| 出品公司: | Agilent Technologies |
|---|---|
| 载体名称: | pNTAP-C, pNTAPC |
| 质粒类型: | 哺乳细胞表达系统载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | CMV |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 4532bp |
| 5' 测序引物及序列: | CMV-F: CGCAAATGGGCGGTAGGCGTG |
| 3' 测序引物及序列: | SV40pA-R: GAAATTTGTGATGCTATTGC |
| 载体标签: | streptavidin-binding peptides ;calmodulin-binding peptides |
| 载体抗性: | 卡那霉素 |
| 筛选标记: | 新霉素 |
| 备注: | 能够表达N端含有能结合链霉亲和素的多肽片段，以及N端结合钙调蛋白结合多肽片段的载体. |
| 产品目录号: | -- |
| 稳定性: | 诱导表达 |
| 组成型: | 非组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pNTAPC载体基本信息
pNTAPC载体图谱质粒图谱和多克隆位点信息
pNTAPC载体简介
pNTAPC载体序列
LOCUS pNTAP-C 4532 bp ds-DNA circular SYN 28-十一月-2013
DEFINITION Vector for expressing proteins N-terminally tagged with calmodulin-
and streptavidin-binding peptides.?For other reading frames, use
pNTAP-A or pNTAP-B.
ACCESSION .
VERSION .
KEYWORDS pNTAP-C
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..4532
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer 66..370
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 371..574
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 620..638
/note="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
CDS 679..681
/codon_start=1
/product="start codon"
/note="ATG"
/translation="M"
CDS 682..759
/codon_start=1
/product="calmodulin-binding peptide"
/note="CBP"
/note="derived from skeletal muscle myosin light chain
kinase; binds calmodulin with nanomolar affinity in the
presence of calcium"
/translation="KRRWKKNFIAVSAANRFKKISSSGAL"
CDS 778..891
/codon_start=1
/product="streptavidin-binding peptide"
/note="SBP"
/note="selected from a peptide library; binds streptavidin
with nanomolar affinity"
/translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
misc_feature 915..988
/note="MCS"
/note="multiple cloning site"
misc_feature 996..1006
/note="stop codons"
/note="stop codons in all three reading frames"
promoter complement(1034..1052)
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
polyA_signal 1326..1447
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(1454..1909)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1936..2038
/gene="bla"
/note="AmpR promoter"
promoter 2042..2399
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
CDS 2434..3228
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin)"
misc_feature 3460..3507
/note="HSV TK poly(A) signal"
/note="herpesvirus thymidine kinase polyadenylation signal"
rep_origin 3836..4424
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc
661 gcggtggcgg ccgccaccat gaagcgacga tggaaaaaga atttcatagc cgtctcagca
721 gccaaccgct ttaagaaaat ctcatcctcc ggggcacttg gaagcggtag cggtaccatg
781 gacgagaaga ccaccggctg gcggggcggc cacgtggtgg agggcctggc cggcgagctg
841 gagcagctgc gggccaggct ggagcaccac cctcagggcc agcgggagcc ctccggcggc
901 tgcaagctgg gcttgcccgg gcggatcccc cgggctgcag gaattcgata tcaagcttat
961 cgataccgtc gacctcgagg gggggcccgg taccttaatt aattaaggta ccaggtaagt
1021 gtacccaatt cgccctatag tgagtcgtat tacaattcac tcgatcgccc ttcccaacag
1081 ttgcgcagcc tgaatggcga atggagatcc aatttttaag tgtataatgt gttaaactac
1141 tgattctaat tgtttgtgta ttttagattc acagtcccaa ggctcatttc aggcccctca
1201 gtcctcacag tctgttcatg atcataatca gccataccac atttgtagag gttttacttg
1261 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
1321 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
1381 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
1441 tatcttaacg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
1501 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
1561 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
1621 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
1681 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
1741 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
1801 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
1861 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact
1921 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
1981 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagaat
2041 cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
2101 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
2161 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
2221 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
2281 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
2341 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag
2401 atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
2461 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
2521 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
2581 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg
2641 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
2701 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
2761 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
2821 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
2881 gccggtcttg tcgatcagga tgatctggac gaagaacatc aggggctcgc gccagccgaa
2941 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc
3001 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
3061 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
3121 gaagaacttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
3181 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
3241 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
3301 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
3361 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa
3421 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
3481 aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact
3541 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc
3601 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag
3661 gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt
3721 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
3781 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
3841 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
3901 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
3961 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
4021 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
4081 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
4141 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
4201 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
4261 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
4321 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
4381 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
4441 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
4501 cccctgattc tgtggataac cgtattaccg cc
//
