pFC902

LOCUS      pFC902               16689 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  The vector has a U3 promoter and terminator controlled gene encoding
            a transcript containing the sgRNA flanked by a tRNA-Gly repeat; and 
            a gene encoding cas9 controlled by Ptef1 and Ttef1.
ACCESSION   .
VERSION     .
KEYWORDS    pFC902
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16689)
  AUTHORS   Nodvig CS, Hoof JB, Kogle ME, Jarczynska ZD, Lehmbeck J, Klitgaard 
            DK, Mortensen UH
  TITLE     Efficient Oligo nucleotide mediated CRISPR-Cas9 Gene Editing in 
            Aspergilli.
  JOURNAL   Fungal Genet Biol. 2018 Jan 8. pii: S1087-1845(18)30004-5. doi: 
            10.1016/j.fgb.2018.01.004.
  PUBMED    29325827
REFERENCE   2  (bases 1 to 16689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..16689
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     622..641
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(860..951)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     993..1012
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1018..1037
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    1244..1277
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     rep_origin      1590..6836
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
     misc_RNA        7669..7744
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             8946..13049
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             13053..13073
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(15189..15222)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(15692..15709)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(15692..15708)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15807..15824)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15978..16566)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(16058..16077)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(16688..859)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
       61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
      121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc
      181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
      241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
      301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
      361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
      421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
      481 tggttatggc agcgctacat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
      541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
      601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
      661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt
      901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca
      961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg
     1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg
     1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga
     1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1201 cccgggctaa ttatggggtg tcgcccttat tcgactctat agtgaagttc ctattctcta
     1261 gaaagtatag gaacttctga agtggggatt taaatgcggc cgcgctgagg gtttaatcga
     1321 cgaagcagct gacggccagt gccaagctta acgcgtaccg ggcccagtat atgttccgca
     1381 gatgactgga gctctgccat acgtgccctc tcaagcacca tttgttccat ctacagagac
     1441 tagtcaccaa ctagtctatc aagactcaca gggtacattg ctgagaccaa ctgaccagag
     1501 gcagggtagc ggattgacgg ctccatctcc ttcacttaca aggtctattg aaagcccttt
     1561 agcatcacca agcggagaat agattgttaa gcttattttt tgtatactgt tttgtgatag
     1621 cacgaagttt ttccacggta tcttgttaaa aatatatatt tgtggcgggc ttacctacat
     1681 caaattaata agagactaat tataaactaa acacacaagc aagctacttt agggtaaaag
     1741 tttataaatg cttttgacgt ataaacgttg cttgtattta ttattacaat taaaggtgga
     1801 tagaaaacct agagactagt tagaaactaa tctcaggttt gcgttaaact aaatcagagc
     1861 ccgagaggtt aacagaacct agaaggggac tagatatccg ggtagggaaa caaaaaaaaa
     1921 aacaagacag ccacatatta gggagactag ttagaagcta gttccaggac taggaaaata
     1981 aaagacaatg ataccacagt ctagttgaca actagataga ttctagattg aggccaaagt
     2041 ctctgagatc caggttagtt gcaactaata ctagttagta tctagtctcc tataactctg
     2101 aagctagaat aacttactac tattatcctc accactgttc agctgcgcaa acggagtgat
     2161 tgcaaggtgt tcagagacta gttattgact agtcagtgac tagcaataac taacaaggta
     2221 ttaacctacc atgtctgcca tcaccctgca cttcctcggg ctcagcagcc ttttcctcct
     2281 cattttcatg ctcattttcc ttgtttaaga ctgtgactag tcgaagacta gtccagaacc
     2341 acaaaggaga aatgtcttac cactttcttc attgcttgtc tcttttgcat tatccatgtc
     2401 tgcaactagt tagagtctag ttagtgacta gtccgacgag gacttgcttg tctccggatt
     2461 gttggaggaa ctctccaggg cctcaagatc cacaacagag ccttctagaa gactggtcaa
     2521 taactagttg gtctttgtct gagtctgact tacgaggttg catactcgct ccctttgcct
     2581 cgtcaatcga tgagaaaaag cgccaaaact cgcaatatgg ctttgaacca cacggtgctg
     2641 agactagtta gaatctagtc ccaaactagc ttggatagct tacctttgcc ctttgcgttg
     2701 cgacaggtct tgcagggtat ggttcctttc tcaccagctg atttagctgc cttgctaccc
     2761 tcacggcgga tctgcataaa gagtggctag aggttataaa ttagcactga tcctaggtac
     2821 ggggctgaat gtaacttgcc cttcctttct catcgcgcgg caagacaggc ttgctcaaat
     2881 tcctaccagt cacaggggta tgcacggcgt acggaccact tgaactagtc acagattagt
     2941 tagcaactag tctgcattga atggctgtac ttacgggccc tcgccattgt cctgatcatt
     3001 tccagcttca ccctcgttgc tgcaaagtag ttagtgacta gtcaaggact agttgaaatg
     3061 ggagaagaaa ctcacgaatt ctcgacaccc ttagtattgt ggtccttgga cttggtgctg
     3121 ctatatatta gctaatacac tagttagact cacggaaact tacgcagctc gcttgcgctt
     3181 cttggtagga gtcggggttg ggagaacagt gccttcaaac aagccttcat accatgctac
     3241 ttgactagtc agggactagt caccaagtaa tctagatagg acttgccttt ggcctccatc
     3301 agttccttca tagtgggagg tccattgtgc aatgtaaact ccatgccgtg ggagttcttg
     3361 tccttcaagt gcttgaccaa tatgtttctg ttggcagagg gaacctgtca actagttaat
     3421 aactagtcag aaactagtat agcagtagac tcactgtacg cttgaggcat cccttcactc
     3481 ggcagtagac ttcatatgga tggatatcag gcacgccatt gtcgtcctgt ggactagtca
     3541 gtaactaggc ttaaagctag tcgggtcggc ttactatctt gaaatccggc agcgtaagct
     3601 ccccgtcctt aactgcctcg agatagtgac agtactctgg ggactttcgg agatcgttat
     3661 cgcgaatgct cggcatacta atcgttgact agtcttggac tagtcccgag caaaaaggat
     3721 tggaggagga ggaggaaggt gagagtgaga caaagagcga aataagagct tcaaaggcta
     3781 tctctaagca gtatgaaggt taagtatcta gttcttgact agatttaaaa gagatttcga
     3841 ctagttatgt acctggagtt tggatatagg aatgtgttgt ggtaacgaaa tgtaaggggg
     3901 aggaaagaaa aagtcggtca agaggtaact ctaagtcggc cattcctttt tgggaggcgc
     3961 taaccataaa cggcatggtc gacttagagt tagctcaggg aatttaggga gttatctgcg
     4021 accaccgagg aacggcggaa tgccaaagaa tcccgatgga gctctagctg gcggttgaca
     4081 accccacctt ttggcgtttc tgcggcgttg caggcgggac tggatacttc gtagaaccag
     4141 aaaggcaagg cagaacgcgc tcagcaagag tgttggaagt gatagcatga tgtgccttgt
     4201 taactaggtc aaaatctgca gtatgcttga tgttatccaa agtgtgagag aggaaggtcc
     4261 aaacatacac gattgggaga gggcctaggt ataagagttt ttgagtagaa cgcatgtgag
     4321 cccagccatc tcgaggagat taaacacggg ccggcatttg atggctatgt tagtacccca
     4381 atggaaacgg tgagagtcca gtggtcgcag ataactccct aaattccctg agctaactct
     4441 aagtcgacca tgccgtttat ggttagcgcc tcccaaaaag gaatggccga cttagagtta
     4501 cctcttgacc gactttttct ttcctccccc ttacatttcg ttaccacaac acattcctat
     4561 atccaaactc caggtacata actagtcgaa atctctttta aatctagtca agaactagat
     4621 acttaacctt catactgctt agagatagcc tttgaagctc ttatttcgct ctttgtctca
     4681 ctctcacctt cctcctcctc ctccaatcct ttttgctcgg gactagtcca agactagtca
     4741 acgattagta tgccgagcat tcgcgataac gatctccgaa agtccccaga gtactgtcac
     4801 tatctcgagg cagttaagga cggggagctt acgctgccgg atttcaagat agtaagccga
     4861 cccgactagc tttaagccta gttactgact agtccacagg acgacaatgg cgtgcctgat
     4921 atccatccat atgaagtcta ctgccgagtg aagggatgcc tcaagcgtac agtgagtcta
     4981 ctgctatact agtttctgac tagttattaa ctagttgaca ggttccctct gccaacagaa
     5041 acatattggt caagcacttg aaggacaaga actcccacgg catggagttt acattgcaca
     5101 atggacctcc cactatgaag gaactgatgg aggccaaagg caagtcctat ctagattact
     5161 tggtgactag tccctgacta gtcaagtagc atggtatgaa ggcttgtttg aaggcactgt
     5221 tctcccaacc ccgactccta ccaagaagcg caagcgagct gcgtaagttt ctgtgagtct
     5281 aactagtgta ttagctaata tatagcagca ccaagtccaa ggaccacaat actaagggtg
     5341 tcgagaattc gtgagtttct tctcccattt caactagtcc ttgactagtc actaactact
     5401 ttgcagcaac gagggtgaag ctggaaatga tcaggacaat ggcgagggcc cgtaagtaca
     5461 gccattcaat gcagactagt tgctaactaa tctgtgacta gttcaagtgg tccgtacgcc
     5521 gtgcataccc ctgtgactgg taggaatttg agcaagcctg tcttgccgcg cgatgagaaa
     5581 ggaagggcaa gttacattca gccccgtacc taggatcagt gctaatttat aacctctagc
     5641 cactctttat gcagatccgc cgtgagggta gcaaggcagc taaatcagct ggtgagaaag
     5701 gaaccatacc ctgcaagacc tgtcgcaacg caaagggcaa aggtaagcta tccaagctag
     5761 tttgggacta gattctaact agtctcagca ccgtgtggtt caaagccata ttgcgagttt
     5821 tggcgctttt tctcatcgat tgacgaggca aagggagcga gtatgcaacc tcgtaagtca
     5881 gactcagaca aagaccaact agttattgac cagtcttcta gaaggctctg ttgtggatct
     5941 tgaggccctg gagagttcct ccaacaatcc ggagacaagc aagtcctcgt cggactagtc
     6001 actaactaga ctctaactag ttgcagacat ggataatgca aaagagacaa gcaatgaaga
     6061 aagtggtaag acatttctcc tttgtggttc tggactagtc ttcgactagt cacagtctta
     6121 aacaaggaaa atgagcatga aaatgaggag gaaaaggctg ctgagcccga ggaagtgcag
     6181 ggtgatggca gacatggtag gttaatacct tgttagttat tgctagtcac tgactagtca
     6241 ataactagtc tctgaacacc ttgcaatcac tccgtttgcg cagctgaaca gtggtgagga
     6301 taatagtagt aagttattct agcttcagag ttataggaga ctagatacta actagtatta
     6361 gttgcaacta acctggatct cagagacttt ggcctcaatc tagaatctat ctagttgtca
     6421 actagactgt ggtatcattg tcttttattt tcctagtcct ggaactagct tctaactagt
     6481 ctccctaata tgtggctgtc ttgttttttt ttttgtttcc ctacccggat atctagtccc
     6541 cttctaggtt ctgttaacct ctcgggctct gatttagttt aacgcaaacc tgagattagt
     6601 ttctaactag tctctaggtt ttctatccac ctttaattgt aataataaat acaagcaacg
     6661 tttatacgtc aaaagcattt ataaactttt accctaaagt agcttgcttg tgtgtttagt
     6721 ttataattag tctcttatta atttgatgta ggtaagcccg ccacaaatat atatttttaa
     6781 caagataccg tggaaaaact tcgtgctatc acaaaacagt atacaaaaaa taagcttaac
     6841 aatctattct ccgcttggtg atgctaaagg gctttcaata gaccttgtaa gtgaaggaga
     6901 tggagccgtc aatccgctac cctgcctctg gtcagttggt ctcagcaatg taccctgtga
     6961 gtcttgatag actagttggt gactagtctc tgtagatgga acaaatggtg cttgagaggg
     7021 cacgtatggc agagctccag tcatctgcgg aacatatact gggcccggga agatctcatg
     7081 gtcatagctg tttccgctga gggtttaatg atcacataga tgctcggttg acaggaccac
     7141 ctatagcaag tactttgtag gaggttctcc taacgcttgg cttcaaatcc aatcaggata
     7201 tcagtgagtt actgtttgtc atcgcatcct catgaagtcg ctcaacagcc actgagaagc
     7261 aaatatttgg gagaccatcc ctgatgttga aattttacgc tggagcccat tcaccggtga
     7321 gctcggggac agtctggcca gtggagcgga aaatcttcaa ctaactctga ttggtaccac
     7381 aggtagcccc acagaacaaa ccgagcaaac atgaaaattt tcgcttgagg ttagcgcact
     7441 cgctagcgcc tgcccgcaaa tataaaaggc cccgaaatcc catgcatttt ggaaatcaag
     7501 cgactctacg tatgtcatcc ccctccctac ctttgggaaa acctctcaac tgcaagtcag
     7561 aacattttgc taacagcgca tcattggtct agtggtagaa ttcatcgttg ccatcgatga
     7621 ggcccgtgtt cgattcacgg atgatgcagg cggagtatca taacatcggt tttagagcta
     7681 gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg
     7741 gtgcttttgc atcattggtc tagtggtaga attcatcgtt gccatcgatg aggcccgtgt
     7801 tcgattcacg gatgatgcat gtcaattttt acacttgatg ttggtgtaat caggcgatca
     7861 gagtttgctc aaattctctc ttgttttaga ttctgaagaa tataattttt acatgactag
     7921 ttcattttac ttccaagtag actatttgta gattgagatt gccgtcaact ttattcatag
     7981 aatcacatta aattgctcat tgacctgcat gtctatgcac acattcacat ctatcttctc
     8041 agggtattaa gacctcagcc gagacagcag aatcaccgcc caagttaagc ctttgtgctg
     8101 atcatgctct cgaacgggcc aagttcggga aaagcaaagg agcgtttagt gaggggcaat
     8161 ttgactcacc tcccaggcaa cagatgaggg gggcaaaaag aaagaaattt tcgtgagtca
     8221 atatggattc cgagcatcat tttcttgcgg tctatcttgc tacgtatgtt gatcttgacg
     8281 ctgtggatca agcaacgcca ctcgctcgct ccatcgcagg ctggtcgcag acaaattaaa
     8341 aggcggcaaa ctcgtacagc cgcggggttg tccgctgcaa agtacagagt gataaaagcc
     8401 gccatgcgac catcaacgcg ttgatgccca gctttttcga tccgagaatc caccgtagag
     8461 gcgatagcaa gtaaagaaaa gctaaacaaa aaaaaatttc tgcccctaag ccatgaaaac
     8521 gagatggggt ggagcagaac caaggaaaga gtcgcgctgg gctgccgttc cggaaggtgt
     8581 tgtaaaggct cgacgcccaa ggtgggagtc taggagaaga atttgcatcg ggagtggggc
     8641 gggttacccc tccatatcca atgacagata tctaccagcc aagggtttga gcccgcccgc
     8701 ttagtcgtcg tcctcgcttg cccctccata aaaggatttc ccctccccct cccacaaaat
     8761 tttctttccc ttcctctcct tgtccgcttc agtacgtata tcttcccttc cctcgcttct
     8821 ctcctccatc cttctttcat ccatctcctg ctaacttctc tgctcagcac ctctacgcat
     8881 tactagccgt agtatctgag cacttctccc ttttatattc cacaaaacat aacacaacct
     8941 tcaccatgga caagaagtat agcatcgggc tggacattgg aacgaactcg gttggttggg
     9001 ctgtgattac ggacgaatac aaggtgccat ccaagaagtt taaggtcctg ggaaacaccg
     9061 accgtcactc aatcaagaag aatctcattg gagccctgct cttcgatagt ggggagaccg
     9121 ccgaagctac tcgactgaag cgaacggctc gccggcgtta tacacgacgc aagaatcgca
     9181 tctgctacct ccaggagatt ttcagcaacg aaatggctaa ggttgatgac tcattctttc
     9241 atcgactcga agaaagtttc ttggtcgagg aggataagaa gcacgagcgc catccgatct
     9301 ttggtaacat tgtggatgag gttgcctatc acgaaaagta cccaactatc tatcatcttc
     9361 gtaagaagct ggtcgatagc acggacaagg ctgatttgcg acttatctac ctggcactcg
     9421 cgcacatgat taagttccgc ggccattttc ttatcgaggg tgacctgaac cccgataatt
     9481 ctgacgttga taagctcttc atccagttgg tccaaaccta caatcagctg tttgaggaaa
     9541 accctattaa tgcatctggc gtggacgcca aggctatcct ttcggcgcgc ctgtctaagt
     9601 cgcggcgttt ggagaacctt atcgcacaac tccccggcga aaagaagaac ggcctcttcg
     9661 gtaatttgat tgcgttgtca cttggtctga ctcctaactt caagagtaat tttgacctgg
     9721 cagaggatgc gaagctccag ttgtctaagg atacgtatga tgacgatctc gacaacttgc
     9781 ttgcccaaat cggtgaccag tacgctgatc ttttcctggc cgctaagaat ctctcagatg
     9841 caatcctgct cagtgacatt ttgcgggtca acaccgagat tactaaggcc cccctgtcag
     9901 ctagtatgat caagcggtat gatgagcacc atcaggacct caccttgctt aaggccctcg
     9961 tgcgtcagca attgcctgag aagtataagg aaatcttctt tgaccaatcc aagaacggat
    10021 acgcagggta tattgatggc ggtgcgagcc aggaggaatt ctacaagttt atcaagccga
    10081 ttttggagaa gatggacggc actgaggaac tgctcgtcaa gctgaatcgc gaagatttgc
    10141 ttcgtaagca acgaacgttc gacaacggct ccatcccgca ccagattcat ctgggcgagc
    10201 tccacgccat ccttcgacgc caggaagatt tctacccatt tctgaaggac aaccgtgaga
    10261 agatcgaaaa gattcttaca ttccgaatcc cctactatgt gggacctttg gcccgtggga
    10321 attcccgatt tgcttggatg acccgaaaga gcgaggaaac catcactccg tggaacttcg
    10381 aggaagtcgt ggacaagggt gcatccgcgc agagcttcat tgagcggatg accaattttg
    10441 ataagaacct tccgaatgaa aaggtcctgc caaagcattc gctgctctac gagtatttca
    10501 ccgtgtataa cgaactgact aaggtcaagt acgtgacgga gggaatgcgg aagccagcct
    10561 tcctctcagg ggaacaaaag aaggctatcg tcgatttgct ttttaagacc aatcgtaaag
    10621 tgactgttaa gcagctgaag gaggattatt tcaagaagat tgaatgtttc gactccgtcg
    10681 agatcagcgg cgtggaagat cgctttaacg cttccctcgg tacctaccac gacctgctca
    10741 agatcattaa ggacaaggat ttcctcgata acgaggaaaa tgaggacatc ttggaagata
    10801 ttgtcctcac gttgacactt tttgaggacc gcgaaatgat cgaggaacgg ctcaagacat
    10861 atgcccattt gttcgacgat aaggtgatga agcagctgaa gcggcgtcga tacaccggat
    10921 ggggtcgcct tagccggaag ctgatcaacg gcattcgaga taagcaatct ggtaagacta
    10981 tcttggattt ccttaagtcg gacggcttcg ccaaccgcaa ttttatgcag cttattcacg
    11041 acgattccct gacgttcaag gaggacatcc agaaggcaca agtctcagga caaggggatt
    11101 ccctgcacga gcatatcgcc aacctggctg gatccccggc gatcaagaag gggattcttc
    11161 agaccgtcaa ggttgtcgac gagctggtca aggtgatggg ccgtcataag ccagaaaaca
    11221 tcgtgattga gatggcccga gaaaatcaga ccactcaaaa gggtcagaag aacagccgcg
    11281 agcggatgaa gcggatcgag gaaggcatta aggaacttgg ttctcagatc ctgaaggagc
    11341 accctgttga aaacacacag ctccaaaatg agaagctgta tctctactat ttgcaaaatg
    11401 gacgcgacat gtacgtcgat caggagctcg acattaaccg gttgtcggac tacgatgttg
    11461 accatatcgt cccgcaatcc ttccttaagg acgatagcat tgataacaag gtgctgactc
    11521 gctcagataa gaaccggggc aagtccgaca atgttccaag cgaggaagtg gttaagaaga
    11581 tgaagaacta ctggcgccaa ttgcttaatg ccaagctcat cacacagcgc aagtttgaca
    11641 acttgaccaa ggccgagcgg ggagggctga gtgaactcga taaggctggc ttcatcaagc
    11701 gtcaactcgt ggagacgcga cagatcacaa agcacgttgc tcagattctg gactcccgga
    11761 tgaacacaaa gtacgacgag aatgataagc tcatccgtga agttaaggtc attaccctca
    11821 agtctaagtt ggtgtcggat ttccgcaagg acttccaatt ttataaggtt cgggagatca
    11881 acaattatca ccatgcacat gatgcgtacc tcaacgcagt cgtgggaact gcgctcatca
    11941 agaagtatcc caagttggag tccgaattcg tctacgggga ttataaggtt tacgacgtcc
    12001 gcaagatgat cgccaagagt gagcaggaaa ttggcaaggc cacggctaag tatttctttt
    12061 actccaacat catgaatttc tttaagacgg agatcacact cgccaatgga gaaatccgta
    12121 agcgaccttt gattgagacc aacggcgaga ctggtgaaat cgtttgggat aaggggcgcg
    12181 acttcgctac cgtgcggaag gttctgagca tgccgcaagt caatatcgtc aagaaaaccg
    12241 aggtgcagac aggcggtttc tctaaggaat cgattcttcc aaagcgtaac tctgacaagc
    12301 tgatcgctcg aaagaaggat tgggacccca agaagtatgg agggttcgat tctcctacag
    12361 tggcatactc ggttctcgtt gtcgcgaagg ttgagaaggg aaagtctaag aagctgaagt
    12421 cggtcaagga actgctcggg atcaccatta tggagcgctc cagcttcgaa aagaatccca
    12481 tcgactttct cgaggccaag ggctataagg aagtcaagaa ggatcttatc attaagctgc
    12541 ctaagtactc tttgttcgag cttgaaaacg gtcgaaagcg aatgctcgca tcggcaggag
    12601 agttgcagaa ggggaatgaa ttggcacttc cctcaaagta cgtgaacttc ctgtatctcg
    12661 cgtcccacta cgagaagctg aagggtagcc ctgaggacaa cgaacagaag caactttttg
    12721 ttgagcaaca caagcattat ctggatgaga tcattgaaca gatttcagag ttcagtaagc
    12781 gcgtcatcct cgccgatgct aatctcgaca aggtgttgtc ggcctacaac aagcaccgtg
    12841 acaagccgat ccgagagcag gctgaaaata tcattcatct gttcaccctc actaacttgg
    12901 gagcaccagc agcgttcaag tattttgata cgacaatcga ccgtaagcga tacacgtcca
    12961 caaaggaggt gcttgatgcg accctgattc atcaatccat cactgggctc tatgaaaccc
    13021 gtatcgacct tagtcaactg gggggcgacc ctcccaagaa gaagcgcaag gtctgagcgg
    13081 acattcgatt tatgccgtta tgacttcctt aaaaaagcct ttacgaatga aagaaatgga
    13141 attagacttg ttatgtagtt gattctacaa tggattatga ttcctgaact tcaaatccgc
    13201 tgttcattat taatctcagc tcttcccgta aagccaatgt tgaaactatt cgtaaatgta
    13261 cctcgttttg cgtgtacctt gcttatcacg tgatattaca tgacctggac agagttctgc
    13321 gcgaaagtca taacgtaaat cccgggcggt aggtgcgtcc cgggcggaag gtagttttct
    13381 cgtccacccc aacgcgttta tcaacctcaa ctttcaacaa ccatcatgcc accaaaagcg
    13441 cgtaaaacaa agcgagattt gattgagcaa gagggcagga tccaatgcgc gattcaagac
    13501 attaaaaatg gaaaatttca aaaaattgcg cccgcagcgc gtgcatacaa aattcatccc
    13561 aatacaagtg taattcctcg tgtactgtgt aagcgcccac taggtaatat gacatgatta
    13621 cgaattcgag ctcggtaccc ggccggggat cctctagacg tggagttacc agtgattgac
    13681 caatgtttta tcttctacag ttctgcctgt ctaccccatt ctagctgtac ctgactacag
    13741 agtagtttaa ttgtggttga ccccacagtc ggaggcggag gaatacagca ccgatgtggc
    13801 ctgtctccat ccagattggc acgcaatttt tacacgcgga aaagatcgag atagagtacg
    13861 actttaaatt tagtccccgg cggcttctat tttagaatat ttgagatttg attctcaagc
    13921 aattgatttg gttgggtcac cctcaattgg ataatatacc tcattgctcg gctacttcaa
    13981 ctcatcaatc accgtcatac cccgcatata accctccatt cccacgatgt cgtccaagtc
    14041 gcaattgact tacggtgctc gagccagcaa gcaccccaat cctctggcaa agagactttt
    14101 tgagattgcc gaagcaaaga agacaaacgt taccgtctct gctgatgtga cgacaacccg
    14161 agaactcctg gacctcgctg accgtacgga agctgttgga tccaatacat atgccgtcta
    14221 gcaatggact aatcaacttt tgatgataca ggtctcggtc cctacatcgc cgtcatcaag
    14281 acacacatcg acatcctcac cgatttcagc gtcgacacta tcaatggcct gaatgtgctg
    14341 gctcaaaagt acaacttttt gatcttcgag gaccgcaaat tcatcgacat cggcaatacc
    14401 gtccagaagc aataccacgg cggtgctctg aggatctccg aatgggccca cattatcaac
    14461 tgcagcgttc tccctggcga gggcatcgtc gaggctctgg cccagaccgc atctgcgcaa
    14521 gacttcccct atggtcctga gagaggactg ttggtcctgg cagagatgac ccccaaagga
    14581 tcgctggcta cgggcgagta taccaaggca tcggttgact acgctcgcaa atacaagaac
    14641 ttcgttatgg gtttcgtgtc gacgcgggcc ctgacggaag tgcagtcgga tgtgtcttca
    14701 gcctcggagg atgaagattt cgtggtcttc acgacgggtg tgaacctctc ttccaaagga
    14761 gataagcttg gacagcaata ccagactcct gcatcggcta ttggacgcgg tgccgacttt
    14821 atcatcgccg gtcgaggcat ctacgctgct cccgacccgg ttgaagctgc acagcggtac
    14881 cagaaagaag gctgggaagc ttatatggcc agagtatgcg gcaagtcatg atttcctctt
    14941 ggagcaaaag tgtagtgcca gtacgagtgt tgtggaggaa ggctgcatac attgtgcctg
    15001 tcattaaacg atgagctcgt ccgtattggc ccctgtaatg ccatgttttc cgcccccaat
    15061 cgtcaaggtt ttccctttgt tagattccta ccagtcatct agcaaggcgg ccgcagctag
    15121 cacaattgag gcatccccac taccgcatta agacctcagc gcggccgcaa atttaaataa
    15181 aatgaagtga agttcctata ctttctagag aataggaact tctatagtga gtcgaataag
    15241 ggcgacacaa aatttattct aaatgcataa taaatactga taacatctta tagtttgtat
    15301 tatattttgt attatcgttg acatgtataa ttttgatatc aaaaactgat tttcccttta
    15361 ttattttcga gatttatttt cttaattctc tttaacaaac tagaaatatt gtatatacaa
    15421 aaaatcataa ataatagatg aatagtttaa ttataggtgt tcatcaatcg aaaaagcaac
    15481 gtatcttatt taaagtgcgt tgcttttttc tcatttataa ggttaaataa ttctcatata
    15541 tcaagcaaag tgacaggcgc ccttaaatat tctgacaaat gctctttccc taaactcccc
    15601 ccataaaaaa acccgccgaa gcgggttttt acgttatttg cggattaacg attactcgtt
    15661 atcagaaccg cccagggggc ccgagcttaa gactggccgt cgttttacaa cacagaaaga
    15721 gtttgtagaa acgcaaaaag gccatccgtc aggggccttc tgcttagttt gatgcctggc
    15781 agttccctac tctcgccttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
    15841 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
    15901 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
    15961 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
    16021 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
    16081 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    16141 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
    16201 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
    16261 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    16321 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    16381 ttcttgaagt ggtgggctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
    16441 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    16501 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    16561 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgacgcgcgc
    16621 gtaactcacg ttaagggatt ttggtcatga gcttgcgccg tcccgtcaag tcagcgtaat
    16681 gctctgctt
//