pcDNA-dCas9-VP64

价格:1200元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

买家导航

载体基本信息

质粒名称: pcDNA-dCas9-VP64 
出品公司: 爱迪基因
目录编号: 47107
存储实验室: Charles Gersbach

载体骨架基本信息
载体名称: pcDNA3.1
载体出品公司: Invitrogen
载体抗性: 氨苄霉素
完整质粒大小: 9812 bp
载体类型: 哺乳细胞表达
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: dCas9-VP64
物种来源: S. Pyogenes
插入大小: 4446 bp
基因突变: D10A, H840A (catalytically inactive)
启动子 CMV
标签/融合蛋白: Flag(N端)         SV40 NLS(N端)         SV40 NLS(C端)          VP64(C端)         HA(C端)
克隆方法: 未知
5' 测序引物 CMV-F
3' 测序引物 BGH-rev

文献引用方法
材料和方法部分: pcDNA-dCas9-VP64 was a gift from Vladislav Verkhusha (Addgene plasmid # 45465)
参考文献部分:
Near-infrared fluorescent proteins for multicolor in vivo imaging. Shcherbakova DM, Verkhusha VV. Nat Methods. 2013 Jun 16. doi: 10.1038/nmeth.2521. 10.1038/nmeth.2521 PubMed 23770755

载体图谱质粒图谱和多克隆位点信息

pcDNA-dCas9-VP64载体图谱

载体简介

载体序列

LOCUS       pcDNA-dCas9-VP64	9812 bp 	DNA	SYN
DEFINITION  pcDNA-dCas9-VP64
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..9812
                     /organism="pcDNA-dCas9-VP64"
                     /mol_type="other DNA"
     promoter        236..852
                     /label="CMV_immearly_promoter"
     misc_feature    315..602
                     /label="CAG_enhancer"
     misc_feature    769..789
                     /label="CMV_fwd_primer"
     promoter        863..881
                     /label="T7_promoter"
     CDS             904..5355
                     /label="ORF frame 1"
     misc_feature    907..927
                     /label="FLAG"
     misc_feature    928..948
                     /label="FLAG"
     misc_feature    949..972
                     /label="FLAG"
     misc_feature    958..972
                     /label="EK"
     misc_feature    979..1002
                     /label="SV40 NLS"
     misc_feature    979..999
                     /label="SV40 NLS"
     misc_feature    985..999
                     /label="NLS"
     misc_feature    5323..5349
                     /label="HA"
     misc_feature    complement(5406..5423)
                     /label="BGH_rev_primer"
     terminator      5409..5636
                     /label="bGH_PA_terminator"
     rep_origin      5699..6005
                     /label="f1_origin"
     misc_feature    complement(6119..6139)
                     /label="pBABE_3_primer"
     misc_feature    complement(6125..6340)
                     /label="SV40_enhancer"
     promoter        6137..6405
                     /label="SV40_promoter"
     rep_origin      6304..6381
                     /label="SV40_origin"
     misc_feature    6366..6385
                     /label="SV40pro_F_primer"
     CDS             6520..7314
                     /label="ORF frame 1"
     gene            6523..7311
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
     CDS             complement(6829..7365)
                     /label="ORF frame 3"
     terminator      7491..7610
                     /label="SV40_PA_terminator"
     misc_feature    7579..7598
                     /label="EBV_rev_primer"
     promoter        complement(7654..7672)
                     /label="M13_reverse_primer"
     misc_feature    complement(7671..7693)
                     /label="M13_pUC_rev_primer"
     promoter        complement(7707..7736)
                     /label="lac_promoter"
     gene            complement(8816..9676)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(8816..9676)
                     /label="ORF frame 2"
     promoter        complement(9718..9746)
                     /label="AmpR_promoter"
ORIGIN
    1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG
   61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG
  121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC
  181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT
  241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA
  301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC
  361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC
  421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT
  481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT
  541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA
  601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG
  661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC
  721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG
  781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA
  841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGctagC
  901 GCCATGGACT ACAAAGACCA TGACGGTGAT TATAAAGATC ATGACATCGA TTACAAGGAT
  961 GACGATGACA AGatggcccc caagaagaag aggaaggtgg gccgcggaat gGATAAGAAA
 1021 TACTCAATAG GCTTAGCTAT CGGCACAAAT AGCGTCGGAT GGGCGGTGAT CACTGATGAA
 1081 TATAAGGTTC CGTCTAAAAA GTTCAAGGTT CTGGGAAATA CAGACCGCCA CAGTATCAAA
 1141 AAAAATCTTA TAGGGGCTCT TTTATTTGAC AGTGGAGAGA CAGCGGAAGC GACTCGTCTC
 1201 AAACGGACAG CTCGTAGAAG GTATACACGT CGGAAGAATC GTATTTGTTA TCTACAGGAG
 1261 ATTTTTTCAA ATGAGATGGC GAAAGTAGAT GATAGTTTCT TTCATCGACT TGAAGAGTCT
 1321 TTTTTGGTGG AAGAAGACAA GAAGCATGAA CGTCATCCTA TTTTTGGAAA TATAGTAGAT
 1381 GAAGTTGCTT ATCATGAGAA ATATCCAACT ATCTATCATC TGCGAAAAAA ATTGGTAGAT
 1441 TCTACTGATA AAGCGGATTT GCGCTTAATC TATTTGGCCT TAGCGCATAT GATTAAGTTT
 1501 CGTGGTCATT TTTTGATTGA GGGAGATTTA AATCCTGATA ATAGTGATGT GGACAAACTA
 1561 TTTATCCAGT TGGTACAAAC CTACAATCAA TTATTTGAAG AAAACCCTAT TAACGCAAGT
 1621 GGAGTAGATG CTAAAGCGAT TCTTTCTGCA CGATTGAGTA AATCAAGACG ATTAGAAAAT
 1681 CTCATTGCTC AGCTCCCCGG TGAGAAGAAA AATGGCTTAT TTGGGAATCT CATTGCTTTG
 1741 TCATTGGGTT TGACCCCTAA TTTTAAATCA AATTTTGATT TGGCAGAAGA TGCTAAATTA
 1801 CAGCTTTCAA AAGATACTTA CGATGATGAT TTAGATAATT TATTGGCGCA AATTGGAGAT
 1861 CAATATGCTG ATTTGTTTTT GGCAGCTAAG AATTTATCAG ATGCTATTTT ACTTTCAGAT
 1921 ATCCTAAGAG TAAATACTGA AATAACTAAG GCTCCCCTAT CAGCTTCAAT GATTAAACGC
 1981 TACGATGAAC ATCATCAAGA CTTGACTCTT TTAAAAGCTT TAGTTCGACA ACAACTTCCA
 2041 GAAAAGTATA AAGAAATCTT TTTTGATCAA TCAAAAAACG GATATGCAGG TTATATTGAT
 2101 GGGGGAGCTA GCCAAGAAGA ATTTTATAAA TTTATCAAAC CAATTTTAGA AAAAATGGAT
 2161 GGTACTGAGG AATTATTGGT GAAACTAAAT CGTGAAGATT TGCTGCGCAA GCAACGGACC
 2221 TTTGACAACG GCTCTATTCC CCATCAAATT CACTTGGGTG AGCTGCATGC TATTTTGAGA
 2281 AGACAAGAAG ACTTTTATCC ATTTTTAAAA GACAATCGTG AGAAGATTGA AAAAATCTTG
 2341 ACTTTTCGAA TTCCTTATTA TGTTGGTCCA TTGGCGCGTG GCAATAGTCG TTTTGCATGG
 2401 ATGACTCGGA AGTCTGAAGA AACAATTACC CCATGGAATT TTGAAGAAGT TGTCGATAAA
 2461 GGTGCTTCAG CTCAATCATT TATTGAACGC ATGACAAACT TTGATAAAAA TCTTCCAAAT
 2521 GAAAAAGTAC TACCAAAACA TAGTTTGCTT TATGAGTATT TTACGGTTTA TAACGAATTG
 2581 ACAAAGGTCA AATATGTTAC TGAAGGAATG CGAAAACCAG CATTTCTTTC AGGTGAACAG
 2641 AAGAAAGCCA TTGTTGATTT ACTCTTCAAA ACAAATCGAA AAGTAACCGT TAAGCAATTA
 2701 AAAGAAGATT ATTTCAAAAA AATAGAATGT TTTGATAGTG TTGAAATTTC AGGAGTTGAA
 2761 GATAGATTTA ATGCTTCATT AGGTACCTAC CATGATTTGC TAAAAATTAT TAAAGATAAA
 2821 GATTTTTTGG ATAATGAAGA AAATGAAGAT ATCTTAGAGG ATATTGTTTT AACATTGACC
 2881 TTATTTGAAG ATAGGGAGAT GATTGAGGAA AGACTTAAAA CATATGCTCA CCTCTTTGAT
 2941 GATAAGGTGA TGAAACAGCT TAAACGTCGC CGTTATACTG GTTGGGGACG TTTGTCTCGA
 3001 AAATTGATTA ATGGTATTAG GGATAAGCAA TCTGGCAAAA CAATATTAGA TTTTTTGAAA
 3061 TCAGATGGTT TTGCCAATCG CAATTTTATG CAGCTGATCC ATGATGATAG TTTGACATTT
 3121 AAAGAAGACA TTCAAAAAGC ACAAGTGTCT GGACAAGGCG ATAGTTTACA TGAACATATT
 3181 GCAAATTTAG CTGGTAGCCC TGCTATTAAA AAAGGTATTT TACAGACTGT AAAAGTTGTT
 3241 GATGAATTGG TCAAAGTAAT GGGGCGGCAT AAGCCAGAAA ATATCGTTAT TGAAATGGCA
 3301 CGTGAAAATC AGACAACTCA AAAGGGCCAG AAAAATTCGC GAGAGCGTAT GAAACGAATC
 3361 GAAGAAGGTA TCAAAGAATT AGGAAGTCAG ATTCTTAAAG AGCATCCTGT TGAAAATACT
 3421 CAATTGCAAA ATGAAAAGCT CTATCTCTAT TATCTCCAAA ATGGAAGAGA CATGTATGTG
 3481 GACCAAGAAT TAGATATTAA TCGTTTAAGT GATTATGATG TCGATGCCAT TGTTCCACAA
 3541 AGTTTCCTTA AAGACGATTC AATAGACAAT AAGGTCTTAA CGCGTTCTGA TAAAAATCGT
 3601 GGTAAATCGG ATAACGTTCC AAGTGAAGAA GTAGTCAAAA AGATGAAAAA CTATTGGAGA
 3661 CAACTTCTAA ACGCCAAGTT AATCACTCAA CGTAAGTTTG ATAATTTAAC GAAAGCTGAA
 3721 CGTGGAGGTT TGAGTGAACT TGATAAAGCT GGTTTTATCA AACGCCAATT GGTTGAAACT
 3781 CGCCAAATCA CTAAGCATGT GGCACAAATT TTGGATAGTC GCATGAATAC TAAATACGAT
 3841 GAAAATGATA AACTTATTCG AGAGGTTAAA GTGATTACCT TAAAATCTAA ATTAGTTTCT
 3901 GACTTCCGAA AAGATTTCCA ATTCTATAAA GTACGTGAGA TTAACAATTA CCATCATGCC
 3961 CATGATGCGT ATCTAAATGC CGTCGTTGGA ACTGCTTTGA TTAAGAAATA TCCAAAACTT
 4021 GAATCGGAGT TTGTCTATGG TGATTATAAA GTTTATGATG TTCGTAAAAT GATTGCTAAG
 4081 TCTGAGCAAG AAATAGGCAA AGCAACCGCA AAATATTTCT TTTACTCTAA TATCATGAAC
 4141 TTCTTCAAAA CAGAAATTAC ACTTGCAAAT GGAGAGATTC GCAAACGCCC TCTAATCGAA
 4201 ACTAATGGGG AAACTGGAGA AATTGTCTGG GATAAAGGGC GAGATTTTGC CACAGTGCGC
 4261 AAAGTATTGT CCATGCCCCA AGTCAATATT GTCAAGAAAA CAGAAGTACA GACAGGCGGA
 4321 TTCTCCAAGG AGTCAATTTT ACCAAAAAGA AATTCGGACA AGCTTATTGC TCGTAAAAAA
 4381 GACTGGGATC CAAAAAAATA TGGTGGTTTT GATAGTCCAA CGGTAGCTTA TTCAGTCCTA
 4441 GTGGTTGCTA AGGTGGAAAA AGGGAAATCG AAGAAGTTAA AATCCGTTAA AGAGTTACTA
 4501 GGGATCACAA TTATGGAAAG AAGTTCCTTT GAAAAAAATC CGATTGACTT TTTAGAAGCT
 4561 AAAGGATATA AGGAAGTTAA AAAAGACTTA ATCATTAAAC TACCTAAATA TAGTCTTTTT
 4621 GAGTTAGAAA ACGGTCGTAA ACGGATGCTG GCTAGTGCCG GAGAATTACA AAAAGGAAAT
 4681 GAGCTGGCTC TGCCAAGCAA ATATGTGAAT TTTTTATATT TAGCTAGTCA TTATGAAAAG
 4741 TTGAAGGGTA GTCCAGAAGA TAACGAACAA AAACAATTGT TTGTGGAGCA GCATAAGCAT
 4801 TATTTAGATG AGATTATTGA GCAAATCAGT GAATTTTCTA AGCGTGTTAT TTTAGCAGAT
 4861 GCCAATTTAG ATAAAGTTCT TAGTGCATAT AACAAACATA GAGACAAACC AATACGTGAA
 4921 CAAGCAGAAA ATATTATTCA TTTATTTACG TTGACGAATC TTGGAGCTCC CGCTGCTTTT
 4981 AAATATTTTG ATACAACAAT TGATCGTAAA CGATATACGT CTACAAAAGA AGTTTTAGAT
 5041 GCCACTCTTA TCCATCAATC CATCACTGGT CTTTATGAAA CACGCATTGA TTTGAGTCAG
 5101 CTAGGAGgtg acccaattgc cGgatcCaAG GCTAGCCCGA AAAAGAAACG CAAAGTTGGG
 5161 CGCGCCGACG CGCTGGACGA TTTCGATCTC GACATGCTGG GTTCTGATGC CCTCGATGAC
 5221 TTTGACCTGG ATATGTTGGG AAGCGACGCA TTGGATGACT TTGATCTGGA CATGCTCGGC
 5281 TCCGATGCTC TGGACGATTT CGATCTCGAT ATGTTAATTA ACTACCCGTA CGACGTTCCG
 5341 GACTACGCTT CTTGAATCGG TAGGAATTCG CGGCCGTcta gACttaaGTT TAAACCGCTG
 5401 ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT GTTTGCCCCT CCCCCGTGCC
 5461 TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC TAATAAAATG AGGAAATTGC
 5521 ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT GGGGTGGGGC AGGACAGCAA
 5581 GGGGGAGGAT TGGGAAGACA ATAGCAGGCA TGCTGGGGAT GCGGTGGGCT CTATGGCTTC
 5641 TGAGGCGGAA AGAACCAGCT GGGGCTCTAG GGGGTATCCC CACGCGCCCT GTAGCGGCGC
 5701 ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT
 5761 AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG
 5821 TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA
 5881 CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT
 5941 TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG
 6001 AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC
 6061 GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT AATTCTGTGG
 6121 AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA
 6181 AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC
 6241 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG
 6301 CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT
 6361 TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCTGCCTCTG AGCTATTCCA GAAGTAGTGA
 6421 GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT
 6481 TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG
 6541 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG
 6601 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT
 6661 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA
 6721 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG
 6781 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT
 6841 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT
 6901 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG
 6961 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA
 7021 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC
 7081 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC
 7141 GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT
 7201 ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC
 7261 GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA
 7321 CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT
 7381 CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA
 7441 TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG
 7501 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT
 7561 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA
 7621 TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA
 7681 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT
 7741 GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC
 7801 AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG
 7861 GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC
 7921 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG
 7981 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA
 8041 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC
 8101 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC
 8161 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG
 8221 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT
 8281 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC
 8341 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC
 8401 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG
 8461 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG
 8521 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA
 8581 CCACCGCTGG TAGCGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT
 8641 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC
 8701 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT
 8761 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC
 8821 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG
 8881 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG
 8941 CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC
 9001 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA
 9061 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG
 9121 TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT
 9181 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA
 9241 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG
 9301 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA
 9361 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT
 9421 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA
 9481 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT
 9541 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT
 9601 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA
 9661 AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT
 9721 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC
 9781 GCACATTTCC CCGAAAAGTG CCACCTGACG TC
//

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