质粒名称: | pcDNA-dCas9-VP64 |
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出品公司: | 爱迪基因 |
目录编号: | 47107 |
存储实验室: | Charles Gersbach |
载体骨架基本信息 |
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载体名称: | pcDNA3.1 |
载体出品公司: | Invitrogen |
载体抗性: | 氨苄霉素 |
完整质粒大小: | 9812 bp |
载体类型: | 哺乳细胞表达 |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | dCas9-VP64 |
物种来源: | S. Pyogenes |
插入大小: | 4446 bp |
基因突变: | D10A, H840A (catalytically inactive) |
启动子 | CMV |
标签/融合蛋白: | Flag(N端) SV40 NLS(N端) SV40 NLS(C端) VP64(C端) HA(C端) |
克隆方法: | 未知 |
5' 测序引物 | CMV-F |
3' 测序引物 | BGH-rev |
文献引用方法 |
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材料和方法部分: | pcDNA-dCas9-VP64 was a gift from Vladislav Verkhusha (Addgene plasmid # 45465) |
参考文献部分: |
Near-infrared fluorescent proteins for multicolor in vivo imaging. Shcherbakova DM, Verkhusha VV. Nat Methods. 2013 Jun 16. doi: 10.1038/nmeth.2521. 10.1038/nmeth.2521 PubMed 23770755 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pcDNA-dCas9-VP64 9812 bp DNA SYN DEFINITION pcDNA-dCas9-VP64 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..9812 /organism="pcDNA-dCas9-VP64" /mol_type="other DNA" promoter 236..852 /label="CMV_immearly_promoter" misc_feature 315..602 /label="CAG_enhancer" misc_feature 769..789 /label="CMV_fwd_primer" promoter 863..881 /label="T7_promoter" CDS 904..5355 /label="ORF frame 1" misc_feature 907..927 /label="FLAG" misc_feature 928..948 /label="FLAG" misc_feature 949..972 /label="FLAG" misc_feature 958..972 /label="EK" misc_feature 979..1002 /label="SV40 NLS" misc_feature 979..999 /label="SV40 NLS" misc_feature 985..999 /label="NLS" misc_feature 5323..5349 /label="HA" misc_feature complement(5406..5423) /label="BGH_rev_primer" terminator 5409..5636 /label="bGH_PA_terminator" rep_origin 5699..6005 /label="f1_origin" misc_feature complement(6119..6139) /label="pBABE_3_primer" misc_feature complement(6125..6340) /label="SV40_enhancer" promoter 6137..6405 /label="SV40_promoter" rep_origin 6304..6381 /label="SV40_origin" misc_feature 6366..6385 /label="SV40pro_F_primer" CDS 6520..7314 /label="ORF frame 1" gene 6523..7311 /label="NeoR/KanR" /gene="NeoR/KanR" CDS complement(6829..7365) /label="ORF frame 3" terminator 7491..7610 /label="SV40_PA_terminator" misc_feature 7579..7598 /label="EBV_rev_primer" promoter complement(7654..7672) /label="M13_reverse_primer" misc_feature complement(7671..7693) /label="M13_pUC_rev_primer" promoter complement(7707..7736) /label="lac_promoter" gene complement(8816..9676) /label="Ampicillin" /gene="Ampicillin" CDS complement(8816..9676) /label="ORF frame 2" promoter complement(9718..9746) /label="AmpR_promoter" ORIGIN 1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGctagC 901 GCCATGGACT ACAAAGACCA TGACGGTGAT TATAAAGATC ATGACATCGA TTACAAGGAT 961 GACGATGACA AGatggcccc caagaagaag aggaaggtgg gccgcggaat gGATAAGAAA 1021 TACTCAATAG GCTTAGCTAT CGGCACAAAT AGCGTCGGAT GGGCGGTGAT CACTGATGAA 1081 TATAAGGTTC CGTCTAAAAA GTTCAAGGTT CTGGGAAATA CAGACCGCCA CAGTATCAAA 1141 AAAAATCTTA TAGGGGCTCT TTTATTTGAC AGTGGAGAGA CAGCGGAAGC GACTCGTCTC 1201 AAACGGACAG CTCGTAGAAG GTATACACGT CGGAAGAATC GTATTTGTTA TCTACAGGAG 1261 ATTTTTTCAA ATGAGATGGC GAAAGTAGAT GATAGTTTCT TTCATCGACT TGAAGAGTCT 1321 TTTTTGGTGG AAGAAGACAA GAAGCATGAA CGTCATCCTA TTTTTGGAAA TATAGTAGAT 1381 GAAGTTGCTT ATCATGAGAA ATATCCAACT ATCTATCATC TGCGAAAAAA ATTGGTAGAT 1441 TCTACTGATA AAGCGGATTT GCGCTTAATC TATTTGGCCT TAGCGCATAT GATTAAGTTT 1501 CGTGGTCATT TTTTGATTGA GGGAGATTTA AATCCTGATA ATAGTGATGT GGACAAACTA 1561 TTTATCCAGT TGGTACAAAC CTACAATCAA TTATTTGAAG AAAACCCTAT TAACGCAAGT 1621 GGAGTAGATG CTAAAGCGAT TCTTTCTGCA CGATTGAGTA AATCAAGACG ATTAGAAAAT 1681 CTCATTGCTC AGCTCCCCGG TGAGAAGAAA AATGGCTTAT TTGGGAATCT CATTGCTTTG 1741 TCATTGGGTT TGACCCCTAA TTTTAAATCA AATTTTGATT TGGCAGAAGA TGCTAAATTA 1801 CAGCTTTCAA AAGATACTTA CGATGATGAT TTAGATAATT TATTGGCGCA AATTGGAGAT 1861 CAATATGCTG ATTTGTTTTT GGCAGCTAAG AATTTATCAG ATGCTATTTT ACTTTCAGAT 1921 ATCCTAAGAG TAAATACTGA AATAACTAAG GCTCCCCTAT CAGCTTCAAT GATTAAACGC 1981 TACGATGAAC ATCATCAAGA CTTGACTCTT TTAAAAGCTT TAGTTCGACA ACAACTTCCA 2041 GAAAAGTATA AAGAAATCTT TTTTGATCAA TCAAAAAACG GATATGCAGG TTATATTGAT 2101 GGGGGAGCTA GCCAAGAAGA ATTTTATAAA TTTATCAAAC CAATTTTAGA AAAAATGGAT 2161 GGTACTGAGG AATTATTGGT GAAACTAAAT CGTGAAGATT TGCTGCGCAA GCAACGGACC 2221 TTTGACAACG GCTCTATTCC CCATCAAATT CACTTGGGTG AGCTGCATGC TATTTTGAGA 2281 AGACAAGAAG ACTTTTATCC ATTTTTAAAA GACAATCGTG AGAAGATTGA AAAAATCTTG 2341 ACTTTTCGAA TTCCTTATTA TGTTGGTCCA TTGGCGCGTG GCAATAGTCG TTTTGCATGG 2401 ATGACTCGGA AGTCTGAAGA AACAATTACC CCATGGAATT TTGAAGAAGT TGTCGATAAA 2461 GGTGCTTCAG CTCAATCATT TATTGAACGC ATGACAAACT TTGATAAAAA TCTTCCAAAT 2521 GAAAAAGTAC TACCAAAACA TAGTTTGCTT TATGAGTATT TTACGGTTTA TAACGAATTG 2581 ACAAAGGTCA AATATGTTAC TGAAGGAATG CGAAAACCAG CATTTCTTTC AGGTGAACAG 2641 AAGAAAGCCA TTGTTGATTT ACTCTTCAAA ACAAATCGAA AAGTAACCGT TAAGCAATTA 2701 AAAGAAGATT ATTTCAAAAA AATAGAATGT TTTGATAGTG TTGAAATTTC AGGAGTTGAA 2761 GATAGATTTA ATGCTTCATT AGGTACCTAC CATGATTTGC TAAAAATTAT TAAAGATAAA 2821 GATTTTTTGG ATAATGAAGA AAATGAAGAT ATCTTAGAGG ATATTGTTTT AACATTGACC 2881 TTATTTGAAG ATAGGGAGAT GATTGAGGAA AGACTTAAAA CATATGCTCA CCTCTTTGAT 2941 GATAAGGTGA TGAAACAGCT TAAACGTCGC CGTTATACTG GTTGGGGACG TTTGTCTCGA 3001 AAATTGATTA ATGGTATTAG GGATAAGCAA TCTGGCAAAA CAATATTAGA TTTTTTGAAA 3061 TCAGATGGTT TTGCCAATCG CAATTTTATG CAGCTGATCC ATGATGATAG TTTGACATTT 3121 AAAGAAGACA TTCAAAAAGC ACAAGTGTCT GGACAAGGCG ATAGTTTACA TGAACATATT 3181 GCAAATTTAG CTGGTAGCCC TGCTATTAAA AAAGGTATTT TACAGACTGT AAAAGTTGTT 3241 GATGAATTGG TCAAAGTAAT GGGGCGGCAT AAGCCAGAAA ATATCGTTAT TGAAATGGCA 3301 CGTGAAAATC AGACAACTCA AAAGGGCCAG AAAAATTCGC GAGAGCGTAT GAAACGAATC 3361 GAAGAAGGTA TCAAAGAATT AGGAAGTCAG ATTCTTAAAG AGCATCCTGT TGAAAATACT 3421 CAATTGCAAA ATGAAAAGCT CTATCTCTAT TATCTCCAAA ATGGAAGAGA CATGTATGTG 3481 GACCAAGAAT TAGATATTAA TCGTTTAAGT GATTATGATG TCGATGCCAT TGTTCCACAA 3541 AGTTTCCTTA AAGACGATTC AATAGACAAT AAGGTCTTAA CGCGTTCTGA TAAAAATCGT 3601 GGTAAATCGG ATAACGTTCC AAGTGAAGAA GTAGTCAAAA AGATGAAAAA CTATTGGAGA 3661 CAACTTCTAA ACGCCAAGTT AATCACTCAA CGTAAGTTTG ATAATTTAAC GAAAGCTGAA 3721 CGTGGAGGTT TGAGTGAACT TGATAAAGCT GGTTTTATCA AACGCCAATT GGTTGAAACT 3781 CGCCAAATCA CTAAGCATGT GGCACAAATT TTGGATAGTC GCATGAATAC TAAATACGAT 3841 GAAAATGATA AACTTATTCG AGAGGTTAAA GTGATTACCT TAAAATCTAA ATTAGTTTCT 3901 GACTTCCGAA AAGATTTCCA ATTCTATAAA GTACGTGAGA TTAACAATTA CCATCATGCC 3961 CATGATGCGT ATCTAAATGC CGTCGTTGGA ACTGCTTTGA TTAAGAAATA TCCAAAACTT 4021 GAATCGGAGT TTGTCTATGG TGATTATAAA GTTTATGATG TTCGTAAAAT GATTGCTAAG 4081 TCTGAGCAAG AAATAGGCAA AGCAACCGCA AAATATTTCT TTTACTCTAA TATCATGAAC 4141 TTCTTCAAAA CAGAAATTAC ACTTGCAAAT GGAGAGATTC GCAAACGCCC TCTAATCGAA 4201 ACTAATGGGG AAACTGGAGA AATTGTCTGG GATAAAGGGC GAGATTTTGC CACAGTGCGC 4261 AAAGTATTGT CCATGCCCCA AGTCAATATT GTCAAGAAAA CAGAAGTACA GACAGGCGGA 4321 TTCTCCAAGG AGTCAATTTT ACCAAAAAGA AATTCGGACA AGCTTATTGC TCGTAAAAAA 4381 GACTGGGATC CAAAAAAATA TGGTGGTTTT GATAGTCCAA CGGTAGCTTA TTCAGTCCTA 4441 GTGGTTGCTA AGGTGGAAAA AGGGAAATCG AAGAAGTTAA AATCCGTTAA AGAGTTACTA 4501 GGGATCACAA TTATGGAAAG AAGTTCCTTT GAAAAAAATC CGATTGACTT TTTAGAAGCT 4561 AAAGGATATA AGGAAGTTAA AAAAGACTTA ATCATTAAAC TACCTAAATA TAGTCTTTTT 4621 GAGTTAGAAA ACGGTCGTAA ACGGATGCTG GCTAGTGCCG GAGAATTACA AAAAGGAAAT 4681 GAGCTGGCTC TGCCAAGCAA ATATGTGAAT TTTTTATATT TAGCTAGTCA TTATGAAAAG 4741 TTGAAGGGTA GTCCAGAAGA TAACGAACAA AAACAATTGT TTGTGGAGCA GCATAAGCAT 4801 TATTTAGATG AGATTATTGA GCAAATCAGT GAATTTTCTA AGCGTGTTAT TTTAGCAGAT 4861 GCCAATTTAG ATAAAGTTCT TAGTGCATAT AACAAACATA GAGACAAACC AATACGTGAA 4921 CAAGCAGAAA ATATTATTCA TTTATTTACG TTGACGAATC TTGGAGCTCC CGCTGCTTTT 4981 AAATATTTTG ATACAACAAT TGATCGTAAA CGATATACGT CTACAAAAGA AGTTTTAGAT 5041 GCCACTCTTA TCCATCAATC CATCACTGGT CTTTATGAAA CACGCATTGA TTTGAGTCAG 5101 CTAGGAGgtg acccaattgc cGgatcCaAG GCTAGCCCGA AAAAGAAACG CAAAGTTGGG 5161 CGCGCCGACG CGCTGGACGA TTTCGATCTC GACATGCTGG GTTCTGATGC CCTCGATGAC 5221 TTTGACCTGG ATATGTTGGG AAGCGACGCA TTGGATGACT TTGATCTGGA CATGCTCGGC 5281 TCCGATGCTC TGGACGATTT CGATCTCGAT ATGTTAATTA ACTACCCGTA CGACGTTCCG 5341 GACTACGCTT CTTGAATCGG TAGGAATTCG CGGCCGTcta gACttaaGTT TAAACCGCTG 5401 ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT GTTTGCCCCT CCCCCGTGCC 5461 TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC TAATAAAATG AGGAAATTGC 5521 ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT GGGGTGGGGC AGGACAGCAA 5581 GGGGGAGGAT TGGGAAGACA ATAGCAGGCA TGCTGGGGAT GCGGTGGGCT CTATGGCTTC 5641 TGAGGCGGAA AGAACCAGCT GGGGCTCTAG GGGGTATCCC CACGCGCCCT GTAGCGGCGC 5701 ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT 5761 AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG 5821 TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA 5881 CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT 5941 TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG 6001 AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC 6061 GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT AATTCTGTGG 6121 AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 6181 AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 6241 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG 6301 CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT 6361 TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCTGCCTCTG AGCTATTCCA GAAGTAGTGA 6421 GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT 6481 TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG 6541 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG 6601 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT 6661 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA 6721 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG 6781 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT 6841 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT 6901 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG 6961 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA 7021 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC 7081 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC 7141 GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT 7201 ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC 7261 GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA 7321 CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT 7381 CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA 7441 TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG 7501 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT 7561 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA 7621 TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA 7681 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT 7741 GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC 7801 AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG 7861 GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC 7921 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG 7981 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA 8041 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC 8101 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC 8161 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG 8221 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT 8281 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC 8341 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC 8401 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG 8461 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG 8521 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA 8581 CCACCGCTGG TAGCGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT 8641 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC 8701 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT 8761 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC 8821 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG 8881 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG 8941 CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC 9001 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA 9061 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG 9121 TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT 9181 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA 9241 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG 9301 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA 9361 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT 9421 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA 9481 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT 9541 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT 9601 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA 9661 AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT 9721 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC 9781 GCACATTTCC CCGAAAAGTG CCACCTGACG TC //