质粒名称: | pCE-hOCT3/4 |
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出品公司: | 爱迪基因 |
目录编号: | 41813 |
存储实验室: | Shinya Yamanaka |
载体骨架基本信息 |
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载体名称: | pCE |
载体出品公司: | -- |
载体抗性: | 氨苄青霉素 |
空载体大小: | 9364 bp |
完整质粒大小: | -- |
载体修饰: | -- |
载体类型: | 哺乳细胞表达 |
筛选标记: | -- |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | POU5F1 |
物种来源: | H. sapiens (human) |
插入大小: | 1080 bp |
基因突变: | -- |
GenBank编号 | NM_002701 |
启动子 | CAG |
标签/融合蛋白: | -- |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | EcoRI (未破坏) |
3' 克隆位点: | EcoRI (未破坏) |
5' 测序引物 | pCAG-F |
3' 测序引物 | TTAGCCAGAAGTCAGATGCTC |
文献引用方法 |
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材料和方法部分: | pCE-hOCT3/4 was a gift from Shinya Yamanaka (Addgene plasmid # 41813 ; http://n2t.net/addgene:41813 ; RRID:Addgene_41813) |
参考文献部分: | An Efficient Non-viral Method to Generate Integration-Free Human iPS Cells from Cord Blood and Peripheral Blood Cells. Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, Goshima N, Yamanaka S. Stem Cells. 2012 Nov 29. doi: 10.1002/stem.1293. 10.1002/stem.1293 PubMed 23193063 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCE-hOCT3/4 10472 bp ds-DNA circular SYN 06-AUG-2018 DEFINITION Non-integrating (episomal) expression of human OCT3/4. ACCESSION . VERSION . KEYWORDS pCE-hOCT3/4 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10472) AUTHORS Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, Goshima N, Yamanaka S TITLE An Efficient Non-viral Method to Generate Integration-Free Human iPS Cells from Cord Blood and Peripheral Blood Cells. JOURNAL Stem Cells. 2012 Nov 29. doi: 10.1002/stem.1293. PUBMED 23193063 REFERENCE 2 (bases 1 to 10472) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10472 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(83..102) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" polyA_site 148..203 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal" primer_bind complement(202..221) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" primer_bind complement(564..580) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(564..580) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(577..599) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 588..604 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(612..642) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 657..678 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." CDS 1157..3082 /codon_start=1 /product="Epstein-Barr nuclear antigen 1, also known as EBNA-1" /label=EBNA1 /note="crucial for latent viral infection, and for episomal amplification of vectors containing the oriP origin (Okita et al., 2013)" /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE GEEGQE" rep_origin 3384..5173 /label=oriP /note="Epstein-Barr virus oriP replication origin (Yates et al., 2000)" primer_bind complement(5715..5732) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5886..6474) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5966..5985) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(6645..7505) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 7268..7287 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(7506..7610) /gene="bla" /label=AmpR promoter enhancer 7641..8020 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 8022..8298 /label=chicken beta-actin promoter intron 8299..9315 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" primer_bind 9239..9261 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 9323..9342 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" ORIGIN 1 aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc 61 tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga 121 agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt 181 gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac 241 atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga 301 acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct 361 tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat 421 ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc 481 tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc 541 agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 601 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 661 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 721 tcgtgccagc ggatctcaat tctcatgttt gacagcttat catcgataag ctgatcctca 781 caggccgcac ccagcttttc ttccgttgcc ccagtagcat ctctgtctgg tgaccttgaa 841 gaggaagagg aggggtcccg agaatcccca tccctaccgt ccagcaaaaa gggggacgag 901 gaatttgagg cctggcttga ggctcaggac gcaaatcttg aggatgttca gcgggagttt 961 tccgggctgc gagtaattgg tgatgaggac gaggatggtt cggaggatgg ggaattttca 1021 gacctggatc tgtctgacag cgaccatgaa ggggatgagg gtgggggggc tgttggaggg 1081 ggcaggagtc tgcactccct gtattcactg agcgtcgtct aataaagatg tctattgatc 1141 tcttttagtg tgaatcatgt ctgacgaggg gccaggtaca ggacctggaa atggcctagg 1201 agagaaggga gacacatctg gaccagaagg ctccggcggc agtggacctc aaagaagagg 1261 gggtgataac catggacgag gacggggaag aggacgagga cgaggaggcg gaagaccagg 1321 agccccgggc ggctcaggat cagggccaag acatagagat ggtgtccgga gaccccaaaa 1381 acgtccaagt tgcattggct gcaaagggac ccacggtgga acaggagcag gagcaggagc 1441 gggaggggca ggagcaggag gggcaggagc aggaggaggg gcaggagcag gaggaggggc 1501 aggaggggca ggaggggcag gaggggcagg agcaggagga ggggcaggag caggaggagg 1561 ggcaggaggg gcaggagggg caggagcagg aggaggggca ggagcaggag gaggggcagg 1621 aggggcagga gcaggaggag gggcaggagg ggcaggaggg gcaggagcag gaggaggggc 1681 aggagcagga ggaggggcag gaggggcagg agcaggagga ggggcaggag gggcaggagg 1741 ggcaggagca ggaggagggg caggagcagg aggggcagga ggggcaggag gggcaggagc 1801 aggaggggca ggagcaggag gaggggcagg aggggcagga ggggcaggag caggaggggc 1861 aggagcagga ggggcaggag caggaggggc aggagcagga ggggcaggag gggcaggagc 1921 aggaggggca ggaggggcag gagcaggagg ggcaggaggg gcaggagcag gaggaggggc 1981 aggaggggca ggagcaggag gaggggcagg aggggcagga gcaggagggg caggaggggc 2041 aggagcagga ggggcaggag gggcaggagc aggaggggca ggaggggcag gagcaggagg 2101 aggggcagga gcaggagggg caggagcagg aggtggaggc cggggtcgag gaggcagtgg 2161 aggccggggt cgaggaggta gtggaggccg gggtcgagga ggtagtggag gccgccgggg 2221 tagaggacgt gaaagagcca gggggggaag tcgtgaaaga gccaggggga gaggtcgtgg 2281 acgtggagaa aagaggccca ggagtcccag tagtcagtca tcatcatccg ggtctccacc 2341 gcgcaggccc cctccaggta gaaggccatt tttccaccct gtaggggaag ccgattattt 2401 tgaataccac caagaaggtg gcccagatgg tgagcctgac gtgcccccgg gagcgataga 2461 gcagggcccc gcagatgacc caggagaagg cccaagcact ggaccccggg gtcagggtga 2521 tggaggcagg cgcaaaaaag gagggtggtt tggaaagcat cgtggtcaag gaggttccaa 2581 cccgaaattt gagaacattg cagaaggttt aagagctctc ctggctagga gtcacgtaga 2641 aaggactacc gacgaaggaa cttgggtcgc cggtgtgttc gtatatggag gtagtaagac 2701 ctccctttac aacctaaggc gaggaactgc ccttgctatt ccacaatgtc gtcttacacc 2761 attgagtcgt ctcccctttg gaatggcccc tggacccggc ccacaacctg gcccgctaag 2821 ggagtccatt gtctgttatt tcatggtctt tttacaaact catatatttg ctgaggtttt 2881 gaaggatgcg attaaggacc ttgttatgac aaagcccgct cctacctgca atatcagggt 2941 gactgtgtgc agctttgacg atggagtaga tttgcctccc tggtttccac ctatggtgga 3001 aggggctgcc gcggagggtg atgacggaga tgacggagat gaaggaggtg atggagatga 3061 gggtgaggaa gggcaggagt gatgtaactt gttaggagac gccctcaatc gtattaaaag 3121 ccgtgtattc ccccgcacta aagaataaat ccccagtaga catcatgcgt gctgttggtg 3181 tatttctggc catctgtctt gtcaccattt tcgtcctccc aacatggggc aattgggcat 3241 acccatgttg tcacgtcact cagctccgcg ctcaacacct tctcgcgttg gaaaacatta 3301 gcgacattta cctggtgagc aatcagacat gcgacggctt tagcctggcc tccttaaatt 3361 cacctaagaa tgggagcaac cagcaggaaa aggacaagca gcgaaaattc acgccccctt 3421 gggaggtggc ggcatatgca aaggatagca ctcccactct actactgggt atcatatgct 3481 gactgtatat gcatgaggat agcatatgct acccggatac agattaggat agcatatact 3541 acccagatat agattaggat agcatatgct acccagatat agattaggat agcctatgct 3601 acccagatat aaattaggat agcatatact acccagatat agattaggat agcatatgct 3661 acccagatat agattaggat agcctatgct acccagatat agattaggat agcatatgct 3721 acccagatat agattaggat agcatatgct atccagatat ttgggtagta tatgctaccc 3781 agatataaat taggatagca tatactaccc taatctctat taggatagca tatgctaccc 3841 ggatacagat taggatagca tatactaccc agatatagat taggatagca tatgctaccc 3901 agatatagat taggatagcc tatgctaccc agatataaat taggatagca tatactaccc 3961 agatatagat taggatagca tatgctaccc agatatagat taggatagcc tatgctaccc 4021 agatatagat taggatagca tatgctatcc agatatttgg gtagtatatg ctacccatgg 4081 caacattagc ccaccgtgct ctcagcgacc tcgtgaatat gaggaccaac aaccctgtgc 4141 ttggcgctca ggcgcaagtg tgtgtaattt gtcctccaga tcgcagcaat cgcgccccta 4201 tcttggcccg cccacctact tatgcaggta ttccccgggg tgccattagt ggttttgtgg 4261 gcaagtggtt tgaccgcagt ggttagcggg gttacaatca gccaagttat tacaccctta 4321 ttttacagtc caaaaccgca gggcggcgtg tgggggctga cgcgtgcccc cactccacaa 4381 tttcaaaaaa aagagtggcc acttgtcttt gtttatgggc cccattggcg tggagccccg 4441 tttaattttc gggggtgtta gagacaacca gtggagtccg ctgctgtcgg cgtccactct 4501 ctttcccctt gttacaaata gagtgtaaca acatggttca cctgtcttgg tccctgcctg 4561 ggacacatct taataacccc agtatcatat tgcactagga ttatgtgttg cccatagcca 4621 taaattcgtg tgagatggac atccagtctt tacggcttgt ccccacccca tggatttcta 4681 ttgttaaaga tattcagaat gtttcattcc tacactagta tttattgccc aaggggtttg 4741 tgagggttat attggtgtca tagcacaatg ccaccactga accccccgtc caaattttat 4801 tctgggggcg tcacctgaaa ccttgttttc gagcacctca catacacctt actgttcaca 4861 actcagcagt tattctatta gctaaacgaa ggagaatgaa gaagcaggcg aagattcagg 4921 agagttcact gcccgctcct tgatcttcag ccactgccct tgtgactaaa atggttcact 4981 accctcgtgg aatcctgacc ccatgtaaat aaaaccgtga cagctcatgg ggtgggagat 5041 atcgctgttc cttaggaccc ttttactaac cctaattcga tagcatatgc ttcccgttgg 5101 gtaacatatg ctattgaatt agggttagtc tggatagtat atactactac ccgggaagca 5161 tatgctaccc gtttagggtt aacaaggggg ccttataaac actattgcta atgccctctt 5221 gagggtccgc ttatcggtag ctacacaggc ccctctgatt gacgttggtg tagcctcccg 5281 tagtcttcct gggcccctgg gaggtacatg tcccccagca ttggtgtaag agcttcagcc 5341 aagagttaca cataaaggca atgttgtgtt gcagtccaca gactgcaaag tctgctccag 5401 gatgaaagcc actcagtgtt ggcaaatgtg cacatccatt tataaggatg tcaactacag 5461 tcagagaacc cctttgtgtt tggtcccccc ccgtgtcaca tgtggaacag ggcccagttg 5521 gcaagttgta ccaaccaact gaagggatta catgcactgc cccgcgaaga aggggcagag 5581 atgtcgtagt caggtttagt tcgtccgggg cggggcatcg atcctctaga gtcgacgcta 5641 gcggatccgc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 5701 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 5761 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 5821 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 5881 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 5941 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 6001 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 6061 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 6121 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 6181 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 6241 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 6301 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 6361 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 6421 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 6481 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 6541 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 6601 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 6661 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 6721 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 6781 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 6841 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 6901 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 6961 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 7021 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 7081 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 7141 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 7201 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 7261 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 7321 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 7381 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 7441 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 7501 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 7561 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 7621 cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt aatcaattac 7681 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg 7741 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc 7801 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac 7861 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa 7921 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac 7981 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt 8041 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt 8101 ttaattattt tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc caggcggggc 8161 ggggcggggc gaggggcggg gcgggcgagg cggagaggtg cggcggcagc caatcagagc 8221 ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag 8281 cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc 8341 gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg 8401 cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg gcttgtttct 8461 tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg cggggggagc 8521 ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg 8581 cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc 8641 gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa 8701 ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg 8761 ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc ttcgggtgcg 8821 gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg 8881 ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga ggggcgcggc 8941 ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg 9001 taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct 9061 gggaggcgcc gccgcacccc ctctagcggg cgcggggcga agcggtgcgg cgccggcagg 9121 aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc 9181 cagcctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg cagggcgggg 9241 ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt tcatgccttc 9301 ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc 9361 aaagaattcg cccttcacca tggcgggaca cctggcttcg gatttcgcct tctcgccccc 9421 tccaggtggt ggaggtgatg ggccaggggg gccggagccg ggctgggttg atcctcggac 9481 ctggctaagc ttccaaggcc ctcctggagg gccaggaatc gggccggggg ttgggccagg 9541 ctctgaggtg tgggggattc ccccatgccc cccgccgtat gagttctgtg gggggatggc 9601 gtactgtggg ccccaggttg gagtggggct agtgccccaa ggcggcttgg agacctctca 9661 gcctgagggc gaagcaggag tcggggtgga gagcaactcc gatggggcct ccccggagcc 9721 ctgcaccgtc acccctggtg ccgtgaagct ggagaaggag aagctggagc aaaacccgga 9781 ggagtcccag gacatcaaag ctctgcagaa agaactcgag caatttgcca agctcctgaa 9841 gcagaagagg atcaccctgg gatatacaca ggccgatgtg gggctcaccc tgggggttct 9901 atttgggaag gtattcagcc aaacgaccat ctgccgcttt gaggctctgc agcttagctt 9961 caagaacatg tgtaagctgc ggcccttgct gcagaagtgg gtggaggaag ctgacaacaa 10021 tgaaaatctt caggagatat gcaaagcaga aaccctcgtg caggcccgaa agagaaagcg 10081 aaccagtatc gagaaccgag tgagaggcaa cctggagaat ttgttcctgc agtgcccgaa 10141 acccacactg cagcagatca gccacatcgc ccagcagctt gggctcgaga aggatgtggt 10201 ccgagtgtgg ttctgtaacc ggcgccagaa gggcaagcga tcaagcagcg actatgcaca 10261 acgagaggat tttgaggctg ctgggtctcc tttctcaggg ggaccagtgt cctttcctct 10321 ggccccaggg ccccattttg gtaccccagg ctatgggagc cctcacttca ctgcactgta 10381 ctcctcggtc cctttccctg agggggaagc ctttccccct gtctctgtca ccactctggg 10441 ctctcccatg cattcaaact gaggtaaggg cg //
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