| 质粒名称: | p11L1w |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 37330 |
| 存储实验室: | John Schiller |
|
载体骨架基本信息 |
|
| 载体名称: | custom |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | 6187 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达 |
| 筛选标记: | -- |
| 载体抗性: | 卡那霉素，50μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
| 拷贝数: | -- |
|
插入基因信息 |
|
| 插入基因名称: | HPV11 L1 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | EF1a |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | p11L1w was a gift from John Schiller (Addgene plasmid # 37330 ; http://n2t.net/addgene:37330 ; RRID:Addgene_37330) |
| 参考文献部分: | Codon optimization of the human papillomavirus 11 (HPV 11) L1 gene leads to increased gene expression and formation of virus-like particles in mammalian epithelial cells. Mossadegh N, Gissmann L, Muller M, Zentgraf H, Alonso A, Tomakidi P. Virology. 2004 Aug 15;326(1):57-66. 10.1016/j.virol.2004.04.050 PubMed 15262495 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS p11L1w 6187 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS p11L1w
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6187)
AUTHORS Mossadegh N, Gissmann L, Muller M, Zentgraf H, Alonso A, Tomakidi P
TITLE Codon optimization of the human papillomavirus 11 (HPV 11) L1 gene
leads to increased gene expression and formation of virus-like
particles in mammalian epithelial cells.
JOURNAL Virology. 2004 Aug 15;326(1):57-66.
PUBMED 15262495
REFERENCE 2 (bases 1 to 6187)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6187
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 113..1291
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 344..1282
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 1239..1259
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
primer_bind 1384..1400
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 1384..1400
/label=pBluescriptSK
/note="For pBluescript vector"
misc_feature 3004..3592
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(3057..3077)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3475..3486)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
promoter complement(3614..3631)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
polyA_signal 3649..3770
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3660..3679)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 3714..3733
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
rep_origin 3958..4546
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4447..4466
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind complement(4630..4650)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 4635..4964
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 4815..4950
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4877..4896
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 5012..5059
/label=EM7 promoter
/note="synthetic bacterial promoter "
promoter 5051..5069
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 5079..5873
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(5133..5152)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 5743..5762
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(5931..5950)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 5975..6022
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
1 ccgctaccgg actcagatct cgagctcaag cttgcaaaga tggataaagt tttaaacaga
61 gaggaatctt tgcagctaat ggaccttgta ggtcttgaaa ggagtgggaa ttggctccgg
121 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
181 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
241 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
301 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
361 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
421 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
481 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
541 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
601 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
661 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
721 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
781 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
841 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
901 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
961 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
1021 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
1081 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
1141 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
1201 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
1261 aagttttttt cttccatttc aggtgtcgtg aggaattccg tagagatccc tcgacctcga
1321 gatccatcct gtacggaagt gttacttctg ctctaaaagc tgcggaattg tacccgcggc
1381 cgctctagaa ctagtggatc cactagtaac ggccgccagt gtgctggaat tcgcccttcg
1441 gaattcatgt ggagacccag cgacagcacc gtgtacgtgc ccccccccaa ccccgtgagc
1501 aaggtggtgg ccaccgacgc ctacgtgaag agaaccaaca tcttctacca cgccagcagc
1561 agcagactgc tggccgtggg ccacccctac tacagcatca agaaggtgaa caagaccgtg
1621 gtgcccaagg tgagcggcta ccagtacaga gtgttcaagg tggtgctgcc cgaccccaac
1681 aagttcgccc tgcccgacag cagcctgttc gaccccacca cccagagact ggtgtgggcc
1741 tgcaccggcc tggaggtggg cagaggccag cccctgggcg tgggcgtgag cggccacccc
1801 ctgctgaaca agtacgacga cgtggagaac agcggcggct acggcggcaa ccccggccag
1861 gacaacagag tgaacgtggg catggactac aagcagaccc agctgtgcat ggtgggctgc
1921 gccccccccc tgggcgagca ctggggcaag ggcacccagt gcagcaacac cagcgtgcag
1981 aacggcgact gcccccccct ggagctgatc accagcgtga tccaggacgg cgacatggtg
2041 gacaccggct tcggcgccat gaacttcgcc gacctgcaga ccaacaagag cgacgtgccc
2101 ctggacatct gcggcaccgt gtgcaagtac cccgactacc tgcagatggc cgccgacccc
2161 tacggcgaca gactgttctt ctacctgaga aaggagcaga tgttcgccag acacttcttc
2221 aacagagccg gcaccgtggg cgagcccgtg cccgacgacc tgctggtgaa gggcggcaac
2281 aacagaagca gcgtggccag cagcatctac gtgcacaccc ccagcggcag cctggtgagc
2341 agcgaggccc agctgttcaa caagccctac tggctgcaga aggcccaggg ccacaacaac
2401 ggcatctgct ggggcaacca cctgttcgtg accgtggtgg acaccaccag aagcaccaac
2461 atgaccctgt gcgccagcgt gagcaagagc gccacctaca ccaacagcga ctacaaggag
2521 tacatgagac acgtggagga gttcgacctg cagttcatct tccagctgtg cagcatcacc
2581 ctgagcgccg aggtgatggc ctacatccac accatgaacc ccagcgtgct ggaggactgg
2641 aacttcggcc tgagcccccc ccccaacggc accctggagg acacctacag atacgtgcag
2701 agccaggcca tcacctgcca gaagcccacc cccgagaagg agaagcagga cccctacaag
2761 gacatgagct tctgggaggt gaacctgaag gagaagttca gcagcgagct ggaccagttc
2821 cccctgggca gaaagttcct gctgcagagc ggctacagag gcagaaccag cgccagaacc
2881 ggcatcaaga gacccgccgt gagcaagccc agcaccgccc ccaagagaaa gagaaccaag
2941 accaagaagt gaaagctttt tgaattcttt ggatccacta gtgtcgatat caagcttatc
3001 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
3061 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
3121 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
3181 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
3241 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
3301 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
3361 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
3421 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
3481 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
3541 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac
3601 cgtcggccgc ttccctttag tgagggttaa tgcttcgagc agacatgata agatacattg
3661 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
3721 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
3781 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
3841 aaaacctcta caaatgtggt atggaattct taattaacta gccatgacca aaatccctta
3901 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
3961 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
4021 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
4081 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
4141 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
4201 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
4261 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
4321 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
4381 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
4441 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
4501 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
4561 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct taattaagct
4621 gtacactgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
4681 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
4741 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
4801 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
4861 actgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
4921 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
4981 gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat
5041 cggcatagta taatacgact cactatagga gggccaccat gattgaacaa gatggattgc
5101 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
5161 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
5221 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
5281 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
5341 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
5401 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
5461 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
5521 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
5581 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgacac
5641 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
5701 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
5761 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
5821 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
5881 tctggggttc gaaatgaccg accaagcgaa ttcgctagag ggaggctaac tgaaacacgg
5941 aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg
6001 cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc
6061 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc
6121 caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct
6181 gccatag
//
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