| 质粒名称: | pFUS_A30A |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 31029 |
| 存储实验室: | Adam Bogdanove, Daniel Voytas |
|
载体骨架基本信息 |
|
| 载体名称: | pCR8 |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 大观霉素 |
| 空载体大小: | 2447 bp |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | LacZ + BsaI restriction sites |
| 插入大小: | 459 bp |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AflII (未破坏) |
| 3' 克隆位点: | XbaI(未破坏) |
| 5' 测序引物 | pCR8_F1 (ttgatgcctggcagttccct) |
| 3' 测序引物 | pCR8_R1 (cgaaccgaacaggcttatgt) |
|
文献引用方法 |
|
| 材料和方法部分: | pFUS_A30A was a gift from Adam Bogdanove & Daniel Voytas (Addgene plasmid # 31029 ; http://n2t.net/addgene:31029 ; RRID:Addgene_31029) |
| 参考文献部分: | Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. 2011 Apr 14. ():. 10.1093/nar/gkr218 PubMed 21493687 |
买家导航
pFUSA30A载体基本信息
pFUSA30A载体图谱质粒图谱和多克隆位点信息
pFUSA30A载体简介
pFUSA30A载体序列
LOCUS pFUS_A30A 3023 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pFUS_A30A
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3023)
AUTHORS Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller
JA, Somia NV, Bogdanove AJ, Voytas DF
TITLE Efficient design and assembly of custom TALEN and other TAL
effector-based constructs for DNA targeting.
JOURNAL Nucleic Acids Res. 2011 Apr 14. ():.
PUBMED 21493687
REFERENCE 2 (bases 1 to 3023)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3023
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 210..226
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 236..255
/label=T7
/note="T7 promoter, forward primer"
promoter 236..254
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(302..322)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(302..320)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(341..357)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(341..357)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(354..376)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 365..381
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(389..419)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 434..455
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
CDS 930..1721
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 1814..2402
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2303..2322
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 2556..2573
/label=L4440
/note="L4440 vector, forward primer"
terminator 2732..2759
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 2851..2937
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
CDS complement(2991..345)
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
/translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGLDSH
HLVRGQVQGETLKTGRRFTTS"
primer_bind 3001..3017
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 cgtctccccc tgaacctgac cccggaccaa gtggtggcta tcgagaccgg cgccgctaca
61 gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
121 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
181 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata
241 cgactcacta tagggcgaat tgggtaccgg gccccccctc gaggtcctcc agcttttgtt
301 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
361 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
421 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
481 tccaccggtg gtctctggcg gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt
541 gctgtgccag gaccatggcc tgacgagacg tctagaccag ccaggacaga aatgcctcga
601 cttcgctgct acccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa
661 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
721 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
781 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
841 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
901 gcagtcgccc taaaacaaag ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga
961 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
1021 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
1081 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
1141 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
1201 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
1261 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
1321 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
1381 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
1441 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
1501 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
1561 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
1621 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
1681 actacgtgaa aggcgagatc accaaggtag tcggcaaata accctcgagc cacccatgac
1741 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga
1801 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
1861 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
1921 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
1981 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
2041 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
2101 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
2161 tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca
2221 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
2281 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
2341 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
2401 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
2461 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
2521 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
2581 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
2641 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta
2701 ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg
2761 cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg
2821 cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga
2881 caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg
2941 cctggcagtt ccctactctc gcgttaacgc tagcatggat gttttcccag tcacgacgtt
3001 gtaaaacgac ggccagtctt aag
//
