| 质粒名称: | pFUS_A30B |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 31030 |
| 存储实验室: | Adam Bogdanove, Daniel Voytas |
|
载体骨架基本信息 |
|
| 载体名称: | pCR8 |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 大观霉素 |
| 空载体大小: | 2447 bp |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | LacZ + BsaI restriction sites |
| 插入大小: | 459 bp |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AflII (未破坏) |
| 3' 克隆位点: | XbaI(未破坏) |
| 5' 测序引物 | pCR8_F1 (ttgatgcctggcagttccct) |
| 3' 测序引物 | pCR8_R1 (cgaaccgaacaggcttatgt) |
|
文献引用方法 |
|
| 材料和方法部分: | pFUS_A30B was a gift from Adam Bogdanove & Daniel Voytas (Addgene plasmid # 31030 ; http://n2t.net/addgene:31030 ; RRID:Addgene_31030) |
| 参考文献部分: | Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. 2011 Apr 14. ():. 10.1093/nar/gkr218 PubMed 21493687 |
买家导航
pFUSA30B载体基本信息
pFUSA30B载体图谱质粒图谱和多克隆位点信息
pFUSA30B载体简介
pFUSA30B载体序列
LOCUS pFUS_A30B 3011 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pFUS_A30B
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3011)
AUTHORS Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller
JA, Somia NV, Bogdanove AJ, Voytas DF
TITLE Efficient design and assembly of custom TALEN and other TAL
effector-based constructs for DNA targeting.
JOURNAL Nucleic Acids Res. 2011 Apr 14. ():.
PUBMED 21493687
REFERENCE 2 (bases 1 to 3011)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3011
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 202..218
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 228..247
/label=T7
/note="T7 promoter, forward primer"
promoter 228..246
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(294..314)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(294..312)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(333..349)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(333..349)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(346..368)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 357..373
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(381..411)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 426..447
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
CDS 918..1709
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 1802..2390
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2291..2310
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 2544..2561
/label=L4440
/note="L4440 vector, forward primer"
terminator 2720..2747
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 2839..2925
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
primer_bind 2989..3005
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
CDS complement(3007..337)
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
/translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGLDSH
HLVRGQGDA"
ORIGIN
1 cgtctccctg accccggacc aagtggtggc tatcgagacc ggcgccgcta cagggcgcgt
61 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
121 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
181 ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac
241 tatagggcga attgggtacc gggccccccc tcgaggtcct ccagcttttg ttccctttag
301 tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
361 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
421 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccaccgg
481 tggtctctgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc
541 aggaccatgg ccgagacgtc tagaccagcc aggacagaaa tgcctcgact tcgctgctac
601 ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat
661 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
721 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
781 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
841 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
901 aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag
961 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
1021 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
1081 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
1141 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
1201 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
1261 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
1321 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
1381 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
1441 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
1501 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
1561 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
1621 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag
1681 gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta
1741 acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
1801 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
1861 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
1921 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
1981 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
2041 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
2101 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
2161 cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag
2221 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
2281 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
2341 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
2401 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
2461 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
2521 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
2581 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
2641 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc gctagccagg
2701 aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg
2761 cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt
2821 caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
2881 aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc tggcagttcc
2941 ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgttgt aaaacgacgg
3001 ccagtcttaa g
//
