| 质粒名称: | pInducer20 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 44012 |
| 存储实验室: | Stephen Elledge |
|
载体骨架基本信息 |
|
| 载体名称: | pHAGE-TRE |
| 载体出品公司: | Elledge |
| 载体抗性: | 氯霉素/氨苄青霉素 |
| 空载体大小: | 11791 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 30 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | HA（主干上的C终端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | LNCX_primer |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pInducer20 was a gift from Stephen Elledge (Addgene plasmid # 44012 ; http://n2t.net/addgene:44012 ; RRID:Addgene_44012) |
| 参考文献部分: | The pINDUCER lentiviral toolkit for inducible RNA interference in vitro and in vivo. Meerbrey KL, Hu G, Kessler JD, Roarty K, Li MZ, Fang JE, Herschkowitz JI, Burrows AE, Ciccia A, Sun T, Schmitt EM, Bernardi RJ, Fu X, Bland CS, Cooper TA, Schiff R, Rosen JM, Westbrook TF, Elledge SJ. Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 10.1073/pnas.1019736108. Epub 2011 Feb 9. 10.1073/pnas.1019736108 PubMed 21307310 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pInducer20 11794 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Tet-inducible lentiviral vector for ORF expression.
ACCESSION .
VERSION .
KEYWORDS pInducer20
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11794)
AUTHORS Meerbrey KL, Hu G, Kessler JD, Roarty K, Li MZ, Fang JE,
Herschkowitz JI, Burrows AE, Ciccia A, Sun T, Schmitt EM, Bernardi
RJ, Fu X, Bland CS, Cooper TA, Schiff R, Rosen JM, Westbrook TF,
Elledge SJ
TITLE The pINDUCER lentiviral toolkit for inducible RNA interference in
vitro and in vivo.
JOURNAL Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi:
10.1073/pnas.1019736108. Epub 2011 Feb 9.
PUBMED 21307310
REFERENCE 2 (bases 1 to 11794)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11794
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1721..1765
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2058..2175
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
protein_bind 2230..2500
/label=tetracycline response element
/bound_moiety="tetracycline repressor TetR"
/note="contains seven copies of the tetracycline operator
tetO"
promoter 2533..2571
/label=minimal CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2568..2592
/label=LNCX
/note="Human CMV promoter, forward primer"
protein_bind 2701..2825
/gene="mutant version of attR"
/label=attR1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
promoter 2850..2880
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
CDS 2934..3593
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind complement(3000..3019)
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
CDS 3935..4240
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 4119..4138
/label=ccdB-fwd
/note="ccdB gene, forward primer"
protein_bind complement(4281..4405)
/gene="mutant version of attR"
/label=attR2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
CDS 4458..4484
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
promoter 4570..5781
/label=UbC promoter
/note="human ubiquitin C promoter"
primer_bind 5618..5637
/label=hUBCpro-F
/note="Human Ubiquitin C (UbC) promoter, forward primer"
CDS 5840..6586
/codon_start=1
/product="improved tetracycline-controlled transactivator"
/label=rtTA-Advanced
/note="In the Tet-On(R) system, rtTA-Advanced binds to the
Tet-responsive element and stimulates transcription only in
the presence of tetracycline or doxycycline."
/translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
DALDDFDLDMLPADALDDFDLDMLPG"
misc_feature 6624..7197
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(6791..6808)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 7018..7037
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 7213..8007
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(7267..7286)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 7877..7896
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
misc_feature 8184..8772
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(8237..8257)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(8655..8666)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 8847..9080
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
primer_bind complement(9199..9217)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 9285..9389
/gene="bla"
/label=AmpR promoter
CDS 9390..10250
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(9608..9627)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 10421..11009
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 10910..10929
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 11163..11180
/label=L4440
/note="L4440 vector, forward primer"
primer_bind 11272..11291
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
protein_bind 11420..11441
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 11456..11486
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 11494..11510
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 11499..11521
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 11518..11534
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 11518..11534
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 11584..11603
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
2401 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
2461 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg
2521 tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
2581 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
2641 gaccgatcca gcctccgcgg ccccgaacta gtccagtgtg gtggaattct gcagatatca
2701 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
2761 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
2821 ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg
2881 tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga
2941 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
3001 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
3061 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
3121 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
3181 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
3241 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
3301 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
3361 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
3421 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
3481 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
3541 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg
3601 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
3661 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca
3721 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
3781 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
3841 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
3901 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
3961 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
4021 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
4081 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
4141 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
4201 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
4261 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
4321 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
4381 gttcagcttt cttgtacaaa gtggttgata tccagcacag tggcggccgc tcgagtctag
4441 agggcccgcg gttcgaatac ccatacgacg tcccagacta cgcttagtaa tgattaatta
4501 aactagaaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agtaacccac
4561 ccaagatctg gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc ctcctcacgg
4621 cgagcgctgc cacgtcagac gaagggcgca gcgagcgtcc tgatccttcc gcccggacgc
4681 tcaggacagc ggcccgctgc tcataagact cggccttaga accccagtat cagcagaagg
4741 acattttagg acgggacttg ggtgactcta gggcactggt tttctttcca gagagcggaa
4801 caggcgagga aaagtagtcc cttctcggcg attctgcgga gggatctccg tggggcggtg
4861 aacgccgatg attatataag gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg
4921 gatttgggtc gcggttcttg tttgtggatc gctgtgatcg tcacttggtg agtagcgggc
4981 tgctgggctg gccggggctt tcgtggccgc cgggccgctc ggtgggacgg aagcgtgtgg
5041 agagaccgcc aagggctgta gtctgggtcc gcgagcaagg ttgccctgaa ctgggggttg
5101 gggggagcgc agcaaaatgg cggctgttcc cgagtcttga atggaagacg cttgtgaggc
5161 gggctgtgag gtcgttgaaa caaggtgggg ggcatggtgg gcggcaagaa cccaaggtct
5221 tgaggccttc gctaatgcgg gaaagctctt attcgggtga gatgggctgg ggcaccatct
5281 ggggaccctg acgtgaagtt tgtcactgac tggagaactc ggtttgtcgt ctgttgcggg
5341 ggcggcagtt atggcggtgc cgttgggcag tgcacccgta cctttgggag cgcgcgccct
5401 cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg cagggtgggg ccacctgccg
5461 gtaggtgtgc ggtaggcttt tctccgtcgc aggacgcagg gttcgggcct agggtaggct
5521 ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg gataagtgag gcgtcagttt
5581 ctttggtcgg ttttatgtac ctatcttctt aagtagctga agctccggtt ttgaactatg
5641 cgctcggggt tggcgagtgt gttttgtgaa gttttttagg caccttttga aatgtaatca
5701 tttgggtcaa tatgtaattt tcagtgttag actagtaaat tgtccgctaa attctggccg
5761 tttttggctt ttttgttaga cgaagcttgg taccgagctc ggatctccac cccgtaccgg
5821 tcctgcagtc gaattcacca tgtctagact ggacaagagc aaagtcataa acggagctct
5881 ggaattactc aatggtgtcg gtatcgaagg cctgacgaca aggaaactcg ctcaaaagct
5941 gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc tgctcgatgc
6001 cctgccaatc gagatgctgg acaggcatca tacccacttc tgccccctgg aaggcgagtc
6061 atggcaagac tttctgcgga acaacgccaa gtcataccgc tgtgctctcc tctcacatcg
6121 cgacggggct aaagtgcatc tcggcacccg cccaacagag aaacagtacg aaaccctgga
6181 aaatcagctc gcgttcctgt gtcagcaagg cttctccctg gagaacgcac tgtacgctct
6241 gtccgccgtg ggccacttta cactgggctg cgtattggag gaacaggagc atcaagtagc
6301 aaaagaggaa agagagacac ctaccaccga ttctatgccc ccacttctga gacaagcaat
6361 tgagctgttc gaccggcagg gagccgaacc tgccttcctt ttcggcctgg aactaatcat
6421 atgtggcctg gagaaacagc taaagtgcga aagcggcggg ccgaccgacg cccttgacga
6481 ttttgactta gacatgctcc cagccgatgc ccttgacgac tttgaccttg atatgctgcc
6541 tgctgacgct cttgacgatt ttgaccttga catgctcccc gggtaactaa gtaaggatcc
6601 gcggccgcac tagaggaatt ccgcccctct cccccccccc cccctaacgt tactggccga
6661 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
6721 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
6781 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
6841 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
6901 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
6961 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
7021 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
7081 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa
7141 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataagct
7201 taccggtcca ccatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
7261 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
7321 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
7381 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
7441 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
7501 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
7561 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
7621 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
7681 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
7741 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
7801 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
7861 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
7921 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
7981 tatcgccttc ttgacgagtt cttctgatgt acaagtaaag cggccgcgac tctagatcat
8041 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
8101 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt tagtccctcc caattcgata
8161 tcaagcttat cgcgatagat cctaatcaac ctctggatta caaaatttgt gaaagattga
8221 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
8281 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
8341 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
8401 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
8461 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
8521 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
8581 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
8641 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
8701 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
8761 ccgcctcccc gcctgagatc ctttaagacc aatgacttac aaggcagctg tagatcttag
8821 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
8881 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
8941 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
9001 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
9061 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
9121 tgcaaagaaa tgaatatcag agagtgagag gcccgggtta attaaggaaa gggctagatc
9181 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
9241 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
9301 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
9361 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
9421 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
9481 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
9541 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
9601 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc
9661 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
9721 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
9781 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
9841 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
9901 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
9961 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
10021 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
10081 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
10141 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
10201 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
10261 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
10321 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
10381 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
10441 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
10501 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
10561 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
10621 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
10681 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
10741 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
10801 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
10861 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
10921 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
10981 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
11041 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
11101 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
11161 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
11221 cgcgttggcc gattcattaa tgcagcaagc tcatggctga ctaatttttt ttatttatgc
11281 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
11341 aggcctaggc ttttgcaaaa agctccccgt ggcacgacag gtttcccgac tggaaagcgg
11401 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
11461 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
11521 gaaacagcta tgacatgatt acgaatttca caaataaagc atttttttca ctgcattcta
11581 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc
11641 aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc
11701 tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt
11761 gtatttgtta aatatgtact acaaacttag tagt
//
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