出品公司: | Agilent |
---|---|
载体名称: | pAdTrack |
质粒类型: | 哺乳动物细胞腺病毒表达载体 |
高拷贝/低拷贝: | 低拷贝 |
启动子: | -- |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 8175 bp |
5' 测序引物及序列: | / |
3' 测序引物及序列: | / |
载体标签: | EGFP |
载体抗性: | 卡那 |
备注: | pAdTrack载体用来转基因腺病毒细胞表达,载体拥有一个EGFP绿色荧光蛋白跟踪基因表达结果,和pAdeasy1载体共同使用时最大能够插入5.9kb,pAdeasy2载体共同使用时能够最大插入8.6kb片段。具体使用方法可以参考pAdeasy载体系统。 |
产品目录号: | 240005 |
稳定性: | / |
组成型: | -- |
病毒/非病毒: | 腺病毒 |
买家导航
pAdTrack载体质粒基本信息
pAdTrack质粒图谱载体图谱和pAdTrack载体序列质粒序列多克隆位点信息
pAdTrack质粒载体简介
pAdTrack质粒序列载体序列
LOCUS pAdTrack 8175 bp DNA circular SYN DEFINITION pAdTrack ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI http://www.biofeng.com/ COMMENT VNTAUTHORNAME|biofeng.com| FEATURES Location/Qualifiers source 1..8175 /organism="pAdTrack" /mol_type="other DNA" misc_feature 197..345 /label="Encap" misc_feature complement(374..393) /label="EBV_rev_primer" CDS 575..1354 /label="ORF frame 2" CDS complement(593..1339) /label="ORF frame 3" gene complement(623..1336) /label="EGFP" /gene="EGFP" misc_feature complement(665..686) /label="EGFP_C_primer" misc_feature complement(1127..1156) /label="Y66 (EGFP)" misc_feature 1273..1294 /label="EGFP_N_primer" misc_feature complement(1413..1433) /label="CMV_fwd_primer" CDS complement(2563..3921) /label="ORF frame 1" CDS complement(3669..4190) /label="ORF frame 2" misc_feature complement(5147..5242) /label="LITR" misc_feature 5147..5242 /label="RITR" rep_origin complement(5487..6106) /label="pBR322_origin" gene complement(6261..6560) /label="Ampicillin (299 - 0)" /gene="Ampicillin (299 - 0)" promoter 6765..6814 /label="NEOKAN_promoter" CDS 6903..7697 /label="ORF frame 3" gene 6906..7694 /label="NeoR/KanR" /gene="NeoR/KanR" ORIGIN 1 NNNTTAATTA ANNNTCCCTT CCAGCTCTCT GCCCCTTTTG GATTGAAGCC AATATGATAA 61 TGAGGGGGTG GAGTTTGTGA CGTGGCGCGG GGCGTGGGAA CGGGGCGGGT GACGTAGTAG 121 TGTGGCGGAA GTGTGATGTT GCAAGTGTGG CGGAACACAT GTAAGCGACG GATGTGGCAA 181 AAGTGACGTT TTTGGTGTGC GCCGGTGTAC ACAGGAAGTG ACAATTTTCG CGCGGTTTTA 241 GGCGGATGTT GTAGTAAATT TGGGCGTAAC CGAGTAAGAT TTGGCCATTT TCGCGGGAAA 301 ACTGAATAAG AGGAAGTGAA ATCTGAATAA TTTTGTGTTA CTCATAGCGC GTAANNNCGC 361 GTTAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA ATGCAGTGAA AAAAATGCTT 421 TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC ATTATAAGCT GCAATAAACA 481 AGTTAACAAC AACAATTGCA TTCATTTTAT GTTTCAGGTT CAGGGGGAGG TGTGGGAGGT 541 TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTATGGCT GATTATGATC AGTTATCTAG 601 ATCCGGTGGA TCTGAGTCCG GACTTGTACA GCTCGTCCAT GCCGAGAGTG ATCCCGGCGG 661 CGGTCACGAA CTCCAGCAGG ACCATGTGAT CGCGCTTCTC GTTGGGGTCT TTGCTCAGGG 721 CGGACTGGGT GCTCAGGTAG TGGTTGTCGG GCAGCAGCAC GGGGCCGTCG CCGATGGGGG 781 TGTTCTGCTG GTAGTGGTCG GCGAGCTGCA CGCTGCCGTC CTCGATGTTG TGGCGGATCT 841 TGAAGTTCAC CTTGATGCCG TTCTTCTGCT TGTCGGCCAT GATATAGACG TTGTGGCTGT 901 TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA TGTTGCCGTC CTCCTTGAAG TCGATGCCCT 961 TCAGCTCGAT GCGGTTCACC AGGGTGTCGC CCTCGAACTT CACCTCGGCG CGGGTCTTGT 1021 AGTTGCCGTC GTCCTTGAAG AAGATGGTGC GCTCCTGGAC GTAGCCTTCG GGCATGGCGG 1081 ACTTGAAGAA GTCGTGCTGC TTCATGTGGT CGGGGTAGCG GCTGAAGCAC TGCACGCCGT 1141 AGGTCAGGGT GGTCACGAGG GTGGGCCAGG GCACGGGCAG CTTGCCGGTG GTGCAGATGA 1201 ACTTCAGGGT CAGCTTGCCG TAGGTGGCAT CGCCCTCGCC CTCGCCGGAC ACGCTGAACT 1261 TGTGGCCGTT TACGTCGCCG TCCAGCTCGA CCAGGATGGG CACCACCCCG GTGAACAGCT 1321 CCTCGCCCTT GCTCACCATG GTGGCGACCG GTAGCGCTAG CGGATCTGAC GGTTCACTAA 1381 ACCAGCTCTG CTTATATAGA CCTCCCACCG TACACGCCTA CCGCCCATTT GCGTCAATGG 1441 GGCGGAGTTG TTACGACATT TTGGAAAGTC CCGTTGATTT TGGTGCCAAA ACAAACTCCC 1501 ATTGACGTCA ATGGGGTGGA GACTTGGAAA TCCCCGTGAG TCAAACCGCT ATCCACGCCC 1561 ATTGATGTAC TGCCAAAACC GCATCACCAT GGTAATAGCG ATGACTAATA CGTAGATGTA 1621 CTGCCAAGTA GGAAAGTCCC ATAAGGTCAT GTACTGGGCA TAATGCCAGG CGGGCCATTT 1681 ACCGTCATTG ACGTCAATAG GGGGCGTACT TGGCATATGA TACACTTGAT GTACTGCCAA 1741 GTGGGCAGTT TACCGTAAAT ACTCCACCCA TTGACGTCAA TGGAAAGTCC CTATTGGCGT 1801 TACTATGGGA ACATACGTCA TTATTGACGT CAATGGGCGG GGGTCGTTGG GCGGTCAGCC 1861 AGGCGGGCCA TTTACCGTAA GTTATGTAAC GCGGAACTCC ATATATGGGC TATGAACTAA 1921 TGACCCCGTA ATTGATTACT ATTANNNCTA GCAGATCTGG TACCGTCGAC GCGGCCGCGA 1981 TATCCTCGAG AAGCTTTCTA GAGNNNTAAG GGTGGGAAAG AATATATAAG GTGGGGGTCT 2041 TATGTAGTTT TGTATCTGTT TTGCAGCAGC CGCCGCCGCC ATGAGCACCA ACTCGTTTGA 2101 TGGAAGCATT GTGAGCTCAT ATTTGACAAC GCGCATGCCC CCATGGGCCG GGGTGCGTCA 2161 GAATGTGATG GGCTCCAGCA TTGATGGTCG CCCCGTCCTG CCCGCAAACT CTACTACCTT 2221 GACCTACGAG ACCGTGTCTG GAACGCCGTT GGAGACTGCA GCCTCCGCCG CCGCTTCAGC 2281 CGCTGCAGCC ACCGCCCGCG GGATTGTGAC TGACTTTGCT TTCCTGAGCC CGCTTGCAAG 2341 CAGTGCAGCT TCCCGTTCAT CCGCCCGCGA TGACAAGTTG ACGGCTCTTT TGGCACAATT 2401 GGATTCTTTG ACCCGGGAAC TTAATGTCGT TTCTCAGCAG CTGTTGGATC TGCGCCAGCA 2461 GGTTTCTGCC CTGAAGGCTT CCTCCCCTCC CAATGCGGTT TAAAACATAA ATAAAAAACC 2521 AGACTCTGTT TGGATTTGGA TCAAGCAAGT GTCTTGCTGT CTTTATTTAG GGGTTTTGCG 2581 CGCGCGGTAG GCCCGGGACC AGCGGTCTCG GTCGTTGAGG GTCCTGTGTA TTTTTTCCAG 2641 GACGTGGTAA AGGTGACTCT GGATGTTCAG ATACATGGGC ATAAGCCCGT CTCTGGGGTG 2701 GAGGTAGCAC CACTGCAGAG CTTCATGCTG CGGGGTGGTG TTGTAGATGA TCCAGTCGTA 2761 GCAGGAGCGC TGGGCGTGGT GCCTAAAAAT GTCTTTCAGT AGCAAGCTGA TTGCCAGGGG 2821 CAGGCCCTTG GTGTAAGTGT TTACAAAGCG GTTAAGCTGG GATGGGTGCA TACGTGGGGA 2881 TATGAGATGC ATCTTGGACT GTATTTTTAG GTTGGCTATG TTCCCAGCCA TATCCCTCCG 2941 GGGATTCATG TTGTGCAGAA CCACCAGCAC AGTGTATCCG GTGCACTTGG GAAATTTGTC 3001 ATGTAGCTTA GAAGGAAATG CGTGGAAGAA CTTGGAGACG CCCTTGTGAC CTCCAAGATT 3061 TTCCATGCAT TCGTCCATAA TGATGGCAAT GGGCCCACGG GCGGCGGCCT GGGCGAAGAT 3121 ATTTCTGGGA TCACTAACGT CATAGTTGTG TTCCAGGATG AGATCGTCAT AGGCCATTTT 3181 TACAAAGCGC GGGCGGAGGG TGCCAGACTG CGGTATAATG GTTCCATCCG GCCCAGGGGC 3241 GTAGTTACCC TCACAGATTT GCATTTCCCA CGCTTTGAGT TCAGATGGGG GGATCATGTC 3301 TACCTGCGGG GCGATGAAGA AAACGGTTTC CGGGGTAGGG GAGATCAGCT GGGAAGAAAG 3361 CAGGTTCCTG AGCAGCTGCG ACTTACCGCA GCCGGTGGGC CCGTAAATCA CACCTATTAC 3421 CGGGTGCAAC TGGTAGTTAA GAGAGCTGCA GCTGCCGTCA TCCCTGAGCA GGGGGGCCAC 3481 TTCGTTAAGC ATGTCCCTGA CTCGCATGTT TTCCCTGACC AAATCCGCCA GAAGGCGCTC 3541 GCCGCCCAGC GATAGCAGTT CTTGCAAGGA AGCAAAGTTT TTCAACGGTT TGAGACCGTC 3601 CGCCGTAGGC ATGCTTTTGA GCGTTTGACC AAGCAGTTCC AGGCGGTCCC ACAGCTCGGT 3661 CACCTGCTCT ACGGCATCTC GATCCAGCAT ATCTCCTCGT TTCGCGGGTT GGGGCGGCTT 3721 TCGCTGTACG GCAGTAGTCG GTGCTCGTCC AGACGGGCCA GGGTCATGTC TTTCCACGGG 3781 CGCAGGGTCC TCGTCAGCGT AGTCTGGGTC ACGGTGAAGG GGTGCGCTCC GGGCTGCGCG 3841 CTGGCCAGGG TGCGCTTGAG GCTGGTCCTG CTGGTGCTGA AGCGCTGCCG GTCTTCGCCC 3901 TGCGCGTCGG CCAGGTAGCA TTTGACCATG GTGTCATAGT CCAGCCCCTC CGCGGCGTGG 3961 CCCTTGGCGC GCAGCTTGCC CTTGGAGGAG GCGCCGCACG AGGGGCAGTG CAGACTTTTG 4021 AGGGCGTAGA GCTTGGGCGC GAGAAATACC GATTCCGGGG AGTAGGCATC CGCGCCGCAG 4081 GCCCCGCAGA CGGTCTCGCA TTCCACGAGC CAGGTGAGCT CTGGCCGTTC GGGGTCAAAA 4141 ACCAGGTTTC CCCCATGCTT TTTGATGCGT TTCTTACCTC TGGTTTCCAT GAGCCGGTGT 4201 CCACGCTCGG TGACGAAAAG GCTGTCCGTG TCCCCGTATA CAGACTNNNG TTTAAACGAA 4261 TTCNNNTATA AAATGCAAGG TGCTGCTCAA AAAATCAGGC AAAGCCTCGC GCAAAAAAGA 4321 AAGCACATCG TAGTCATGCT CATGCAGATA AAGGCAGGTA AGCTCCGGAA CCACCACAGA 4381 AAAAGACACC ATTTTTCTCT CAAACATGTC TGCGGGTTTC TGCATAAACA CAAAATAAAA 4441 TAACAAAAAA ACATTTAAAC ATTAGAAGCC TGTCTTACAA CAGGAAAAAC AACCCTTATA 4501 AGCATAAGAC GGACTACGGC CATGCCGGCG TGACCGTAAA AAAACTGGTC ACCGTGATTA 4561 AAAAGCACCA CCGACAGCTC CTCGGTCATG TCCGGAGTCA TAATGTAAGA CTCGGTAAAC 4621 ACATCAGGTT GATTCATCGG TCAGTGCTAA AAAGCGACCG AAATAGCCCG GGGGAATACA 4681 TACCCGCAGG CGTAGAGACA ACATTACAGC CCCCATAGGA GGTATAACAA AATTAATAGG 4741 AGAGAAAAAC ACATAAACAC CTGAAAAACC CTCCTGCCTA GGCAAAATAG CACCCTCCCG 4801 CTCCAGAACA ACATACAGCG CTTCACAGCG GCAGCCTAAC AGTCAGCCTT ACCAGTAAAA 4861 AAGAAAACCT ATTAAAAAAA CACCACTCGA CACGGCACCA GCTCAATCAG TCACAGTGTA 4921 AAAAAGGGCC AAGTGCAGAG CGAGTATATA TAGGACTAAA AAATGACGTA ACGGTTAAAG 4981 TCCACAAAAA ACACCCAGAA AACCGCACGC GAACCTACGC CCAGAAACGA AAGCCAAAAA 5041 ACCCACAACT TCCTCAAATC GTCACTTCCG TTTTCCCACG TTACGTAACT TCCCATTTTA 5101 AGAAAACTAC AATTCCCAAC ACATACAAGT TACTCCGCCC TAAAACCTAC GTCACCCGCC 5161 CCGTTCCCAC GCCCCGCGCC ACGTCACAAA CTCCACCCCC TCATTATCAT ATTGGCTTCA 5221 ATCCAAAATA AGGTATATTA TTGATGATNN NTTAATTAAG GATCCNNNCG GTGTGAAATA 5281 CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCT CTTCCGCTTC CTCGCTCACT 5341 GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA 5401 ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG 5461 CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC 5521 CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA 5581 TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG 5641 CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC 5701 TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC 5761 GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC 5821 CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG 5881 AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA 5941 AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT 6001 AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG 6061 CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT 6121 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG 6181 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT 6241 GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC 6301 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG 6361 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT 6421 CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA 6481 ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG 6541 CCAGTTAATA GTTTGCGCAA CGTTGTTGNN NNNNAAAAAG GATCTTCACC TAGATCCTTT 6601 TCACGTAGAA AGCCAGTCCG CAGAAACGGT GCTGACCCCG GATGAATGTC AGCTACTGGG 6661 CTATCTGGAC AAGGGAAAAC GCAAGCGCAA AGAGAAAGCA GGTAGCTTGC AGTGGGCTTA 6721 CATGGCGATA GCTAGACTGG GCGGTTTTAT GGACAGCAAG CGAACCGGAA TTGCCAGCTG 6781 GGGCGCCCTC TGGTAAGGTT GGGAAGCCCT GCAAAGTAAA CTGGATGGCT TTCTCGCCGC 6841 CAAGGATCTG ATGGCGCAGG GGATCAAGCT CTGATCAAGA GACAGGATGA GGATCGTTTC 6901 GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT 6961 TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT 7021 CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC 7081 TGCAAGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG 7141 TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC 7201 AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA 7261 TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC 7321 GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG 7381 AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG AGCATGCCCG 7441 ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA 7501 ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG 7561 ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT 7621 TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC 7681 TTGACGAGTT CTTCTGAATT TTGTTAAAAT TTTTGTTAAA TCAGCTCATT TTTTAACCAA 7741 TAGGCCGAAA TCGGCAACAT CCCTTATAAA TCAAAAGAAT AGACCGCGAT AGGGTTGAGT 7801 GTTGTTCCAG TTTGGAACAA GAGTCCACTA TTAAAGAACG TGGACTCCAA CGTCAAAGGG 7861 CGAAAAACCG TCTATCAGGG CGATGGCCCA CTACGTGAAC CATCACCCAA ATCAAGTTTT 7921 TTGCGGTCGA GGTGCCGTAA AGCTCTAAAT CGGAACCCTA AAGGGAGCCC CCGATTTAGA 7981 GCTTGACGGG GAAAGCCGGC GAACGTGGCG AGAAAGGAAG GGAAGAAAGC GAAAGGAGCG 8041 GGCGCTAGGG CGCTGGCAAG TGTAGCGGTC ACGCTGCGCG TAACCACCAC ACCCGCGCGC 8101 TTAATGCGCC GNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 8161 NNNNNNNNNN NNNNN //
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pAdTrack质粒载体应用举例
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