; http://n2t.net/addgene:40978 ; RRID:Addgene_40978) |
| 参考文献部分: | Functional analysis of receptor tyrosine kinase mutations in lung cancer identifies oncogenic extracellular domain mutations of ERBB2. Greulich H, Kaplan B, Mertins P, Chen TH, Tanaka KE, Yun CH, Zhang X, Lee SH, Cho J, Ambrogio L, Liao R, Imielinski M, Banerji S, Berger AH, Lawrence MS, Zhang J, Pho NH, Walker SR, Winckler W, Getz G, Frank D, Hahn WC, Eck MJ, Mani DR, Jaffe JD, Carr SA, Wong KK, Meyerson M. Proc Natl Acad Sci U S A. 2012 Sep 4;109(36):14476-81. Epub 2012 Aug 20. 10.1073/pnas.1203201109 PubMed 22908275 |
买家导航
pBABEpuroERBB2载体基本信息
pBABEpuroERBB2载体图谱质粒图谱和多克隆位点信息
pBABEpuroERBB2载体简介
pBABEpuroERBB2载体序列
LOCUS pBABEpuro-ERBB2 8907 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pBABEpuro-ERBB2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8907)
AUTHORS Greulich H, Kaplan B, Mertins P, Chen TH, Tanaka KE, Yun CH, Zhang
X, Lee SH, Cho J, Ambrogio L, Liao R, Imielinski M, Banerji S,
Berger AH, Lawrence MS, Zhang J, Pho NH, Walker SR, Winckler W, Getz
G, Frank D, Hahn WC, Eck MJ, Mani DR, Jaffe JD, Carr SA, Wong KK,
Meyerson M
TITLE Functional analysis of receptor tyrosine kinase mutations in lung
cancer identifies oncogenic extracellular domain mutations of ERBB2.
JOURNAL Proc Natl Acad Sci U S A. 2012 Sep 4;109(36):14476-81. Epub 2012 Aug
20.
PUBMED 22908275
REFERENCE 2 (bases 1 to 8907)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8907
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 8..477
/note="long terminal repeat from Moloney murine leukemia
virus"
misc_feature 542..741
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 942..1358
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1288..1310
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1326..1342
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
protein_bind 1386..1410
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
protein_bind complement(5176..5200)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
primer_bind complement(5237..5257)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 5242..5571
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 5422..5557
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5484..5503
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 5581..6180
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(5581..5600)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 6077..6097
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 6401..6870
/note="long terminal repeat from Moloney murine leukemia
virus"
primer_bind complement(7000..7017)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7171..7759)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7251..7270)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7919..8779)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8542..8561
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8780..8884)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
901 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
961 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
1021 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
1081 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
1141 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
1201 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
1261 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
1321 cctcccttta tccagccctc actccttctc taggcgccgg ccggatccca gtgtggtggt
1381 acatcacaag tttgtacaaa aaagttggca tggagctggc ggccttgtgc cgctgggggc
1441 tcctcctcgc cctcttgccc cccggagccg cgagcaccca agtgtgcacc ggcacagaca
1501 tgaagctgcg gctccctgcc agtcccgaga cccacctgga catgctccgc cacctctacc
1561 agggctgcca ggtggtgcag ggaaacctgg aactcaccta cctgcccacc aatgccagcc
1621 tgtccttcct gcaggatatc caggaggtgc agggctacgt gctcatcgct cacaaccaag
1681 tgaggcaggt cccactgcag aggctgcgga ttgtgcgagg cacccagctc tttgaggaca
1741 actatgccct ggccgtgcta gacaatggag acccgctgaa caataccacc cctgtcacag
1801 gggcctcccc aggaggcctg cgggagctgc agcttcgaag cctcacagag atcttgaaag
1861 gaggggtctt gatccagcgg aacccccagc tctgctacca ggacacgatt ttgtggaagg
1921 acatcttcca caagaacaac cagctggctc tcacactgat agacaccaac cgctctcggg
1981 cctgccaccc ctgttctccg atgtgtaagg gctcccgctg ctggggagag agttctgagg
2041 attgtcagag cctgacgcgc actgtctgtg ccggtggctg tgcccgctgc aaggggccac
2101 tgcccactga ctgctgccat gagcagtgtg ctgccggctg cacgggcccc aagcactctg
2161 actgcctggc ctgcctccac ttcaaccaca gtggcatctg tgagctgcac tgcccagccc
2221 tggtcaccta caacacagac acgtttgagt ccatgcccaa tcccgagggc cggtatacat
2281 tcggcgccag ctgtgtgact gcctgtccct acaactacct ttctacggac gtgggatcct
2341 gcaccctcgt ctgccccctg cacaaccaag aggtgacagc agaggatgga acacagcggt
2401 gtgagaagtg cagcaagccc tgtgcccgag tgtgctatgg tctgggcatg gagcacttgc
2461 gagaggtgag ggcagttacc agtgccaata tccaggagtt tgctggctgc aagaagatct
2521 ttgggagcct ggcatttctg ccggagagct ttgatgggga cccagcctcc aacactgccc
2581 cgctccagcc agagcagctc caagtgtttg agactctgga agagatcaca ggttacctat
2641 acatctcagc atggccggac agcctgcctg acctcagcgt cttccagaac ctgcaagtaa
2701 tccggggacg aattctgcac aatggcgcct actcgctgac cctgcaaggg ctgggcatca
2761 gctggctggg gctgcgctca ctgagggaac tgggcagtgg actggccctc atccaccata
2821 acacccacct ctgcttcgtg cacacggtgc cctgggacca gctctttcgg aacccgcacc
2881 aagctctgct ccacactgcc aaccggccag aggacgagtg tgtgggcgag ggcctggcct
2941 gccaccagct gtgcgcccga gggcactgct ggggtccagg gcccacccag tgtgtcaact
3001 gcagccagtt ccttcggggc caggagtgcg tggaggaatg ccgagtactg caggggctcc
3061 ccagggagta tgtgaatgcc aggcactgtt tgccgtgcca ccctgagtgt cagccccaga
3121 atggctcagt gacctgtttt ggaccggagg ctgaccagtg tgtggcctgt gcccactata
3181 aggaccctcc cttctgcgtg gcccgctgcc ccagcggtgt gaaacctgac ctctcctaca
3241 tgcccatctg gaagtttcca gatgaggagg gcgcatgcca gccttgcccc atcaactgca
3301 cccactcctg tgtggacctg gatgacaagg gctgccccgc cgagcagaga gccagccctc
3361 tgacgtccat catctctgcg gtggttggca ttctgctggt cgtggtcttg ggggtggtct
3421 ttgggatcct catcaagcga cggcagcaga agatccggaa gtacacgatg cggagactgc
3481 tgcaggaaac ggagctggtg gagccgctga cacctagcgg agcgatgccc aaccaggcgc
3541 agatgcggat cctgaaagag acggagctga ggaaggtgaa ggtgcttgga tctggcgctt
3601 ttggcacagt ctacaagggc atctggatcc ctgatgggga gaatgtgaaa attccagtgg
3661 ccatcaaagt gttgagggaa aacacatccc ccaaagccaa caaagaaatc ttagacgaag
3721 catacgtgat ggctggtgtg ggctccccat atgtctcccg ccttctgggc atctgcctga
3781 catccacggt gcagctggtg acacagctta tgccctatgg ctgcctctta gaccatgtcc
3841 gggaaaaccg cggacgcctg ggctcccagg acctgctgaa ctggtgtatg cagattgcca
3901 aggggatgag ctacctggag gatgtgcggc tcgtacacag ggacttggcc gctcggaacg
3961 tgctggtcaa gagtcccaac catgtcaaaa ttacagactt cgggctggct cggctgctgg
4021 acattgacga gacagagtac catgcagatg ggggcaaggt gcccatcaag tggatggcgc
4081 tggagtccat tctccgccgg cggttcaccc accagagtga tgtgtggagt tatggtgtga
4141 ctgtgtggga gctgatgact tttggggcca aaccttacga tgggatccca gcccgggaga
4201 tccctgacct gctggaaaag ggggagcggc tgccccagcc ccccatctgc accattgatg
4261 tctacatgat catggtcaaa tgttggatga ttgactctga atgtcggcca agattccggg
4321 agttggtgtc tgaattctcc cgcatggcca gggaccccca gcgctttgtg gtcatccaga
4381 atgaggactt gggcccagcc agtcccttgg acagcacctt ctaccgctca ctgctggagg
4441 acgatgacat gggggacctg gtggatgctg aggagtatct ggtaccccag cagggcttct
4501 tctgtccaga ccctgccccg ggcgctgggg gcatggtcca ccacaggcac cgcagctcat
4561 ctaccaggag tggcggtggg gacctgacac tagggctgga gccctctgaa gaggaggccc
4621 ccaggtctcc actggcaccc tccgaagggg ctggctccga tgtatttgat ggtgacctgg
4681 gaatgggggc agccaagggg ctgcaaagcc tccccacaca tgaccccagc cctctacagc
4741 ggtacagtga ggaccccaca gtacccctgc cctctgagac tgatggctac gttgcccccc
4801 tgacctgcag cccccagcct gaatatgtga accagccaga tgttcggccc cagccccctt
4861 cgccccgaga gggccctctg cctgctgccc gacctgctgg tgccactctg gaaaggccca
4921 agactctctc cccagggaag aatggggtcg tcaaagacgt ttttgccttt gggggtgccg
4981 tggagaaccc cgagtacttg acaccccagg gaggagctgc ccctcagccc caccctcctc
5041 ctgccttcag cccagccttc gacaacctct attactggga ccaggaccca ccagagcggg
5101 gggctccacc cagcaccttc aaagggacac ctacggcaga gaacccagag tacctgggtc
5161 tggacgtgcc agtgtgacca actttcttgt acaaagtggt gatgtaggaa ttcgccagca
5221 cagtggtcga ccctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
5281 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
5341 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
5401 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
5461 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
5521 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttacc
5581 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
5641 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
5701 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
5761 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
5821 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
5881 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
5941 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
6001 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
6061 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
6121 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
6181 cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca tgcatcgata
6241 aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg
6301 tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaaatac ataactgaga
6361 atagagaagt tcagatcaag gtcaggaaca gatggaacag ctgaatatgg gccaaacagg
6421 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg aacagctgaa
6481 tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca
6541 gatggtcccc agatgcggtc cagccctcag cagtttctag agaaccatca gatgtttcca
6601 gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct
6661 tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc
6721 actcggggcg ccagtcctcc gattgactga gtcgcccggg tacccgtgta tccaataaac
6781 cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt
6841 gattgactac ccgtcagcgg gggtctttca catgcagcat gtatcaaaat taatttggtt
6901 ttttttctta agtatttaca ttaaatggcc atagttgcat taatgaatcg gccaacgcgc
6961 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
7021 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
7081 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
7141 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
7201 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
7261 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
7321 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
7381 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
7441 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
7501 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
7561 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
7621 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
7681 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
7741 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
7801 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
7861 gatccttttg cggccgcaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
7921 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
7981 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
8041 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
8101 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
8161 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
8221 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
8281 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
8341 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
8401 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
8461 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
8521 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
8581 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
8641 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
8701 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
8761 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
8821 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
8881 cgcgcacatt tccccgaaaa gtgccac
//
