出品公司: | Agilent |
---|---|
载体名称: | pAdTrack-CMV, pAdTrack CMV, pAdTrackCMV |
质粒类型: | 哺乳动物细胞腺病毒表达载体 |
高拷贝/低拷贝: | 低拷贝 |
启动子: | CMV |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 9220 bp |
5' 测序引物及序列: | / |
3' 测序引物及序列: | / |
载体标签: | EGFP |
载体抗性: | 卡那 |
备注: | pAdTrack-CMV载体用来转基因腺病毒细胞表达,载体拥有CMV启动子和EGFP绿色荧光蛋白跟踪基因表达结果,和pAdeasy1载体共同使用时最大能够插入 5.9kb,pAdeasy2载体共同使用时能够最大插入8.6kb片段。具体使用方法可以参考pAdeasy载体系统。 |
产品目录号: | -- |
稳定性: | / |
组成型: | -- |
病毒/非病毒: | 腺病毒 |
买家导航
pAdTrack-CMV载体 pAdTrackCMV质粒基本信息
pAdTrack-CMV质粒图谱 pAdTrackCMV载体图谱和pAdTrack-CMV载体序列 pAdTrackCMV质粒序列多克隆位点信息
pAdTrack-CMV质粒 pAdTrackCMV载体简介
pAdTrack-CMV质粒序列 pAdTrackCMV载体序列
LOCUS pAdTrack-CMV 9220 bp DNA circular SYN DEFINITION pAdTrack-CMV ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI http://www.biofeng.com/ COMMENT VNTAUTHORNAME|biofeng.com| FEATURES Location/Qualifiers source 1..9044 /organism="pAdTrack-CMV" /mol_type="other DNA" misc_feature 196..344 /label="Encap" misc_feature complement(373..392) /label="EBV_rev_primer" CDS 574..1353 /label="ORF frame 1" CDS complement(592..1338) /label="ORF frame 3" gene complement(622..1335) /label="EGFP" /gene="EGFP" misc_feature complement(664..685) /label="EGFP_C_primer" misc_feature complement(1126..1155) /label="Y66 (EGFP)" misc_feature 1272..1293 /label="EGFP_N_primer" misc_feature complement(1412..1432) /label="CMV_fwd_primer" misc_feature complement(1963..1982) /label="EBV_rev_primer" promoter complement(2332..2403) /label="CMV_promoter" misc_feature complement(2382..2402) /label="CMV_fwd_primer" misc_feature complement(2568..2843) /label="CAG_enhancer" CDS complement(3462..4820) /label="ORF frame 1" CDS complement(4568..5089) /label="ORF frame 2" misc_feature complement(6046..6141) /label="LITR" misc_feature 6046..6141 /label="RITR" rep_origin complement(6386..7005) /label="pBR322_origin" gene complement(7160..7459) /label="Ampicillin (299 - 0)" /gene="Ampicillin (299 - 0)" promoter 7664..7713 /label="NEOKAN_promoter" CDS 7802..8596 /label="ORF frame 2" gene 7805..8593 /label="NeoR/KanR" /gene="NeoR/KanR" ORIGIN 1 NNNTTAATTA ANNNTCCCTT CCAGCTCTCT GCCCCTTTTG GATTGAAGCC AATATGATAA 61 TGAGGGGGTG GAGTTTGTGA CGTGGCGCGG GCGTGGGAAC GGGGCGGGTG ACGTAGTAGT 121 GTGGCGGAAG TGTGATGTTG CAAGTGTGGC GGAACACATG TAAGCGACGG ATGTGGCAAA 181 AGTGACGTTT TTGGTGTGCG CCGGTGTACA CAGGAAGTGA CAATTTTCGC GCGGTTTTAG 241 GCGGATGTTG TAGTAAATTT GGGCGTAACC GAGTAAGATT TGGCCATTTT CGCGGGAAAA 301 CTGAATAAGA GGAAGTGAAA TCTGAATAAT TTTGTGTTAC TCATAGCGCG TAANNNCGCG 361 TTAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA AAAATGCTTT 421 ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG CAATAAACAA 481 GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT 541 TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTATGGCTG ATTATGATCA GTTATCTAGA 601 TCCGGTGGAT CTGAGTCCGG ACTTGTACAG CTCGTCCATG CCGAGAGTGA TCCCGGCGGC 661 GGTCACGAAC TCCAGCAGGA CCATGTGATC GCGCTTCTCG TTGGGGTCTT TGCTCAGGGC 721 GGACTGGGTG CTCAGGTAGT GGTTGTCGGG CAGCAGCACG GGGCCGTCGC CGATGGGGGT 781 GTTCTGCTGG TAGTGGTCGG CGAGCTGCAC GCTGCCGTCC TCGATGTTGT GGCGGATCTT 841 GAAGTTCACC TTGATGCCGT TCTTCTGCTT GTCGGCCATG ATATAGACGT TGTGGCTGTT 901 GTAGTTGTAC TCCAGCTTGT GCCCCAGGAT GTTGCCGTCC TCCTTGAAGT CGATGCCCTT 961 CAGCTCGATG CGGTTCACCA GGGTGTCGCC CTCGAACTTC ACCTCGGCGC GGGTCTTGTA 1021 GTTGCCGTCG TCCTTGAAGA AGATGGTGCG CTCCTGGACG TAGCCTTCGG GCATGGCGGA 1081 CTTGAAGAAG TCGTGCTGCT TCATGTGGTC GGGGTAGCGG CTGAAGCACT GCACGCCGTA 1141 GGTCAGGGTG GTCACGAGGG TGGGCCAGGG CACGGGCAGC TTGCCGGTGG TGCAGATGAA 1201 CTTCAGGGTC AGCTTGCCGT AGGTGGCATC GCCCTCGCCC TCGCCGGACA CGCTGAACTT 1261 GTGGCCGTTT ACGTCGCCGT CCAGCTCGAC CAGGATGGGC ACCACCCCGG TGAACAGCTC 1321 CTCGCCCTTG CTCACCATGG TGGCGACCGG TAGCGCTAGC GGATCTGACG GTTCACTAAA 1381 CCAGCTCTGC TTATATAGAC CTCCCACCGT ACACGCCTAC CGCCCATTTG CGTCAATGGG 1441 GCGGAGTTGT TACGACATTT TGGAAAGTCC CGTTGATTTT GGTGCCAAAA CAAACTCCCA 1501 TTGACGTCAA TGGGGTGGAG ACTTGGAAAT CCCCGTGAGT CAAACCGCTA TCCACGCCCA 1561 TTGATGTACT GCCAAAACCG CATCACCATG GTAATAGCGA TGACTAATAC GTAGATGTAC 1621 TGCCAAGTAG GAAAGTCCCA TAAGGTCATG TACTGGGCAT AATGCCAGGC GGGCCATTTA 1681 CCGTCATTGA CGTCAATAGG GGGCGTACTT GGCATATGAT ACACTTGATG TACTGCCAAG 1741 TGGGCAGTTT ACCGTAAATA CTCCACCCAT TGACGTCAAT GGAAAGTCCC TATTGGCGTT 1801 ACTATGGGAA CATACGTCAT TATTGACGTC AATGGGCGGG GGTCGTTGGG CGGTCAGCCA 1861 GGCGGGCCAT TTACCGTAAG TTATGTAACG CGGAACTCCA TATATGGGCT ATGAACTAAT 1921 GACCCCGTAA TTGATTACTA TTANNNCGCG TTAAGATACA TTGATGAGTT TGGACAAACC 1981 ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA 2041 TTTGTAACCA TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG 2101 TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT 2161 GGTATGGCTG ATTATGATCA GTTATCTAGA TCCGGTGGAT CTGAGTCCGG ACTTGTACAG 2221 CTCGTCCATG CCGAGAGTGA TCCCGGCGGC GGTCACGAAC TCCANNGATC GGATATCTTA 2281 TCTAGAAGCT TAGGCTCGAG CGGCCGCGTC GACGGTACCA GATCTCTAGC GGATCTGACG 2341 GTTCACTAAA CCAGCTCTGC TTATATAGAC CTCCCACCGT ACACGCCTAC CGCCCATTTG 2401 CGTCAATGGG GCGGAGTTGT TACGACATTT TGGAAAGTCC CGTTGATTTT GGTGCCAAAA 2461 CAAACTCCCA TTGACGTCAA TGGGGTGGAG ACTTGGAAAT CCCCGTGAGT CAAACCGCTA 2521 TCCACGCCCA TTGATGTACT GCCAAAACCG CATCACCATG GTAATAGCGA TGACTAATAC 2581 GTAGATGTAC TGCCAAGTAG GAAAGTCCCA TAAGGTCATG TACTGGGCAT AATGCCAGGC 2641 GGGCCATTTA CCGTCATTGA CGTCAATAGG GGGCGTACTT GGCATATGAT ACACTTGATG 2701 TACTGCCAAG TGGGCAGTTT ACCGTAAATA CTCCACCCAT TGACGTCAAT GGAAAGTCCC 2761 TATTGGCGTT ACTATGGGAA CATACGTCAT TATTGACGTC AATGGGCGGG GGTCGTTGGG 2821 CGGTCAGCCA GGCGGGCCAT TTACCAACGC GGAACTCCAT ATATGGGCTA TGAACTAATG 2881 ACCCCGTAAT TGATTACTAT TANNNTAAGG GTGGGAAAGA ATATATAAGG TGGGGGTCTT 2941 ATGTAGTTTT GTATCTGTTT TGCAGCAGCC GCCGCCGCCA TGAGCACCAA CTCGTTTGAT 3001 GGAAGCATTG TGAGCTCATA TTTGACAACG CGCATGCCCC CATGGGCCGG GGTGCGTCAG 3061 AATGTGATGG GCTCCAGCAT TGATGGTCGC CCCGTCCTGC CCGCAAACTC TACTACCTTG 3121 ACCTACGAGA CCGTGTCTGG AACGCCGTTG GAGACTGCAG CCTCCGCCGC CGCTTCAGCC 3181 GCTGCAGCCA CCGCCCGCGG GATTGTGACT GACTTTGCTT TCCTGAGCCC GCTTGCAAGC 3241 AGTGCAGCTT CCCGTTCATC CGCCCGCGAT GACAAGTTGA CGGCTCTTTT GGCACAATTG 3301 GATTCTTTGA CCCGGGAACT TAATGTCGTT TCTCAGCAGC TGTTGGATCT GCGCCAGCAG 3361 GTTTCTGCCC TGAAGGCTTC CTCCCCTCCC AATGCGGTTT AAAACATAAA TAAAAAACCA 3421 GACTCTGTTT GGATTTGGAT CAAGCAAGTG TCTTGCTGTC TTTATTTAGG GGTTTTGCGC 3481 GCGCGGTAGG CCCGGGACCA GCGGTCTCGG TCGTTGAGGG TCCTGTGTAT TTTTTCCAGG 3541 ACGTGGTAAA GGTGACTCTG GATGTTCAGA TACATGGGCA TAAGCCCGTC TCTGGGGTGG 3601 AGGTAGCACC ACTGCAGAGC TTCATGCTGC GGGGTGGTGT TGTAGATGAT CCAGTCGTAG 3661 CAGGAGCGCT GGGCGTGGTG CCTAAAAATG TCTTTCAGTA GCAAGCTGAT TGCCAGGGGC 3721 AGGCCCTTGG TGTAAGTGTT TACAAAGCGG TTAAGCTGGG ATGGGTGCAT ACGTGGGGAT 3781 ATGAGATGCA TCTTGGACTG TATTTTTAGG TTGGCTATGT TCCCAGCCAT ATCCCTCCGG 3841 GGATTCATGT TGTGCAGAAC CACCAGCACA GTGTATCCGG TGCACTTGGG AAATTTGTCA 3901 TGTAGCTTAG AAGGAAATGC GTGGAAGAAC TTGGAGACGC CCTTGTGACC TCCAAGATTT 3961 TCCATGCATT CGTCCATAAT GATGGCAATG GGCCCACGGG CGGCGGCCTG GGCGAAGATA 4021 TTTCTGGGAT CACTAACGTC ATAGTTGTGT TCCAGGATGA GATCGTCATA GGCCATTTTT 4081 ACAAAGCGCG GGCGGAGGGT GCCAGACTGC GGTATAATGG TTCCATCCGG CCCAGGGGCG 4141 TAGTTACCCT CACAGATTTG CATTTCCCAC GCTTTGAGTT CAGATGGGGG GATCATGTCT 4201 ACCTGCGGGG CGATGAAGAA AACGGTTTCC GGGGTAGGGG AGATCAGCTG GGAAGAAAGC 4261 AGGTTCCTGA GCAGCTGCGA CTTACCGCAG CCGGTGGGCC CGTAAATCAC ACCTATTACC 4321 GGGTGCAACT GGTAGTTAAG AGAGCTGCAG CTGCCGTCAT CCCTGAGCAG GGGGGCCACT 4381 TCGTTAAGCA TGTCCCTGAC TCGCATGTTT TCCCTGACCA AATCCGCCAG AAGGCGCTCG 4441 CCGCCCAGCG ATAGCAGTTC TTGCAAGGAA GCAAAGTTTT TCAACGGTTT GAGACCGTCC 4501 GCCGTAGGCA TGCTTTTGAG CGTTTGACCA AGCAGTTCCA GGCGGTCCCA CAGCTCGGTC 4561 ACCTGCTCTA CGGCATCTCG ATCCAGCATA TCTCCTCGTT TCGCGGGTTG GGGCGGCTTT 4621 CGCTGTACGG CAGTAGTCGG TGCTCGTCCA GACGGGCCAG GGTCATGTCT TTCCACGGGC 4681 GCAGGGTCCT CGTCAGCGTA GTCTGGGTCA CGGTGAAGGG GTGCGCTCCG GGCTGCGCGC 4741 TGGCCAGGGT GCGCTTGAGG CTGGTCCTGC TGGTGCTGAA GCGCTGCCGG TCTTCGCCCT 4801 GCGCGTCGGC CAGGTAGCAT TTGACCATGG TGTCATAGTC CAGCCCCTCC GCGGCGTGGC 4861 CCTTGGCGCG CAGCTTGCCC TTGGAGGAGG CGCCGCACGA GGGGCAGTGC AGACTTTTGA 4921 GGGCGTAGAG CTTGGGCGCG AGAAATACCG ATTCCGGGGA GTAGGCATCC GCGCCGCAGG 4981 CCCCGCAGAC GGTCTCGCAT TCCACGAGCC AGGTGAGCTC TGGCCGTTCG GGGTCAAAAA 5041 CCAGGTTTCC CCCATGCTTT TTGATGCGTT TCTTACCTCT GGTTTCCATG AGCCGGTGTC 5101 CACGCTCGGT GACGAAAAGG CTGTCCGTGT CCCCGTATAC AGACTNNNGT TTAAACGAAT 5161 TCNNNTATAA AATGCAAGGT GCTGCTCAAA AAATCAGGCA AAGCCTCGCG CAAAAAAGAA 5221 AGCACATCGT AGTCATGCTC ATGCAGATAA AGGCAGGTAA GCTCCGGAAC CACCACAGAA 5281 AAAGACACCA TTTTTCTCTC AAACATGTCT GCGGGTTTCT GCATAAACAC AAAATAAAAT 5341 AACAAAAAAA CATTTAAACA TTAGAAGCCT GTCTTACAAC AGGAAAAACA ACCCTTATAA 5401 GCATAAGACG GACTACGGCC ATGCCGGCGT GACCGTAAAA AAACTGGTCA CCGTGATTAA 5461 AAAGCACCAC CGACAGCTCC TCGGTCATGT CCGGAGTCAT AATGTAAGAC TCGGTAAACA 5521 CATCAGGTTG ATTCATCGGT CAGTGCTAAA AAGCGACCGA AATAGCCCGG GGGAATACAT 5581 ACCCGCAGGC GTAGAGACAA CATTACAGCC CCCATAGGAG GTATAACAAA ATTAATAGGA 5641 GAGAAAAACA CATAAACACC TGAAAAACCC TCCTGCCTAG GCAAAATAGC ACCCTCCCGC 5701 TCCAGAACAA CATACAGCGC TTCACAGCGG CAGCCTAACA GTCAGCCTTA CCAGTAAAAA 5761 AGAAAACCTA TTAAAAAAAC ACCACTCGAC ACGGCACCAG CTCAATCAGT CACAGTGTAA 5821 AAAAGGGCCA AGTGCAGAGC GAGTATATAT AGGACTAAAA AATGACGTAA CGGTTAAAGT 5881 CCACAAAAAA CACCCAGAAA ACCGCACGCG AACCTACGCC CAGAAACGAA AGCCAAAAAA 5941 CCCACAACTT CCTCAAATCG TCACTTCCGT TTTCCCACGT TACGTAACTT CCCATTTTAA 6001 GAAAACTACA ATTCCCAACA CATACAAGTT ACTCCGCCCT AAAACCTACG TCACCCGCCC 6061 CGTTCCCACG CCCCGCGCCA CGTCACAAAC TCCACCCCCT CATTATCATA TTGGCTTCAA 6121 TCCAAAATAA GGTATATTAT TGATGATNNN TTAATTAAGG ATCCNNNCGG TGTGAAATAC 6181 CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGCTC TTCCGCTTCC TCGCTCACTG 6241 ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA 6301 TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC 6361 AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC 6421 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT 6481 AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC 6541 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT 6601 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG 6661 AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC 6721 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA 6781 GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA 6841 GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA 6901 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC 6961 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG 7021 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA 7081 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG 7141 AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT 7201 GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG 7261 AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC 7321 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA 7381 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC 7441 CAGTTAATAG TTTGCGCAAC GTTGTTGNNN NNNAAAAAGG ATCTTCACCT AGATCCTTTT 7501 CACGTAGAAA GCCAGTCCGC AGAAACGGTG CTGACCCCGG ATGAATGTCA GCTACTGGGC 7561 TATCTGGACA AGGGAAAACG CAAGCGCAAA GAGAAAGCAG GTAGCTTGCA GTGGGCTTAC 7621 ATGGCGATAG CTAGACTGGG CGGTTTTATG GACAGCAAGC GAACCGGAAT TGCCAGCTGG 7681 GGCGCCCTCT GGTAAGGTTG GGAAGCCCTG CAAAGTAAAC TGGATGGCTT TCTCGCCGCC 7741 AAGGATCTGA TGGCGCAGGG GATCAAGCTC TGATCAAGAG ACAGGATGAG GATCGTTTCG 7801 CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT 7861 CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC 7921 AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT 7981 GCAAGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT 8041 GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA 8101 GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT 8161 GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG 8221 CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA 8281 AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGA GCATGCCCGA 8341 CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA 8401 TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA 8461 CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT 8521 CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT 8581 TGACGAGTTC TTCTGAATTT TGTTAAAATT TTTGTTAAAT CAGCTCATTT TTTAACCAAT 8641 AGGCCGAAAT CGGCAACATC CCTTATAAAT CAAAAGAATA GACCGCGATA GGGTTGAGTG 8701 TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT GGACTCCAAC GTCAAAGGGC 8761 GAAAAACCGT CTATCAGGGC GATGGCCCAC TACGTGAACC ATCACCCAAA TCAAGTTTTT 8821 TGCGGTCGAG GTGCCGTAAA GCTCTAAATC GGAACCCTAA AGGGAGCCCC CGATTTAGAG 8881 CTTGACGGGG AAAGCCGGCG AACGTGGCGA GAAAGGAAGG GAAGAAAGCG AAAGGAGCGG 8941 GCGCTAGGGC GCTGGCAAGT GTAGCGGTCA CGCTGCGCGT AACCACCACA CCCGCGCGCT 9001 TAATGCGCCG NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 9061 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 9121 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 9181 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN //