| 质粒名称: | pGM1202 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 69615 |
| 存储实验室: | Günther Muth |
|
载体骨架基本信息 |
|
| 载体名称: | pSG5 |
| 载体出品公司: | -- |
| 空载体大小: | 2140 bp |
| 完整质粒大小: | 7002 bp |
| 载体修饰: | -- |
| 载体类型: | -- |
| 筛选标记: | -- |
| 载体抗性: | 阿普霉素，25μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | tsr |
| 物种来源: | 天青链霉菌 |
| 插入大小: | 809 bp |
| 基因突变: | |
| GenBank编号 | X02392 |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pGM1202 was a gift from Günther Muth (Addgene plasmid # 69615 ; http://n2t.net/addgene:69615 ; RRID:Addgene_69615) |
| 参考文献部分: | |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pGM1202 7002 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION inducible gene expression in Streptomyces, C-terminal His-tag
encoding sequence.
ACCESSION .
VERSION .
KEYWORDS pGM1202
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7002)
TITLE pGM190
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7002)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7002
/organism="synthetic DNA construct"
/mol_type="other DNA"
oriT 468..577
/note="incP origin of transfer"
CDS 893..1693
/codon_start=1
/gene="aac(3)IV"
/product="aminoglycoside 3-N-acetyltransferase type IV
"
/label=ApmR
/note="confers resistance to apramycin (Paget and Davies,
1996)"
/translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDSFWRLPNV
KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
LAELLAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
rep_origin 1995..2583
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2484..2503
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 2737..2754
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 2871..2892
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2907..2937
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2945..2961
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2950..2972
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 2969..2985
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 2969..2985
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
terminator 3163..3197
/label=lambda t0 terminator
/note="minimal transcription terminator from phage lambda
(Scholtissek and Grosse, 1987)"
CDS 3392..3409
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
terminator 3601..3649
/label=fd terminator
/note="central terminator from bacteriophage fd (Otsuka and
Kunisawa, 1982)"
CDS 5365..6807
/codon_start=1
/gene="rep"
/product="replication initiator protein from the
Streptomyces ghanaensis plasmid pSG5"
/label=pSG5 Rep
/translation="MWKPGEATWGNTCRCNNVHTCPWCMSRILAVRGSNVQLAADGLAD
AGYGLHLGTNTLRHFERMAFGTVRKGMRHGLVAVLHDGWKGAYGSSGRRWRTMRDDFGI
IGYERAFEDTFGWGSGWHLHWHTLWVTREVLGPDAQAAFRDALAGAWAAGVESAGGYTV
SETCDRPGCSCEGKGHGTDVRPLNGADAADGDAGKQARYLYKDGDKTKGGVAKIGLELA
GQNFKAGRGDDRMGPLDLGDAAAAELQRLRRPGPFVEKYREREFGVFQVRKHYRSQNLN
RLIKELGIQQDVRTEEEITDDTEGLVAIAVIPAYIWYRYIARVAGRRLDLIKVAETYGL
PGVRRLVESWGLVWGKDVLDPPAPEAPAAPGDLDADQMRFEVMSEEEAAFREARRKANE
ARTEELAASLDRVRQPKKEAIRPTISLRKRLKPKPVTVDVKTPPPGAASPVCRRCKGKL
APVLQPWGRLPGDCLRVDTAVA"
ORIGIN
1 aggccgtcga cgacgacgcg tccgcctgcc tgcttttggc cggcgggccg ggccgcggtt
61 gtccgccggg tcggaccggt cccttggtcg accgatgcct cggccacgac gatgctgggc
121 ccgaagcggt acgcgaaccc gtgccgggct gcggcggatc gcggcagcag gagctccccg
181 cccgccacgg ctggctggat ggtcgcctca cgcgcccgca ggggtgacgg cgtgggtgcg
241 gcggtcagcc ccgtgatcgg gcgtcagacg gccgctgagg gaccgcgacc acccctccct
301 tcccgcctgc cgtctttcac cgcccggccg gtccgcagac gccgtacggc caggccacgg
361 aggattacgg gcagatggcg cccacccggc acccgggctg gggcaccctg gacagcacga
421 aggtccgccc cactcaccgg ggcgggcctt cgcgttgagc tagagttccc gccagcctcg
481 cagagcagga ttcccgttga gcaccgccag gtgcgaataa gggacagtga agaaggaaca
541 cccgctcgcg ggtgggccta cttcacctat cctgcccggc tgacgccgtt ggatacacca
601 aggaaagtct acacgaaccc tttggcaaaa tcctgtatat cgtgcgaaaa aggatggata
661 taccgaaaaa atcgctataa tgaccccgaa gcagggttat gcagcggaaa atgcagctca
721 cggtaactga tgccgtattt gcagtaccag cgtacggccc acagaatgat gtcacgctga
781 aaatgccggc ctttgaatgg gttcatgtgc agctccataa gcaaaagggg atgataagtt
841 tatcaccacc gactatttgc aacagtgccg ttgatcgtgc tatgatcgac tgatgtcatc
901 agcggtggag tgcaatgtcg tgcaatacga atggcgaaaa gccgagctca tcggtcagct
961 tctcaacctt ggggttaccc ccggcggtgt gctgctggtc cacagctcct tccgtagcgt
1021 ccggcccctc gaagatgggc cacttggact gatcgaggcc ctgcgtgctg cgctgggtcc
1081 gggagggacg ctcgtcatgc cctcgtggtc aggtctggac gacgagccgt tcgatcctgc
1141 cacgtcgccc gttacaccgg accttggagt tgtctctgac tcattctggc gcctgccaaa
1201 tgtaaagcgc agcgcccatc catttgcctt tgcggcagcg gggccacagg cagagcagat
1261 catctctgat ccattgcccc tgccacctca ctcgcctgca agcccggtcg cccgtgtcca
1321 tgaactcgat gggcaggtac ttctcctcgg cgtgggacac gatgccaaca cgacgctgca
1381 tcttgccgag ttgttggcaa aggttcccta tggggtgccg agacactgca ccattcttca
1441 ggatggcaag ttggtacgcg tcgattatct cgagaatgac cactgctgtg agcgctttgc
1501 cttggcggac aggtggctca aggagaagag ccttcagaag gaaggtccag tcggtcatgc
1561 ctttgctcgg ttgatccgct cccgcgacat tgtggcgaca gccctgggtc aactgggccg
1621 agatccgttg atcttcctgc atccgccaga ggcgggatgc gaagaatgcg atgccgctcg
1681 ccagtcgatt ggctgagctc ataagttcct attccgaagt tcctattcga aatgaccgac
1741 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
1801 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
1861 atgctggagt tcttcgccca ccccaaaagg atctaggtga agatcctttt tgataatctc
1921 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
1981 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
2041 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
2101 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
2161 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
2221 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
2281 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
2341 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc
2401 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
2461 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
2521 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
2581 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
2641 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
2701 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
2761 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
2821 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
2881 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
2941 ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat tacgaattgt
3001 acgtaggcgt cagctacgat gttccgggga ctgctgatcc ggtcagcagg tggaagaggg
3061 actggattcc aaagttctca atgctgcttg ctgttcttga atggggggtc gttgacgacg
3121 acatggctcg attggcgcga caagttgctg cgattctcac caataaaaaa cgcccggcgg
3181 caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg accggatcgg
3241 ggatctgggc tgagggagcc gacggcacgc ggcggctcac ggcgtggcac gcggaacgtc
3301 cgggcttgca cctcacgtca cgtgaggagg cagcgtggac ggcgtcagag aagggagcgg
3361 acatatgaaa tctcccgccc tggccggatc ccatcatcat catcatcatt gactgcagcc
3421 aagcttagat ccccgggtac cgagctcgaa ttccccagat ctaaagtttt gtcgtctttc
3481 cagacgttag taaatgaatt ttctgtatga ggttttgcta aacaactttc aacagtttca
3541 gcggagtgag aatagaaagg aacaactaaa ggaattgcga ataataattt tttcacgttg
3601 aaaatctcca aaaaaaaagg ctccaaaagg agcctttaat tgtatcggtt tatcagcttg
3661 ctttcgaggt gaatttctta aacagcttga taccgatagt tgcgccgaca atgacaacaa
3721 ccatcgccca cgcataaccg atatattcgg tcgctgaggc ttgcagggag tcaaaggccg
3781 cttttgcggg atctcgtcga aggcactaga aggcctatat gcggggatcg accgcgcggg
3841 tcccggacgg ggaagagcgg ggagctttgc cagagagcga cgacttcccc ttgcgttggt
3901 gattgccggt cagggcagcc atccgccatc gtcgcgtagg gtgtcacacc ccaggaatcg
3961 cgtcactgaa cacagcagcc ggtaggacga ccatgactga gttggacacc atcgcaaatc
4021 cgtccgatcc cgcggtgcag cggatcatcg atgtcaccaa gccgtcgcga tccaacataa
4081 agacaacgtt gatcgaggac gtcgagcccc tcatgcacag catcgcggcc ggggtggagt
4141 tcatcgaggt ctacggcagc gacagcagtc cttttccatc tgagttgctg gatctgtgcg
4201 ggcggcagaa cataccggtc cgcctcatcg actcctcgat cgtcaaccag ttgttcaagg
4261 gggagcggaa ggccaagaca ttcggcatcg cccgcgtccc tcgcccggcc aggttcggcg
4321 atatcgcgag ccggcgtggg gacgtcgtcg ttctcgacgg ggtgaagatc gtcgggaaca
4381 tcggcgcgat agtacgcacg tcgctcgcgc tcggagcgtc ggggatcatc ctggtcgaca
4441 gtgacatcac cagcatcgcg gaccggcgtc tccaaagggc cagccgaggt tacgtcttct
4501 cccttcccgt cgttctctcc ggtcgcgagg aggccatcgc cttcattcgg gacagcggta
4561 tgcagctgat gacgctcaag gcggatggcg acatttccgt gaaggaactc ggggacaatc
4621 cggatcggct ggccttgctg ttcggcagcg aaaagggtgg gccttccgac ctgttcgagg
4681 aggcgtcttc cgcctcggtt tccatcccca tgatgagcca gaccgagtct ctcaacgttt
4741 ccgtttccct cggaatcgcg ctgcacgaga ggatcgacag gaatctcgcg gccaaccgat
4801 aagcgcctct gttcctcgga cgctcggttg ctcgacctcg attcgtcagt gatgatcaac
4861 aggacccaga aatggcacga gcccgggatt ggcctcccgg gctcgccgcc cccgaaccgg
4921 cgcagcatgc cgagtgagga cgtttgctct gtgacgagcg tacgagagaa cagcgcgtcg
4981 acaccaccgc gccccgccgg accgaagtgc cactgcggca ccccgatcgt ccacatcccc
5041 gggaagcgcc ccaaaatcta ctgctcggac gcctgccgaa agcgcgcaaa gcgtctcatt
5101 gcggaccagt cacgcagcgt agcaagcacg actacagcgg gtaatccgga cgcgggcttt
5161 agtagtaagg gaaaatctcc gtccactgca ggtcagagcg ctgtaactgg aacgggttct
5221 accccaaacg agggtgccgc gacagccggg cgggaggacc cgcgcgacga gcggttcgcc
5281 cggcgggacc ggcaccagac ggtgtcgctg aacgaggcgt tcaagggctg cgggacccgg
5341 ctcactgccg ggacggccga gctgatgtgg aagccgggcg aggcgacgtg gggcaacacg
5401 tgccgctgca acaacgtcca cacctgcccg tggtgcatga gccgcatcct ggccgtacgg
5461 ggctcgaacg tgcagctcgc cgccgacggt ctcgcggacg ccgggtacgg gctgcacctt
5521 ggcacgaaca cgttgcgcca cttcgagcgc atggcgttcg gcaccgtccg caagggaatg
5581 cggcacggcc tggtcgcggt cttgcacgac ggctggaagg gcgcgtacgg ctcgtccggc
5641 cgccgctggc gcacgatgcg cgacgacttc gggatcatcg gctacgagcg ggccttcgag
5701 gacaccttcg gctggggctc cggctggcac ctgcactggc acacgctctg ggtcacccgc
5761 gaggtgctcg gcccggacgc ccaggccgcg ttccgcgacg cactcgccgg agcctgggcc
5821 gccggggtcg aatccgcggg cggctacacc gtcagcgaga cgtgcgaccg gcccggttgc
5881 tcctgtgagg gcaagggcca cggcaccgac gtgcgcccac tcaacggcgc ggacgccgct
5941 gacggcgatg cgggcaagca ggcccgctac ctctacaagg acggcgacaa gaccaagggc
6001 ggcgtcgcca agatcggcct ggagctggcc ggacagaact tcaaggccgg tcgcggcgac
6061 gaccgcatgg gaccgctcga cctgggcgac gcggcggccg ccgagctgca gcggcttcgt
6121 cggcccggcc cgttcgtcga gaagtaccgc gagcgcgagt tcggcgtctt ccaggtccgc
6181 aagcactacc ggtcgcagaa cctgaaccgg ctgatcaagg aactcggcat ccagcaggac
6241 gtccgcaccg aggaggagat caccgacgac accgagggcc tggtcgccat cgcggtcatc
6301 ccggcctaca tctggtaccg atacatcgcc cgcgtcgccg gacgtcgcct cgacctcatc
6361 aaggtcgccg agacgtacgg cctaccgggc gtccggcggc tcgtcgagtc ctggggcctg
6421 gtgtggggca aggacgtcct ggacccgccc gccccggaag cgccggccgc gccgggcgac
6481 cttgacgctg accagatgag gttcgaggtc atgtccgagg aggaagctgc tttccgcgag
6541 gcgcgccgga aggccaacga ggcccgcacg gaggagctgg ccgcttcgct cgaccgagtg
6601 cgtcagccga agaaggaggc gatccgcccg acgatcagcc ttcggaagcg cctgaagccg
6661 aagccggtca ccgtcgacgt caagactccc ccgcccggcg ccgcgtcgcc cgtgtgccgc
6721 cgctgcaagg gcaagctcgc cccggtactc cagccctggg gccggctccc cggagactgc
6781 ctccgcgtcg acacagcggt cgcgtagggc cgctgaggga ccgccatgag accgccaccc
6841 cggacatccg ggtggcggtc tcatggcggt cccggcgcga aagccgaggg tctgcctgcc
6901 gtgaggtggc cggcggacct gggttactgc tggaatcccg cgcccagtcc gagcgtccgc
6961 cggcgctttt cacctggctc cgggtgctcg tcgatgagca cc
//
