| 出品公司: | Clontech |
|---|---|
| 载体名称: | pE2F-TA-Luc |
| 质粒类型: | 信号通路报告系统载体 |
| 高拷贝/低拷贝: | -- |
| 启动子: | -- |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 4.9kb |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | Ampicillin |
| 筛选标记: | -- |
| 备注: | -- |
| 产品目录号: | 631914 |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | -- |
买家导航
pE2F-TA-Luc载体质粒基本信息
pE2F-TA-Luc质粒图谱载体图谱和pE2F-TA-Luc载体序列质粒序列多克隆位点信息
pE2F-TA-Luc质粒载体简介
pE2F-TA-Luc is designed to monitor the induction of E2F-mediated signal transduction pathways.
E2F, a major target of the retinoblastoma gene (Rb), is a key regulator of cell-cycle checkpoints
in mammalian cells (1–2). E2F plays a critical role in stimulating expression of genes encoding
growth-promoting proteins (3), and is involved in regulating the expression of important genes
during cell proliferation (4–5). The E2F protein forms a heterodimer complex with the DP1
protein, which binds to E2F response elements and initiates transcription of genes necessary
for DNA replication. Studies have shown that deregulation of E2F results in a loss of cell-cycle
checkpoints—thereby predisposing cells to uncontrolled growth (1–5). pE2F-TA-Luc contains
four copies of the E2F enhancer element (6), located upstream of the minimal TA promoter, the
TATA box from the herpes simplex virus thymidine kinase promoter (PTA). Located downstream
of PTA is the firefly luciferase reporter gene (luc). Upon binding of the E2F/DP1 complex to the
cis-acting E2F enhancer element, transcription is induced and the reporter gene is activated.
The luciferase coding sequence is followed by the SV40 late polyadenylation signal to ensure
proper, efficient processing of the luciferase transcript in eukaryotic cells. A synthetic transcription
blocker (TB) is located upstream of the cis-acting enhancer element. It is composed of
adjacent polyadenylation and transcription pause sites for blocking nonspecific transcription
(7). The vector backbone also contains an f1 origin for single-stranded DNA production, a pUC
origin of replication, and an ampicillin resistance gene for propagation and selection in E. coli.
