出品公司: | ATCC |
---|---|
载体名称: | pSV2DHFR, pSV2-DHFR, ATCC37146, ATCC 37146 |
质粒类型: | 哺乳动物细胞表达载体 |
启动子: | SV40 |
表达水平: | 高 |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 4900 bp |
5' 测序引物: | SV40pro-F: TATTTATGCAGAGGCCGAGG |
3' 测序引物: | SV40pA-R: GAAATTTGTGATGCTATTGC |
载体标签: | C-DHFR ( mouse DHFR) |
载体抗性: | 氨苄 |
筛选标记: | MTX (甲氨蝶呤) |
备注: |
pSV2-DHFR载体含有的是小鼠的DHFR基因,同时该载体是哺乳动物细胞和大肠杆菌穿梭质粒,在DHFR细胞系中,可以通过甲氨蝶呤对转染的细胞进行筛选。
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产品目录号: | 37146 |
稳定性: | 稳表达 |
组成型: | 组成型 |
病毒/非病毒: | 非病毒 |
买家导航
pSV2-DHFR载体 pSV2DHFR质粒基本信息
pSV2-DHFR质粒图谱 pSV2DHFR载体图谱和pSV2-DHFR载体序列 pSV2DHFR质粒序列多克隆位点信息
pSV2-DHFR质粒 pSV2DHFR载体简介
pSV2-DHFR质粒序列 pSV2DHFR载体序列
LOCUS pSV2-DHFR 4900 bp DNA circular SYN DEFINITION pSV2-DHFR ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI http://www.biofeng.com/ COMMENT VNTAUTHORNAME|biofeng.com| FEATURES Location/Qualifiers source 1..4900 /organism="pSV2-dhfr" /mol_type="other DNA" misc_feature complement(5..25) /label="pBABE_3_primer" misc_feature complement(11..226) /label="SV40_enhancer" promoter 23..291 /label="SV40_promoter" rep_origin 190..267 /label="SV40_origin" misc_feature 252..271 /label="SV40pro_F_primer" CDS 362..925 /label="mouse DHFR" misc_feature 1081..1096 /label="SV40_int" misc_feature 1102..1149 /label="SV40_3_splice" CDS 1226..1699 /label="ORF frame 2" misc_feature 1277..1297 /label="SV40 NLS" misc_feature 1283..1297 /label="NLS" terminator 1725..1856 /label="SV40_PA_terminator" CDS complement(1797..2558) /label="ORF frame 3" misc_feature 1813..1832 /label="EBV_rev_primer" promoter 2744..2772 /label="AmpR_promoter" gene 2814..3674 /label="Ampicillin" /gene="Ampicillin" CDS 2814..3674 /label="ORF frame 3" rep_origin 3829..4448 /label="pBR322_origin" misc_feature 4845..4867 /label="pGEX_3_primer" ORIGIN 1 cTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 61 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA 121 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA 181 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA 241 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG 301 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTTTATCC CCGCTGCCAT 361 CATGGTTCGA CCATTGAACT GCATCGTCGC CGTGTCCCAA GATATGGGGA TTGGCAAGAA 421 CGGAGACCTA CCCTGGCCTC CGCTCAGGAA CGAGTTCAAG TACTTCCAAA GAATGACCAC 481 AACCTCTTCA GTGGAAGGTA AACAGAATCT GGTGATTATG GGTAGGAAAA CCTGGTTCTC 541 CATTCCTGAG AAGAATCGAC CTTTAAAGGA CAGAATTAAT ATAGTTCTCA GTAGAGAACT 601 CAAAGAACCA CCACGAGGAG CTCATTTTCT TGCCAAAAGT TTGGATGATG CCTTAAGACT 661 TATTGAACAA CCGGAATTGG CAAGTAAAGT AGACATGGTT TGGATAGTCG GAGGCAGTTC 721 TGTTTACCAG GAAGCCATGA ATCAACCAGG CCACCTCAGA CTCTTTGTGA CAAGGATCAT 781 GCAGGAATTT GAAAGTGACA CGTTTTTCCC AGAAATTGAT TTGGGGAAAT ATAAACTTCT 841 CCCAGAATAC CCAGGCGTCC TCTCTGAGGT CCAGGAGGAA AAAGGCATCA AGTATAAGTT 901 TGAAGTCTAC GAGAAGAAAG ACTAACAGGA AGATGCTTTC AAGTTCTCTG CTCCCCTCCT 961 AAAGCTATGC ATTTTTATAA GACCATGGGA CTTTTGCTGG CTTTAGATCT TTGTGAAGGA 1021 ACCTTACTTC TGTGGTGTGA CATAATTGGA CAAACTACCT ACAGAGATTT AAAGCTCTAA 1081 GGTAAATATA AAATTTTTAA GTGTATAATG TGTTAAACTA CTGATTCTAA TTGTTTGTGT 1141 ATTTTAGATT CCAACCTATG GAACTGATGA ATGGGAGCAG TGGTGGAATG CCTTTAATGA 1201 GGAAAACCTG TTTTGCTCAG AAGAAATGCC ATCTAGTGAT GATGAGGCTA CTGCTGACTC 1261 TCAACATTCT ACTCCTCCAA AAAAGAAGAG AAAGGTAGAA GACCCCAAGG ACTTTCCTTC 1321 AGAATTGCTA AGTTTTTTGA GTCATGCTGT GTTTAGTAAT AGAACTCTTG CTTGCTTTGC 1381 TATTTACACC ACAAAGGAAA AAGCTGCACT GCTATACAAG AAAATTATGG AAAAATATTC 1441 TGTAACCTTT ATAAGTAGGC ATAACAGTTA TAATCATAAC ATACTGTTTT TTCTTACTCC 1501 ACACAGGCAT AGAGTGTCTG CTATTAATAA CTATGCTCAA AAATTGTGTA CCTTTAGCTT 1561 TTTAATTTGT AAAGGGGTTA ATAAGGAATA TTTGATGTAT AGTGCCTTGA CTAGAGATCA 1621 TAATCAGCCA TACCACATTT GTAGAGGTTT TACTTGCTTT AAAAAACCTC CCACACCTCC 1681 CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT ATTGCAGCTT 1741 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC 1801 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGGATCCCCA 1861 GGAAGCTCCT CTGTGTCCTC ATAAACCCTA ACCTCCTCTA CTTGAGAGGA CATTCCAATC 1921 ATAGGCTGCC CATCCACCCT CTGTGTCCTC CTGTTAATTA GGTCACTTAA CAAAAAGGAA 1981 ATTGGGTAGG GGTTTTTCAC AGACCGCTTT CTAAGGGTAA TTTTAAAATA TCTGGGAAGT 2041 CCCTTCCACT GCTGTGTTCC AGAAGTGTTG GTAAACAGCC CACAAATGTC AACAGCAGAA 2101 ACATACAAGC TGTCAGCTTT GCACAAGGGC CCAACACCCT GCTCATCAAG AAGCACTGTG 2161 GTTGCTGTGT TAGTAATGTG CAAAACAGGA GGCACATTTT CCCCACCTGT GTAGGTTCCA 2221 AAATATCTAG TGTTTTCATT TTTACTTGGA TCAGGAACCC AGCACTCCAC TGGATAAGCA 2281 TTATCCTTAT CCAAAACAGC CTTGTGGTCA GTGTTCATCT GCTGACTGTC AACTGTAGCA 2341 TTTTTTGGGG TTACAGTTTG AGCAGGATAT TTGGTCCTGT AGTTTGCTAA CACACCCTGC 2401 AGCTCCAAAG GTTCCCCACC AACAGCAAAA AAATGAAAAT TTGACCCTTG AATGGGTTTT 2461 CCAGCACCAT TTTCATGAGT TTTTTGTGTC CCTGAATGCA AGTTTAACAT AGCAGTTACC 2521 CCAATAACCT CAGTTTTAAC AGTAACAGCT TCCCACATCA AAATATTTCC ACAGGTTAAG 2581 TCCTCATTTA AATTAGGCAA AGGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT 2641 TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG 2701 GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC 2761 TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA 2821 TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG 2881 CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG 2941 GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC 3001 GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG 3061 ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT 3121 ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG 3181 CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC 3241 CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT 3301 GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG 3361 CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC 3421 AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC 3481 TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA 3541 TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG 3601 GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA 3661 TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC 3721 TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA 3781 TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT 3841 CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC 3901 TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG 3961 GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC 4021 ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG 4081 CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG 4141 ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA 4201 CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG 4261 AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA 4321 GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT 4381 GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA 4441 GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC 4501 CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG 4561 CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC 4621 TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TGGTGCACTC 4681 TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG 4741 TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC 4801 TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG 4861 TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG //
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pSV2-DHFR质粒 pSV2DHFR载体应用举例
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