| 出品公司: | Agilent Technologies |
|---|---|
| 载体名称: | pNTAP-A , pNTAPA |
| 质粒类型: | 哺乳细胞表达系统载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | CMV |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 4530bp |
| 5' 测序引物及序列: | CMV-F: CGCAAATGGGCGGTAGGCGTG |
| 3' 测序引物及序列: | SV40pA-R: GAAATTTGTGATGCTATTGC |
| 载体标签: | N端-streptavidin结合多肽 ; N端-calmodulin结合肽段 |
| 载体抗性: | 卡那霉素 |
| 筛选标记: | 新霉素 |
| 备注: | 能够表达N端含有能结合链霉亲和素的多肽片段，以及N端结合钙调蛋白结合多肽片段的载体. |
| 产品目录号: | -- |
| 稳定性: | 诱导表达 |
| 组成型: | 非组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pNTAPA载体基本信息
pNTAPA载体图谱质粒图谱和多克隆位点信息
pNTAPA载体简介
pNTAPA载体序列
LOCUS pNTAP-A 4530 bp ds-DNA circular SYN 28-十一月-2013
DEFINITION Vector for expressing proteins N-terminally tagged with calmodulin-
and streptavidin-binding peptides.?For other reading frames, use
pNTAP-B or pNTAP-C.
ACCESSION .
VERSION .
KEYWORDS pNTAP-A
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..4530
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer 66..370
/label="CMV enhancer"
/label="human cytomegalovirus immediate early enhancer"
promoter 371..574
/label="CMV promoter"
/label="human cytomegalovirus (CMV) immediate early
promoter"
promoter 620..638
/label="T3 promoter"
/label="promoter for bacteriophage T3 RNA polymerase"
CDS 679..681
/codon_start=1
/product="start codon"
/label="ATG"
/translation="M"
CDS 682..759
/codon_start=1
/product="calmodulin-binding peptide"
/label="CBP"
/label="derived from skeletal muscle myosin light chain
kinase; binds calmodulin with nanomolar affinity in the
presence of calcium"
/translation="KRRWKKNFIAVSAANRFKKISSSGAL"
CDS 778..891
/codon_start=1
/product="streptavidin-binding peptide"
/label="SBP"
/label="selected from a peptide library; binds streptavidin
with nanomolar affinity"
/translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
misc_feature 913..986
/label="MCS"
/label="multiple cloning site"
misc_feature 994..1004
/label="stop codons"
/label="stop codons in all three reading frames"
promoter complement(1032..1050)
/label="T7 promoter"
/label="promoter for bacteriophage T7 RNA polymerase"
polyA_signal 1324..1445
/label="SV40 poly(A) signal"
/label="SV40 polyadenylation signal"
rep_origin complement(1452..1907)
/direction=LEFT
/label="f1 ori"
/label="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1934..2036
/gene="bla"
/label="AmpR promoter"
promoter 2040..2397
/label="SV40 promoter"
/label="SV40 enhancer and early promoter"
CDS 2432..3226
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label="NeoR/KanR"
/label="confers resistance to neomycin, kanamycin, and G418
(Geneticin)"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
misc_feature 3458..3505
/label="HSV TK poly(A) signal"
/label="herpesvirus thymidine kinase polyadenylation signal"
rep_origin 3834..4422
/direction=RIGHT
/label="ori"
/label="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc
661 gcggtggcgg ccgccaccat gaagcgacga tggaaaaaga atttcatagc cgtctcagca
721 gccaaccgct ttaagaaaat ctcatcctcc ggggcacttg gaagcggtag cggtaccatg
781 gacgagaaga ccaccggctg gcggggcggc cacgtggtgg agggcctggc cggcgagctg
841 gagcagctgc gggccaggct ggagcaccac cctcagggcc agcgggagcc ctccggcggc
901 tgcaagctgg gcgcccgggc ggatcccccg ggctgcagga attcgatatc aagcttatcg
961 ataccgtcga cctcgagggg gggcccggta ccttaattaa ttaaggtacc aggtaagtgt
1021 acccaattcg ccctatagtg agtcgtatta caattcactc gatcgccctt cccaacagtt
1081 gcgcagcctg aatggcgaat ggagatccaa tttttaagtg tataatgtgt taaactactg
1141 attctaattg tttgtgtatt ttagattcac agtcccaagg ctcatttcag gcccctcagt
1201 cctcacagtc tgttcatgat cataatcagc cataccacat ttgtagaggt tttacttgct
1261 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
1321 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
1381 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
1441 tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
1501 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
1561 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
1621 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
1681 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
1741 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
1801 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
1861 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt
1921 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
1981 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagaatcc
2041 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
2101 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
2161 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
2221 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
2281 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
2341 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat
2401 cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
2461 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
2521 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
2581 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct
2641 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
2701 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
2761 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
2821 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
2881 cggtcttgtc gatcaggatg atctggacga agaacatcag gggctcgcgc cagccgaact
2941 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
3001 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
3061 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
3121 agaacttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
3181 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
3241 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
3301 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
3361 cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca
3421 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa
3481 acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct
3541 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca
3601 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc
3661 cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa
3721 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
3781 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
3841 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
3901 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
3961 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
4021 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
4081 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
4141 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
4201 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
4261 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
4321 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
4381 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
4441 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
4501 cctgattctg tggataaccg tattaccgcc
//
