| 质粒名称: | pFUS_B1 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 31018 |
| 存储实验室: | Adam Bogdanove, Daniel Voytas |
|
载体骨架基本信息 |
|
| 载体名称: | pCR8 |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 大观霉素 |
| 空载体大小: | 2447 bp |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | LacZ + BsaI restriction sites |
| 插入大小: | 459 bp |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AflII (未破坏) |
| 3' 克隆位点: | XbaI(未破坏) |
| 5' 测序引物 | pCR8_F1 (ttgatgcctggcagttccct) |
| 3' 测序引物 | pCR8_R1 (cgaaccgaacaggcttatgt) |
|
文献引用方法 |
|
| 材料和方法部分: | pFUS_B1 was a gift from Adam Bogdanove & Daniel Voytas (Addgene plasmid # 31018 ; http://n2t.net/addgene:31018 ; RRID:Addgene_31018) |
| 参考文献部分: | Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. 2011 Apr 14. ():. 10.1093/nar/gkr218 PubMed 21493687 |
买家导航
pFUSB1载体基本信息
pFUSB1载体图谱质粒图谱和多克隆位点信息
pFUSB1载体简介
pFUSB1载体序列
LOCUS pFUS_B1 2984 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pFUS_B1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2984)
AUTHORS Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller
JA, Somia NV, Bogdanove AJ, Voytas DF
TITLE Efficient design and assembly of custom TALEN and other TAL
effector-based constructs for DNA targeting.
JOURNAL Nucleic Acids Res. 2011 Apr 14. ():.
PUBMED 21493687
REFERENCE 2 (bases 1 to 2984)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..2984
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 206..222
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 232..251
/label=T7
/note="T7 promoter, forward primer"
promoter 232..250
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(298..318)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(298..316)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(337..353)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(337..353)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(350..372)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 361..377
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(385..415)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 430..451
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
CDS 891..1682
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 1775..2363
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2264..2283
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 2517..2534
/label=L4440
/note="L4440 vector, forward primer"
terminator 2693..2720
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 2812..2898
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
CDS complement(2819..341)
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
/translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGLDSH
HLVRGQAMRRLRLAVVLQRRDWENIHASVNARVGNCQASNKTKGSVGRLGLSFYLLFVG
ERSPE"
primer_bind 2962..2978
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 cgtctcatgg cctgaccccg gaccaagtgg tggctatcga gaccggcgcc gctacagggc
61 gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
121 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
181 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac
241 tcactatagg gcgaattggg taccgggccc cccctcgagg tcctccagct tttgttccct
301 ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
361 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
421 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
481 ccggtggtct cccatggcct gaccccggac caagtggtgg ctatcgagac gtctagacca
541 gccaggacag aaatgcctcg acttcgctgc tacccaaggt tgccgggtga cgcacaccgt
601 ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
661 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
721 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
781 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
841 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatt atgagggaag
901 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
961 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
1021 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
1081 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
1141 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
1201 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
1261 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
1321 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
1381 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
1441 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
1501 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
1561 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
1621 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
1681 aaccctcgag ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag
1741 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
1801 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
1861 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
1921 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
1981 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
2041 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
2101 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
2161 gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
2221 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
2281 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
2341 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
2401 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
2461 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
2521 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
2581 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
2641 gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc
2701 atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc
2761 ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct
2821 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg
2881 agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg ctagcatgga
2941 tgttttccca gtcacgacgt tgtaaaacga cggccagtct taag
//
