| 出品公司: | -- |
|---|---|
| 载体名称: | pLac-EGFP |
| 质粒类型: | 大肠杆菌载体；蛋白表达质粒 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | -- |
| 复制子: | pUC |
| 载体大小: | 4261 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 卡那霉素 |
| 筛选标记: | -- |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 大肠杆菌 |
| 备注: | pLac-EGFP是一个大肠杆菌绿色荧光表达质粒 |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
pLacEGFP载体基本信息
pLacEGFP载体图谱质粒图谱和多克隆位点信息
pLacEGFP载体简介
pLacEGFP载体序列
LOCUS pLac-EGFP 4261 bp ds-DNA circular SYN 07-JAN-2018
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..4261
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 33..54
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 69..99
/label=lac promoter
/note="promoter for the E. coli lac operon"
CDS 207..926
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
polyA_signal 1049..1170
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(1177..1632)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1659..1763
/gene="bla"
/label=AmpR promoter
promoter 1765..2122
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 1973..2108
/label=SV40 ori
/note="SV40 origin of replication"
CDS 2157..2951
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=Kan
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3183..3230
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 3559..4147
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattac tagcgctacc ggactcagat cttaatgtga gttagctcac tcattaggca
61 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaatgtc tcatgagcgg
121 atactttcac acaggaaaca gctatggtga gcaagggcga ggagcgtcac cgggtaccgc
181 gggcccggga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
241 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
301 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
361 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
421 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
481 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
541 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
601 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
661 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
721 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
781 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
841 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
901 tcggcatgga cgagctgtac aagtaaagcg gccgcgactc tagatcataa tcagccatac
961 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
1021 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
1081 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
1141 tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt
1201 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
1261 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
1321 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
1381 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
1441 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
1501 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
1561 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
1621 tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
1681 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
1741 aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca
1801 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
1861 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
1921 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
1981 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
2041 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
2101 tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga
2161 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
2221 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
2281 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag
2341 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
2401 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
2461 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
2521 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
2581 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
2641 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg
2701 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
2761 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
2821 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
2881 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
2941 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
3001 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
3061 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
3121 ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
3181 gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg
3241 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata
3301 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct
3361 cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta
3421 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
3481 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
3541 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
3601 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
3661 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
3721 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
3781 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
3841 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
3901 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
3961 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
4021 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
4081 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
4141 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
4201 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca
4261 t
//
pLacEGFP载体图谱质粒图谱pdf版和相关资料下载
pLacEGFP载体质粒应用举例
- 上一篇：pET22b-EGFP
- 下一篇：pET28a-DsRed2
