| 质粒名称: | pSH-EFIRES-B-AtAFB2-mCherry |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 129718 |
| 存储实验室: | Elina Ikonen |
|
载体骨架基本信息 |
|
| 载体名称: | pSH-EFIRES-B |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 8000 bp |
| 完整质粒大小: | 10442 bp |
| 载体类型: | 哺乳动物表达，CRISPR，TALEN；人类安全港基因座（AVS1）位点 |
| 筛选标记: | 杀螟素；富集FACS的mCherry |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | AFB2,auxin signaling F-box 2 |
| 物种来源: | A. thaliana (mustard weed) |
| 插入大小: | 2445 bp |
| 基因突变: | codon optimized |
| 启动子 | EF1a |
| 标签/融合蛋白: | mCherry（插入件上的C端子） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BglII(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | tctctccacaggtgtccact |
| 3' 测序引物 | acaccggccttattccaagc |
|
文献引用方法 |
|
| 材料和方法部分: | pSH-EFIRES-B-AtAFB2-mCherry was a gift from Elina Ikonen (Addgene plasmid # 129718 ; http://n2t.net/addgene:129718 ; RRID:Addgene_129718) |
| 参考文献部分: | An efficient auxin-inducible degron system with low basal degradation in human cells. Li S, Prasanna X, Salo VT, Vattulainen I, Ikonen E. Nat Methods. 2019 Aug 26. pii: 10.1038/s41592-019-0512-x. doi: 10.1038/s41592-019-0512-x. 10.1038/s41592-019-0512-x PubMed 31451765 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pSH-EFIRES-B-AtAFB2-mCherry 10442 bp ds-DNA circular SYN 22-AUG-2019
DEFINITION AAVS1 safe harbor targeting vector with blasticidin resistance gene
expressing AtAFB2-mCherry.
ACCESSION .
VERSION .
KEYWORDS pSH-EFIRES-B-AtAFB2-mCherry
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10442)
TITLE An efficient auxin-inducible degron system with low basal
degradation in human cells
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 10442)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 22, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10442
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(135..152)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(306..894)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(386..405)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(1065..1925)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1688..1707
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(1926..2030)
/gene="bla"
/label=AmpR promoter
rep_origin 2057..2512
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2144..2163)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 2354..2375
/label=F1ori-F
/note="F1 origin, forward primer"
misc_feature 3029..3832
/label=HA-L
/note="left homology arm from the adeno-associated virus
integration site (AAVS1) within intron 1 of the human
PPP1R12C gene"
polyA_signal 3858..3906
/note="synthetic polyadenylation signal"
misc_feature 3920..4011
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
primer_bind 3960..3979
/label=RVprimer3
/note="pGL3 vector, forward primer"
promoter 4023..5185
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 4254..5176
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 5133..5153
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
intron 5224..5356
/label=chimeric intron
/note="chimera between introns from human beta-globin and
immunoglobulin heavy chain genes"
primer_bind 5339..5358
/label=pMT2-F
/note="Synthetic intron, forward primer"
promoter 5401..5419
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 7186..7893
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
GRHSTGGMDELYK"
primer_bind complement(7328..7346)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 7596..7615
/label=mCherry-F
/note="mCherry, forward primer"
misc_feature 7928..8480
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(8072..8089)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 8299..8318
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 8480..8881
/codon_start=1
/gene="Aspergillus terreus BSD"
/product="blasticidin S deaminase"
/label=BSD
/note="confers resistance to blasticidin"
/translation="MGAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFT
GVNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPG
IKAIVKDSDGQPTAVGIRELLPSGYVWEG"
polyA_signal 9039..9160
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(9076..9095)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 9130..9149
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
misc_feature 9196..10032
/label=HA-R
/note="right homology arm from the adeno-associated virus
integration site (AAVS1) within intron 1 of the human
PPP1R12C gene"
ORIGIN
1 cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta
61 tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag
121 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
181 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
241 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
301 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
361 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
421 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
481 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
541 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
601 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
661 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
721 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
781 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
841 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
901 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
961 ttggtcatga gattatcaaa aaggatattc acctagatcc ttttaaatta aaaatgaagt
1021 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
1081 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
1141 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
1201 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
1261 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
1321 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
1381 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
1441 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
1501 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
1561 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
1621 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
1681 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
1741 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
1801 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
1861 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
1921 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
1981 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
2041 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
2101 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
2161 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
2221 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
2281 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
2341 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
2401 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
2461 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttgccattcg
2521 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
2581 cagcccaagc taccatgata agtaagtaat attaaggtac gggaggtact tggagcggcc
2641 gtcgacctac tctcttccgc attggagtcg ctttaactgg ccctggcttt ggcagcctgt
2701 gctgacccat gcagtcctcc ttaccatccc tccctcgact tcccctcttc cgatgttgag
2761 cccctccagc cggtcctgga ctttgtctcc ttccctgccc tgccctctcc tgaacctgag
2821 ccagctccca tagctcagtc tggtctatct gcctggccct ggccattgtc actttgcgct
2881 gccctcctct cgcccccgag tgcccttgct gtgccgccgg aactctgccc tctaacgctg
2941 ccgtctctct cctgagtccg gaccactttg agctctactg gcttctgcgc cgcctctggc
3001 ccactgtttc cccttcccag gcaggtcctg ctttctctga cctgcattct ctcccctggg
3061 cctgtgccgc tttctgtctg cagcttgtgg cctgggtcac ctctacggct ggcccagatc
3121 cttccctgcc gcctccttca ggttccgtct tcctccactc cctcttcccc ttgctctctg
3181 ctgtgttgct gcccaaggat gctctttccg gagcacttcc ttctcggcgc tgcaccacgt
3241 gatgtcctct gagcggatcc tccccgtgtc tgggtcctct ccgggcatct ctcctccctc
3301 acccaacccc atgccgtctt cactcgctgg gttccctttt ccttctcctt ctggggcctg
3361 tgccatctct cgtttcttag gatggccttc tccgacggat gtctcccttg cgtcccgcct
3421 ccccttcttg taggcctgca tcatcaccgt ttttctggac aaccccaaag taccccgtct
3481 ccctggcttt agccacctct ccatcctctt gctttctttg cctggacacc ccgttctcct
3541 gtggattcgg gtcacctctc actcctttca tttgggcagc tcccctaccc cccttacctc
3601 tctagtctgt gctagctctt ccagccccct gtcatggcat cttccagggg tccgagagct
3661 cagctagtct tcttcctcca acccgggccc ctatgtccac ttcaggacag catgtttgct
3721 gcctccaggg atcctgtgtc cccgagctgg gaccacctta tattcccagg gccggttaat
3781 gtggctctgg ttctgggtac ttttatctgt cccctccacc ccacagtggg gcgcatgcgt
3841 ggaattatca cctcgagaat aaaatatctt tattttcatt acatctgtgt gttggttttt
3901 tgtgtgaatc gatagtacta acatacgctc tccatcaaaa caaaacgaaa caaaacaaac
3961 tagcaaaata ggctgtcccc agtgcaagtg caggtgccag aacatttctc tactagtcgt
4021 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
4081 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
4141 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
4201 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
4261 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
4321 ttacttccac ctggctccag tacgtgattc ttgatcccga gctggagcca ggggcgggcc
4381 ttgcgcttta ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
4441 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
4501 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
4561 aaatgcgggc caggatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
4621 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
4681 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
4741 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
4801 aagatggccg cttcccggcc ctgctccagg gggctcaaaa tggaggacgc ggcgctcggg
4861 agagcgggcg ggtgagtcac ccacacaaag gaaaggggcc tttccgtcct cagccgtcgc
4921 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctggagctt
4981 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
5041 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
5101 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
5161 ttttcttcca tttcaggtgt cgtgaacacg ggctgcagaa gttggtcgtg aggcactggg
5221 caggtaagta tcaaggttac aagacaggtt taaggagacc aatagaaact gggcttgtcg
5281 agacagagaa gactcttgcg tttctgatag gcacctattg gtcttactga catccacttt
5341 gcctttctct ccacaggtgt ccactcccag ttcaattaca gctcttaagg ctagagtact
5401 taatacgact cactataggc tagcgctacc ggactcagat ctaccatgaa ctactttccc
5461 gatgaggtca ttgagcacgt ctttgatttc gtcacaagcc acaaggatag gaacgccatt
5521 agcctggtct gtaagagctg gtacaagatc gagcggtatt ccagacagaa ggtgttcatc
5581 ggcaactgct acgccatcaa tccagagagg ctgctgcgga gatttccctg tctgaagagc
5641 ctgaccctga agggcaagcc ccacttcgcc gactttaacc tggtgcctca cgagtgggga
5701 ggattcgtgc tgccatggat cgaggccctg gcacggtcca gagtgggcct ggaggagctg
5761 aggctgaagc gcatggtggt gacagacgag tctctggagc tgctgtctcg gagcttcgtg
5821 aacttcaaga gcctggtgct ggtgtcctgc gagggcttta ccacagatgg cctggcaagc
5881 atcgcagcaa actgtaggca cctgcgggac ctggacctgc aggagaatga gatcgacgat
5941 cacagaggcc agtggctgtc ctgcttcccc gatacctgta ccacactggt gacactgaac
6001 tttgcctgcc tggagggcga gacaaatctg gtggccctgg agaggctggt ggcacgctct
6061 cccaacctga agagcctgaa gctgaatagg gcagtgcctc tggacgcact ggcaagactg
6121 atggcatgcg caccacagat cgtggatctg ggcgtgggct cctacgagaa cgaccctgat
6181 tccgagtctt atctgaagct gatggccgtg atcaagaagt gtaccagcct gcgcagcctg
6241 tccggcttcc tggaggcagc acctcactgc ctgtccgcct ttcacccaat ctgtcacaac
6301 ctgacatccc tgaatctgtc ttacgccgcc gagatccacg gctcccacct gatcaagctg
6361 atccagcact gcaagaagct gcagaggctg tggatcctgg actccatcgg cgataagggc
6421 ctggaggtgg tggcctctac ctgtaaggag ctgcaggagc tgcgcgtgtt cccatctgac
6481 ctgctgggag gaggaaacac cgcagtgaca gaggagggcc tggtggcaat ctctgccgga
6541 tgccccaagc tgcacagcat cctgtatttt tgtcagcaga tgaccaacgc cgccctggtg
6601 acagtggcca agaactgccc caatttcatc aggtttcgcc tgtgcatcct ggagcccaat
6661 aagcctgacc acgtgacatc tcagcctctg gatgagggct tcggcgccat cgtgaaggcc
6721 tgcaagagcc tgaggcgcct gtctctgagc ggcctgctga ccgaccaggt gttcctgtac
6781 atcggcatgt atgccaacca gctggagatg ctgtctatcg cctttgccgg cgacacagat
6841 aagggcatgc tgtacgtgct gaatggctgt aagaagatga agaagctgga gatcagagac
6901 agcccttttg gcgataccgc cctgctggca gacgtgagca agtatgagac aatgcggagc
6961 ctgtggatga gctcctgcga ggtgaccctg agcggctgta agagactggc cgagaaggcc
7021 ccatggctga acgtggagat catcaacgag aatgacaaca ataggatgga ggagaatggc
7081 cacgagggcc gccagaaggt ggataagctg tatctgtata ggaccgtcgt cgggactcgg
7141 atggatgccc ccccctttgt ctggattctg ggcggatctg gcggggtgag caagggcgag
7201 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
7261 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
7321 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
7381 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
7441 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
7501 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
7561 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
7621 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
7681 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
7741 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
7801 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
7861 tccaccggcg gcatggacga gctgtacaag tagcggccgc tctagcccaa ttccgccccc
7921 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
7981 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
8041 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
8101 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
8161 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
8221 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
8281 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
8341 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
8401 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
8461 tgaaaaacac gatgataata tgggcgccaa gcctttgtct caagaagaat ccaccctcat
8521 tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag
8581 cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg
8641 gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct
8701 gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg
8761 ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg
8821 acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta
8881 agcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg
8941 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
9001 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
9061 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
9121 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
9181 cctctagtat gcatcactag ggacaggatt ggtgacagaa aagccccatc cttaggcctc
9241 ctccttccta gtctcctgat attgggtcta acccccacct cctgttaggc agattcctta
9301 tctggtgaca cacccccatt tcctggagcc atctctctcc ttgccagaac ctctaaggtt
9361 tgcttacgat ggagccagag aggatcctgg gagggagagc ttggcagggg gtgggaggga
9421 agggggggat gcgtgacctg cccggttctc agtggccacc ctgcgctacc ctctcccaga
9481 acctgagctg ctctgacgcg gctgtctggt gcgtttcact gatcctggtg ctgcagcttc
9541 cttacacttc ccaagaggag aagcagtttg gaaaaacaaa atcagaataa gttggtcctg
9601 agttctaact ttggctcttc acctttctag tccccaattt atattgttcc tccgtgcgtc
9661 agttttacct gtgagataag gccagtagcc agccccgtcc tggcagggct gtggtgagga
9721 ggggggtgtc cgtgtggaaa actccctttg tgagaatggt gcgtcctagg tgttcaccag
9781 gtcgtggccg cctctactcc ctttctcttt ctccatcctt ctttccttaa agagtcccca
9841 gtgctatctg ggacatattc ctccgcccag agcagggtcc cgcttcccta aggccctgct
9901 ctgggcttct gggtttgagt ccttggcaag cccaggagag gcgctcaggc ttccctgtcc
9961 cccttcctcg tccaccatct catgcccctg gctctcctgc cccttcccta caggggttcc
10021 tggctctgct cttcagactg agccccgttc ccctgcatcc ccgttcccct gcatccccct
10081 tcccctgcat cccccagagg ccccaggcca cctacttggc ctggacccca cgagaggcca
10141 ccccagccct gtctaccagg ctgccttttg ggtggattct cctccaactg tggggtgact
10201 gcttggcaaa ctcactcttc ggggtatccc aggaggcctg gagcattggg gtgggctggg
10261 gttcagagag gagggattcc cttctcaggt tacgtggcca agaagcaggg gagctgggtt
10321 tgggtcaggt ctgggtgtgg ggtgaccagc ttatgctgtt tgcccaggac agcctagttt
10381 tagcgctgaa accctcagtc ctaggaaaac agggatggtt ggtcactgtc tctgggctcg
10441 ac
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